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Institute
Die vorliegende Studie setzt sich mit dem Adjektiv "neu" und seinem slowakischen Äquivalent "nový" systembezogen und pragmatisch auseinander. Wir befassen uns kontrastiv mit der Bedeutungsstruktur, mit der Kollokabilität und lexikographischen Auffassung dieser Adjektive. Um alle erwähnten Ebenen in ihrer Komplexität zu erfassen, darf man sie nicht voneinander getrennt untersuchen. Bei unserer Untersuchung gehen wir von der kodifizierten Bedeutung aus, die wir mit der realen Sprachverwendung vergleichen und ihre Anwendbarkeit an der aus den Korpora gewonnenen Daten überprüfen. Bei unserer kontrastiven Vorhegensweise ist der Ausgangspunkt die Auslegung der slowakischen Bedeutungsbeschreibung der lexikalischen Einheit "nový". Zunächst erweitert sich der Forschungsgegenstand um den Vergleich der jeweiligen Erläuterungen der deutschen lexikalischen Einheit "neu" in verschiedenen deutschen Wörterbüchern. Im Anschluss an die semantische Analyse der einzelnen Adjektive in den zwei von uns ausgewählten Sprachen überprüfen wir, ob und inwiefern bei der Bedeutungsbeschreibung der Übersetzungsäquivalente "nový" und "neu" eine analogische Auslegung verwendbar ist.
Halting the loss of grassland biodiversity and restoring degraded ecosystems are high priority tasks in the EU Biodiversity Strategy. Sowing low-diversity seed mixtures is widely used in grassland restoration because of its high predictability and fast, promising results. Generally, the sown perennial grasses establish within a few years and form a dense sward, which effectively suppresses weeds. Unfortunately, these grasslands are often species-poor because the sown grasses hamper the colonisation of target grassland forbs. Our aim was to test a novel approach to increase the diversity of species-poor grasslands. We selected eight 8-year-old grasslands restored by low-diversity seed sowing where we created 32 establishment gaps by breaking up the grass sward and sowing a high-diversity seed mixture (35 native species). Altogether, we established three grazed gaps (1m × 1m, 2m × 2m and 4m × 4m) and one fenced gap (4m × 4m) per site and monitored the presence and abundance of sown and non-sown species within a time frame of two years. We asked the following questions: (1) Which target species establish most successfully? (2) What is the effect of establishment gap size on the establishment success of target species and weeds? (3) What is the effect of management (grazed versus not managed) on the species composition of the establishment gaps? Our results showed that by creating establishment gaps and sowing diverse seed mixtures, we were able to overcome microsite and propagule limitation, successfully introducing target species into the species-poor grasslands. We found that all sown species established in the gaps, and the majority of the species maintained or even increased their first-year cover in the second year. Smaller gaps were characterised by lower cover of sown species and a quite stochastic development compared to the larger ones. Weed cover was moderate in the first year and decreased significantly in the second year, regardless of gap size. Therefore, in restoration practice, the use of larger establishment gaps is recommended. We found that the cover of sown species and weeds were similar in the grazed and unmanaged gaps during our study. However, management by extensive grazing might be crucial in the long-term because livestock can disperse target species propagules and create microsites. Our study shows that establishment gaps can serve as biodiversity hotspots. Further studies need to clarify to what extent they can improve the restoration success across the entire grassland.
La lista preliminar de ácaros colectadas sobre musgos en México incluye 48 familias: 11 Mesostigmata, 17 Prostigmata (=Actinedida), 1 Astigmata y 19 Cryptostigmata (=Oribatei). Se presenta un análisis general de la relación entre las briofitas y su fauna asociada. El estudio detallado de estos grupos de animales seguramente dará a conocer un número mayor de especies asociadas con las briofitas.
