Refine
Year of publication
Document Type
- Article (31477) (remove)
Language
- English (16082)
- German (13387)
- Portuguese (696)
- French (387)
- Croatian (251)
- Spanish (250)
- Italian (134)
- Turkish (113)
- Multiple languages (36)
- Latin (35)
Has Fulltext
- yes (31477)
Keywords
- Deutsch (503)
- taxonomy (451)
- Literatur (299)
- new species (194)
- Hofmannsthal, Hugo von (185)
- Rezeption (178)
- Übersetzung (163)
- Filmmusik (155)
- Johann Wolfgang von Goethe (131)
- Vormärz (117)
Institute
- Medizin (5409)
- Physik (1959)
- Biowissenschaften (1152)
- Biochemie und Chemie (1113)
- Extern (1108)
- Gesellschaftswissenschaften (803)
- Frankfurt Institute for Advanced Studies (FIAS) (753)
- Geowissenschaften (593)
- Präsidium (453)
- Philosophie (448)
The aim of our project B6 “Towards a genesis of the ethnolinguistic situation at the southern and western fringes of lake Chad basin” within SFB 268 “West African Savannah” is to analyse the emergence and development of the complex presentday ethnolinguistic patterns in a region which may be historically labelled as southern and western periphery of the Borno empire. For the first time, a model of migratory routes was put forward based on combined research efforts of the disciplines involved in our project. Below we shall attempt to summarise the main points and reflections of our findings. Our specific approach as a whole is based on applying the respective research methods of the individual disciplines represented in our project, namely anthropology, ethnomusicology, history and linguistics and eventually on integrating the results into a systemically coherent picture
La présente esquisse vise à montrer l'importance des frontières et des conflits chez les Dagara et leurs voisins du Burkina Faso précolonial. En partant des écrits disponibles et de nos propres enquêtes dans le sud-ouest burkinabè, elle en est arrivée à dégager deux périodes historiques bien distinctes. La première, allant de la fin du XVIIIème siècle à la seconde moitié du XIXè siècle en gros, fut dominée par le dynamisme du front pionnier. L'appropriation de l'espace y engendrait quelques heurts interethniques, voire intra-ethniques, fussentils latents ou ouverts. La deuxième période identifiée, elle, s'étend de la seconde moitié du siècle dernier à la conquête française du sud-ouest burkinabè en 1897-1898. Les Dagara, à l'instar des autres ethnies du sud-ouest sont directement ou indirectement confrontés à des conquérants islamisés parmi lesquels les Zaberma, les troupes samoriennes, et surtout Moctar Karantao de Wahabou. Ce nouveau contexte géopolitique différemment accueilli par les ethnies en place aurait été à l'origine d'une guerre dite de sept ans durant laquelle on note des alliances et pactes interethniques. L'analyse des tactiques et stratégies de ces regroupements ponctuels en temps de crises permet de mieux cerner le comportement des Dagara et des ethnies circonvoisines face à la conquête coloniale.
The paper presents a short introduction to the environmental factors, e.g. climate, geology, relief forms and soils of the study area in the southern parts of the Gongola Basin. The study area covers the high mountain range of the Tangale-Waja Uplands and the adjacent pediplain, following in the north. It is asked if the natural factors enforced former inhabitants of the area to develop special land use techniques like field terracing to ensure the essential crop production under insufficient geoecological conditions.
Die Ergebnisse der Feldforschungen, die hier vorgestellt werden, beruhen auf gemeinsamen Feldaufenthalten der Autoren in dem Untersuchungsgebiet in den Jahren 1990 und 1994. Die ersten Geländebegehungen in diesem Gebiet fanden zusammen mit Günter NAGEL im Februar 1990 statt. Einige seiner Anregungen für interdisziplinäre Arbeiten von Geisteswissenschaftlern und Physischen Geographen zur Landschaftsgenese dieses Raumes, seines Natur- und Nutzungspotentials und der realen aktuellen Inwertsetzung durch die ansässigen Waja wird er, so hoffen die Autoren, in den folgenden Ausführungen wiedererkennen. In dieser Untersuchung wurde vor allem der Frage nachgegangen, welche Beziehungen im südlichen Gongola-Becken zwischen der jüngeren Land-schaftsgenese und dem Natur- und Nutzungspotential bestehen, und wie letzteres von der Bevölkerung in Wert gesetzt wird. Von Interesse sind auch die Auswirkungen der jüngeren Nutzung auf den Naturraum und ihrer Wahrnehmung durch die Bevölkerung.
The Yobe valley is one of the many refugia that dotted the Chad basin after the commencement of the desiccation of the Sahara. It hypothetically must have been attractive to the population that had to move away from the aridized zone in search of favourable ecotones. As the Mega Chad receded from its Bama ridge shores, new lands were progressively made available for human occupation along the valley. It is one of the principal goals of the Yobe Valley Archaeological project to investigate how and when this new valley was occupied. This paper has been divided into three principal sections. The first section deals with the search for the earliest settlements of the Yobe valley. The excavations conducted at Garingada and Damakarwa were aimed at tackling this problem. The second section deals with the development of complexity. The excavation at Gambaru was directed towards this problem. The third section seeks to discuss on the bases of the excavations at the three sites, manenvironment relationship. The concluding part of the paper focuses attention on the problems and prospects of the Yobe Valley Archaeological Project.
Le Burkina Faso est situé dans la zone soudano-sahélienne où les cultures sont quelquefois aléatoires; dans ces conditions les plantes ligneuses occupent une place importante dans la vie socio-économique du pays. Pourtant, une sécheresses climatique persistante de ces dernières décennies a entraîné une mortalité massive de ces ligneux surtout dans la zone sahélienne. Cette forte dégradation des ressources naturelles est variable d’une zone géo-graphique à une autre pour des raisons diverses. Si les phénomènes généraux de cette dégradation de la végétation et leurs causes sont souvent décrits par de nombreux auteurs, il existe par contre très peu de connaissances détaillées et spécifiques en fonction des zones géographiques sur la menace qui pèse sur les différentes espèces. Dans l’absence de bases scientifiques fiables sur la végétation du passé pour chacune des régions qui permettrait ainsi d’établir avec précision la liste des espèces disparues, il est donc nécessaire de se baser sur la perception de la population rurale qui se trouve bien placée pour déceler tout changement dans la flore. Dans ce contexte, cette étude menée auprès des gourmantchés, vise surtout à identifier les espèces disparues ou celles en voie de disparition dans les différentes zones écologiques à l’est du Burkina Faso; en outre, elle aborde les raisons évoquées par ces populations pour expliquer cette menace qui pèse sur chacune des espèces. L’enquête a enfin contribué à connaître les solutions envisagées par ces dernières pour anéantir ou réduire la menace.
Interdépendance du potentiel d'exploitation et la structure d'habitat dans la région de l'Atakora
(2000)
Le département de l’Atakora est une région très contrastée et jusqu’à présent très peu explorée. Son élément le plus saillant est la Chaîne de l’Atakora, une des rares élévations montagneuses de cette partie de l’Afrique occidentale. Le haut-plateau de l’Atakora est caractérisé par une densité de la population relativement basse, comparée au reste de la région, et une végétation variée, qui est jusqu’à présent extrêmement mal connu. Il contraste fortement avec son piémont à l’ouest de la chaîne qui est très densément peuplé et exploité de manière intensive. Notre recherche a été consacrée à la question de savoir si ce sont des facteurs dépendant du géopotentiel ou plutôt des données socio-économiques qui sont responsables pour la disparité actuelle de ce paysage. Partant de l’hypothèse que non seulement les conditions socio-économiques mais aussi les conditions naturelles sont beaucoup plus favorables dans le piémont que sur le plateau, nous avons - par une approche interdisciplinaire - effectué une analyse et une évaluation de ces facteurs. Des méthodes de la télédétection, de la pédologie, de la botanique et de la géographie agraire ont été employées.
The vast distribution of terraces in the geographical Sudan zone of West Africa leads to the question why and under which conditions an agrarian society might apply this particular form of farming. From an anthropologist's point of view it is essential to understand why farmers practise this form of farming and therefore try to explain the reason for it. The best way to gain insight is the description of terrace farming and when taking a closer look, we realize that farming is nowhere only an isolated agricultural activity.
L’inventaire floristique du Burkina Faso est loin d’être clos. Les récentes investigations floristiques ont permis de recenser un grand nombre d’espèces qui n’avaient jamais été mentionnées sur la liste floristique nationale. Une partie de ces espèces sont d’ailleurs guinéocongolaises. Cet inventaire a concerné les aires classées et protégées situées dans le secteur Sudsoudanien. Les richesses floristiques se retrouvent concentrées dans ces aires classées et protégées car ce sont des formations qui jouissent d’une relative bonne gestion par rapport aux autres aires non protégées qui ont été fortement dégradées par l’homme. L’objectif de cette communication est de donner les informations issues de ces différentes investigations sur l’état de connaissance de la diversité végétale du Burkina Faso avec un accent particulier sur le mode de gestion et les utilisations de quelques espèces de la zone sud soudanienne.
L'art de soigner par les plantes ou phytothérapie remonte à l'origine des temps. L'Homme a développé trés tôt une relation intime entre lui et le milieu qui l'entoure. Pour subvenir à ses besoins, il a appris à se servir des éléments naturels indispensables à son existence. Si en Europe la phytothérapie, jadis florissante, a connu un déclin à cause de la découverte de molécules chimiques de synthèse, en Afrique les médicaments à base de plantes restent les produits les plus utilisés par l'immense majorité de la population dans des villages comme des villes. C'est le cas du Burkina Faso où la pharmacopée traditionnelle, en temps que système de soins, demeure le système le plus populaire parce que mieux intégré aux réalités socio-culturelles des populations. Pour notre exposé, nous avons retenu dix espèces de plantes qui sont utilisées dans la médecine traditionelle populaire pour traiter les maladies les plus courantes comme les accés fébriles dus au paludisme, la jaunisse ou ictère, les diarrhées, la toux etc.
Our dichotomy of ‘nature’ and ‘culture’ is expressed in the Kanuri language with the terms al@ga for ‘creation’ and ‘creature’ which embraces trees, mammals, birds, insects, humans, in short the whole of the natural environment, and ada for ‘custom, habit, way of behaviour, family tradition’ for culture as a whole. There is no genre of oral literature, which would describe al@ga as such, but aspects of it can always be expressed in proverbs, riddles, toponymic praise phrases and songs, of which those performed by the hunters figure most prominently in reflecting upon al@ga. Yet, in these songs (and partly in other genres) ideas about al@ga are not purely descriptive in naturalists’ terms. They are much rather expressions, which centrally combine notions of the social and natural environment.
In the culture of the Pero, Longuda and Tula People in the south-eastern part of Bauchi State, north-eastern Nigeria, terraces are found as traditional means to improve the environmental condition and to secure the survival of the people. To classify those terraces according to their form and function, the techniques and customs of their building and the traditional structures of their development they have to be compared in the context of their own culture. The paper gives a few examples showing that the importance of terraces for the historic and religious concepts of the Pero, Longuda and Tula People is expressed through a tight network of oral traditions, social and religious customs and structures of belief and explanation, which, once they were woven together, eventually build what a malam from Tula called a glue of inheritance, identity, integrity, continuity and security.
This paper, which is based on field research in Doron Baga and Maiduguri, attempts to describe and analyse the main determinants of the fish trade in North-east Nigeria with Doron Baga as the main focus. Fish is a significant source of income and a principal trade commodity, bringing together the remote fishing community of North-east Nigeria with the major urban centres of the Southern part of the country. In order to get an insight into the workings of the fish trade, indepth interviews were conducted with fatoma (or dealers), traders and transporters in Doron Baga and Maiduguri. In addition, the authors utilised documentary sources to supplement the data collected from the field. For instance, a number of postgraduate dissertations, archival accounts as well as journal articles and books were consulted. After giving a brief introduction of the development of the long distance trade in fish, the paper discusses the role of the fatoma, means of transportation, value and volume of the trade. The study focuses on the period 1997-1999, during which substantial part of the data used were collected.
