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- Androst-4-en-3,17-dione (1)
- DNA-Specific Labelling (1)
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Die Bedeutung des Films als Meßmethode entspricht der Relevanz von Bewegungsvorgängen im Rahmen der wissenschaftlichen Problemstellungen, Die bisherigen Auswertverfahren lassen einen großen Teil der im Film gespeicherten Information unausgenutzt und sind zudem sehr zeitaufwendig, weshalb die Analyse moist unterbleibt. Eino Automatisierung des Auswertvorganges setzt, eine Anpassung von Aufnahmebedingungen und Problemstellung an die spezifischen Eigenschaften des Analyseverfahrens voraus. Verschiedene Stufen der Komplexität von Bewegungsphänomenen werden erläutert im Hinblick auf eine veränderte Betrachtungsweise, wie sie für die Aufbereitung von Problemen zur Bearbeitung durch Bildanalysegeräte erfolgen muß.
In haploid and diploid S. cerevisiae the dimer yield ratio TT̂/CT̂ is found to be 1.2/1 and 1.3/1, resp., at the UV (254 nm) unit dose 1 erg/mm2, the share of TT̂ and CT̂ in a UV (254 nm) lethal hit being 0.7 TT̂ and 0.6 CT̂. A general formulation of the UV lethal hit is given and discussed. The TT̂ + CT̂ yields obtained for S. cerevisiae are compared to those reported for other organisms. It is found that there obviously exists a directly proportional linear correlation between genome size and TT̂ + CT̂ yield for the UV dose range well below the stationary levels of the TT̂ and CT̂ formation kinetics.
Testosterone, Androst-4-en-3,17-dione, Enzyme Induction, S trep to m yces hydrogenans After cultivation of S trep to m yces hydrogenan s in the presence of 3H-labelled testosterone, radio active steroids were extracted separately from the cytosolic, ribosomal and cell wall-membrane fraction of the cells and from the culture medium, respectively.. The separation of the steroids was performed by one-and two-dimensional thin layer chromatography (TLC). The identification of the main metabolites was achieved by crystallization to constant specific radioactivity, specific staining procedures and acetylation. The oxidation of testosterone to androst-4-en-3,17-dione is by far the predominating reaction, which is almost finished after 3 h cultivation. Androst-4-en-3,17-dione is mainly transferred into the culture medium and partly accumulated within the cell wall-membrane fraction. High polar steroid metabolites and androstane derivatives are present in very small amounts only.