Refine
Year of publication
Document Type
- Article (104)
- Preprint (9)
- Conference Proceeding (4)
- Contribution to a Periodical (3)
- Book (1)
- Working Paper (1)
Has Fulltext
- yes (122)
Is part of the Bibliography
- no (122)
Keywords
- COVID-19 (5)
- SARS-CoV-2 (4)
- Biomarkers (3)
- ACLF (2)
- Cirrhosis (2)
- Genetics (2)
- HCC (2)
- Immunology (2)
- Inflammation (2)
- NASH (2)
- risk factors (2)
- 2‑methoxyestradiol (1)
- ALK-rearranged NSCLC (1)
- AML (1)
- Acute inflammation (1)
- Acute myeloid leukemia (1)
- Advanced stage (1)
- Alzheimer’s dementia (1)
- Amyloid-beta 42 (1)
- Anal cancer (1)
- Antibiotic therapy (1)
- Artificial Intelligence (1)
- Assay variation (1)
- Aurora kinase (1)
- BCOR (1)
- BCORL1 (1)
- Biodiversity Data (1)
- Biomonitoring (1)
- Botanical Collections (1)
- Brain tumor (1)
- Burden of disease (1)
- CEP68 (1)
- CGD (1)
- CLIF-C ACLF score (1)
- CLIF-C ACLF-R score (1)
- COBAS Taqman (1)
- COMP (1)
- COVID 19 (1)
- CRISPR/Cas9 (1)
- CVD biomarker (1)
- Cancer (1)
- Cardiovascular disease (1)
- Cardiovascular disease risk (1)
- Cardiovascular diseases (1)
- Cell biology (1)
- Clinical frailty scale (1)
- Cofactors (biochemistry) (1)
- Colorectal cancer (1)
- Complicated stage (1)
- Computerlinguistik (1)
- Concurrent chemoradiotherapy (1)
- Conservation (1)
- Data sharing (1)
- Detergents (1)
- Devic disease (1)
- Devic syndrome (1)
- Devisenmarkt (1)
- Diabetes mellitus (1)
- Diagnostic markers (1)
- Digestive system (1)
- Digitization (1)
- EGFL7 (1)
- EWSR1 (1)
- Eignungsprüfung (1)
- Electronic properties and materials (1)
- Endoscopy (1)
- Experimental models of disease (1)
- Fluid overload (1)
- Frailty (1)
- Freundschaft, Begriff (1)
- Frühgeborenes (1)
- G-protein (1)
- GARCH-Prozess (1)
- Gait analysis (1)
- Gastfreundschaft (1)
- Gastfreundschaft <Motiv> (1)
- Gastfreundschaft, Begriff (1)
- Gene fusion (1)
- Generic NLP Architecture (1)
- Gomphus flavipes (1)
- HCV (1)
- HPSG Parsing (1)
- Health care resource utilization (1)
- Heme (1)
- Herbaria (1)
- Hippocampal volume (1)
- Hypertension (1)
- IE (1)
- Immunogenetics (1)
- Induction chemotherapy (1)
- Intensive care outcome (1)
- Intensive care treatment (1)
- Intestinal neoplasms (1)
- Joint loading (1)
- Kaukasische Völker (1)
- Kindheit (1)
- Kulturbeziehungen (1)
- Kunst (1)
- LEOSS (1)
- Libellen (1)
- Literatur (1)
- Liver diseases (1)
- Low birth weight infant (1)
- MN1 (1)
- MR-proADM (1)
- Machine learning (1)
- Magnetic properties and materials (1)
- Mechanisms of disease (1)
- Medizinstudium (1)
- Membrane proteins (1)
- Metabolic syndrome (1)
- Mild cognitive impairment (1)
- Molecular biology (1)
- Molecular marker (1)
- Monitoring (1)
- Mortality (1)
- Multiple choice Fragen (1)
- Multivariate analysis (1)
- NAFLD (1)
- NHEJ (1)
- NMO-IgG (1)
- NMR spectroscopy (1)
- Nephrons (1)
- Neugeborenes (1)
- Neuroepithelial (1)
- Neuromyelitis optica (1)
- Neurooncology (1)
- Obesity (1)
- Oncogenes (1)
- Oncology (1)
- Ophiogomphus cecilia (1)
- Orphan disease (1)
- Osteoarthritis (1)
- PATZ1 (1)
- Pediatric (1)
- Periodontal disease (1)
- Periodontal therapy (1)
- Phase transitions and critical phenomena (1)
- Phospho-tau (1)
- Plasminogen (1)
- Platelets (1)
- Portal veins (1)
- Prediction (1)
- Predictive model (1)
- Prostaglandin (1)
- Proteases (1)
- Protein structure (1)
- Proteolysis (1)
- Prüfungserfolg (1)
- Psychiatric disorders (1)
- RNA (1)
- RNA stem-loop structure (1)
- RNA-binding proteins (1)
- Radical nephrectomy (1)