The architecture and chemistry of a dug-out: the Dufuna Canoe in ethno-archaeological perspective
(1996)
It is the intention of this paper to highlight the processes involved in the production of a dug-out. Two disciplines appear strikingly clear in the title of this paper; architecture and chemistry. It is deliberate, exhibiting the multifaceted approach to issues in archaeology. The Dufuna canoe, the main subject of the discussion, is entirely an organic material, long used by prehistoric populations, abandoned and covered in a huge deposit of earth, unearthed by the spade in two streams of excavations for the purpose of dating, measurements, documentation, which yielded a date of 8500 years as the oldest canoe in Africa and one of the oldest in the world. Who could have produced such an "artefact"? These and other related questions are fundamental towards the understanding of the history and society that lived in that environment in prehistory. Since we are dealing with a single "artefact" produced by prehistoric populations, long gone and extinct, we would not be in a position to reconstruct the processes of manufacture of the dug-out by any source other than by ethno-archaeological and ethnographic investigation and experiment of the contemporary society which manipulates similar environment with a view to stimulating the past mode of production. The method used in the data collection was by oral interviews and field observation.
The architecture and chemistry of a dug-out : the Dufuna canoe in ethno-archaeological perspective
(1993)
This research work emanated from a joint research project between the Johann-Wolfgang Goethe-University Frankfurt am Main, Germany and the University of Maiduguri, Nigeria; as outlined in the Bilateral Agreement of July 21st 1988.1 The research program is interdisciplinary in nature involving these areas: Archaeology, Geography, Linguistics and Historical Ethnology; all under the general theme of West African Savannah. Considerable research work has already been carried out in these areas by German explorers and scholars dating back to the mid-nineteenth century. The project is funded by the German Research Foundation. The present paper addresses itself purely to one and very important aspect of an archaeological campaign undertaken in the Chad Region of Nigeria in late 1990/1991 season. In consequence to reconnaissance survey and excavation conducted at a site called Gajiganna, abundant lithic materials were noticed and collected. The crucial question one poses to the site which lacks physical outcrops in and within the precinct of the settlement are, what could have been the source of the raw materials at the site? Were they transported from somewhere to the site? If so, why was it necessary for the materials to be brought to this site? These and other related questions posed a serious commodity problem for most sites in Borno with lithic materials.
Summary and evaluation
(2000)
The geographical analysis of the Savannah Zones of north-eastern Nigeria revealed a basic insight on the differentiated development at the meso-regional level: The northern districts had a much lower population increase than the average. It was already arid land before the dramatic reduction of precipitation since the late 1960s. The quality of the soil for farming is rather poor despite specific minerals which give grass during the very short growing season a higher nutritional value than further south. Through studies at the local level, it became evident that on one side this is an area of out-migration of the local population but on the other side we have to register the influx of migrants from the even more dryer northern districts including Niger Republic. The rate of urbanisation in Nigeria increased rapidly from 15 to 36 %. Northern capitals like Kano and Maiduguri multiplied their number of inhabitants seven times but were overtaken by Gombe’s growth. In any case they are centres of social and cultural change as the preliminary findings of H. BALZEREK concerning the boom-town Gombe revealed. But social tensions seem to be inevitable as religious movements not only in big towns like Kano but even in Gombe were already analysed by WATTS (1993, 61). The bearer/ carriers of this movement are landless people who did not migrate to new land in the country side, they are hoping for new jobs in an urban environment. The social structure has changed since the time of the old Hausa towns, but their reliance on surplus of labour force appears to have continued.
The search for persistent elements in nature and culture, which comprises language as a constitutive part, is a prerequisite for the definition of any change which is the aim of our common project. When analysing the process of transformation by a number of disciplines we expect to discover significant features of this alteration and the forces dominating it. The current highly complex present linguistic situation in the western and south-western fringe of the Chad Basin will be reconstructed from the historical migrations undertaken by the various linguistic groups from Lake Chad (mainly Chadic languages) to their present settlements. The six authors, the linguists Dr. Dimitr Ibriszimov, Dr. Doris Löhr, Christopher Mtaku, the ethno-musicologist Dr. Raimund Vogels, and the historian Ibrahim Maina Waziri integrated the results of their studies into one paper towards a systemic approach by tracing back the common roots of the languages, the customs and the music of those peoples and give an outline of their tradition concerning their movements. A basic dual model of migration will be put forward.
In Nigeria terrace agriculture can mainly be found in the so called "Middle Belt Economy" as FORDE (1946)1 coined this type which lies between the grain economy of males in the north and tuber cultivation of females in the south. The people - lacking a hierarchically social and territorial organisation - are called acephalous or segmentary societies. From the geographical point of view the Middle Belt is seen as a zone of transition. Because of the variability of the climate (sometimes it is too wet for grains, sometimes too dry for tubers) a strategy of mixed cropping enables the farmers to overcome these hazards. Their strategy can be seen in the frame of the game theory. A low population density and a lack of sufficient accessibility limited the innovation of cash crops at that time. The papers on the Tangale-Waja Region will reveal manifold facets of the culture and agriculture. In a first step we learn by the research of J. Heinrich that the natural environment is - from the genetic point of view - a prerequisite for the establishing of terraces, but it is still today an important provision to the modern farmers in their resettlement areas.
Les Kassena, une ethnie sédentaire appartenant au groupe linguistique des Gurunsi, habitent dans une région au sud du Burkina Faso et au nord du Ghana. Leur économie est basée sur l’autosubsistance à partir de la culture du mil et l’élevage du bétail. Avec une saison des pluies de six mois et une pluviométrie de plus de 900 mm, la région offre des conditions favorables à la culture du mil. Cette région est caractérisée par une forte inégalité en ce qui concerne la dispersion de l’habitat. Certaines régions, comme les alentours de la montagne de Tiébélé qui, selon les récits de la tradition orale, est l’ancien centre des Kassena de l’est, connaissent une importante population allant jusqu’à 100 habitants par km/carré. D’autres régions, notamment la vallée du Nazinon, étaient plus ou moins inhabitées jusqu’à ces dernières années. Ceci est probablement dû aux maladies endémiques comme l’onchocercose. Pour les régions les plus peuplées, nous avons cherché à connaître les techniques spécifiques qui ont permis à la population de s’installer et de s’alimenter. Les Kassena maîtrisent un système de culture permanente sur des champs terrassés exigeant beaucoup d’entretien. Ces champs sont soumis à un contrôle social et religieux. De plus, les Kassena cultivent des champs de brousse qui se trouvent souvent dans la plaine et à grande distance des villages. Ces champs de brousse sont cultivés d’une façon plus extensive. Le présent exposé décrit les techniques de l’agriculture en respect des conditions de l’environnement. L’objectif est de mieux comprendre les stratégies économiques et culturelles des cultivateurs de cette région.
L’Afrique, de par la diversité de ses écosystèmes, de la richesse de ses ressources et de par la confrontation de deux conceptions d’aménagement, l’une africaine l’autre externe, offre à la télédétection satellitaire l’un des meilleurs champs d’application. Cependant, une conscience claire des besoins spécifiques en informations par rapport aux objectifs à atteindre manque. Ceci est dû à l’insuffisance d’une politique de développement cohérent, dont la dimension spatiale n’est rien d’autre que l’aménagement du territoire. Tel est le sens de ce travail qui offre deux études de cas: Compiéna dans le département de Pama, province du Gourma et Leo dans le département de Léo province de la Sissili. L’exploitation des données satellitaires concernant les zones d’étude Compiéna et de Léo s’est faite en l’absence ou insuffisance de certaines données de base nécessaires. Cependant, elle complète ces dernières ou pallie en partie à ces défauts, permettant ainsi une bonne connaissance de la réalité spatiale du terrain. Ceci a conduit à l’objectif recherché, consistant à évaluer les besoins en informations pour l’aménagement de Compiéna et de Léo.
Our paper deals with the problems of migration, culture and language in the wider Benue-Gongola basin. Here are mainly concerned the West-Chadic speaking groups Kwami, Kupto, Kushi and Piya as well as the Jukun who speak a language belonging to the Benue-Congo family. We try to point out the possible reasons for their historical migrations and in particular the consequences of ethnic expansion of the Jukun in the middle Benue region. History shows that contacts of ethnic groups - being peaceful or by force - had always led to mutual influences and changes in culture and language, which finally resulted in cultural fusion of various aspects. Our study, based mainly on oral traditions as well as on linguistic comparisons, focusses especially on the history of the above mentioned Chadic groups, who are considered - according to our hypothesis - to have come in close contact with the Jukun. Subsequently the warlike expansion of the Jukun caused a strong turmoil which led to the scattering of the various ethnic units.
La langue mandingue est un vaste continuum linguistique recouvrant une zone de l'Afrique Occidentale qui s’étend de l’embouchure de la Gambie à l’Ouest à la frontière occidentale du Ghana à l’Est. Si actuellement on dispose de nombreux travaux sur le bambara, le dioula et le mandinka qui ont permis de mieux connaître le mandingue, il n’en est pas de même en ce qui concerne cette autre variété du mandingue: le marka du Burkina Faso pour lequel des études linguistiques sont pour l’instant rarissimes. L’essentiel des travaux qui lui sont consacrés sont présentés dans la bibliographie. Notre objectif ici est de mettre en évidence quelques traits spécifiques au marka par rapport à d’autres dialectes mandingues sur le plan phonique, tonologique et grammatical. Sur le plan grammatical nous présenterons quelques faits qui rapprochent le marka et le mandinkan de Gambie. Mais avant d’y arriver nous allons procéder à une présentation du marka.
Dans les sociétés peules, des migrations de toutes dimensions jouent traditionnellement un rôle très important. Elles sont le résultat d’un processus complexe de prise de décision. Notre contribution vise à fournir des informations sur les motifs qui influencent ces décisions, et à partir de quels concepts ces motifs sont générés. Un élément central dans ce processus est l’environnement. S’il y a quelque peu un environnement ou une culture peule homogène, il n’y a cependant pas de conception peule de l’environnement. Leur conception est modifiée lors de chaque intégration des Peuls dans un nouvel environnement. Migrer signifie se rendre dans un nouvel environnement, où les concepts existants doivent par conséquent être adaptés aux nouvelles conditions. Par la suite, nous allons montrer à l’exemple des Peuls agropastoraux de différents milieux naturels et sociaux du Burkina Faso, Bénin et Nigeria dans quelle mesure les connaissances de l’environnement peuvent influencer les décisions migratoires. A l’exemple de la végétation, on examinera à travers quels concepts cet environnement, plus précisément l’environnement physique, est classifié et évalué. Finalement il sera présenté comment les migrations influencent les concepts environnementaux et comment ceux-là se reflètent dans la langue.
Les concessions chez les Bisa, dans la Province du Boulgou (sud-est du Burkina Faso), sont des exemples représentatifs de l’architecture traditionnelle soudanienne: un rassemblement de cases rondes de glaise d’un diamètre d’environ 3 mètres, couvert d’un toit de paille. Les concessions sont entourées par un mur de glaise et on ne peut y entrer ou en sortir que par une seule entrée. On ne découvre leur complexité que seulement après y être entré. C’est seulement à ce moment-là que l’on peut voir le dédale de cases et de cours, séparés par de petits murs et des couloirs. La concession du chef de Bangagou, chez qui nous avons habité durant l’automne 1994 pendant quelques mois, compte parmi les plus grandes de la région. Pendant les premières semaines de notre séjour, il nous a fallu comprendre son histoire et sa structure. Notre premier objectif était de dessiner un plan de la concession. Ce projet s’est révélé plus difficile que prévu. Le terrain étant si plat, nous n’avons pu voir la concession d’en haut. L’achèvement de ce plan n’a été possible que parce que les habitants nous ont donné libre-accès à ces lieux. La vérification de l’esquisse fut possible en décembre, lorsque nous avons eu la chance de faire un vol au-dessus du terrain et de prendre des photos aériennes. Parallèlement, nous avons mené une enquête auprès des habitants afin de connaître leurs relations avec les habitants des autres cases, selon leurs descriptions.