Little is known about groundwater-dependent ecosystems in south-eastern Australia and few studies have examined the impact of landclearing upslope of such ecosystems. The eastern edge of Porters Creek wetland, near Warnervale on the Central Coast of New South Wales, supports a Eucalyptus parramattensis subsp. Parramattensis (Parramatta Red Gum) population. The population has been listed as an Endangered Population (EP) and was showing signs of dieback during the 2002 to mid-2007 drought. Water table depth, soil moisture tension, soil temperature and groundwater salinity beneath this woodland were measured to investigate whether the woodland is associated with a shallow aquifer and/or high soil moisture conditions, and whether an upslope construction development would impact on the ecosystem. The composition, structure and health of the Eucalyptus parramattensis Woodland was also examined. Monitoring over 5 years demonstrated that Eucalyptus parramattensis is associated with a shallow, slightly saline to saline (8–12 ppt) water table, 1.4–3.1 m beneath the surface of a drainage depression wetland. During the drought water table drawdown rates were quite rapid, and rainfall events took up to a month to replenish water table levels. Landclearing for an upslope development significantly decreased the average water table level by 0.3–0.5 m during the construction phase, while peaks that occurred at the base of the slope after large rainfall events reversed the normal direction of the water table gradient. The water table re-stabilised to pre-construction levels after construction was completed and the area was landscaped and revegetated with grass. The short-term alteration of groundwater conditions did not appear to impact on tree health; the poor tree health observed prior to construction was most likely due to the lowering of the water table (and/or an increase in groundwater/soil salinity) during the drought years. Our results suggest that developments that revegetate slopes above wetlands as quickly as possible after clearing, orundertake clearing in stages, will minimise effects on water table levels. Pervious detention basins designed to capture runoff and allow infiltration into the soil help replenish aquifers, but also require weed maintenance. This study highlights the significance of maintaining shallow aquifer and catchment processes for the conservation and rehabilitation of coastal wetlands, many of which are listed as Endangered Populations or Endangered Ecological Communities.
The Intensifying Function of Modal Particles and Modal Elements in a Cross-Linguistic Perspective
(2015)
The aim of this paper is to analyze the intensifying function of German modal particles and equivalent modal expressions in Croatian and English. Our hypothesis is that some modal particles in German and their functional equivalents in Croatian and English can express different degrees of intensity and types of intensification. The presented study comprises two parts. First, the use of intensifying modal particles by a group of speakers of L1 Croatian and L2 German/English is investigated. On the basis of the results obtained, and by means of a previously conducted corpus analysis (cf. Kresić and Batinić 2014), an intensification scale with respect to the inventory of German modal particles and corresponding particles in Croatian as well as equivalent English expressions is suggested. Some German and Croatian modal particles and equivalent modal elements in English can be classified on the upper and partially on the lower part of the proposed intensification scale when compared to the norm, i.e. an utterance unmarked by a modal particle.
Nakon uvodnih napomena o gornjomeđimurskome naselju Dragoslavec Breg (s osobitim osvrtom na povijest njegova imena, neslužbeni etnik i kretanje broja stanovnika), u radu se na osnovi terenskim i arhivskim istraživanjem prikupljene građe analizira antroponimijski sustav toga naselja. Pokazuju se značajke osobnih imena (njihova službena i neslužbena uporaba te učestalost), prezimena njegovih stanovnika (učestalost, podrijetlo, značenje i tvorbena struktura) kao i njihovi obiteljski nadimci (motivacija i tvorbene značajke).
The successful management and restoration of riparian corridors in Australia is currently hindered by our poor understanding of the links between hydrology, fluvial geomorphology and plant population dynamics. The aim of the current study was to determine the survival rates of Casuarina cunninghamiana subsp. cunninghamiana Miq. (family Casuarinaceae) on a sand-bed stream in the Wollombi Valley, a tributary of the Hunter River system, in coastal New South Wales (lat 33°02’S; long 151°10’E). A population planted on the banks of Dairy Arm in 1987, as part of river training works, was used to determine the long-term (24-year) survival rate. A bankfull flood in June 2011 provided an opportunity to examine the survival response of seedlings regenerating naturally within the channel. We found that 24% of the seedlings planted on the banks in 1987 had survived to adulthood. The bankfull flood significantly decreased (d.f. = 14; W= - 30; p = 0.0024) median seedling density within the channel from 12 to 2 individuals per 100 m2. Seedling survival varied with height, with seedlings > 15 cm more likely to survive the bankfull flood. The percentage of seedlings partially buried by sediment was significantly higher (d.f. = 14; W = 13; p = 0.016) after flood compared to before flood. Seedling density was positively correlated with the amount of bare ground prior to the flood (r = 0.61; p = 0.02), but this relationship was no longer significant after flood (r = 0.18; p = 0.53). 37% of the seedlings surveyed showed evidence of grazing. Our results confirm that hydrogeomorphic processes associated with a bankfull flood affect the survival of Casuarina cunninghamiana seedlings. The management implications of our findings are discussed in terms of riparian revegetation techniques and the geomorphic recovery of over-widened sand-bed streams.
