Varicellovirus UL 49.5 proteins differentially affect the function of the transporter associated with antigen processing, TAP

  • Cytotoxic T-lymphocytes play an important role in the protection against viral infections, which they detect through the recognition of virus-derived peptides, presented in the context of MHC class I molecules at the surface of the infected cell. The transporter associated with antigen processing (TAP) plays an essential role in MHC class I–restricted antigen presentation, as TAP imports peptides into the ER, where peptide loading of MHC class I molecules takes place. In this study, the UL49.5 proteins of the varicelloviruses bovine herpesvirus 1 (BHV-1), pseudorabies virus (PRV), and equine herpesvirus 1 and 4 (EHV-1 and EHV-4) are characterized as members of a novel class of viral immune evasion proteins. These UL49.5 proteins interfere with MHC class I antigen presentation by blocking the supply of antigenic peptides through inhibition of TAP. BHV-1, PRV, and EHV-1 recombinant viruses lacking UL49.5 no longer interfere with peptide transport. Combined with the observation that the individually expressed UL49.5 proteins block TAP as well, these data indicate that UL49.5 is the viral factor that is both necessary and sufficient to abolish TAP function during productive infection by these viruses. The mechanisms through which the UL49.5 proteins of BHV-1, PRV, EHV-1, and EHV-4 block TAP exhibit surprising diversity. BHV-1 UL49.5 targets TAP for proteasomal degradation, whereas EHV-1 and EHV-4 UL49.5 interfere with the binding of ATP to TAP. In contrast, TAP stability and ATP recruitment are not affected by PRV UL49.5, although it has the capacity to arrest the peptide transporter in a translocation-incompetent state, a property shared with the BHV-1 and EHV-1 UL49.5. Taken together, these results classify the UL49.5 gene products of BHV-1, PRV, EHV-1, and EHV-4 as members of a novel family of viral immune evasion proteins, inhibiting TAP through a variety of mechanisms.

Download full text files

Export metadata

Metadaten
Author:Danijela Koppers-Lalic, Marieke C. VerweijORCiD, Andrea D. Lipinska, Ying Wang, Edwin Quinten, Eric A. Reits, Joachim KochGND, Sandra Loch, Marisa Marcondes Rezende, Franz Daus, Krystyna Bienkowska-Szewczyk, Nikolaus OsterriederORCiDGND, Thomas C. MettenleiterORCiDGND, Mirjam H. M. Heemskerk, Robert TampéORCiDGND, Jacques J. Neefjes, Shafiqul I. Chowdhury, Maaike E. Ressing, Frans A. M. Rijsewijk, Emmanuel J. H. J. WiertzORCiDGND
URN:urn:nbn:de:hebis:30-57824
DOI:https://doi.org/10.1371/journal.ppat.1000080
ISSN:1553-7374
Parent Title (English):PLoS pathogens
Publisher:PLoS
Place of publication:Lawrence, Kan.
Document Type:Article
Language:English
Date of Publication (online):2008/05/30
Date of first Publication:2008/05/30
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2008/09/25
Volume:4
Issue:(5): e1000080
Page Number:14
First Page:1
Last Page:14
Note:
© 2008 Koppers-Lalic et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
HeBIS-PPN:205901980
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Sammlung Biologie / Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung 3.0