The area around the Lake Chad is characterized as an example for a region where ethnic changes abundantly took place and still do. For example some Kanuri districts, or the leaders of those districts, are (unofficially) named after other ethnic names (e.g. Margi, Shuwa) or Kanuri clan names are identical with ethnic names of other groups, eg. Tera, Bade. Both people speak a Chadic language and live in the south and west of the Kanuri respectively. These are indications that the Kanuri formerly absorbed and integrated these peoples. These processes are not only a phenomenon of the past. In the case of the neighbouring Gamergu people an ongoing process of ethnic change towards a Kanuri identity is observed until present. The research projects1 have revealed that the concept of "ethnic units" is far from being static which the term may suggest. This especially applies to the German Stamm, which implies a static concept of ethnicity. However, in Borno the dynamics of ethnic and linguistic change are prevailing. Therefore Ronald Cohen rejected the term "ethnic unit", or even "tribe" for the Kanuri and preferred "nation" instead. Umara Bulakarima argued along the same line but used "ethnic group" for Kanuri subunits, e.g. Manga, Mowar, Suwurti. There is no doubt that the Kanuri played a dominant part in the history of the Lake Chad area during the past centuries. Therefore the "Kanurization" process may not surprise. However, in the following it will be revealed that the processes of contact and resulting adaptations and delimitations are not necessarily unidirectional from Kanuri to other groups. At least in some cases they may go into the opposite direction, e.g. from Gamergu to Kanuri.
Le Burkina Faso connaît une urbanisation relativement modérée. En effet, selon le recensement de décembre 1985, le taux d'urbanisation s'élevait à 13,6% et cela en tenant compte des seize centres secondaires de plus de 10.000 habitants. En se basant sur les 119 localités de plus de 5.000 habitants, ce taux dépasse à peine 27%. Or des pays tels que la Côte d'Ivoire, le Ghana et le Sénégal présentaient au même moment des taux de 45 à 50%. Cependant la taille et le nombre des centres urbains ne cessent de croître. Mais jusqu'à présent, le pays ne compte que deux principales villes: Ouagadougou et Bobo Dioulasso avec respectivement 441.514 et 228.668 habitants soit 70% environ de la population urbaine nationale. Les estimations de 1992 donnent à Ouagadougou 842.000 habitants et environ 400.000 à Bobo-Dioulasso. Ouagadougou, la capitale est sans doute le pôle le plus développé avec une armature urbaine assez complète, des activités et des fonctions diversifiées. Le développement urbain est ici plus perceptible qu'ailleurs, il en est autant des problèmes: équipements insuffisants et inadaptés, marginalisation des populations démunies, destruction du patrimoine naturel, forte croissance démographique. Les problèmes environnementaux sont multiples et se manifestent différemment avec plus ou moins d'acuité. C'est cet aspect précis que nous allons analyser.
Les Bisa et les Samo, séparés d’une distance de 300 kms (distance en ligne droite), ne sont pas seulement liés par leur appartenance au monde mandingue, plus une forte parenté de leurs langues, mais aussi par des ressemblances de leurs structures politiques. Tous les deux ethnies appartiennent ni aux groupes étatiques, ni aux groupes segmentaires, mais elles font partie d’un groupe intermédiaire: elles ont, comme caractéristique, une chefferie traditionnelle avec des tendances à une hiérarchisation et à une existence de cantons précoloniaux. En plus, ces deux groupes ont en commun qu’il y a des variations du système politique dans la région qu’ils habitent. Dans la première partie de cet article il est envisagé de faire une esquisse des systèmes politiques dites « traditionnels » des Bisa et des Samo sous l’égard des influences externes: précoloniales, surtout par les Mosi et des Marka, coloniales et postcoloniales. La deuxième partie sera consacrée aux processus actuels et aux perspectives d’avenir: les deux régions sont, présentement, concernées par des immigrations et interventions massives, causées par des actions d’Etat. Ces développements qui peuvent être observés dans la région samo depuis 30 ans, sont, dans la région bisa, seulement à leur début. Cela nous permet de faire une analyse pour la région samo au Sourou et une tentative à donner une perspective pour la région des Bisa.
The paper takes recourse to oral tradition and linguistics to ascertain the assertion that the presentday Kanuri and Kanembu speech forms emerged from the same parent language. In determining the parent language, the descriptions of the various components (i.e. clans and ethnic groups) of Kanuri and Kanembu are given as a first basis and the relation of each dialect of Kanuri and Kanembu to the other (i.e. dialect contiguity) is demonstrated as a second basis. Taking into consideration the sociolinguistic background of both Kanuri and Kanembu, the brief history of their divergence, the strong contention of the Borno Ulama and the dialect contiguity of the data presented, the paper concludes that Kanuri and Kanembu are initially one and the same language with ancient classical Kanembu being the parent language.
Zum Landnutzungswandel in der südlichen Sudanzone am Beispiel des Bauchi State (Nordost-Nigeria)
(1995)
In der südlichen Sudanzone Westafrikas sind die Aktivitäten des wirtschaftenden Menschen seit langer Zeit die Hauptfaktoren der Landschafts- und Vegetationsgestaltung. Die ursprüngliche natürliche Vegetationsdecke - entsprechend der klimatischen Gegebenheiten wahrscheinlich laubabwerfende Trockenwälder - ist durch anthropogene Eingriffe in vielfältiger Weise verändert oder auch gänzlich beseitigt worden. Die Veränderungen bestehen einerseits aus direkten Eingriffen durch Rodung (für Siedlungs- und Anbauflächen), die selektive Nutzung von Pflanzen (Brennholzeinschlag und Holzkohleherstellung, Bauholznutzung, Beweidung, Laubschneiteln und Sammeltätigkeiten) und durch gelegte Buschfeuer (Aufspüren von Jagdwild, Stimulanz neuen Graswachstums und "Öffnen" der Pflanzendecke vor Unterkulturnahme). Andererseits haben die anthropogenen Einwirkungen je nach Art, Intensität und Dauer auch die natürlichen Standortbedingungen (vor allem Boden, Wasserhaushalt und Klima) verändert. Nicht zu vernachlässigen sind des Weiteren die nachhaltigen Eingriffe in den Wildtierbestand. In weiten Teilen Westafrikas wurde der am Anfang des 20. Jahrhundert noch recht arten- und individuenreichen Wildtierbestand fast vollständig verdrängt oder ausgerottet. Im vorliegenden Beitrag sollen am Beispiel des südöstlichen Bauchi State die Veränderungen der allgemeinen sozioökonomischen Rahmenbedingungen und die wichtigsten Maßnahmen zur Entwicklung der Land- und Forstwirtschaft und der dadurch bedingte Landnutzungswandel skizziert werden. Ausgangspunkt ist dabei der Zustand der Landschaft zu Beginn der Kolonialzeit, die hier in der ersten Dekade des 20. Jahrhunderts begann.
Toponyme als Zeugen der Vergangenheit : Untersuchungen im nordnigerianischen Bergland der Hausa
(1995)
Toponyme können aufmerksamen Beobachtern wertvolle Hinweise auf Lebensweise und auch auf Identität oder Herkunft von Bewohnern bzw. früheren Siedlern einzelner Landstriche geben. Beispiele hierfür sind die Bezeichnungen für Naturerscheinungen wie Landschaftsformen, Flüsse bzw. Flußbetten und Gebirge, aber auch für vom Menschen geschaffene Gegebenheiten wie Siedlungen, Anbauflächen oder zu anderen Zwecken veränderter und damit genutzter Naturraum. Toponyme lassen sich jedoch nicht immer leicht entschlüsseln. Oftmals ist ein Grundwissen zur Geschichte und Lebensweise der Menschen, in deren Umfeld die Namen gebraucht werden, unerläßlich, um auf Besonderheiten überhaupt aufmerksam werden zu können. Dieser Beitrag beschäftigt sich mit der Bezeichnung von Bergen, einzelnen Steinen, von Steinformationen und vom Menschen angebrachten Veränderungen an Steinen und Felsen. Als Untersuchungsraum dienten die ausschließlich von Hausa besiedelten Gebiete nördlich der Provinzhauptstadt Dutse (Jigawa-State) sowie die Region südlich von Gano, die rund 40 km südöstlich von Kano in Nordnigeria liegt.
Wasserfahrzeuge sind die ersten Verkehrsmittel der Menschheit. Es gibt Überlegungen, ob sie eine zentrale Rolle beim Vordringen der neolithischen Lebensweise nach Mitteleuropa gespielt haben, aber auch zwingende Annahmen, dass sie bereits in weitaus früherer Zeit zur globalen Verbreitung des Menschen beigetragen haben. Im Rahmen des Frankfurter Sonderforschungsbereichs "Westafrikanische Savanne" wurden im nigerianischen Teil des Tschadbeckens Untersuchungen an einem Boot vorgenommen, dessen Alter eine neue Sicht über die Beteiligung Afrikas an der Geschichte der Verkehrsmittel erfordert. Das im Folgenden vorgestellte Boot stammt aus einer Zeit, die im Tschadbecken archäologisch nur außerhalb der damaligen Erstreckung des Mega-Tschads faßbar ist und die der Erfindung der ältesten Fortbewegungsmittel der Menschheit einen originär afrikanischen Ursprung verleiht.
Probleme der Nutzung von Weideressourcen bei den Shuwa-Arabern im nigerianischen Tschadbecken
(1995)
Die Weidewirtschaft, wie die Shuwa-Araber des Tschadsee-Gebietes sie betreiben, ist ein Forschungsbereich, in dem sich die Wechselbeziehungen zwischen Mensch und Umwelt in einem Savannen-Habitat besonders deutlich nachvollziehen lassen. Die Weidepflanzen sind von Klima- und Bodenverhältnissen und die Herdentiere in einem nicht unerheblichen Maße von geomedizinischen Faktoren wie der Verbreitung der Tsetse-Fliege abhängig. Der Mensch beeinflußt durch sein wirtschaftliches Management, die Auswahl und Zucht seiner Haustiere, die Frequentierung von Weidearealen und Transhumanzrouten, künstlich angelegte Buschfeuer, Anlage von Forstreserven etc., die naturräumlichen Gegebenheiten. Er wirkt mit diesen Eingriffen auch auf Selektionsmechanismen in der Pflanzenwelt hin und gestaltet die Savanne gewissermaßen zu einer "Kulturlandschaft" um. Die Savanne Westafrikas dient zu einem Großteil als Weideland, und bei dieser Nutzungsart tritt die Interdependenz zwischen dem Menschen und seiner "natürlichen" Umwelt besonders deutlich hervor. Es ergibt sich daraus eine Vielzahl von Fragestellungen, die hier zumeist nur anzusprechen, aber nicht erschöpfend zu beantworten sind. Einige der Grundfragen für diese Thematik können wie folgt formuliert werden: - Wie "natürlich" ist der als Weideland dienende Savannenraum? - Mit welchen naturgegebenen Determinanten werden die Tierhalter konfrontiert? - Über welche kultur- bzw. ethnospezifischen "Pläne" verfügen sie, um sich den Bedingungen ihrer ökologischen Nische anzupassen? - Trägt die viehwirtschaftliche Produktion eher zur Zerstörung oder zur Bewahrung der natürlichen Ressourcen bei?
Studies on land use in Africa have usually been carried out by ethnologists or human geographers and were rarely concerned with data on the physical conditions of soil. There is hardly any issue, however, where interdependencies between natural and cultural factors are as evident as in the topic of land use. For this project the approach of three ethnologists, Braukämper, Kirscht and Platte, was therefore combined with the analysis of Thiemeyer as physical geographer. The area of research is the Local Government Area of Marte in the Nigerian State of Borno. As part of the Chad Basin this region is mainly characterised by clay sediments which are commonly labelled firgi by its inhabitants. Beside this general term, however, the local peasants clearly distinguish five types of soil (Kanuri: katti), to which different physical conditions and qualities with respect to their cultivation are attributed. The question arose how far can this popular knowledge, accumulated by agricultural experiences over generations, be correlated with scientific data. That is why samples of the mentioned types of soil were collected by the members of our team and analysed in the laboratory of the Frankfurt Institute of Physical Geography. The detailed presentation of this analysis has to be preceded by the classification of the respective soil types in the terminology of the indigenous farmers.