- Ratewahrscheinlichkeit (1)
- RealTime (1)
- Renal cancer (1)
- Renal system (1)
- Research Infrastructure (1)
- Research article (1)
- Respimat® Soft Mist™ Inhaler (1)
- Respiratory signs and symptoms (1)
- Rho/Rho-kinase (1)
- S100 calcium‑binding protein B (1)
- SARS CoV 2 (1)
- SOFA (1)
- STAR GARCH (1)
- STED superresolution (1)
- STK3 (1)
- Self-expandable metal stents (1)
- Self-expandable metal stents complications (1)
- Semantics (1)
- Sepsis (1)
- Septic shock (1)
- Serum biomarker (1)
- Shallow NLP (1)
- Soft skills (1)
- Sowjetunion (1)
- Surgical and invasive medical procedures (1)
- Surgical oncology (1)
- Survival (1)
- Survival analysis (1)
- Survivin (1)
- TACE (1)
- TP53 mutation status (1)
- Tau (1)
- Taxonomy (1)
- Thrombosis (1)
- Thromboxane (1)
- Total hip arthroplasty (1)
- Translational research (1)
- Trauma (1)
- Tumor obstruction (1)
- UCN-01 (1)
- VIP1 (1)
- Validität (1)
- Veins (1)
- Viral load (1)
- Völkerverständigung (1)
- Wechselkurs (1)
- X-ray crystallography (1)
- X-ray structure analysis (1)
- XML (1)
- Zulassungskriterien (1)
- accessory proteins (1)
- acute myeloid leukemia (1)
- acute-on-chronic liver failure (1)
- admission criteria (1)
- admission test (1)
- advanced care planning (1)
- angiogenesis (1)
- anilinopyrimidine (1)
- aquaporin-4 (AQP4) antibody (1)
- bacterial translocation (1)
- benzazepinone (1)
- cell-free protein synthesis (1)
- centrosome (1)
- centrosome linker (1)
- cerebrospinal fluid (1)
- childhood (1)
- chronic granulomatous disease (1)
- cirrhosis (1)
- clinical features (1)
- comorbidities (1)
- critically ill patients (1)
- day clinic (1)
- device handling (1)
- edentulous (1)
- endothelial cell (1)
- epidemiology (1)
- examination success (1)
- fibrosis (1)
- full-arch restoration (1)
- glioblastoma (1)
- global change (1)
- guessing likelihood (1)
- habitat destruction (1)
- hematopoietic cells (1)
- hospital admission (1)
- human biology and medicine (1)
- in situ gene correction (1)
- inflammation (1)
- inhalation flow profiles (1)
- integrin (1)
- intrinsically disordered region (1)
- lactate dehydrogenase (1)
- land use (1)
- lasso regression (1)
- learning strategies (1)
- longitudinally extensive transverse myelitis (1)
- loss-of-function (1)
- low birth weight infant (1)
- magnetic resonance imaging (1)
- male (1)
- mechanical ventilation (1)
- medical studies (1)
- melanoma (1)
- mitosis (1)
- molecular docking (1)
- monitoring (1)
- mortality (1)
- mouse (1)
- multimodal complex treatment (1)
- multiple choice question (1)
- myogenic tone (1)
- myogenic vasoconstriction (1)
- networks (1)
- neurological manifestations (1)
- neutralizing antibodies (1)
- newborn infant (1)
- nomogram (1)
- non-homologous end joining (1)
- nonlinearities (1)
- nonstructural proteins (1)
- offene Fragen (1)
- peri-implant disease (1)
- personalized medicine (1)
- premature infant (1)
- preschool children (1)
- prevalence (1)
- prognostic factor (1)
- protein kinase inhibitor (1)
- pulmonary failure (1)
- recurrent optic neuritis (1)
- respiratory disease (1)
- respiratory failure (1)
- risk factor progression (1)
- risk stratification (1)
- rootletin (1)
- sequential ALK-inhibitor therapy (1)
- short essay questions (1)
- signal transduction (1)
- single best choice question (1)
- soft skills (1)
- sorafenib (1)
- spike protein (1)
- stage (1)
- structural proteins (1)
- studentisches Lernverhalten (1)
- sulfamide (1)
- survival (1)
- systematic review (1)
- technical and fundamental trading (1)
