540 Chemie und zugeordnete Wissenschaften
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Chromosomale Strukturen von Pseudomonas testosteroni. II. Aktivität der endogenen RNA-Polymerase
(1976)
After careful lysis the nucleoid of Pseudomonas testosteroni can be isolated in three different forms with compact and unfolded DNA structures 1. The released nucleoids contain endogenous DNA-dependent RNA-polymerase activity using the chromosomal DNA as a template. RNA syn thesis is proportional to duration of RNA-polymerase reaction and amount of DNA-protein-complexes. The sensitivity towards ionic strength and rifampicin indicates that a part of RNA-polymerase activity is tightly bound to the chromosomal DNA.