En se concentrant sur le thème „Migration rurale et gestion des ressources naturelles“, cette section du symposium à tiré l’attention sur un aspect très important concernant le changement de l’espace naturel et culturel dans l’air rural de Burkina Faso. A partir de cas régionaux différents au Burkina Faso, les études pluridisciplinaires se concentrent sur des aspects centraux du complexe migratoire: • les types d’appropriation de terre • l’impact de la coexistence des droits fonciers traditionnels et modernes • la dégradation des ressources naturelles
With one group generally constituting the autochthonous host - representing the core population in the centre - immigrant groups tend to reside in separate ethnic wards and even work in wards/quartiers identified with their ethno-specific crafts and trades - and often named after them. The socio-lingustic survey will therefore use available and new maps and ethno-linguistic statistics: For the former, the urban surveys by the Max Lock Company of north-eastern Nigeria have been of great help, but have to be updated ; for the latter, various censuses had to be supplemented by more recent information . With ethno-linguistic wards constituting enclaves which can only interact through a language or languages in common, we can apply the general model of the triglottic configuration by positing x territorial and y immigrant, ethnic languages of solidarity; one general urban community language or lingua franca of interaction; and the official language of authority and administration. This language of authority was formerly a local aristolect (Kanuri or Fulfulde), but is now mostly an exolect - English or French. This short presentation concerns ongoing work in urban socio-lingustics developed in Maiduguri over some 15 years.
Changes in settlement pattern and culture - the process of down-hill migration in Tula, Bauchi State
(1996)
The process of down-hill migration of the Tula people started during the 1920s and has not yet finished. The resulting present situation might give information how far terraces play any role in the economy, ecology and ideology of the Tula. Approaching this question from a socio-agricultural point of view some facts which indicate the pertaining or overcoming of traditional structures will be presented. In the following the land tenure system, the adoption of innovations and the role of women in agriculture will be discussed comparatively for Tula Wange and Tula Baule on the plateau, Fantami, which is generated by down-hill dwellers of Tula Wange, with its more or less bad farming conditions on shallow sandy soils and Kaltin, where the down migrants of Tula Baule settle in a more fertile area. Tula Wange numbers around 2000 households, Baule 1000, Fantami about 200 and Kaltin 350 of which the sample survey includes 15% in the plateau sites and 25% in the plain settlements.
Notre communication porte sur le thème suivant: "Anthropisation du couvert végétal dans la province de Namentenga et ses conséquences socioéconomiques: cas de la région de Tougouri, en zone subsahélienne" (centre-nord du Burkina Faso). A travers cette étude de cas, nous voulons attirer l'attention sur les problèmes environnementaux du Burkina Faso. En effet, les déficits pluviométriques cumulés, depuis quelques décennies, sont à l'origine de la dégradation des écosystèmes. La surexploitation des terres entraîne également l'épuisement des sols et la diminution des ressources végétales. Un telle situation n'est pas sans conséquence sur les activités socioéconomiques des populations. Le plan de notre communication sera donc comme suit: 1. Présentation du degré-carré de Tougouri, sur le plan physique 2. Dynamique du couvert végétal sous l'action anthropique, et les conséquences socioéconomiques 3. Perspectives d'avenir
La coupure que l'on constate dans les forêts denses africaines au niveau du Togo et du Bénin soulève de nombreuses questions sur les rapports forêt/savane au cours des derniers millénaires. Le projet Dahomey Gap vise à y répondre, par une approche pluridisciplinaire intégrant botanique et histoire de la végétation dans le Sud-Bénin et le Sud-Ouest du Nigéria. A partir de travaux déjà avancés sur la végétation du Bénin et des premières prospections palynologiques, quelques aspects du projet sont exposés ici: tels la caractérisation des différentes formations forestières du Bénin, les premiers résultats sur l'histoire holocène de la végétation du Sud-Bénin (mangroves, forêts, savanes) enregistrée depuis près de 7000 ans dans les lagunes.
In a previous study which originally tackled the apparent contradiction between oral tradition and linguistic evidence in the Babur-Bura case, we approached the issue through a contrastive analysis of Bura and Kanuri. Since the originstory tends to push the Babur towards the Kanuri, leaving the Bura to stand all alone, it was felt that any linguistic closeness between Kanuri and Babur would confirm the originstory. Unfortunately, the paper did not come up with such evidence. The paper in question summarizes COHEN's (1983) account and interpretation of both the Babur and the Bura versions of their origin. It then presents the loopholes in the various accounts, based not only on the current linguistic classification of the area, but also on the results of an investigation carried out within the framework of the "Borno Surname Project". At both the phonological and syntactic levels, BADEJO (1989) observes that in view of certain fundamental differences between Kanuri on the one hand and Babur-Bura on the other, Babur affiliation with Kanuri is doubtful. Such differences include: the lack of voice distinction between the labiodental fricatives in Kanuri (i.e. /f/ and /v/; the /p/ - /f/ alternation), especially in wordinitial position in Kanuri, and finally, the SOV structure of the Kanuri sentence. The paper, drawing on support from an opinion survey, therefore concludes that "general linguistic and the social linguistic considerations presented ... seem to point to the fact that the Babur and the Bura are, by and large, the same people". The paper, however, recognizes the need for a Babur-Bura contrastive study. The current paper is the first step in that direction.
Le SIG-BF est destiné comme outil pour la visualisation et l’analyse des informations spatiales pour les scientifiques du «SFB 268 – Histoire des cultures et des langues dans l’espace naturel de la Savane Africaine» à l‘Institut Géographie Physique de la Johann Wolfgang Goethe-Universität à Frankfurt am Main et leurs collèges de l’Université de Ouagadougou. En outre, les données peuvent être utilisées par d'autres dans un but scientifique. La copie et la diffusion des données sont permises si la source est clairement mentionnée. L’usage professionnel et le traitement des données du SIG-BF dans un but commercial, soit sous forme imprimée ou sous forme digitale, nécessite la permission écrite de l’Institut Géographie Physique de l’Université Francfort. Les données spatiales qui sont assorties dans le BF-SIG sont basées sur plusieurs sources. En général, il y sont groupées des informations de l’environnement naturel et anthropique à l’échelle nationale de Burkina Faso. En majorité ce sont les données des cartes topographiques et thématiques du pays et quelques données statistiques. La connaissance des paramètres (p. ex. échelle, age, projection) des sources des données est une condition importante pour l'évaluation du degrée d'exactitude des résultats des analyses SIG. Quant à ca, quelques notes importantes sont démontrées dans la 2ième chapitre de ce texte. Les sources indiquées dans la table de matières (3ième chapitre) sous la forme des ‘numéro de la source’ sont précisées dans la 4ième chapitre.
Au nord du Burkina Faso aucune autre époque culturelle, à l'exception des temps modernes, n'a laissé autant de traces sur une aussi grande surface que l’Age de Fer. L'étude de cette période joue alors un rôle important dans la reconstruction de l'histoire culturelle du Sahel. D’après les dates obtenues dans nos fouilles différentes, l’Age de Fer s'étend de la fin du premier millénaire BC jusqu'au milieu du 14e siècle AD. Les recherches du projet multidisciplinaire G4 du SFB 268 visaient les questions suivantes: Quelle est l’apparence physique des collines d’occupation et quelle est leur répartition dans la région ? En quoi consiste la culture matérielle trouvée sur les sites et qu’est-ce qu’elle permet de dire sur la vie quotidienne? Comment l’interaction entre l'homme et l'environnement se présentaitelle à l’époque et quelles étaient les ressources naturelles exploitées? Quelle population occupait les buttes, et quels indices l’étude de l’histoire du peuplement peut fournir comme causes de leur abandon final? Le vaste champs d’études ouvert par ces questions impose une approche multidisciplinaire. Les résultats qui seront présentés cidessous n'ont pu être obtenus qu'en coopération de l'archéologie, l'archéobotanique, l'archéozoologie, l'histoire et la géographie.
In a recent article dealing with the Tangale Peak or Kilang, as it is called in the local Tangale language, Herrmann JUNGRAITHMAYR presents an account narrated by a Tangale elder about the attempted ascent of that characteristic mountain by a British colonial officer and his subsequent death.1 Kilang mountain is a basaltic cone approximately 1300 m high, about 8 km southwest of Kaltungo, one of the principal settlements of the Tangale people, in southern Bauchi State, northeastern Nigeria. During a research stay at the National Archives in Kaduna in November 1993 I was able to consult a file containing various documents relating to this incident in detail.2 In the following note I present an outline of the events based on the evidence in the colonial records. By doing this I not only intend to shed more light on a tragic event from the very early years of the colonial era. The picture of the circumstances emerging from the investigations of the colonial authorities may serve as a background to the narrative by the Tangale elder presented in JUNGRAITHMAYR's publication.
The craggy and hilly Muri Mountains, which are situated to the north of the Benue Lowlands, are an area with a complex pattern of settlement. This roughly 80 km long and 20 km wide mountainous area is inhabited by about 20 ethnic groups belonging to different language families. The present ethnic and linguistic situation is understood as the result of a complex series of migrations and adaptations to the natural environment. This paper will describe actual movements of settlements and consider certain conditions which may have been relevant in the decision to leave a settlement or choose a new one. The most important conditions will be the accessibility of arable land and/or pasture, accessibility of water, and conditions dependent on the historical and political context such as affording of security and possibility of defence. Therefore an interdisciplinary approach seems to be appropriate to evaluate the natural conditions for settlement and cultivation of the various places from a geographer´s point of view, to interrogate into the historical aspects and motifs of the settlement patterns and migrations with a thorough ethnological background, as well as to gain additional information from a linguistic analysis of toponymes and contact phenomena of the languages spoken in the area.
Population structure was estimated in a continuous population of a small land snail (Trochoidea geyeri). Mark-recapture experiments and randomly amplified polymorphic DNA analyses indicate that the population structure can be described by the isolation by distance model of Wright (1946). Estimates of density and dispersal suggest a neighbourhood size of 70-208 individuals on an area of 13-21 m². A principal component analysis of the randomly amplified polymorphic DNA data reveals clinal variation of genetic composition across the population, as predicted by the neighbourhood concept. An analysis of molecular variance indicates substantial genetic structuring. Comparisons of the genetic distances, expressed as euclidean distances among individuals, versus the geographic distance between sampling sites yield a highly significant positive correlation (Mantel test: r = 0.567, p<0.0001). The revealed pattern of populational subdivision on a microgeographic scale seems to be one of the principal processes generating and maintaining genetic diversity within populations of small land gastropods.
Das nachfolgende, so viel bis jezt bekannt, älteste Verzeichniss eines großen Theiles der Tafelgüter, Einkünfte und Rechte eines Erzbischofs von Mainz, und anderer auf die Erhebung und Verwaltung derselben bezüglicher Verhältnisse, war aus dem ehemaligen Kur-Mainzischen Archive, mit den die Stadt Erfurth betreffenden Akten, in das Königliche Provinzial-Archiv zu Magdeburg übergegangen. ...
In the present study the population genetic structure of the terrestrial snail Pomatias elegans was related to habitat structure on a microspatial scale. The genetic variability of 1607 individuals from 51 sampling sites in five different populations in Provence, France, was studied with an allozyme marker using population genetic methods, Mantel tests and spatial autocorrelation techniques were applied to different connectivity networks accounting for the structural features of the landscape. It is suggested that the population structure is, to a large extent, a function of the habitat quality, quantified as population density, and of the spatial arrangement of the habitat in the landscape and not of the geographical distance per se. In fragmented habitats, random genetic drift was the prevailing force for sampling sites separated by a few hundred meters.
Background: Growth rate is central to the development of cells in all organisms. However, little is known about the impact of changing growth rates. We used continuous cultures to control growth rate and studied the transcriptional program of the model eukaryote Saccharomyces cerevisiae, with generation times varying between 2 and 35 hours.
Results: A total of 5930 transcripts were identified at the different growth rates studied. Consensus clustering of these revealed that half of all yeast genes are affected by the specific growth rate, and that the changes are similar to those found when cells are exposed to different types of stress (>80% overlap). Genes with decreased transcript levels in response to faster growth are largely of unknown function (>50%) whereas genes with increased transcript levels are involved in macromolecular biosynthesis such as those that encode ribosomal proteins. This group also covers most targets of the transcriptional activator RAP1, which is also known to be involved in replication. A positive correlation between the location of replication origins and the location of growth-regulated genes suggests a role for replication in growth rate regulation.
Conclusion: Our data show that the cellular growth rate has great influence on transcriptional regulation. This, in turn, implies that one should be cautious when comparing mutants with different growth rates. Our findings also indicate that much of the regulation is coordinated via the chromosomal location of the affected genes, which may be valuable information for the control of heterologous gene expression in metabolic engineering.