- treatment (1)
- validity (1)
- variants of concern (1)
- vascular smooth muscle (1)
Institute
- Medizin (64)
- Physik (34)
- Biochemie, Chemie und Pharmazie (6)
- Biowissenschaften (5)
- Biochemie und Chemie (3)
- Pharmazie (3)
- Präsidium (3)
- Senckenbergische Naturforschende Gesellschaft (3)
- Biodiversität und Klima Forschungszentrum (BiK-F) (2)
- Zentrum für Arzneimittelforschung, Entwicklung und Sicherheit (ZAFES) (2)
Combined diabetes-obesity syndromes severely impair regeneration of acute skin wounds in mouse models. This study assessed the contribution of subcutaneous adipose tissue to exacerbated wound inflammatory conditions. Genetically obese (ob/ob) mice showed an increased expression of positive transcriptional effectors of adipocyte differentiation such as Krüppel-like factor (KLF)-5 and peroxisome proliferator-activated receptor (PPAR)-γ and an associated expression of leptin and fatty acid-binding protein (FABP)-4, but also CXCL2 in isolated subcutaneous fat. This observation in obese mice is in keeping with differentially elevated levels of KLF-5, PPAR-γ, leptin, FABP-4 and CXCL2 in in vitro-differentiated 3T3-L1 adipocytes. Notably, CXCL2 expression restrictively appeared upon cytokine (IL-1β/TNF-α) stimulation only in mature, but not immature 3T3-L1 adipocytes. Of importance, the critical regulator of adipocyte maturation, PPAR-γ, was merely expressed in the final phase of in-vitro induced adipocyte differentiation from 3T3-L1 pre-adipocytes. Consistently, the PPAR-γ agonist rosiglitazone suppressed cytokine-induced CXCL2 release from mature adipocytes, but not from early 3T3-L1 adipocyte stages. The inhibitory effect of PPAR-γ activation on CXCL2 release appeared to be a general anti-inflammatory effect in mature adipocytes, as cytokine-induced cyclooxygenase (Cox)-2 was simultaneously repressed by rosiglitazone. In accordance with these findings, oral administration of rosiglitazone to wounded obese mice significantly changed subcutaneous adipocyte morphology, reduced wound CXCL2 and Cox-2 expression and improved tissue regeneration. Thus, our data suggest that PPAR-γ might provide a target to suppress inflammatory signals from mature adipocytes, which add to the prolonged wound inflammation observed in diabetes-obesity conditions.
Within the SEASTAR project at RIKEN-RIBF, 66Cr and 70,72Fe have been produced via protonknockout reactions, and their first excited 2+ and 4+ states have been discovered. The combination of the liquid-hydrogen target and TPC system MINOS has been used in combination with the DALI2 detector array for the first time. A 345 MeV/u 238U beam with a mean intensity of about 12 pnA impinged on a Be target. Fission fragments were separated and identified using the BigRIPS spectrograph, and reaction products were analyzed using the ZeroDegree spectrograph. A plateau of excitation energies, with a small change in the systematic trends past N = 44, reveals an extension of the N = 40 region of collectivity toward N = 50. Hence, the isotopes of interest are located within the N = 40 island of inversion. An interpretation of the observed trends is offered through large scale shell model calculations.
Purpose: While more advanced COVID-19 necessitates medical interventions and hospitalization, patients with mild COVID-19 do not require this. Identifying patients at risk of progressing to advanced COVID-19 might guide treatment decisions, particularly for better prioritizing patients in need for hospitalization.
