Refine
Author
- Das, Saumya (3)
- Balkom, Bastiaan Wilhelmus Maria van (2)
- Buck, Amy H. (2)
- Buzás, Edit I. (2)
- Driedonks, Tom A. P. (2)
- Hill, Andrew F. (2)
- Jones, Jennifer C. (2)
- Lorenowicz, Magdalena J. (2)
- Lunavat, Taral Rameshchand (2)
- Lässer, Cecilia (2)
- Maas, Sybren L. N. (2)
- Momma, Stefan (2)
- Nolte-’t Hoen, Esther N. M. (2)
- Pegtel, Dirk Michiel (2)
- Schiffelers, Raymond M. (2)
- Tahara, Hidetoshi (2)
- Théry, Clotilde (2)
- Tosar, Juan Pablo (2)
- Van Keuren-Jensen, Kendall R. (2)
- Wauben, Marca H. M. (2)
- Witwer, Kenneth W. (2)
- de-Candia, Paola (2)
- Aikawa, Elena (1)
- Alcaraz, Maria Jose (1)
- Anderson, Johnathon D. (1)
- Andriantsitohaina, Ramaroson (1)
- Antoniou, Anna (1)
- Arab, Tanina (1)
- Archer, Fabienne (1)
- Atkin-Smith, Georgia K. (1)
- Ayre, D. Craig (1)
- Bach, Jean-Marie (1)
- Bachurski, Daniel (1)
- Baharvand, Hossein (1)
- Balaj, Leonora (1)
- Baldacchino, Shawn (1)
- Bartel, Sabine (1)
- Bauer, Natalie N. (1)
- Baxter, Amy A. (1)
- Bebawy, Mary (1)
- Beckham, Carla (1)
- Benmoussa, Abderrahim (1)
- Berardi, Anna C. (1)
- Bergese, Paolo (1)
- Bhattacharyya, Suvendra N. (1)
- Bielska, Ewa (1)
- Blenkiron, Cherie (1)
- Bobis-Wozowicz, Sylwia (1)
- Boilard, Eric (1)
- Boireau, Wilfrid (1)
- Bongiovanni, Antonella (1)
- Borràs, Francesc E. (1)
- Bosch, Steffi (1)
- Boulanger, Chantal M. (1)
- Breakefield, Xandra (1)
- Breglio, Andrew M. (1)
- Brennan, Meadhbh Aín (1)
- Brigstock, David R. (1)
- Brisson, Alain (1)
- Broekman, Marike L. D. (1)
- Bromberg, Jacqueline F. (1)
- Bryl-Gorecka, Paulina (1)
- Buch, Shilpa (1)
- Burger, Dylan (1)
- Busatto, Sara (1)
- Buschmann, Dominik (1)
- Bussolati, Benedetta (1)
- Byrd, James Bryan (1)
- Camussi, Giovanni (1)
- Carter, David R. F. (1)
- Caruso, Sarah (1)
- Chamley, Lawrence W. (1)
- Chang, Yu-Ting (1)
- Chaudhuri, Amrita Datta (1)
- Chen, Chihchen (1)
- Chen, Shuai (1)
- Cheng, Lesley (1)
- Chin, Andrew R. (1)
- Chow, Franklin W. N. (1)
- Clayton, Aled (1)
- Clerici, Stefano P. (1)
- Cocks, Alex (1)
- Cocucci, Emanuele (1)
- Coffey, Robert J. (1)
- Cordeiro-da-Silva, Anabela (1)
- Couch, Yvonne (1)
- Coumans, Frank A. W. (1)
- Coyle, Beth (1)
- Crescitelli, Rossella (1)
- Criado, Miria Ferreira (1)
- Das, Avash (1)
- De Santana, Eliezer F. (1)
- De Wever, Olivier (1)
- Deller, Thomas (1)
- Demaret, Tanguy (1)
- Deville, Sarah (1)
- Devitt, Andrew (1)
- Dhondt, Bert (1)
- Di Liegro, Italia (1)
- Di Vizio, Dolores (1)
- Dieterich, Lothar C. (1)
- Dolo, Vincenza (1)
- Dominguez Rubio, Ana Paula (1)
- Dominici, Massimo (1)
- Dourado, Mauricio R. (1)
- Duarte, Filipe V. (1)
- Duncan, Heather M. (1)
- D’Souza-Schorey, Crislyn (1)
- Eichenberger, Ramon Marc (1)
- Ekström, Karin (1)
- El Andaloussi, Samir (1)