The main purpose of the paper is to explain the divergent paths of development of ethno-territorial protest movements in modern democratic political systems. By focusing on the interaction between these movements and the state, the different systems of accommodation between the relevant regional and central elites will be analyzed. The study concentrates on the case studies of Québec (Canada) and Corsica (France). The paper is divided into three parts. The first part describes the traditional systems of accommodation in France and Canada. The second part is focused on the process of socio-economic modernization in the 1950s and 1960s in those countries that threatened the established patterns of elite accommodation. The third part deals with the consequences for the established patterns of elite-accommodation and new concepts of territorial management that the central states tried to establish. By looking at the different degrees of centralization and decentralization in the mentioned political systems, the question of access to the political system by new social and political actors will be discussed in detail.
Length variations of repetitive sequences in different AT-rich loop-coding regions of mitochondrial 16S rDNA in two gastropod species were discovered during intraspecific haplotype surveys. Examination of the discrete length variation of the basic repeat unit in a phylogenetic framework led to the conclusion of a microsatellite-like mutational dynamic. The observations suggest that the presence of a repetitive sequence structure alone is sufficient to trigger this dynamic.
I analysed the importance of shell size, shell shape, habitat preferences and availability, experienced climate, active dispersal and influence of Pleistocene glaciations for the range sizes of 37 Western Palaearctic Helicidae s.l. species for which a phylogeny was available. In both cross-species and phylogenetically controlled analyses, the range sizes were positively correlated to climatic tolerance, shell size, active dispersal and influence of Pleistocene glaciations. In addition, range sizes increased significantly with latitude. Multiple regression suggested that, predominantly, the influence of Pleistocene glaciations, tolerance to large annual temperature ranges and shell size influenced the distributional range sizes. Habitat preference, range and availability, active dispersal and shell shape explained no additional variance. The results suggest that the processes influencing species range size of the Helicidae s.l. are mainly related to the climatic shifts after the Pleistocene.
Alzheimer's disease-related mutations in the presenilin-1 gene (PS1) are leading to an elevated production of neurotoxic beta-amyloid 1-42 and may additionally enhance oxidative stress. Here, we provide in vivo evidence indicating that brains of transgenic mice expressing different human Alzheimer-linked PS1 mutations exhibit a reduced activity of two antioxidant enzymes. For this purpose, mice transgenic for human PS1 and for single and multiple PS1 mutations were generated. Mice with multiple PS1 mutations showed a significantly decreased activity of the antioxidant enzymes Cu/Zn superoxide dismutase and glutathione reductase already at an age of 3-4 months. As expected, this effect was less pronounced for the mice with a single PS1 mutation. By contrast, animals bearing normal human PS1 showed significantly elevated enzyme activities relative to non-transgenic littermate controls.
Background Reliable taxonomic identification at the species level is the basis for many biological disciplines. In order to distinguish species, it is necessary that taxonomic characters allow for the separation of individuals into recognisable, homogeneous groups that differ from other such groups in a consistent way. We compared here the suitability and efficacy of traditionally used shell morphology and DNA-based methods to distinguish among species of the freshwater snail genus Radix (Basommatophora, Pulmonata). Results Morphometric analysis showed that shell shape was unsuitable to define homogeneous, recognisable entities, because the variation was continuous. On the other hand, the Molecularly defined Operational Taxonomic Units (MOTU), inferred from mitochondrial COI sequence variation, proved to be congruent with biological species, inferred from geographic distribution patterns, congruence with nuclear markers and crossing experiments. Moreover, it could be shown that the phenotypically plastic shell variation is mostly determined by the environmental conditions experienced. Conclusion Contrary to DNA-taxonomy, shell morphology was not suitable for delimiting and recognising species in Radix. As the situation encountered here seems to be widespread in invertebrates, we propose DNA-taxonomy as a reliable, comparable, and objective means for species identification in biological research.
Enhanced apoptosis and elevated levels of reactive oxygen species (ROS) play a major role in aging. In addition, several neurodegenerative diseases are associated with increased oxidative stress and apoptosis in neuronal tissue. Antioxidative treatment has neuro-protective effects. The aim of the present study was to evaluate changes of susceptibility to apoptotic cell death by oxidative stress in aging and its inhibition by the antioxidant Ginkgo biloba extract EGb761. We investigated basal and ROS-induced levels of apoptotic lymphocytes derived from the spleen in young (3 months) and old (24 months) mice. ROS were induced by 2-deoxy-D-ribose (dRib) that depletes the intracellular pool of reduced glutathione. Lymphocytes from aged mice accumulate apoptotic cells to a significantly higher extent under basal conditions compared to cells from young mice. Treatment with dRib enhanced this difference, implicating a higher sensitivity to ROS in aging. Apoptosis can be reduced in vitro by treatment with EGb761. In addition, mice were treated daily with 100mg/kg EGb761 per os over a period of two weeks. ROS-induced apoptosis was significantly reduced in the EGb761 group. Interestingly, this effect seemed to be more pronounced in old mice.
Apoptosis seems to be involved in immunosenescence associated with aging. Moreover, in lymphocytes (PBL) of patients with Alzheimer's disease, an increased susceptibility to the apoptotic pathway has been described possibly due to impaired protection of oxidative stress. Accordingly, it seemed to be of particular interest to investigate the contribution of normal aging to the susceptibility from human lymphocytes to programmed cell death. We could show that PBL from elderly individuals (>60 years) accumulate apoptosing cells to a significant higher extent in spontaneous and activation-induced cell death compared to younger controls (<35 years). Treatment with the oxidative stressor 2-deoxy-D-ribose or with agonistic-CD95-antibody pronounced this effect even more implicating a higher sensitivity to reactive oxygen species and a higher functional CD95 expression, respectively. In addition, expression of the activation markers HLA-DR and CD95 was significantly increased in CD3+-cells of aged subjects, while expression of CD25 did not seem to be affected by age. Expression of Bcl-2 was increased in aging and correlated with the number of apoptotic cells.
The relevance of physiological immune aging is of great interest with respect to determining disorders with pathologic immune function in aging individuals. In recent years, the relevance of changes in peripheral lymphocytes in age-associated neurologic diseases has become more evident. Due to the lack of immunological studies, covering more than one event after mitogenic activation, we envisaged a new concept in the present study, aiming to investigate several events, starting from T cell receptor (TCR) ligation up to T cell proliferation. In addition, we addressed the question whether changes are present in the subsets (CD4, CD8) with aging. Phosphorylation of tyrosine residues declines with increasing age in CD4+ cells. Fewer levels of CD69 positive cells after 4 h mitogenic activation, altered expression of cytokines (IL2, IFN-gamma and TNF-alpha; 22 h) and lower proliferation (72 h) were determined in aging. Moreover, it could be shown that CD8+ lymphocytes react more effectively to mitogenic stimulation with reference to CD69 expression and proliferation in both age groups (<35 and >60 years old). These data indicate that T cell activation, mediated by TCR engagement, is significantly impaired in aging and both subsets are affected. However, bypassing the TCR does not fully restore T cell function, indicating that there are more mechanisms involved than impaired signal transduction through TCR only. The results will be discussed in relation to their relevance in neurodegenerative and psychiatric disorders.
In vivo manipulation of interleukin-2 expression by a retroviral tetracycline (tet)-regulated system
(1999)
We have used the tetracycline (tet)-regulated system as described previously to evaluate the applicability of controlled gene expression in cancer gene therapy. As a model gene, we used the human interleukin-2 (IL-2) gene, which has been placed under the transcriptional control of the tetO/promoter. Human melanoma cells were transduced by two modified retroviral tet vectors containing the transactivator regulatory unit and the IL-2 gene driven by the tetO/promoter, respectively. In the absence of tet, IL-2 expression in the target cells was stable over several months. IL-2 production was in the range of 40 U/106 cells/24 hours. A fine tuning of IL-2 expression could be achieved by culturing the transduced cells with increasing doses of tet, whereby a concentration of 500 ng/mL tet in the culture medium abrogated IL-2 expression. Most importantly for clinical application, IL-2 expression by the transduced melanoma cells could also be regulated in vivo. When nu/nu mice were inoculated with the transduced tumor cells, they failed to develop tumors. Instead, the inhibition of IL-2 expression in the transduced tumor cells by oral administration of tet led to subcutaneous tumor growth; this growth rate was comparable with the growth rate of subcutaneously inoculated untransduced parental cells. The finding demonstrates the applicability of the tet-regulated system in cancer gene therapy.
Many cases of early-onset inherited Alzheimer's disease (AD) are caused by mutations in the presenilin-1 (PS1) gene. Expression of PS1 mutations in cell culture systems and in primary neurons from transgenic mice increases their vulnerability to cell death. Interestingly, enhanced vulnerability to cell death has also been demonstrated for peripheral lymphocytes from AD patients. We now report that lymphocytes from PS1 mutant transgenic mice show a similar hypersensitivity to cell death as do peripheral cells from AD patients and several cell culture systems expressing PS1 mutations. The cell death-enhancing action of mutant PS1 was associated with increased production of reactive oxygen species and altered calcium regulation, but not with changes of mitochondrial cytochrome c. Our study further emphasizes the pathogenic role of mutant PS1 and may provide the fundamental basis for new efforts to close the gap between studies using neuronal cell lines transfected with mutant PS1, neurons from transgenic animals, and peripheral cells from AD patients. Copyright 2001 Academic Press.
Neben dem alltagsbegrifflichen Verständnis der "Unternehmer", das deren Lagerbildung und Interessenhomogenität unterstellt, lassen sich bei genauerem Hinsehen immer schon interne Differenzierungen erkennen, erfassbar v.a. mit der Kategorie der "Kapitalfraktionen". Öffentliche politische Interventionen des "Unternehmerlagers" erscheinen wiederum auf den ersten Blick homogen. Rolf Schmucker zeigt empirisch, dass auch dieser Blick trügt.
Klassenmedizin
(2003)
In large models of neuronal cell death, there is a tight correlation between Cdk5 deregulation and cell-cycle dysfunction. However, pathways that link Cdk5 to the cell cycle during neuronal death are still unclear. We have investigated the molecular events that precede p25/Cdk5-triggered neuronal death using a neuronal cell line that allows inducible p25 expression. In this system, no sign of apoptosis was seen before 24 hours of p25 induction. Thus, at that time, cell-cycle-regulatory proteins were analysed by immunoblotting and some of them showed a significant deregulation. Interestingly, after time-course experiments, the earliest feature correlated with p25 expression was the phosphorylation of the retinoblastoma protein (Rb). Indeed, this phosphorylation was observed 6 hours after p25 induction and was abolished in the presence of a Cdk5 inhibitor, roscovitine, which does not inhibit the usual Rb cyclin-D kinases Cdk4 and Cdk6. Furthermore, analyses of levels and subcellular localization of Cdk-related cyclins did not reveal any change following Cdk5 activation, arguing for a direct effect of Cdk5 activity on Rb protein. This latter result was clearly demonstrated by in vitro kinase assays showing that the p25-Cdk5 complex in our cell system phosphorylates Rb directly without the need for any intermediary kinase activity. Hence, Rb might be an appropriate candidate that connects Cdk5 to cell-cycle deregulation during neuronal cell death.
The identification of specific genetic (presenilin-1 [PS1] and amyloid precursor protein [APP] mutations) and environmental factors responsible for Alzheimer's disease (AD) has revealed evidence for a shared pathway of neuronal death. Moreover, AD-specific cell defects may be observed in many other nonneuronal cells (e.g., lymphocytes). Thus, lymphocytes may serve as a cellular system in which to study risk factors of sporadic, as well as genetic AD in vivo. The aim of our present study was to clarify whether lymphocytes bearing genetic or sporadic risk factors of AD share an increased susceptibility to cell death. Additionally we examined whether a cell typespecific vulnerability pattern was present and how normal aging, the main risk factor of sporadic AD, contributes to changes in susceptibility to cell death. Here, we report that lymphocytes affected by sporadic or genetic APP and PS1 AD risk factors share an increased vulnerability to cell death and exhibit a similar cell type-specific pattern, given that enhanced vulnerability was most strongly developed in the CD4+ T-cell subtype. In this paradigm, sporadic risk factors revealed the highest impact on cell type-specific sensitivity of CD4+ T cells to apoptosis. In contrast, normal aging results in an increased susceptibility to apoptosis of both, CD4+ and CD8+ T cells.
