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Steroid initiated enzyme induction (Δ5-Ketosteroid-Isomerase, 3α-Hydroxysteroid-Dehydrogenase, and 3β.17β-Hydroxysteroid-Dehydrogenase) in Pseudomonas testosteroni was investigated with respect to the kinetics of induction, operon control of the induced enzymes, and the relative strengths of various inducers. The induction process was followed indirectly by selective inhibition of different stages in the protein synthetic pathway. Comparisons between bacterial and mammalian steroid induction are discussed.
The non-specific inhibition of the poly U directed polymerisation of phenylalanine through polyanions was studied. This inhibition was found to be in order as follows: dextransulfate, polyethylensulfate, heparine, ribosomal RNA and alginate. It was found that poly A, poly AP and poly AG cause a specific inhibition of the poly U directed synthesis of polyphenylalanine. Poly AG and poly AP, but not poly A were found to inhibit the poly C directed polymerisation of proline as well. The mechanism of these two types of inhibition caused by polyanions has been discussed.
A study on the effect of UV-irradiated polyuridylic acid on the incorporation of phenylalanine into the polypeptide precipitable through trichloroacetic acid, in a cell-free system from E. coli was made. Attempts were made to reactivate the UV-inactivated polyuridylic acid through hydrogen peroxide, uranyl acetate and visible light. We could show that polyuridylic acid irradiated at a dose of 1.2 ×105 ergs/mm2 could be completely reactivated, while the one irradiated at a higher dose of 2.4 ×105 ergs/mm2 could not be completely reactivated under the conditions of our experiment. We have studied the effects of hydrogen peroxide and uranyl acetate on UV-irradiated polyuridylic acid chemically as well. Our results altogether show that the photoreactivating effect of uranyl acetate and hydrogen peroxide is due to their ability to split the uracil dimers formed during UV-irradiation.
Es wurden mehrere unkonjugierte 2.4-Diaminopteridine erstmals synthetisiert. Die Wachstumshemmung verschiedener Mikroorganismen durch 2.4-Diamino-6-[1.2-dihydroxypropyl-(ʟ-erythro)] pteridin (Aminobiopterin) und anderer unkonjugierter 2.4-Diamino-pteridine läßt sich nur mit Folsäure oder Thymin, nicht dagegen mit Biopterin aufheben.