Biologization of collagen-based biomaterials using liquid-platelet-rich fibrin: New insights into clinically applicable tissue engineering
- Platelet-rich fibrin (PRF) is a blood concentrate derived from venous blood that is processed without anticoagulants by a one-step centrifugation process. This three-dimensional scaffold contains inflammatory cells and plasma proteins entrapped in a fibrin matrix. Liquid-PRF was developed based on the previously described low-speed centrifuge concept (LSCC), which allowed the introduction of a liquid-PRF formulation of fibrinogen and thrombin prior to its conversion to fibrin. Liquid-PRF was introduced to meet the clinical demand for combination with biomaterials in a clinically applicable and easy-to-use way. The aim of the present study was to evaluate, ex vivo, the interaction of the liquid-PRF constituents with five different collagen biomaterials by histological analyses. The results first demonstrated that large variability existed between the biomaterials investigated. Liquid-PRF was able to completely invade Mucograft® (MG; Geistlich Biomaterials, Wolhusen, Switzerland) and to partly invade Bio-Gide® (BG; Geistlich Biomaterials, Wolhusen, Switzerland) and Mucoderm® (MD; Botiss Biomaterials, Berlin, Germany), and Collprotect® (CP; Botiss Biomaterials, Berlin, Germany) showed only a superficial interaction. The BEGO® collagen membrane (BCM; BEGO Implant Systems) appeared to be completely free of liquid-PRF. These results were confirmed by the different cellular penetration and liquid-PRF absorption coefficient (PAC) values of the evaluated membranes. The present study demonstrates a system for loading biomaterials with a complex autologous cell system (liquid-PRF) in a relatively short period of time and in a clinically relevant manner. The combination of biomaterials with liquid-PRF may be clinically utilized to enhance the bioactivity of collagen-based biomaterials and may act as a biomaterial-based growth factor delivery system.
Verfasserangaben: | Sarah al- MaawiGND, Carlos Herrera-VizcaínoORCiDGND, Anna Orlowska, Ines Willershausen, Robert Alexander SaderORCiDGND, Richard J. Miron, Joseph Choukroun, Shahram Michael GhanaatiORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-527441 |
DOI: | https://doi.org/10.3390/ma12233993 |
ISSN: | 1996-1944 |
Pubmed-Id: | https://pubmed.ncbi.nlm.nih.gov/31810182 |
Titel des übergeordneten Werkes (Englisch): | Materials |
Verlag: | MDPI |
Verlagsort: | Basel |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Jahr der Fertigstellung: | 2019 |
Datum der Erstveröffentlichung: | 02.12.2019 |
Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Datum der Freischaltung: | 17.02.2020 |
Freies Schlagwort / Tag: | LSCC; centrifugation; collagen; leukocytes; liquid-PRF; platelet-rich fibrin; platelets; tissue engineering |
Jahrgang: | 12 |
Ausgabe / Heft: | 23, Art. 3993 |
Seitenzahl: | 17 |
Erste Seite: | 1 |
Letzte Seite: | 17 |
Bemerkung: | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
HeBIS-PPN: | 461423022 |
Institute: | Medizin / Medizin |
DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Open-Access-Publikationsfonds: | Medizin |
Lizenz (Deutsch): | Creative Commons - Namensnennung 4.0 |