Introduction: Reactive oxygen species (ROS) have been implicated in neurodegeneration and seem to be involved in the physiology and pathophysiology of several diseases, including normal aging and Alzheimer’s disease (AD). Enhanced ROS production in aging or AD is not restricted to the brain, but can also been seen in several peripheral tissues. The objective of the present study was to evaluate whether the mechanisms involved in the generation of oxidative stress in normal senescence and Alzheimer’s disease are identical or not. Methods: We analysed intracellular basal levels of ROS in lymphocytes from AD patients and healthy young and aged not-demented subjects as well as ROS levels following stimulation with d-ribose and staurosporine in all three groups. ROS levels were measured by flow cytometry using the intracellular fluorescence dye dihydrorhodamine123 (DHR123). Results: Our study shows that AD lymphocytes have increased basal levels of ROS, low susceptibility to ROS stimulation by 2-deoxy-D-ribose (dRib) and an increased response to staurosporine when compared with age-matched controls. Discussion: The data suggest that the defect(s) responsible for enhanced ROS production in AD may involve different or additional biological pathways than those involved in enhanced ROS generation during aging.
As major sources of reactive oxygen species (ROS), mitochondrial structures are exposed to high concentrations of ROS and may therefore be particularly susceptible to oxidative damage. Mitochondrial damage could play a pivotal role in the cell death decision. A decrease in mitochondrial energy charge and redox state, loss of transmembrane potential (depolarization), mitochondrial respiratory chain impairment, and release of substances such as calcium and cytochrome c all contribute to apoptosis. These mitochondrial abnormalities may constitute a part of the spectrum of chronic oxidative stress in Alzheimer's disease. Accumulation of amyloid beta (Abeta) in form of senile plaques is also thought to play a central role in the pathogenesis of Alzheimer's disease mediated by oxidative stress. In addition, increasing evidence shows that Abeta generates free radicals in vitro, which mediate the toxicity of this peptide. In our study, PC12 cells were used to examine the protective features of EGb 761(definition see editorial) on mitochondria stressed with hydrogen peroxide and antimycin, an inhibitor of complex III. In addition, we investigated the efficacy of EGb 761 in Abeta-induced MTT reduction in PC12 cells. Moreover, we examined the effects of EGb 761 on ROS levels and ROS-induced apoptosis in lymphocytes from aged mice after in vivo administration. Here, we will report that EGb 761 was able to protect mitochondria from the attack of hydrogen peroxide, antimycin and Abeta. Furthermore, EGb 761 reduced ROS levels and ROS-induced apoptosis in lymphocytes from aged mice treated orally with EGb 761 for 2 weeks. Our data further emphasize neuroprotective properties of EGb 761, such as protection against Abeta-toxicity, and antiapoptotic properties, which are probably due to its preventive effects on mitochondria.
FGF-2, a potent multifunctional and neurotrophic growth factor, is widely expressed in the brain and upregulated in cerebral ischemia. Previous studies have shown that intraventricularly or systemically administered FGF-2 reduces the size of cerebral infarcts. Whether endogenous FGF-2 is beneficial for the outcome of cerebral ischemia has not been investigated. We have used mice with a null mutation of the fgf2 gene to explore the relevance of endogenous FGF-2 in brain ischemia. Focal cerebral ischemia was produced by occlusion of the middle cerebral artery (MCAO). We found a 75% increase in infarct volume in fgf2 knock-out mice versus wild type littermates (P < 0.05). This difference in the extent of ischemic damage was observed after 24 h, and correlated with decreased viability in fgf2 mutant mice following MCA occlusion. Increased infarct volume in fgf2 null mice was associated with a loss of induction in hippocampal BDNF and trkB mRNA expression. These findings indicate that signaling through trkB may contribute to ameliorating brain damage following ischemia and that bdnf and trkB may be target genes of FGF-2. Together, our data provide the first evidence that endogenous FGF-2 is important in coping with ischemic brain damage suggesting fgf2 as one crucial target gene for new therapeutic strategies in brain ischemia.
Im März 2000 hat der Europäische Rat von Lissabon das strategische Ziel der EU formuliert, "die Union zum wettbewerbsfähigsten und dynamischsten wissensbasierten Wirtschaftsraum der Welt zu machen – ... der fähig ist, ein dauerhaftes Wirtschaftswachstum mit mehr und besseren Arbeitsplätzen und einem größeren sozialen Zusammenhalt zu schaffen". Die Umsetzung soll u. a. durch die "Offene Methode der Koordinierung" (OMK) vorangetrieben werden. Damit wird eine Leitungs- und Koordinierungsfunktion des Rates auch auf solchen Politikfeldern angestrebt, die nach dem Vertragsrecht in die alleinige Gestaltungskompetenz der Mitgliedstaaten fallen. Die OMK lässt sich als eine Art Benchmarking-Verfahren ohne unmittelbar verpflichtende Konsequenzen für die Mitgliedstaaten beschreiben. Ein ähnliches Vorgehen wird seit 1997 in der Beschäftigungspolitik praktiziert. Mit der Lissabon-Strategie wird die OMK sukzessive auf weitere sozialpolitische Bereiche ausgedehnt. Im Bereich der Alterssicherung kommt sie ebenso zum Einsatz wie bei der Bekämpfung von Armut und sozialer Ausgrenzung. Künftig wird auch die Gesundheitspolitik der Mitgliedsländer auf dem Prüfstand der OMK stehen.
Die Rolle des Kulturbildes im interkulturell motivierten Deutschunterricht am Beispiel des Iran
(2006)
Der Vortrag basiert auf meiner Dissertation über die "Interkulturelle Kommunikation an iranischen Sprachinstituten und Universitäten". Er setzt sich zunächst mit den Vorstellungen über die Kultur des deutschsprachigen Raums im Iran und dessen Reflexion in Unterrichtsprozessen auseinander. In diesem Zusammenhang wird die Rolle der Beziehung zwischen Kultur und Sprache im Fremdsprachenunterricht herausgearbeitet. Daher steht die Beschäftigung mit Kulturfragen im Mittelpunkt. Es handelt sich dabei vor allem um zwei typische Beispiele für "interkulturelle Missverständnisse", die bei iranischen Deutschlernenden in ihrem Verständnis der deutschen Kultur sehr häufig vorkommen. Zwei weitere Beispiele werden gezeigt, in denen die kontrastierten Kulturen miteinander übereinstimmen und somit eine feste und unproblematische Grundlage für den Umgang miteinander darstellen. Im Folgenden soll die Rolle der Deutschlehrer und der institutionellen Bedingungen im Unterricht analysiert werden. Gerade der Deutschunterricht in islamischen Ländern ist darauf angewiesen, dass der Lehrer Sprachsituationen bereitstellt, anhand derer er versucht, die von der Ausgangsgesellschaft stammenden interkulturellen Konflikte aufzugreifen und letztlich zu bewältigen. Meine Unterrichtsanalysen zeigen, dass diese Vorgehensweise der Lehrer ohne Steuerung der Institution nicht möglich ist. Da die interkulturell motivierte Deutschvermittlung institutionelle Voraussetzungen verlangt, stellt der Vortrag auch die Frage, ob eine regionale von den beiden Regierungen unabhängige Institution für die Beschäftigung mit den möglichen interkulturellen Konflikten notwendig ist und warum sie bis jetzt nicht zustande gekommen ist. Trotz der neusten politischen Bemühungen um die kulturellen Annäherungen zwischen beiden Ländern gelten immer noch beide Kulturen als "kontrastiert". Der Deutschunterricht in der iranischen Gesellschaft kann diese Kluft nur dann schmälern, wenn die Deutsch anbietenden Institutionen inter- und transkulturelle Aspekte stärker berücksichtigen.
Since the early 1960s ethno-territorial movements have increasingly challenged established patterns of political integration in western democracies. The purpose of this paper is to compare the formation and different paths of development of such nationalist movements in Quebec (Canada) and Corsica (France). The paper presents a brief outline of an argument more fully developed in a study on the two cases. After a short introduction I will first discuss theoretical aspects of minority nationalism. Secondly, I will try to give an overview over major findings and conclusions of my study.
Background The reciprocal (9;22) translocation fuses the bcr (breakpoint cluster region) gene on chromosome 22 to the abl (Abelson-leukemia-virus) gene on chromosome 9. Depending on the breakpoint on chromosome 22 (the Philadelphia chromosome – Ph+) the derivative 9+ encodes either the p40(ABL/BCR) fusion transcript, detectable in about 65% patients suffering from chronic myeloid leukemia, or the p96(ABL/BCR) fusion transcript, detectable in 100% of Ph+ acute lymphatic leukemia patients. The ABL/BCRs are N-terminally truncated BCR mutants. The fact that BCR contains Rho-GEF and Rac-GAP functions strongly suggest an important role in cytoskeleton modeling by regulating the activity of Rho-like GTPases, such as Rho, Rac and cdc42. We, therefore, compared the function of the ABL/BCR proteins with that of wild-type BCR. Methods We investigated the effects of BCR and ABL/BCRs i.) on the activation status of Rho, Rac and cdc42 in GTPase-activation assays; ii.) on the actin cytoskeleton by direct immunofluorescence; and iii) on cell motility by studying migration into a three-dimensional stroma spheroid model, adhesion on an endothelial cell layer under shear stress in a flow chamber model, and chemotaxis and endothelial transmigration in a transwell model with an SDF-1α gradient. Results Here we show that both ABL/BCRs lost fundamental functional features of BCR regarding the regulation of small Rho-like GTPases with negative consequences on cell motility, in particular on the capacity to adhere to endothelial cells. Conclusion Our data presented here describe for the first time an analysis of the biological function of the reciprocal t(9;22) ABL/BCR fusion proteins in comparison to their physiological counterpart BCR.
Kurzfassung eines Vortrags gehalten auf der GMA-Tagung vom 04.11.-06.11.2005 in Münster. Aufgrund wenig überzeugender Leistungen seiner Studierenden im vorklinischen Studienabschnitt hat der Fachbereich Medizin der Goethe-Universität zum Wintersemester 2001/02 eine völlig neue Studienordnung eingeführt, deren wesentliche Ziele das erfolgreiche Erlernen natur-, geistes- und sozialwissenschaftlicher Komponenten der normalen Biologie des Menschen mit optimaler Vorbereitung auf den klinischen Studienabschnitt binnen zweier Studienjahre und die Kompatibilität mit der ÄAppO und der ZÄAppO (jeweils alte und neue Version bzw. Vorentwurf) sind. Grundprinzipien des Curriculums sind: 1. Logisch aufeinander abgestimmte Themenblöcke, 2. Integration von makroskopischer und mikroskopischer Anatomie, 3. Interdisziplinärität der Themenblöcke, 4. Immanente Wiederholung von Lehrinhalten durch enge Verzahnung von Plenar- und Kleingruppenveranstaltungen, 5. Aufteilung der Studienanfänger in gemischte Gruppen nach den Kriterien Geschlecht, Herkunft und Studienfach, 6. Erlernen des Umgangs mit Patienten schon vom ersten Semester an durch "Anatomie am Lebenden"-Kurse und durch den Kurs Einführung in die klinische Medizin. Wichtigste Ergebnisse: Steigerung der Referenzgruppe beim Physikum von ca. 25% auf >60%, relativ guter Prüfungserfolg, hervorragende Sozialisation durch die frühe randomisierte Zuordnung in Kleingruppen und der sehr glatte Übergang in den klinischen Abschnitt des Medizin- bzw. Zahnmedizinstudiums.
Von Ahasver, dem ‚Ewigen Juden’ wird schon im Druck von 1602 erzählt, er habe, nachdem er von Christus zur ewigen Wanderschaft verdammt worden war, die Stadt Jerusalem verlassen und durch alle Teile der Welt wandern müssen. Als er nach Jahrhunderten wieder in das Heilige Land gekommen sei, habe er das Land verwüstet und Jerusalem so vollständig zerstört vorgefunden, dass er es nicht mehr erkannt habe. Mit dieser Bemerkung signalisiert der Autor des kleinen, aber überaus wirkungsmächtigen Traktats den Lesern, dass damit mehr gemeint ist als nur eine Episode auf der Wanderschaft Ahasvers. [...]