Diploid transgenic organisms are either hemi- or homozygous. Genetic assays are, therefore, required to identify the genotype. Our AGameOfClones vector concept uses two clearly distinguishable transformation markers embedded in interweaved, but incompatible Lox site pairs. Cre-mediated recombination leads to hemizygous individuals that carry only one marker. In the following generation, heterozygous descendants are identified by the presence of both markers and produce homozygous progeny that are selected by the lack of one marker. We prove our concept in Tribolium castaneum by systematically creating multiple functional homozygous transgenic lines suitable for long-term fluorescence live imaging. Our approach saves resources and simplifies transgenic organism handling. Since the concept relies on the universal Cre-Lox system, it is expected to work in all diploid model organisms, for example, insects, zebrafish, rodents and plants. With appropriate adaptions, it can be used in knock-out assays to preselect homozygous individuals and thus minimize the number of wasted animals.
Supersaturating formulations are widely used to improve the oral bioavailability of poorly soluble drugs. However, supersaturated solutions are thermodynamically unstable and such formulations often must include a precipitation inhibitor (PI) to sustain the increased concentrations to ensure that sufficient absorption will take place from the gastrointestinal tract. Recent advances in understanding the importance of drug-polymer interaction for successful precipitation inhibition have been encouraging. However, there still exists a gap in how this newfound understanding can be applied to improve the efficiency of PI screening and selection, which is still largely carried out with trial and error-based approaches. The aim of this study was to demonstrate how drug-polymer mixing enthalpy, calculated with the Conductor like Screening Model for Real Solvents (COSMO-RS), can be used as a parameter to select the most efficient precipitation inhibitors, and thus realise the most successful supersaturating formulations. This approach was tested for three different Biopharmaceutical Classification System (BCS) II compounds: dipyridamole, fenofibrate and glibenclamide, formulated with the supersaturating formulation, mesoporous silica. For all three compounds, precipitation was evident in mesoporous silica formulations without a precipitation inhibitor. Of the nine precipitation inhibitors studied, there was a strong positive correlation between the drug-polymer mixing enthalpy and the overall formulation performance, as measured by the area under the concentration-time curve in in vitro dissolution experiments. The data suggest that a rank-order based approach using calculated drug-polymer mixing enthalpy can be reliably used to select precipitation inhibitors for a more focused screening. Such an approach improves efficiency of precipitation inhibitor selection, whilst also improving the likelihood that the most optimal formulation will be realised.
Objectives Supersaturating formulations hold great promise for delivery of poorly soluble active pharmaceutical ingredients (APIs). To profit from supersaturating formulations, precipitation is hindered with precipitation inhibitors (PIs), maintaining drug concentrations for as long as possible. This review provides a brief overview of supersaturation and precipitation, focusing on precipitation inhibition. Trial-and-error PI selection will be examined alongside established PI screening techniques. Primarily, however, this review will focus on recent advances that utilise advanced analytical techniques to increase mechanistic understanding of PI action and systematic PI selection.
Key Findings. Advances in mechanistic understanding have been made possible by the use of analytical tools such as spectroscopy, microscopy and mathematical and molecular modelling, which have been reviewed herein. Using these techniques, PI selection can instead be guided by molecular rationale. However, more work is required to see wide-spread application of such an approach for PI selection.
Conclusions PIs are becoming increasingly important in enabling formulations. Trial-and-error approaches have seen success thus far. However, it is essential to learn more about the mode of action of PIs if the most optimal formulations are to be realised. Robust analytical tools, and the knowledge of where and how they can be applied, will be essential in this endeavour.
In New South Wales, the Threatened Species Conservation Act 1995 makes provision for the listing of Threatened Ecological Communities (assemblages of species occupying a particular area) as a means to conserving the variety of ecosystems present in the State. Hunter Valley Weeping Myall Woodland is listed as an Endangered Ecological Community under the New South Wales Threatened Species Conservation (TSC) Act 1995 and as a Critically Endangered Ecological Community under the Commonwealth Environment Protection and Biodiversity Conservation (EPBC) Act 1999.
The provenance of populations of Weeping Myall (Acacia pendula, family Fabaceae) in the Hunter Valley has recently been questioned. In this paper we consider the implications of its hypothesized European introduction for listing Hunter Valley Weeping Myall Woodland under the TSC and EPBS Acts. The recognition of any community is contingent on the identification of both a particular assemblage of species and the critical environmental factors that determine their co-occurrence (the area the community occupies in environmental space). Acacia pendula is one of a range of semiarid species which are primarily distributed west of the Great Dividing Range but also occur on Permian sediments in areas receiving the lowest rainfall of the Hunter Valley floor. On this basis we argue there is sufficient evidence to support the listing of Hunter Valley Weeping Myall Woodland under both Acts.
