Determination of the effective dose of bone marrow mononuclear cell therapy for bone healing in vivo

  • Introduction: Cell-based therapy by bone marrow mononuclear cells (BMC) in a large-sized bone defect has already shown improved vascularization and new bone formation. First clinical trials are already being conducted. BMC were isolated from bone marrow aspirate and given back to patients in combination with a scaffold within some hours. However, the optimal concentration of BMC has not yet been determined for bone healing. With this study, we want to determine the optimal dosage of the BMC in the bone defect to support bone healing. Material and methods: Scaffolds with increasing BMC concentrations were inserted into a 5 mm femoral defect, cell concentrations of 2 × 106 BMC/mL, 1 × 107 BMC/mL and 2 × 107 BMC/mL were used. Based on the initial cell number used to colonize the scaffolds, the groups are designated 1 × 106, 5 × 106 and 1 × 107 group. Bone healing was assessed biomechanically, radiologically (µCT), and histologically after 8 weeks healing time. Results: Improved bone healing parameters were noted in the 1 × 106 and 5 × 106 BMC groups. A significantly higher BMD was observed in the 1 × 106 BMC group compared to the other groups. Histologically, a significantly increased bone growth in the defect area was observed in group 5 × 106 BMC. This finding could be supported radiologically. Conclusion: It was shown that the effective dose of BMC for bone defect healing ranges from 2 × 106 BMC/mL to 1 × 107 BMC/mL. This concentration range seems to be the therapeutic window for BMC-supported therapy of large bone defects. However, further studies are necessary to clarify the exact BMC-dose dependent mechanisms of bone defect healing and to determine the therapeutically effective range more precisely.

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Author:Maren Carina JankoORCiDGND, Sabrina Pöllinger, Alexander Schaible, Marlene BellenGND, Katrin SchröderORCiDGND, Myriam Widad HeilaniORCiDGND, Charlotte FremdlingGND, Ingo MarziORCiDGND, Christoph Heinrich Eugen NauGND, Dirk HenrichORCiDGND, René VerboketORCiDGND
Parent Title (English):European journal of trauma and emergency surgery
Publisher:Springer Medizin
Place of publication:Heidelberg
Document Type:Article
Date of Publication (online):2020/02/28
Date of first Publication:2020/02/28
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2023/01/13
Tag:BMC; Bone defect; Critical size; Tissue engineering
Page Number:12
First Page:265
Last Page:276
Open Access funding provided by Projekt DEAL.
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0