- Elie-Caille, Celine (1)
- Erdbrügger, Uta (1)
- Falcón-Pérez, Juan M. (1)
- Fan, Wei (1)
- Fatima, Farah (1)
- Fernández-Messina, Lola (1)
- Fish, Jason E. (1)
- Flores-Bellver, Miguel (1)
- Frelet-Barrand, Annie (1)
- Fricke, Fabia (1)
- Fu, Ting (1)
- Fuhrmann, Gregor (1)
- Försönits, András (1)
- Gabrielsson, Susanne (1)
- Gardiner, Chris (1)
- Gaudin, Raphael (1)
- Gho, Yong Song (1)
- Giebel, Bernd (1)
- Gilbert, Caroline (1)
- Gimona, Mario (1)
- Giusti, Ilaria (1)
- Goberdhan, Deborah C. I. (1)
- Gorski, Sharon M. (1)
- Greening, David W. (1)
- Grein, Susanne G. van der (1)
- Groß, Julia Christina (1)
- Gualerzi, Alice (1)
- Gupta, Gopal N. (1)
- Gustafson, Dakota (1)
- Gámez-Valero, Ana (1)
- Gärtner, Kathrin (1)
- Görgens, André (1)
- Haderk, Franziska (1)
- Handberg, Aase (1)
- Haraszti, Réka Ágnes (1)
- Harrison, Paul (1)
- Hegyesi, Hargita (1)
- Hendrix, An (1)
- Herwijnen, Martijn J. C. van (1)
- Hochberg, Fred H. (1)
- Hoffmann, Karl F. (1)
- Holder, Beth (1)
- Holthofer, Harry (1)
- Hooten, Nicole Noren (1)
- Hosseinkhani, Baharak (1)
- Hu, Guoku (1)
- Huang, Yiyao (1)
- Huber, Veronica (1)
- Hunt, Stuart (1)
- Ibrahim, Ahmed Gamal-Eldin (1)
- Ikezu, Tsuneya (1)
- Inal, Jameel M. (1)
- Isin, Mustafa (1)
- Ivanova, Alena (1)
- Jackson, Hannah K. (1)
- Jacobsen, Soren (1)
- Jay, Steven M. (1)
- Jayachandran, Muthuvel (1)
- Jenster, Guido (1)
- Jiang, Lanzhou (1)
- Johnson, Suzanne M. (1)
- Jong, Ambrose (1)
- Jovanovic-Talisman, Tijana (1)
- Jung, Stephanie (1)
- Kalluri, Raghu (1)
- Kano, Shin-ichi (1)
- Kaur, Sukhbir (1)
- Kawamura, Yumi (1)
- Keller, Evan T. (1)
- Khamari, Delaram (1)
- Khomyakova, Elena (1)
- Khvorova, Anastasia (1)
- Kierulf, Peter (1)
- Kim, Kwang Pyo (1)
- Kislinger, Thomas (1)
- Klingeborn, Mikael (1)
- Klinke, David J. (1)
- Kornek, Miroslaw (1)
- Kosanović, Maja M. (1)
- Kovács, Árpád Ferenc (1)
- Kowal, Emma (1)
- Krasemann, Susanne (1)
- Krause, Mirja (1)
- Krämer-Albers, Eva-Maria (1)
- Kur, Ivan-Maximiliano (1)
- Kurochkin, Igor V. (1)
- Kusuma, Gina D. (1)
- Kuypers, Sören (1)
- Lai, Charles P. (1)
- Laitinen, Saara (1)
- Langevin, Scott M. (1)
- Languino, Lucia R. (1)
- Lannigan, Joanne (1)
- Laurent, Louise C. (1)
- Lavieu, Gregory (1)
- Le Lay, Soazig (1)
- Lee, Myung-Shin (1)
- Lee, Yi Xin Fiona (1)
- Lemos, Debora S. (1)
- Lenassi, Metka (1)
- Leszczynska, Aleksandra (1)
- Li, Isaac T. S. (1)
- Liao, Ke (1)
- Libregts, Sten F. (1)
- Ligeti, Erzsebet (1)
- Lim, Rebecca (1)
- Lim, Sai Kiang (1)
- Linnemannstöns, Karen (1)
- Linē, Aija (1)
- Llorente, Alicia (1)
- Lombard, Catherine Ann (1)
- Lovett, Jason (1)
- Lowry, Michelle C. (1)
- Loyer, Xavier (1)
- Lu, Quan (1)
- Lukomska, Barbara (1)
- Lázaro-Ibáñez, Elisa (1)
- Lörincz, Ákos M. (1)