Background: Chronic hepatitis C virus (HCV) infections are causally linked with metabolic comorbidities such as insulin resistance, hepatic steatosis, and dyslipidemia. However, the clinical impact of HCV eradication achieved by direct-acting antivirals (DAAs) on glucose and lipid homeostasis is still controversial. The study aimed to prospectively investigate whether antiviral therapy of HCV with DAAs alters glucose and lipid parameters. Methods: 50 patients with chronic HCV who were treated with DAAs were screened, and 49 were enrolled in the study. Biochemical and virological data, as well as noninvasive liver fibrosis parameters, were prospectively collected at baseline, at the end of treatment (EOT) and 12 and 24 weeks post-treatment. Results: 45 of 46 patients achieved sustained virologic response (SVR). The prevalence of insulin resistance (HOMA-IR) after HCV clearance was significantly lower, compared to baseline (5.3 ± 6.1 to 2.5 ± 1.9, p < 0.001), which is primarily attributable to a significant decrease of fasting insulin levels (18.9 ± 17.3 to 11.7 ± 8.7; p = 0.002). In contrast to that, HCV eradication resulted in a significant increase in cholesterol levels (total cholesterol, low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein (HDL-C) levels) and Controlled Attenuated Score (CAP), although BMI did not significantly change over time (p = 0.95). Moreover, HOMA-IR correlated significantly with noninvasive liver fibrosis measurements at baseline und during follow-up (TE: r = 0.45; p = 0.003, pSWE: r = 0.35; p = 0.02, APRI: r = 0.44; p = 0.003, FIB-4: r = 0.41; p < 0.001). Conclusion: Viral eradication following DAA therapy may have beneficial effects on glucose homeostasis, whereas lipid profile seems to be worsened.
Wolves (Canis lupus) are currently showing a remarkable comeback in the highly frag-mented cultural landscapes of Germany. We here show that wolf numbers increasedexponentially between 2000 and 2015 with an annual increase of about 36%. Wedemonstrate that the first territories in each newly colonized region were establishedover long distances from the nearest known reproducing pack on active militarytraining areas (MTAs). We show that MTAs, rather than protected areas, served asstepping-stones for the recolonization of Germany facilitating subsequent spreadingof wolf territories in the surrounding landscape. We did not find any significant differ-ence between MTAs and protected areas with regard to habitat. One possible reasonfor the importance of MTAs may be their lower anthropogenic mortality rates com-pared to protected and other areas. To our knowledge, this is the first documented casewhere MTAs facilitate the recolonization of an endangered species across large areas.
The haloarchaeon Haloferax volcanii was shown to contain 145 intergenic and 45 antisense sRNAs. In a comprehensive approach to unravel various biological roles of haloarchaeal sRNAs in vivo, 27 sRNA genes were selected and deletion mutants were generated. The phenotypes of these mutants were compared to that of the parent strain under ten different conditions, i.e. growth on four different carbon sources, growth at three different salt concentrations, and application of four different stress conditions. In addition, cell morphologies in exponential and stationary phase were observed. Furthermore, swarming of 17 mutants was analyzed. 24 of the 27 mutants exhibited a difference from the parent strain under at least one condition, revealing that haloarchaeal sRNAs are involved in metabolic regulation, growth under extreme conditions, regulation of morphology and behavior, and stress adaptation. Notably, 7 deletion mutants showed a gain of function phenotype, which has not yet been described for any other prokaryotic sRNA gene deletion mutant. Comparison of the transcriptomes of one sRNA gene deletion mutant and the parent strain led to the identification of differentially expressed genes. Genes for flagellins and chemotaxis were up-regulated in the mutant, in accordance with its gain of function swarming phenotype. While the deletion mutant analysis underscored that haloarchaeal sRNAs are involved in many biological functions, the degree of conservation is extremely low. Only 3 of the 27 genes are conserved in more than 10 haloarchaeal species. 22 of the 27 genes are confined to H. volcanii, indicating a fast evolution of haloarchaeal sRNA genes.