Methods: We developed a machine learning-based predictor for deriving a clinical score identifying patients with asymptomatic/mild COVID-19 at risk of progressing to advanced COVID-19. Clinical data from SARS-CoV-2 positive patients from the multicenter Lean European Open Survey on SARS-CoV-2 Infected Patients (LEOSS) were used for discovery (2020-03-16 to 2020-07-14) and validation (data from 2020-07-15 to 2021-02-16).
Results: The LEOSS dataset contains 473 baseline patient parameters measured at the first patient contact. After training the predictor model on a training dataset comprising 1233 patients, 20 of the 473 parameters were selected for the predictor model. From the predictor model, we delineated a composite predictive score (SACOV-19, Score for the prediction of an Advanced stage of COVID-19) with eleven variables. In the validation cohort (n = 2264 patients), we observed good prediction performance with an area under the curve (AUC) of 0.73 ± 0.01. Besides temperature, age, body mass index and smoking habit, variables indicating pulmonary involvement (respiration rate, oxygen saturation, dyspnea), inflammation (CRP, LDH, lymphocyte counts), and acute kidney injury at diagnosis were identified. For better interpretability, the predictor was translated into a web interface.
Conclusion: We present a machine learning-based predictor model and a clinical score for identifying patients at risk of developing advanced COVID-19.
Systemic inflammation is associated with alterations in complex brain functions such as learning and memory. However, diagnostic approaches to functionally assess and quantify inflammation-associated alterations in synaptic plasticity are not well-established. In previous work, we demonstrated that bacterial lipopolysaccharide (LPS)-induced systemic inflammation alters the ability of hippocampal neurons to express synaptic plasticity, i.e., the long-term potentiation (LTP) of excitatory neurotransmission. Here, we tested whether synaptic plasticity induced by repetitive magnetic stimulation (rMS), a non-invasive brain stimulation technique used in clinical practice, is affected by LPS-induced inflammation. Specifically, we explored brain tissue cultures to learn more about the direct effects of LPS on neural tissue, and we tested for the plasticity-restoring effects of the anti-inflammatory cytokine interleukin 10 (IL10). As shown previously, 10 Hz repetitive magnetic stimulation (rMS) of organotypic entorhino-hippocampal tissue cultures induced a robust increase in excitatory neurotransmission onto CA1 pyramidal neurons. Furthermore, LPS-treated tissue cultures did not express rMS-induced synaptic plasticity. Live-cell microscopy in tissue cultures prepared from a novel transgenic reporter mouse line [C57BL/6-Tg(TNFa-eGFP)] confirms that ex vivo LPS administration triggers microglial tumor necrosis factor alpha (TNFα) expression, which is ameliorated in the presence of IL10. Consistent with this observation, IL10 hampers the LPS-induced increase in TNFα, IL6, IL1β, and IFNγ and restores the ability of neurons to express rMS-induced synaptic plasticity in the presence of LPS. These findings establish organotypic tissue cultures as a suitable model for studying inflammation-induced alterations in synaptic plasticity, thus providing a biological basis for the diagnostic use of transcranial magnetic stimulation in the context of brain inflammation.
Nuclear factor of activated T-cells (NFAT) and NF-kB pathway associated processes are involved in the pathogenesis of various inflammatory disorders, for example, periodontal disease. The activation of these pathways is controlled by the regulator of calcineurin 1 (RCAN1). The aim of this study was to elucidate the role of RCAN1 in periodontal disease. Healthy and inflamed periodontal tissues were analyzed by immunohistochemistry and immunofluorescence using specific rabbit polyclonal anti-RCAN1 antibodies. For expression analysis human umbilical vein endothelial cells (HUVEC) were used. HUVEC were incubated for 2 h with Vascular Endothelial Growth Factor (VEGF) or with wild type and laboratory strains of Porphyromonas gingivalis (P. gingivalis). Expression analysis of rcan1 and cox2 was done by real time PCR using specific primers for rcan1.4 and cox2. The expression of rcan1 was found to be significantly suppressed in endothelial cells of chronically inflamed periodontal tissues compared to healthy controls. Rcan1 and cox2 were significantly induced by VEGF and wild type and laboratory P. gingivalis strains. Interestingly, the magnitude of the rcan1 and cox2 induction was strain dependent. The results of this study indicate that RCAN1 is suppressed in endothelial cells of chronically inflamed periodontal tissues. During an acute infection, however, rcan1 seems to be upregulated in endothelial cells, indicating a modulating role in immune homeostasis of periodontal tissues.