- Lötvall, Jan (1)
- Malhi, Harmeet (1)
- Marcilla, Antonio (1)
- Mariani, Jacopo (1)
- Mariscal, Javier (1)
- Martens-Uzunova, Elena S. (1)
- Martin-Jaular, Lorena (1)
- Martinez, M. Carmen (1)
- Martins, Vilma Regina (1)
- Mateescu, Bogdan (1)
- Mathieu, Mathilde (1)
- Mathivanan, Suresh (1)
- Maugeri, Marco (1)
- McGinnis, Lynda K. (1)
- McVey, Mark J. (1)
- Meckes, David G. (1)
- Meehan, Katie (1)
- Mertens, Inge (1)
- Minciacchi, Valentina R. (1)
- Mittelbrunn, Maria (1)
- Morales-Kastresana, Aizea (1)
- Morhayim, Jess (1)
- Mukherjee, Kamalika (1)
- Mullier, François (1)
- Muraca, Maurizio (1)
- Musante, Luca (1)
- Mussack, Veronika (1)
- Muth, Dillon C. (1)
- Myburgh, Kathryn H. (1)
- Mäger, Imre (1)
- Möller, Andreas (1)
- Møller Jørgensen, Malene (1)
- Müller-Braun, Felicia (1)
- Najrana, Tanbir (1)
- Nawaz, Muhammad (1)
- Nawaz, Muhammed (1)
- Nazarenko, Irina (1)
- Nejsum, Peter (1)
- Neri, Christian (1)
- Neri, Tommaso (1)
- Niel, Guillaume van (1)
- Nieuwland, Rienk (1)
- Nimrichter, Leonardo (1)
- Nolan, John P. (1)
- Ochiya, Takahiro (1)
- Olivier, Martin (1)
- Ortiz, Alberto (1)
- Ortiz, Luis A. (1)
- Osteikoetxea, Xabier (1)
- Ostrowski, Matias (1)
- O’Driscoll, Lorraine (1)
- O’Grady, Tina (1)
- O’Loghlen, Ana (1)
- Park, Jaesung (1)
- Peinado, Hector (1)
- Perut, Francesca (1)
- Pfaff, Michael W. (1)
- Pfaffl, Michael (1)
- Phinney, Donald G. (1)
- Pieters, Bartijn C. H. (1)
- Pink, Ryan Charles (1)
- Pisetsky, David S. (1)
- Pogge von Strandmann, Elke (1)
- Polakovicova, Iva (1)
- Poon, Ivan K. H. (1)
- Powell, Bonita H. (1)
- Prada, Ilaria (1)
- Prouvot, Pierre-Hugues (1)
- Pulliam, Lynn (1)
- Quesenberry, Peter (1)
- Radeghieri, Annalisa (1)
- Raffai, Robert L. (1)
- Raimondo, Stefania (1)
- Rak, Janusz (1)
- Ramirez, Marcel I. (1)
- Raposo, Graça (1)
- Rayyan, Morsi S. (1)
- Regev-Rudzki, Neta (1)
- Ricklefs, Franz Lennard (1)
- Robbins, Paul D. (1)
- Roberts, David D. (1)
- Rodrigues, Silvia C. (1)
- Roeper, Jochen (1)
- Rohde, Eva (1)
- Rome, Sophie (1)
- Rouschop, Kasper M. (1)
- Royen, Martin E. van (1)
- Rughetti, Aurelia (1)
- Russell, Ashley E. (1)
- Sahoo, Susmita (1)
- Salas-Huenuleo, Edison (1)
- Saugstad, Julie A. (1)
- Saul, Meike J. (1)
- Saá, Paula (1)
- Schneider, Raphael (1)
- Schøyen, Tine Hiorth (1)
- Scott, Aaron (1)
- Shahaj, Eriomina (1)
- Sharma, Shivani (1)
- Shatnyeva, Olga (1)
- Shekari, Faezeh (1)
- Shelke, Ganesh Vilas (1)
- Shetty, Ashok K. (1)
- Shiba, Kiyotaka (1)
- Siljander, Pia R.-M. (1)
- Silva, Andreia M. (1)
- Skowronek, Agata (1)
- Snyder, Orman L. (1)
- Soares, Rodrigo Pedro (1)
- Soekmadji, Carolina (1)
- Sotillo, Javier (1)
- Stahl, Philip D. (1)
- Stoorvogel, Willem (1)
- Stott, Shannon L. (1)
- Strasser, Erwin F. (1)
