Co-utilization of L-arabinose and D-xylose by laboratory and industrial Saccharomyces cerevisiae strains

  • Background Fermentation of lignocellulosic biomass is an attractive alternative for the production of bioethanol. Traditionally, the yeast Saccharomyces cerevisiae is used in industrial ethanol fermentations. However, S. cerevisiae is naturally not able to ferment the pentose sugars D-xylose and L-arabinose, which are present in high amounts in lignocellulosic raw materials. Results We describe the engineering of laboratory and industrial S. cerevisiae strains to co-ferment the pentose sugars D-xylose and L-arabinose. Introduction of a fungal xylose and a bacterial arabinose pathway resulted in strains able to grow on both pentose sugars. Introduction of a xylose pathway into an arabinose-fermenting laboratory strain resulted in nearly complete conversion of arabinose into arabitol due to the L-arabinose reductase activity of the xylose reductase. The industrial strain displayed lower arabitol yield and increased ethanol yield from xylose and arabinose. Conclusion Our work demonstrates simultaneous co-utilization of xylose and arabinose in recombinant strains of S. cerevisiae. In addition, the co-utilization of arabinose together with xylose significantly reduced formation of the by-product xylitol, which contributed to improved ethanol production.

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Metadaten
Author:Kaisa Karhumaa, Beate Wiedemann, Bärbel Hahn-Hägerdal, Eckhard Boles, Marie-Francoise Gorwa-Grauslund
URN:urn:nbn:de:hebis:30-27467
DOI:https://doi.org/10.1186/1475-2859-5-18
ISSN:1475-2859
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/16606456
Parent Title (English):Microbial cell factories
Publisher:BioMed Central
Place of publication:London
Document Type:Article
Language:English
Year of Completion:2006
Date of first Publication:2006/04/10
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2006/06/08
Volume:5
Issue:5:18
Note:
© 2006 Karhumaa et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
HeBIS-PPN:19149416X
Institutes:Biowissenschaften / Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Sammlung Biologie / Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung 2.0