Shikonin reduces growth of docetaxel-resistant prostate cancer cells mainly through necroptosis
(2021)
Simple Summary: Prostate carcinoma (PCa) is the most common tumor in men with an increasing age-associated risk. Several therapy strategies, one of which is docetaxel (DX) chemotherapy, have been established. However, due to the development of therapy resistance, in which chemotherapy no longer effectively combats the cancer, advanced, metastasized PCa with a poor prognosis may become manifested and therapy inevitably fails. Thus, new treatment options are urgently needed. Shikonin (SHI), from Traditional Chinese Medicine, has revealed promising antitumor activity in several tumor entities. In the current study, the impact of SHI on four therapy-sensitive and four respective DX-resistant PCa cell lines was determined. SHI induced growth inhibition mainly by necroptosis, a type of cell death, in all the tested therapy-sensitive, but more importantly, DX-resistant PCa cell lines. Corresponding molecular alterations contributing to growth inhibition after SHI exposure were found. SHI could, therefore, be a promising additive in treating advanced PCa.
Abstract: The prognosis for advanced prostate carcinoma (PCa) remains poor due to development of therapy resistance, and new treatment options are needed. Shikonin (SHI) from Traditional Chinese Medicine has induced antitumor effects in diverse tumor entities, but data related to PCa are scarce. Therefore, the parental (=sensitive) and docetaxel (DX)-resistant PCa cell lines, PC3, DU145, LNCaP, and 22Rv1 were exposed to SHI [0.1–1.5 μM], and tumor cell growth, proliferation, cell cycling, cell death (apoptosis, necrosis, and necroptosis), and metabolic activity were evaluated. Correspondingly, the expression of regulating proteins was assessed. Exposure to SHI time- and dose-dependently inhibited tumor cell growth and proliferation in parental and DX-resistant PCa cells, accompanied by cell cycle arrest in the G2/M or S phase and modulation of cell cycle regulating proteins. SHI induced apoptosis and more dominantly necroptosis in both parental and DX-resistant PCa cells. This was shown by enhanced pRIP1 and pRIP3 expression and returned growth if applying the necroptosis inhibitor necrostatin-1. No SHI-induced alteration in metabolic activity of the PCa cells was detected. The significant antitumor effects induced by SHI to parental and DX-resistant PCa cells make the addition of SHI to standard therapy a promising treatment strategy for patients with advanced PCa.
Members of the Sm protein family are important for the cellular RNA metabolism in all three domains of life. The family includes archaeal and eukaryotic Lsm proteins, eukaryotic Sm proteins and archaeal and bacterial Hfq proteins. While several studies concerning the bacterial and eukaryotic family members have been published, little is known about the archaeal Lsm proteins. Although structures for several archaeal Lsm proteins have been solved already more than ten years ago, we still do not know much about their biological function, however one can confidently propose that the archaeal Lsm proteins will also be involved in RNA metabolism. Therefore, we investigated this protein in the halophilic archaeon Haloferax volcanii. The Haloferax genome encodes a single Lsm protein, the lsm gene overlaps and is co-transcribed with the gene for the ribosomal L37.eR protein. Here, we show that the reading frame of the lsm gene contains a promoter which regulates expression of the overlapping rpl37R gene. This rpl37R specific promoter ensures high expression of the rpl37R gene in exponential growth phase. To investigate the biological function of the Lsm protein we generated a lsm deletion mutant that had the coding sequence for the Sm1 motif removed but still contained the internal promoter for the downstream rpl37R gene. The transcriptome of this deletion mutant was compared to the wild type transcriptome, revealing that several genes are down-regulated and many genes are up-regulated in the deletion strain. Northern blot analyses confirmed down-regulation of two genes. In addition, the deletion strain showed a gain of function in swarming, in congruence with the up-regulation of transcripts encoding proteins required for motility.
The recent recolonisation of the Central European lowland (CEL) by the grey wolf (Canis lupus) provides an excellent opportunity to study the effect of founder events on endoparasite diversity. Which role do prey and predator populations play in the re-establishment of endoparasite life cycles? Which intrinsic and extrinsic factors control individual endoparasite diversity in an expanding host population? In 53 individually known CEL wolves sampled in Germany, we revealed a community of four cestode, eight nematode, one trematode and 12 potential Sarcocystis species through molecular genetic techniques. Infections with zoonotic Echinococcus multilocularis, Trichinella britovi and T. spiralis occurred as single cases. Per capita endoparasite species richness and diversity significantly increased with population size and changed with age, whereas sex, microsatellite heterozygosity, and geographic origin had no effect. Tapeworm abundance (Taenia spp.) was significantly higher in immigrants than natives. Metacestode prevalence was slightly higher in ungulates from wolf territories than from control areas elsewhere. Even though alternative canid definitive hosts might also play a role within the investigated parasite life cycles, our findings indicate that (1) immigrated wolves increase parasite diversity in German packs, and (2) prevalence of wolf-associated parasites had declined during wolf absence and has now risen during recolonisation.