- Stroh, Albrecht (1)
- Swift, Simon (1)
- Sánchez, Catherine (1)
- Sódar, Barbara W. (1)
- Tewari, Muneesh (1)
- Timms, Kate (1)
- Tiwari, Swasti (1)
- Tixeira, Rochelle (1)
- Tkach, Mercedes (1)
- Toh, Wei Seong (1)
- Tomasini, Richard (1)
- Torrecilhas, Ana Claudia (1)
- Toxavidis, Vasilis (1)
- Urbanelli, Lorena (1)
- Vader, Pieter (1)
- Van Deun, Jan (1)
- Vasconcelos, M. Helena (1)
- Vechetti, Ivan J. (1)
- Veit, Tiago D. (1)
- Vella, Laura J. (1)
- Velot, Émilie (1)
- Verweij, Frederik (1)
- Vestad, Beate (1)
- Visnovitz, Tamás (1)
- Viñas, Jose Luis (1)
- Vukman, Krisztina V. (1)
- Wahlgren, Jessica (1)
- Watson, Dionysios C. (1)
- Weaver, Alissa (1)
- Webber, Jason P. (1)
- Weber, Viktoria (1)
- Wehman, Ann M. (1)
- Weiss, Daniel J. (1)
- Welsh, Joshua A. (1)
- Wendt, Sebastian (1)
- Wheelock, Åsa M. (1)
- Wiener, Zoltán (1)
- Wijnen, Andre J. van (1)
- Witte, Leonie (1)
- Wolfram, Joy (1)
- Xagorari, Angeliki (1)
- Xander, Patricia (1)
- Xu, Jing (1)
- Yan, Xiaomei (1)
- Yin, Hang (1)
- Yuana, Yuana (1)
- Yáñez-Mó, María (1)
- Zappulli, Valentina (1)
- Zarubova, Jana (1)
- Zavec, Apolonija Bedina (1)
- Zhang, Jian-ye (1)
- Zhao, Zezhou (1)
- Zheng, Lei (1)
- Zheutlin, Alexander R. (1)
- Zickler, Antje Maria (1)
- Zimmermann, Pascale (1)
- Zivkovic, Angela M. (1)
- Zocco, Davide (1)
- Zuba-Surma, Ewa K. (1)
- del Portillo, Hernando A. (1)
- Østergaard, Ole (1)
- Žėkas, Vytautas (1)
Document Type
- Article (3)
Language
- English (3)
Has Fulltext
- yes (3)
Is part of the Bibliography
- no (3)
Keywords
- exosomes (2)
- Extracellular vesicles (1)
- Gene expression (1)
- Intravenous injections (1)
- Marker genes (1)
- Microglial cells (1)
- Mouse models (1)
- Neurons (1)
- Optogenetics (1)
- RNA binding proteins (1)
Institute
- Biowissenschaften (2)
- Medizin (2)
- Georg-Speyer-Haus (1)
Kur, Ivan-Maximiliano ; Prouvot, Pierre-Hugues ; Fu, Ting ; Fan, Wei ; Müller-Braun, Felicia ; Das, Avash ; Das, Saumya ; Deller, Thomas ; Roeper, Jochen ; Stroh, Albrecht ; Momma, Stefan
Communication with the hematopoietic system is a vital component of regulating brain function in health and disease. Traditionally, the major routes considered for this neuroimmune communication are by individual molecules such as cytokines carried by blood, by neural transmission, or, in more severe pathologies, by the entry of peripheral immune cells into the brain. In addition, functional mRNA from peripheral blood can be directly transferred to neurons via extracellular vesicles (EVs), but the parameters that determine their uptake are unknown. Using varied animal models that stimulate neuronal activity by peripheral inflammation, optogenetics, and selective proteasome inhibition of dopaminergic (DA) neurons, we show that the transfer of EVs from blood is triggered by neuronal activity in vivo. Importantly, this transfer occurs not only in pathological stimulation but also by neuronal activation caused by the physiological stimulus of novel object placement. This discovery suggests a continuous role of EVs under pathological conditions as well as during routine cognitive tasks in the healthy brain.