Gute naturwissenschaftliche Vorkenntnisse sind insbesondere für den vorklinischen Studienabschnitt wichtig. Wegen der heterogenen Auswahl von Leistungskursen und Abituranforderungen kann jedoch nicht unbedingt von einem einheitlichen Wissensstand ausgegangen werden. Daher wurde versucht, mit einem Testbogen aus insgesamt 40 Aufgaben zur Biologie, Chemie, Mathematik und Physik den Wissensstand der Studienanfänger in Humanmedizin in Deutschland zu quantifizieren. Der Fragebogen enthielt neben Faktenaufgaben auch Anwendungen vor allem mathematischer und chemischer Prinzipien. Alle Fragen mussten durch Freitextantworten oder Skizzen beantwortet werden. Teilgenommen haben insgesamt 2 935 Studienanfänger des Wintersemesters 2004/2005 von 14 deutschen Universitäten (etwa 40% des Jahrganges). Im Mittel wurden 14,34 der 40 Aufgaben richtig beantwortet; etwas bessere Kenntnisse wurden in den 15 Biologiefragen (6,89) und den 8 Mathematikfragen erreicht (3,23), während vor allem in Chemie (2,18 von 10 Fragen) und Physik (1,55 von 8 Fragen) große Wissenslücken bestehen. Die Ergebnisse bestätigen, dass die naturwissenschaftlichen Vorkenntnisse der Studienanfänger schlecht sind; sie erfordern einen größeren Zeitaufwand für die Vermittlung des Abiturwissens im ersten vorklinischen Semester. Sinnvoll erscheint alternativ die verpflichtende Teilnahme an Zusatzkursen in diesen Fächern vor Aufnahme des eigentlichen Fachstudiums.
Mit der Neuregelung der Studienplatzverteilung von 2005 haben die Universitäten in Deutschland die Möglichkeit, bis zu 60% der Studienplätze nach universitätseigenen Kriterien zu vergeben. Implizit wird vom Gesetzgeber und der öffentlichen Meinung gefordert, nicht-leistungsbezogene Kriterien und Persönlichkeitsmerkmale verstärkt zur Bewerberauswahl einzusetzen (Motivation, Identifikation, Vermeidung von Fehlvorstellungen). Da in Anbetracht der Bewerberzahlen mündliche Auswahlgespräche als ungeeignet erscheinen, wurde vom Fachbereich Medizin der Johann Wolfgang Goethe Universität ein Fragebogen entworfen, um nichtschulische Leistungen zu erfassen. Dieser Fragebogen wurde am Beginn des Wintersemesters 2005/2006 von allen Studienanfängern der JWG-Universität Frankfurt und der Medizinischen Universität Innsbruck ausgefüllt. Entgegen der initialen Erwartungen der Verfasser gaben nur etwa 15% Prozent Medizin-spezifische berufliche Vorerfahrungen an (Rettungsdienst, Ausbildung als Krankenschwester/pfleger oder ähnliches); dagegen wurden von etwa 60% angegeben, mindestens ein Musikinstrument zu spielen oder länger sportlich aktiv gewesen zu sein. Die Zusammenstellung der Selbstangaben zeigt, dass Medizin-relevante Vorkenntnisse nur bei einem kleinen Anteil der Studienbewerber in größerem Umfang vorhanden sind. Aufgrund der großen Streuung in der Art und Dauer der angegebenen Vorleistungen sollte die Erhebung von Parametern zur Beurteilung von soft skills, z.B. durch Online-Fragebogen, als (Vor)Selektionsinstrument nur sehr vorsichtig eingesetzt werden.
Maßgeblich unter dem wachsenden externen Druck hat die didaktische Qualifizierung in den medizinischen Fakultäten an Bedeutung gewonnen. Im Rahmen der Professionalisierung der medizinischen Aus-, Fort- und Weiterbildung ist eine pädagogisch-didaktische Ausbildung der Lehrenden unumgänglich. Um Orientierung und Argumentationshilfe zu geben, werden in einer dreiteiligen Artikelfolge Stellenwert der Medizindidaktik, Anforderungsprofil der Angebote und Konzepte zur Implementierung und zur Erfolgsmessung für den deutschsprachigen Raum beleuchtet. In Teil II beleuchten wir den Ausbildungsbedarf und erstellen ein Anforderungsprofil für ein strukturiertes systematisches Qualifizierungsangebot. Der Hauptbedarf besteht in der Qualifizierung der Lehrenden, die den täglichen Unterricht durchführen. Insbesondere sie brauchen das Handwerkszeug, das ihnen erlaubt, ihre verschiedenen Lehraufgaben effizienter zu bewältigen. Seitens der Fakultät und der Studierenden bestehen Ansprüche an qualifizierte Lehrende; ebenso bestehen Forderungen seitens der Lehrenden an adäquat qualifizierende Kurse. Zur Umsetzung dieser berechtigten Ansprüche sind modular aufgebaute Programme nötig, die eine zumindest national qualitativ und quantitativ vergleichbare Ausbildung gewährleisten. Anforderungen an derartige Kurse sind bereits definiert und lokal zum Beispiel in Baden-Württemberg und zum Teil auch in Nordrhein-Westfalen umgesetzt. Es gilt nun, diese auf breiter Basis in die Praxis umzusetzen.
Alle Leistungsnachweise des klinischen Studienabschnittes nach neuer Ärztlicher Approbationsordnung müssen benotet werden; hierzu sind in der Regel schriftliche Prüfungen notwendig. Bisher erprobte Methoden beinhalten die Prüfung passiven Wissens (Einfachauswahlfragen, multiple choice-Fragen, progress test-Fragen) und aktiven Wissens (short essay questions, long essay questions). Vor- und Nachteile dieser Verfahren werden diskutiert, sowie die zur Erstellung, Durchführung und Auswertung schriftlicher Prüfungen notwendigen Ressourcen.
Maßgeblich unter dem wachsenden externen Druck hat die didaktische Qualifizierung in den medizinischen Fakultäten an Bedeutung gewonnen. Im Rahmen der Professionalisierung der medizinischen Aus-, Fort- und Weiterbildung ist eine pädagogisch-didaktische Ausbildung der Lehrenden unumgänglich. Um Orientierung und Argumentationshilfe zu geben, werden in einer dreiteiligen Artikelfolge Stellenwert der Medizindidaktik, Anforderungsprofil der Angebote und Konzepte zur Implementierung und zur Erfolgsmessung für den deutschsprachigen Raum beleuchtet. In Teil I geben wir eine Bestandsaufnahme zur Medizindidaktik. Aktuell gibt es bundesweit ein breit gefächertes Qualifizierungsangebot. Es reicht von einfachen unstrukturierten Kurzfortbildungen wie zum Beispiel Vorträgen und Seminaren, die inhaltlich, formal und qualitativ eine große Beliebigkeit zeigen, bis hin zu umfassenden mehrjährigen (Aufbau-)Studiengängen mit "Master-Degree". Im internationalen Vergleich fehlt in Deutschland ein allgemein verbindliches "Basis-Programm", das die täglich Lehrenden systematisch auf ihre Ausbildungsaufgaben vorbereitet. Dies ist bisher nur lokal umgesetzt wie zum Beispiel in Baden-Württemberg mit dem ministeriell zertifizierten Programm der Medizindidaktischen Qualifikation I und II. Vergleichbares ist in Nordrhein-Westfalen und Bayern im Aufbau.
The interaction of T cells and antigen-presenting cells is central to adaptive immunity and involves the formation of immunological synapses in many cases. The surface molecules of the cells form a characteristic spatial pattern whose formation mechanisms and function are largely unknown. We perform computer simulations of recent experiments on geometrically repatterned immunological synapses and explain the emerging structure as well as the formation dynamics. Only the combination of in vitro experiments and computer simulations has the potential to pinpoint the kind of interactions involved. The presented simulations make clear predictions for the structure of the immunological synapse and elucidate the role of a self-organizing attraction between complexes of T cell receptor and peptide–MHC molecule, versus a centrally directed motion of these complexes.
Enzymatic and antisense effects of a specific anti-Ki-ras ribozyme in vitro and in cell culture
(1999)
Due to their mode of action, ribozymes show antisense effects in addition to their specific cleavage activity. In the present study we investigated whether a hammerhead ribozyme is capable of cleaving mutated Ki-ras mRNA in a pancreatic carcinoma cell line and whether antisense effects contribute to the activity of the ribozyme. A 2[prime]-O-allyl modified hammerhead ribozyme was designed to cleave specifically the mutated form of the Ki-ras mRNA (GUU motif in codon 12). The activity was monitored by RT-PCR on Ki-ras RNA expression by determination of the relative amount of wild type to mutant Ki-ras mRNA, by 5-bromo-2[prime]-deoxy-uridine incorporation on cell proliferation and by colony formation in soft agar on malignancy in the human pancreatic adenocarcinoma cell line CFPAC-1, which is heterozygous for the Ki-ras mutation. A catalytically inactive ribozyme was used as control to differentiate between antisense and cleavage activity and a ribozyme with random guide sequences as negative control. The catalytically active anti-Ki-ras ribozyme was at least 2-fold more potent in decreasing cellular Ki-ras mRNA levels, inhibiting cell proliferation and colony formation in soft agar than the catalytically inactive ribozyme. The catalytically active anti-Ki-ras ribozyme, but not the catalytically inactive or random ribozyme, increased the ratio of wild type to mutated Ki-ras mRNA in CFPAC-1 cells. In conclusion, both cleavage activity and antisense effects contribute to the activity of the catalytically active anti-Ki-ras hammerhead ribozyme. Specific ribozymes might be useful in the treatment of pancreatic carcinomas containing an oncogenic GTT mutation in codon 12 of the Ki-ras gene.
Cäcilia
(1803)
The conditionally-lethal pso4-1 mutant allele of the spliceosomal-associated PRP19 gene allowed us to study this gene’s influence on pre-mRNA processing, DNA repair and sporulation. Phenotypes related to intron-containing genes were correlated to temperature. Splicing reporter systems and RT–PCR showed splicing efficiency in pso4-1 to be inversely correlated to growth temperature. A single amino acid substitution, replacing leucine with serine, was identified within the N-terminal region of the pso4-1 allele and was shown to affect the interacting properties of Pso4-1p. Amongst 24 interacting clones isolated in a two-hybrid screening, seven could be identified as parts of the RAD2, RLF2 and DBR1 genes. RAD2 encodes an endonuclease indispensable for nucleotide excision repair (NER), RLF2 encodes the major subunit of the chromatin assembly factor I, whose deletion results in sensitivity to UVC radiation, while DBR1 encodes the lariat RNA splicing debranching enzyme, which degrades intron lariat structures during splicing. Characterization of mutagen-sensitive phenotypes of rad2{Delta}, rlf2{Delta} and pso4-1 single and double mutant strains showed enhanced sensitivity for the rad2{Delta} pso4-1 and rlf2{Delta} pso4-1 double mutants, suggesting a functional interference of these proteins in DNA repair processes in Saccharomyces cerevisiae.
Chemically modified bases are frequently used to stabilize nucleic acids, to study the driving forces for nucleic acid structure formation and to tune DNA and RNA hybridization conditions. In particular, fluorobenzene and fluorobenzimidazole base analogues can act as universal bases able to pair with any natural base and to stabilize RNA duplex formation. Although these base analogues are compatible with an A-form RNA geometry, little is known about the influence on the fine structure and conformational dynamics of RNA. In the present study, nano-second molecular dynamics (MD) simulations have been performed to characterize the dynamics of RNA duplexes containing a central 1'-deoxy-1'-(2,4-difluorophenyl)-ß-D-ribofuranose base pair or opposite to an adenine base. For comparison, RNA with a central uridine:adenine pair and a 1'-deoxy-1'-(phenyl)-ß-D-ribofuranose opposite to an adenine was also investigated. The MD simulations indicate a stable overall A-form geometry for the RNAs with base analogues. However, the presence of the base analogues caused a locally enhanced mobility of the central bases inducing mainly base pair shear and opening motions. No stable ‘base-paired’ geometry was found for the base analogue pair or the base analogue:adenine pairs, which explains in part the universal base character of these analogues. Instead, the conformational fluctuations of the base analogues lead to an enhanced accessibility of the bases in the major and minor grooves of the helix compared with a regular base pair.
