Co-expression of CD34, CD90, OV-6 and cell-surface vimentin defines cancer stem cells of hepatoblastoma, which are affected by Hsp90 inhibitor 17-AAG

  • Cancer stem cells (CSCs) are nowadays one of the major focuses in tumor research since this subpopulation was revealed to be a great obstacle for successful treatment. The identification of CSCs in pediatric solid tumors harbors major challenges because of the immature character of these tumors. Here, we present CD34, CD90, OV-6 and cell-surface vimentin (csVimentin) as reliable markers to identify CSCs in hepatoblastoma cell lines. We were able to identify CSC characteristics for the subset of CD34+CD90+OV-6+csVimentin+-co-expressing cells, such as pluripotency, self-renewal, increased expression of EMT markers and migration. Treatment with Cisplatin as the standard chemotherapeutic drug in hepatoblastoma therapy further revealed the chemo-resistance of this subset, which is a main characteristic of CSCs. When we treated the cells with the Hsp90 inhibitor 17-AAG, we observed a significant reduction in the CSC subset. With our study, we identified CSCs of hepatoblastoma using CD34, CD90, OV-6 and csVimentin. This set of markers could be helpful to estimate the success of novel therapeutic approaches, as resistant CSCs are responsible for tumor relapses.

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Author:Mieun Lee-TheilenORCiDGND, Julia R. Hadhoud, Giulietta VolanteGND, Delaine D. Fadini, Julia Eichhorn, Udo RolleORCiDGND, Henning C. Fiege
Parent Title (English):Cells
Place of publication:Basel
Document Type:Article
Date of Publication (online):2021/09/29
Date of first Publication:2021/09/29
Publishing Institution:Universit├Ątsbibliothek Johann Christian Senckenberg
Release Date:2022/01/25
Tag:17-AAG; CSC marker; Hsp90 inhibitor; cancer stem cells; hepatoblastoma
Issue:10, art. 2598
Page Number:16
First Page:1
Last Page:16
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0