Mateescu, Bogdan ; Kowal, Emma ; Balkom, Bastiaan Wilhelmus Maria van ; Bartel, Sabine ; Bhattacharyya, Suvendra N. ; Buzás, Edit I. ; Buck, Amy H. ; de-Candia, Paola ; Chow, Franklin W. N. ; Das, Saumya ; Driedonks, Tom A. P. ; Fernández-Messina, Lola ; Haderk, Franziska ; Hill, Andrew F. ; Jones, Jennifer C. ; Van Keuren-Jensen, Kendall R. ; Lai, Charles P. ; Lässer, Cecilia ; Di Liegro, Italia ; Lunavat, Taral Rameshchand ; Lorenowicz, Magdalena J. ; Maas, Sybren L. N. ; Mäger, Imre ; Mittelbrunn, Maria ; Momma, Stefan ; Mukherjee, Kamalika ; Mukherjee, Kamalika ; Nawaz, Muhammed ; Pegtel, Dirk Michiel ; Pfaff, Michael W. ; Schiffelers, Raymond M. ; Tahara, Hidetoshi ; Théry, Clotilde ; Tosar, Juan Pablo ; Wauben, Marca H. M. ; Witwer, Kenneth W. ; Nolte-’t Hoen, Esther N. M.
The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different in vitro cell systems and in a variety of body fluids. RNA-containing EV are in the limelight for their capacity to communicate genetically encoded messages to other cells, their suitability as candidate biomarkers for diseases, and their use as therapeutic agents. Although EV-RNA has attracted enormous interest from basic researchers, clinicians, and industry, we currently have limited knowledge on which mechanisms drive and regulate RNA incorporation into EV and on how RNA-encoded messages affect signalling processes in EV-targeted cells. Moreover, EV-RNA research faces various technical challenges, such as standardisation of EV isolation methods, optimisation of methodologies to isolate and characterise minute quantities of RNA found in EV, and development of approaches to demonstrate functional transfer of EV-RNA in vivo. These topics were discussed at the 2015 EV-RNA workshop of the International Society for Extracellular Vesicles. This position paper was written by the participants of the workshop not only to give an overview of the current state of knowledge in the field, but also to clarify that our incomplete knowledge – of the nature of EV(-RNA)s and of how to effectively and reliably study them – currently prohibits the implementation of gold standards in EV-RNA research. In addition, this paper creates awareness of possibilities and limitations of currently used strategies to investigate EV-RNA and calls for caution in interpretation of the obtained data.