The radiation-sensitive mutant pso4-1 of Saccharomyces cerevisiae shows a pleiotropic phenotype, including sensitivity to DNA cross-linking agents, nearly blocked sporulation and reduced mutability. We have cloned the putative yeast DNA repair gene PSO4 from a genomic library by complementation of the blocked UV-induced mutagenesis and of sporulation in diploids homozygous for pso4-1. Sequence analysis revealed that gene PSO4 consists of 1512 bp located upstream of UBI4 on chromosome XII and encodes a putative protein of 56.7 kDa. PSO4 is allelic to PRP19, a gene encoding a spliceosome-associated protein, but shares no significant homology with other yeast genes. Gene disruption with a destroyed reading frame of our PSO4 clone resulted in death of haploid cells, confirming the finding that PSO4/PRP19 is an essential gene. Thus, PSO4 is the third essential DNA repair gene found in the yeast S.cerevisiae.
Mismatch repair is a highly conserved system that ensures replication fidelity by repairing mispairs after DNA synthesis. In humans, the two protein heterodimers hMutSα (hMSH2‐hMSH6) and hMutLα (hMLH1‐hPMS2) constitute the centre of the repair reaction. After recognising a DNA replication error, hMutSα recruits hMutLα, which then is thought to transduce the repair signal to the excision machinery. We have expressed an ATPase mutant of hMutLα as well as its individual subunits hMLH1 and hPMS2 and fragments of hMLH1, followed by examination of their interaction properties with hMutSα using a novel interaction assay. We show that, although the interaction requires ATP, hMutLα does not need to hydrolyse this nucleotide to join hMutSα on DNA, suggesting that ATP hydrolysis by hMutLα happens downstream of complex formation. The analysis of the individual subunits of hMutLα demonstrated that the hMutSα–hMutLα interaction is predominantly conferred by hMLH1. Further experiments revealed that only the N‐terminus of hMLH1 confers this interaction. In contrast, only the C‐terminus stabilised and co‐immunoprecipitated hPMS2 when both proteins were co‐expressed in 293T cells, indicating that dimerisation and stabilisation are mediated by the C‐terminal part of hMLH1. We also examined another human homologue of bacterial MutL, hMutLβ (hMLH1–hPMS1). We show that hMutLβ interacts as efficiently with hMutSα as hMutLα, and that it predominantly binds to hMutSα via hMLH1 as well.
Aim of the study: Investigation, whether water-filtered infrared-A (wIRA) irradiation during moderate bicycle ergometer endurance exercise has effects especially on local fat reduction and on weight reduction beyond the effects of ergometer exercise alone.
Methods: Randomised controlled study with 40 obese females (BMI 30-40 (median: 34.5), body weight 76-125 (median: 94.9) kg, age 20-40 (median: 35.5) years, isocaloric nutrition), 20 in the wIRA group and 20 in the control group. In both groups each participant performed 3 times per week over 4 weeks for 45 minutes bicycle ergometer endurance exercise with a constant load according to a lactate level of 2 mmol/l (aerobic endurance load, as determined before the intervention period). In the wIRA group in addition large parts of the body (including waist, hip, and thighs) were irradiated during all ergometries of the intervention period with visible light and a predominant part of water-filtered infrared-A (wIRA), using the irradiation unit “Hydrosun® 6000” with 10 wIRA radiators (Hydrosun® Medizintechnik, Müllheim, Germany, radiator type 500, 4 mm water cuvette, yellow filter, water-filtered spectrum 500-1400 nm) around a speed independent bicycle ergometer.
Main variable of interest: change of “the sum of circumferences of waist, hip, and both thighs of each patient” over the intervention period (4 weeks). Additional variables of interest: body weight, body mass index BMI, body fat percentage, fat mass, fat-free mass, water mass (analysis of body composition by tetrapolar bioimpedance analysis), assessment of an arteriosclerotic risk profile by blood investigation of variables of lipid metabolism (cholesterol, triglycerides, high density lipoproteins HDL, low density lipoproteins LDL, apolipoprotein A1, apolipoprotein B), clinical chemistry (fasting glucose, alanin-aminotransferase ALT (= glutamyl pyruvic transaminase GPT), gamma-glutamyl-transferase GGT, creatinine, albumin), endocrinology (leptin, adiponectin (= adipo Q), homocysteine, insulin). All variables were at least measured before and after the intervention period. Ergometry (ECG, blood pressure behaviour, lactate curve with power at 2, 3 and 4 mmol/l) before the intervention period. In addition: nutrition training ahead of and during the intervention period with a nutrition protocol over one week for assessment of the daily energy intake; calculation of basic metabolic rate and total energy requirement. Assessment of undesired effects.
Only methods of non-parametric statistics were used, both descriptive (median, percentiles of 25 and 75 (= interquartile range), minimum, maximum) and confirmatory (two-sided Mann-Whitney U test for unpaired samples for the only one main variable of interest). Total error probability: .05 (5%). An intention to treat analysis ITT with last observed carry forward method was used preferably (presented results) and in addition an on treatment analysis OT. Only 2 (treatment group) and 4 (control group) drop-outs occurred (mostly due to lack of time).
Results: The “sum of circumferences of waist, hip, and both thighs of each patient” decreased during the 4 weeks significantly more (p<.001) in the wIRA group than in the control group: medians and interquartile ranges: -8.0 cm (-10.5 cm/-4.1 cm) vs. -1.8 cm (-4.4 cm/0.0 cm).
As well “body weight of each patient” decreased during the 4 weeks markedly more in the wIRA group than in the control group: medians and interquartile ranges: -1.9 kg (-4.0 kg/0.0 kg) vs. 0.0 kg (-1.5 kg/+0.4 kg); median of body weight changed from 99.3 kg to 95.6 kg (wIRA) vs. 89.9 kg to 89.6 kg (control). A similar effect showed the body mass index BMI.
Blood variables of interest remained unchanged or showed some slight improvements during the treatment period, concerning most variables with no obvious differences between the two groups; insulin showed a slight trend to decrease in the wIRA group and to increase in the control group.
Undesired effects of the treatment were not seen.
Discussion: The results of the study suggest, that wIRA – during moderate bicycle ergometer endurance exercise as lipolytic stimulus – increases local lipolysis with a local fat reduction (thighs) in the otherwise bradytrophic fatty tissue. The presumably underlying mechanisms of wIRA have already been proven: wIRA acts both by thermal effects and by non-thermal effects. Thermal effects of wIRA are the generation of a therapeutic field of warmth with the increase of tissue temperature, tissue oxygen partial pressure, and tissue blood flow, and by this regional metabolism. As fatty tissue normally has a slow metabolism (bradytrophic and hypothermic tissue) with a low rate of lipolysis, wIRA can increase lipolysis in fatty tissue and the mobilized fats are burned in musculature during the ergometer exercise.
Conclusion: The results of the study indicate, that wIRA irradiation during moderate ergometer endurance exercise can be used – in combination with an appropriate nutrition – to improve body composition, especially local fat distribution, and the reduction of fat and body weight in obese persons.
RNA interference (RNAi) has emerged as a powerful tool to induce loss-of-function phenotypes by post-transcriptional silencing of gene expression. In this study we wondered whether inducible RNAi-cassettes integrated into cellular DNA possess the power to trigger neoplastic growth. For this purpose inducible RNAi vectors containing tetracycline (Tet)-responsive derivatives of the H1 promoter for the conditional expression of short hairpin RNA (shRNA) were used to target human polo-like kinase 1 (Plk1), which is overexpressed in a broad spectrum of human tumors. In the absence of doxycycline (Dox) HeLa clones expressing TetR, that carry the RNAi-cassette stably integrated, exhibited no significant alteration in Plk1 expression levels. In contrast, exposure to Dox led to marked downregulation of Plk1 mRNA to 3% and Plk1 protein to 14% in cell culture compared to mismatch shRNA/Plk1-expressing cells. As a result of Plk1 depletion cell proliferation decreased to 17%. Furthermore, for harnessing RNAi for silencing disease-related genes in vivo we transplanted inducible RNAi-HeLa cells onto nude mice. After administration of Dox knockdown of Plk1 expression was observed correlating to a significant inhibition of tumor growth. Taken together, our data revealed that genomically integrated RNAi-elements are suitable to hamper tumor growth by conditional expression of shRNA.
Natural objects provide partially redundant information to the brain through different sensory modalities. For example, voices and faces both give information about the speech content, age, and gender of a person. Thanks to this redundancy, multimodal recognition is fast, robust, and automatic. In unimodal perception, however, only part of the information about an object is available. Here, we addressed whether, even under conditions of unimodal sensory input, crossmodal neural circuits that have been shaped by previous associative learning become activated and underpin a performance benefit. We measured brain activity with functional magnetic resonance imaging before, while, and after participants learned to associate either sensory redundant stimuli, i.e. voices and faces, or arbitrary multimodal combinations, i.e. voices and written names, ring tones, and cell phones or brand names of these cell phones. After learning, participants were better at recognizing unimodal auditory voices that had been paired with faces than those paired with written names, and association of voices with faces resulted in an increased functional coupling between voice and face areas. No such effects were observed for ring tones that had been paired with cell phones or names. These findings demonstrate that brief exposure to ecologically valid and sensory redundant stimulus pairs, such as voices and faces, induces specific multisensory associations. Consistent with predictive coding theories, associative representations become thereafter available for unimodal perception and facilitate object recognition. These data suggest that for natural objects effective predictive signals can be generated across sensory systems and proceed by optimization of functional connectivity between specialized cortical sensory modules.
Background The proto-oncogene pituitary tumor-transforming gene (PTTG) has been shown to be abundantly overexpressed in a large variety of neoplasms likely promoting neo-vascularization and tumor invasiveness. In this study, we investigated a potential role for PTTG mRNA expression as a marker to evaluate the future clinical outcome of patients diagnosed with primary cancer of the head and neck. Methods Tumor samples derived from primary tumors of 89 patients suffering from a squamous cell carcinoma were analyzed for PTTG mRNA-expression and compared to corresponding unaffected tissue. Expression levels were correlated to standard clinico-pathological parameters based on a five year observation period. Results In almost all 89 tumor samples PTTG was found to be overexpressed (median fold increase: 2.1) when compared to the unaffected tissue specimens derived from the same patient. The nodal stage correlated with PTTG transcript levels with significant differences between pN0 (median expression: 1.32) and pN+ (median expression: 2.12; P = 0.016). In patients who developed a tumor recurrence we detected a significantly higher PTTG expression in primary tumors (median expression: 2.63) when compared to patients who did not develop a tumor recurrence (median expression: 1.29; P = 0.009). Since the median expression of PTTG in patients with tumor stage T1/2N0M0 that received surgery alone without tumor recurrence was 0.94 versus 3.82 in patients suffering from a tumor recurrence (P = 0.006), PTTG expression might provide a feasible mean of predicting tumor recurrence. Conclusion Elevated PTTG transcript levels might be used as a prognostic biomarker for future clinical outcome (i.e. recurrence) in primary squamous cell carcinomas of the head and neck, especially in early stages of tumor development.
Zu der grössern Zahl interessanter Urkunden, deren erste Kenntniss man dem Sammlerfleisse des Nikolaus Kindlinger verdankt, gehören einige, die das Bischofs- und Dienstmannenrecht von Köln im 12. und 13. Jahrhundert zum Gegenstand haben und dem Herausgeber bei seiner Bearbeitung des kurkölnischen Archivs bekannt geworden waren. An einem wenig zugängIichen Platze untergebracht, als urkundliche Beigabe zu einer Untersuchung, mit der sie nur lose zusammenhängen, haben sie längst verdient, der Forschung näher und bequemer gerückt zu werden. Ist dieser Vortheil nun auch schon den Hauptstücken zu Theil geworden, so hat es doch bis jetzt an einer kritischen Revision der Texte, an einer Vereinigung der Urkunden an einer Stelle und an einer zusammenhiingenden sachlichen Erläuterung derselben gefehlt. Diese Aufgaben zu lösen ist der nachstehende Aufsatz bestimmt, der in drei Abtheilungen: I. das lateinische Dienstrecht von Köln (Jura ministerialium beati Petri); II. das deutsche Dienstrecht von Köln; III. den Aufsatz : de servitio cotidiano Coloniensis archiepiscopi behandeln will.