Large-scale molecular profiling studies in recent years have shown that central nervous system (CNS) tumors display a much greater heterogeneity in terms of molecularly distinct entities, cellular origins and genetic drivers than anticipated from histological assessment. DNA methylation profiling has emerged as a useful tool for robust tumor classification, providing new insights into these heterogeneous molecular classes. This is particularly true for rare CNS tumors with a broad morphological spectrum, which are not possible to assign as separate entities based on histological similarity alone. Here, we describe a molecularly distinct subset of predominantly pediatric CNS neoplasms (n = 60) that harbor PATZ1 fusions. The original histological diagnoses of these tumors covered a wide spectrum of tumor types and malignancy grades. While the single most common diagnosis was glioblastoma (GBM), clinical data of the PATZ1-fused tumors showed a better prognosis than typical GBM, despite frequent relapses. RNA sequencing revealed recurrent MN1:PATZ1 or EWSR1:PATZ1 fusions related to (often extensive) copy number variations on chromosome 22, where PATZ1 and the two fusion partners are located. These fusions have individually been reported in a number of glial/glioneuronal tumors, as well as extracranial sarcomas. We show here that they are more common than previously acknowledged, and together define a biologically distinct CNS tumor type with high expression of neural development markers such as PAX2, GATA2 and IGF2. Drug screening performed on the MN1:PATZ1 fusion-bearing KS-1 brain tumor cell line revealed preliminary candidates for further study. In summary, PATZ1 fusions define a molecular class of histologically polyphenotypic neuroepithelial tumors, which show an intermediate prognosis under current treatment regimens.
Purpose: To investigate the prognostic value of survivin expression in pretreatment specimens from patients with anal cancer treated with concurrent 5-FU and mitomycin C-based chemoradiation (CRT).
Material and methods: Immunohistochemical staining for survivin was performed in pretreatment biopsies of 62 patients with anal carcinoma. Survivin expression was correlated with clinical and histopathological characteristics as well as local failure free- (LFFS), distant metastases free- (DMFS), cancer specific- (CSS), and overall survival (OS).
Results: Survivin staining intensity was weak in 10%, intermediate in 48% and intense in 42% of the patients. No association between survivin expression and clinicopathologic factors (tumor stage, age and HIV status) could be shown. In univariate analysis, the level of survivin staining was significantly correlated with DMFS (low survivin vs. high survivin: 94% vs. 74%, p=0.04). T-stage, N-stage and the tumor grading were significantly associated with OS and CSS and with DMFS and LFFS, respectively. In multivariate analysis, survivin was confirmed as independent prognostic parameter for DMFS (RR, 0.04; p=0.02) and for OS (RR, 0.27; p=0.04).
Conclusion: Our results demonstrated that the level of pretreatment survivin is correlated with the clinical outcome in patients with anal carcinoma treated with concurrent CRT. Further studies are warranted to elucidate the complex role of survivin for the oncologic treatment and to exploit the protein as a therapeutic target in combined modality treatment of anal cancer.
Introns of human transfer RNA precursors (pre-tRNAs) are excised by the tRNA splicing endonuclease TSEN in complex with the RNA kinase CLP1. Mutations in TSEN/CLP1 occur in patients with pontocerebellar hypoplasia (PCH), however, their role in the disease is unclear. Here, we show that intron excision is catalyzed by tetrameric TSEN assembled from inactive heterodimers independently of CLP1. Splice site recognition involves the mature domain and the anticodon-intron base pair of pre-tRNAs. The 2.1-Å resolution X-ray crystal structure of a TSEN15–34 heterodimer and differential scanning fluorimetry analyses show that PCH mutations cause thermal destabilization. While endonuclease activity in recombinant mutant TSEN is unaltered, we observe assembly defects and reduced pre-tRNA cleavage activity resulting in an imbalanced pre-tRNA pool in PCH patient-derived fibroblasts. Our work defines the molecular principles of intron excision in humans and provides evidence that modulation of TSEN stability may contribute to PCH phenotypes.