Théry, Clotilde ; Witwer, Kenneth W. ; Aikawa, Elena ; Alcaraz, Maria Jose ; Anderson, Johnathon D. ; Andriantsitohaina, Ramaroson ; Antoniou, Anna ; Arab, Tanina ; Archer, Fabienne ; Atkin-Smith, Georgia K. ; Ayre, D. Craig ; Bach, Jean-Marie ; Bachurski, Daniel ; Baharvand, Hossein ; Balaj, Leonora ; Baldacchino, Shawn ; Bauer, Natalie N. ; Baxter, Amy A. ; Bebawy, Mary ; Beckham, Carla ; Zavec, Apolonija Bedina ; Benmoussa, Abderrahim ; Berardi, Anna C. ; Bergese, Paolo ; Bielska, Ewa ; Blenkiron, Cherie ; Bobis-Wozowicz, Sylwia ; Boilard, Eric ; Boireau, Wilfrid ; Bongiovanni, Antonella ; Borràs, Francesc E. ; Bosch, Steffi ; Boulanger, Chantal M. ; Breakefield, Xandra ; Breglio, Andrew M. ; Brennan, Meadhbh Aín ; Brigstock, David R. ; Brisson, Alain ; Broekman, Marike L. D. ; Bromberg, Jacqueline F. ; Bryl-Gorecka, Paulina ; Buch, Shilpa ; Buck, Amy H. ; Burger, Dylan ; Busatto, Sara ; Buschmann, Dominik ; Bussolati, Benedetta ; Buzás, Edit I. ; Byrd, James Bryan ; Camussi, Giovanni ; Carter, David R. F. ; Caruso, Sarah ; Chamley, Lawrence W. ; Chang, Yu-Ting ; Chen, Chihchen ; Chen, Shuai ; Cheng, Lesley ; Chin, Andrew R. ; Clayton, Aled ; Clerici, Stefano P. ; Cocks, Alex ; Cocucci, Emanuele ; Coffey, Robert J. ; Cordeiro-da-Silva, Anabela ; Couch, Yvonne ; Coumans, Frank A. W. ; Coyle, Beth ; Crescitelli, Rossella ; Criado, Miria Ferreira ; D’Souza-Schorey, Crislyn ; Das, Saumya ; Chaudhuri, Amrita Datta ; de-Candia, Paola ; De Santana, Eliezer F. ; De Wever, Olivier ; del Portillo, Hernando A. ; Demaret, Tanguy ; Deville, Sarah ; Devitt, Andrew ; Dhondt, Bert ; Di Vizio, Dolores ; Dieterich, Lothar C. ; Dolo, Vincenza ; Dominguez Rubio, Ana Paula ; Dominici, Massimo ; Dourado, Mauricio R. ; Driedonks, Tom A. P. ; Duarte, Filipe V. ; Duncan, Heather M. ; Eichenberger, Ramon Marc ; Ekström, Karin ; El Andaloussi, Samir ; Elie-Caille, Celine ; Erdbrügger, Uta ; Falcón-Pérez, Juan M. ; Fatima, Farah ; Fish, Jason E. ; Flores-Bellver, Miguel ; Försönits, András ; Frelet-Barrand, Annie ; Fricke, Fabia ; Fuhrmann, Gregor ; Gabrielsson, Susanne ; Gámez-Valero, Ana ; Gardiner, Chris ; Gärtner, Kathrin ; Gaudin, Raphael ; Gho, Yong Song ; Giebel, Bernd ; Gilbert, Caroline ; Gimona, Mario ; Giusti, Ilaria ; Goberdhan, Deborah C. I. ; Görgens, André ; Gorski, Sharon M. ; Greening, David W. ; Groß, Julia Christina ; Gualerzi, Alice ; Gupta, Gopal N. ; Gustafson, Dakota ; Handberg, Aase ; Haraszti, Réka Ágnes ; Harrison, Paul ; Hegyesi, Hargita ; Hendrix, An ; Hill, Andrew F. ; Hochberg, Fred