Behavior of gingival fibroblasts on titanium implant surfaces in combination with either Injectable-PRF or PRP

  • Various strategies have been employed to speed tissue regeneration using bioactive molecules. Interestingly, platelet concentrates derived from a patient’s own blood have been utilized as a regenerative strategy in recent years. In the present study, a novel liquid platelet formulation prepared without the use of anti-coagulants (injectable-platelet-rich fibrin, i-PRF) was compared to standard platelet-rich plasma (PRP) with gingival fibroblasts cultured on smooth and roughened titanium implant surfaces. Standard PRP and i-PRF (centrifuged at 700 rpm (60× g) for 3 min) were compared by assays for fibroblast biocompatibility, migration, adhesion, proliferation, as well as expression of platelet-derived growth factor (PDGF), transforming growth factor-β (TGF-β), collagen1 (COL1) and fibronectin (FN). The results demonstrate that i-PRF induced significantly higher cell migration, as well as higher messenger RNA (mRNA) levels of PDGF, TGF-β, collagen1 and fibronectin when compared to PRP. Furthermore, collagen1 synthesis was highest in the i-PRF group. These findings demonstrate that liquid platelet concentrates can be formulated without the use of anticoagulants and present much translational potential for future research. Future animal and clinical trials are now necessary to further investigate the potential of utilizing i-PRF for soft tissue regenerative protocols in combination with various biomaterials.

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Metadaten
Author:Xuzhu Wang, Yufeng Zhang, Joseph Choukroun, Shahram Michael GhanaatiORCiDGND, Richard J. Miron
URN:urn:nbn:de:hebis:30:3-563209
DOI:https://doi.org/10.3390/ijms18020331
ISSN:1422-0067
ISSN:1661-6596
Parent Title (English):International journal of molecular sciences
Document Type:Article
Language:English
Date of Publication (online):2017/02/04
Date of first Publication:2017/02/04
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/10/22
Tag:blood; fibrin; fibroblasts; platelet-rich fibrin; platelets; regeneration; wound healing
Volume:18
Issue:331
HeBIS-PPN:472867571
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0