H. ; Hoffmann, Karl F. ; Holder, Beth ; Holthofer, Harry ; Hosseinkhani, Baharak ; Hu, Guoku ; Huang, Yiyao ; Huber, Veronica ; Hunt, Stuart ; Ibrahim, Ahmed Gamal-Eldin ; Ikezu, Tsuneya ; Inal, Jameel M. ; Isin, Mustafa ; Ivanova, Alena ; Jackson, Hannah K. ; Jacobsen, Soren ; Jay, Steven M. ; Jayachandran, Muthuvel ; Jenster, Guido ; Jiang, Lanzhou ; Johnson, Suzanne M. ; Jones, Jennifer C. ; Jong, Ambrose ; Jovanovic-Talisman, Tijana ; Jung, Stephanie ; Kalluri, Raghu ; Kano, Shin-ichi ; Kaur, Sukhbir ; Kawamura, Yumi ; Keller, Evan T. ; Khamari, Delaram ; Khomyakova, Elena ; Khvorova, Anastasia ; Kierulf, Peter ; Kim, Kwang Pyo ; Kislinger, Thomas ; Klingeborn, Mikael ; Klinke, David J. ; Kornek, Miroslaw ; Kosanović, Maja M. ; Kovács, Árpád Ferenc ; Krämer-Albers, Eva-Maria ; Krasemann, Susanne ; Krause, Mirja ; Kurochkin, Igor V. ; Kusuma, Gina D. ; Kuypers, Sören ; Laitinen, Saara ; Langevin, Scott M. ; Languino, Lucia R. ; Lannigan, Joanne ; Lässer, Cecilia ; Laurent, Louise C. ; Lavieu, Gregory ; Lázaro-Ibáñez, Elisa ; Le Lay, Soazig ; Lee, Myung-Shin ; Lee, Yi Xin Fiona ; Lemos, Debora S. ; Lenassi, Metka ; Leszczynska, Aleksandra ; Li, Isaac T. S. ; Liao, Ke ; Libregts, Sten F. ; Ligeti, Erzsebet ; Lim, Rebecca ; Lim, Sai Kiang ; Linē, Aija ; Linnemannstöns, Karen ; Llorente, Alicia ; Lombard, Catherine Ann ; Lorenowicz, Magdalena J. ; Lörincz, Ákos M. ; Lötvall, Jan ; Lovett, Jason ; Lowry, Michelle C. ; Loyer, Xavier ; Lu, Quan ; Lukomska, Barbara ; Lunavat, Taral Rameshchand ; Maas, Sybren L. N. ; Malhi, Harmeet ; Marcilla, Antonio ; Mariani, Jacopo ; Mariscal, Javier ; Martens-Uzunova, Elena S. ; Martin-Jaular, Lorena ; Martinez, M. Carmen ; Martins, Vilma Regina ; Mathieu, Mathilde ; Mathivanan, Suresh ; Maugeri, Marco ; McGinnis, Lynda K. ; McVey, Mark J. ; Meckes, David G. ; Meehan, Katie ; Mertens, Inge ; Minciacchi, Valentina R. ; Möller, Andreas ; Møller Jørgensen, Malene ; Morales-Kastresana, Aizea ; Morhayim, Jess ; Mullier, François ; Muraca, Maurizio ; Musante, Luca ; Mussack, Veronika ; Muth, Dillon C. ; Myburgh, Kathryn H. ; Najrana, Tanbir ; Nawaz, Muhammad ; Nazarenko, Irina ; Nejsum, Peter ; Neri, Christian ; Neri, Tommaso ; Nieuwland, Rienk ; Nimrichter, Leonardo ; Nolan, John P. ; Nolte-’t Hoen, Esther N. M. ; Hooten, Nicole Noren ; O’Driscoll, Lorraine ; O’Grady, Tina ; O’Loghlen, Ana ; Ochiya, Takahiro ; Olivier, Martin ; Ortiz, Alberto ; Ortiz, Luis A. ; Osteikoetxea, Xabier ; Østergaard, Ole ; Ostrowski, Matias ; Park, Jaesung ; Pegtel, Dirk Michiel ; Peinado, Hector ; Perut, Francesca ; Pfaffl, Michael ; Phinney, Donald G. ; Pieters, Bartijn C. H. ; Pink, Ryan Charles ; Pisetsky, David S. ; Pogge von Strandmann, Elke ; Polakovicova, Iva ; Poon, Ivan K. H. ; Powell, Bonita H. ; Prada, Ilaria ; Pulliam, Lynn ; Quesenberry, Peter ; Radeghieri, Annalisa ; Raffai, Robert L. ; Raimondo, Stefania ; Rak, Janusz ; Ramirez, Marcel I. ; Raposo, Graça ; Rayyan, Morsi S. ; Regev-Rudzki, Neta ; Ricklefs, Franz Lennard ; Robbins, Paul D. ; Roberts, David D. ; Rodrigues, Silvia C. ; Rohde, Eva ; Rome, Sophie ; Rouschop, Kasper M. ; Rughetti, Aurelia ; Russell, Ashley E. ; Saá, Paula ; Sahoo, Susmita ; Salas-Huenuleo, Edison ; Sánchez, Catherine ; Saugstad, Julie A. ; Saul, Meike J. ; Schiffelers, Raymond M. ; Schneider, Raphael ; Schøyen, Tine Hiorth ; Scott, Aaron ; Shahaj, Eriomina ; Sharma, Shivani ; Shatnyeva, Olga ; Shekari, Faezeh ; Shelke, Ganesh Vilas ; Shetty, Ashok K. ; Shiba, Kiyotaka ; Siljander, Pia R.-M. ; Silva, Andreia M. ; Skowronek, Agata ; Snyder, Orman L. ; Soares, Rodrigo Pedro ; Sódar, Barbara W. ; Soekmadji, Carolina ; Sotillo, Javier ; Stahl, Philip D. ; Stoorvogel, Willem ; Stott, Shannon L. ; Strasser, Erwin F. ; Swift, Simon ; Tahara, Hidetoshi ; Tewari, Muneesh ; Timms, Kate ; Tiwari, Swasti ; Tixeira, Rochelle ; Tkach, Mercedes ; Toh, Wei Seong ; Tomasini, Richard ; Torrecilhas, Ana Claudia ; Tosar, Juan Pablo ; Toxavidis, Vasilis ; Urbanelli, Lorena ; Vader, Pieter ; Balkom, Bastiaan Wilhelmus Maria van ; Grein, Susanne G. van der ; Van Deun, Jan ; Herwijnen, Martijn J. C. van ; Van Keuren-Jensen, Kendall R. ; Niel, Guillaume van ; Royen, Martin E. van ; Wijnen, Andre J. van ; Vasconcelos, M. Helena ; Vechetti, Ivan J. ; Veit, Tiago D. ; Vella, Laura J. ; Velot, Émilie ; Verweij, Frederik ; Vestad, Beate ; Viñas, Jose Luis ; Visnovitz, Tamás ; Vukman, Krisztina V. ; Wahlgren, Jessica ; Watson, Dionysios C. ; Wauben, Marca H. M. ; Weaver, Alissa ; Webber, Jason P. ; Weber, Viktoria ; Wehman, Ann M. ; Weiss, Daniel J. ; Welsh, Joshua A. ; Wendt, Sebastian ; Wheelock, Åsa M. ; Wiener, Zoltán ; Witte, Leonie ; Wolfram, Joy ; Xagorari, Angeliki ; Xander, Patricia ; Xu, Jing ; Yan, Xiaomei ; Yáñez-Mó, María ; Yin, Hang ; Yuana, Yuana ; Zappulli, Valentina ; Zarubova, Jana ; Žėkas, Vytautas ; Zhang, Jian-ye ; Zhao, Zezhou ; Zheng, Lei ; Zheutlin, Alexander R. ; Zickler, Antje Maria ; Zimmermann, Pascale ; Zivkovic, Angela M. ; Zocco, Davide ; Zuba-Surma, Ewa K.
The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
- 1 to 3