Refine
Year of publication
Document Type
- Article (341)
- Preprint (30)
- Doctoral Thesis (24)
- Part of Periodical (7)
- Book (5)
- Contribution to a Periodical (3)
- Part of a Book (2)
- Conference Proceeding (2)
- Periodical (1)
Language
- English (383)
- German (19)
- Multiple languages (7)
- French (6)
Is part of the Bibliography
- no (415)
Keywords
- Invasive species (10)
- Biodiversity (9)
- Biogeography (9)
- taxonomy (8)
- Thailand (5)
- phylogeny (5)
- Biodiversität (4)
- Community ecology (4)
- Ecological modelling (4)
- Ecology (4)
- Evolution (4)
- Giraffa (4)
- Phylogeny (4)
- biodiversity (4)
- conservation (4)
- forensic entomology (4)
- systematics (4)
- Antarctica (3)
- Anura (3)
- Ascomycota (3)
- Asian bush mosquito (3)
- Birds (3)
- Burkina Faso (3)
- Conservation biology (3)
- Crustacea (3)
- DNA barcoding (3)
- Ecological speciation (3)
- Fungi (3)
- Hybridization (3)
- Marine biology (3)
- Mollusca (3)
- Oxidative stress (3)
- Positive selection (3)
- Southern Ocean (3)
- Species distribution modelling (3)
- West Africa (3)
- Westafrika (3)
- adaptation (3)
- biogeography (3)
- climate change (3)
- evolution (3)
- fungi (3)
- genomics (3)
- global change (3)
- host specificity (3)
- hybridization (3)
- new species (3)
- new taxa (3)
- phylogenomics (3)
- speciation (3)
- Adaptation (2)
- Animal personality (2)
- Apomixis (2)
- Asian tiger mosquito (2)
- Basidiomycetes (2)
- Basidiomycota (2)
- COI (2)
- COII (2)
- Canis lupus (2)
- Central America (2)
- Climate change (2)
- Comparative genomics (2)
- East Africa (2)
- Ecological networks (2)
- Ecosystem services (2)
- Endocrine disruption (2)
- Environmental sciences (2)
- Europe (2)
- Evolutionary ecology (2)
- Freshwater ecology (2)
- Gene flow (2)
- Genetics (2)
- German people (2)
- Human well-being (2)
- Introgression (2)
- Invertebrates (2)
- Life-history evolution (2)
- Local adaptation (2)
- Malpighiales (2)
- Mammals (2)
- NTFPs (2)
- Phylogenetics (2)
- Poecilia (2)
- Polymers (2)
- Population genomics (2)
- Potamopyrgus antipodarum (2)
- Reptilia (2)
- Savanne (2)
- Sequence alignment (2)
- Speciation (2)
- Squamata (2)
- Symbiosis (2)
- Taxonomy (2)
- Transcriptome (2)
- Trichoptera (2)
- Ursidae (2)
- WAF (2)
- bioacoustics (2)
- biospeleology (2)
- climate (2)
- community assembly (2)
- comparative genomics (2)
- dispersal (2)
- flora (2)
- flow cytometry (2)
- gene flow (2)
- genetic diversity (2)
- giraffe (2)
- land use (2)
- low-dose effects (2)
- maladaptation (2)
- molecular identification (2)
- molecular phylogenetics (2)
- mtDNA (2)
- phylogenetic network (2)
- phylogenetic signal (2)
- phylogeography (2)
- population genetics (2)
- population structure (2)
- predator recognition (2)
- retrotransposition (2)
- runs of homozygosity (2)
- smut fungi (2)
- species distribution model (2)
- species richness (2)
- temperature (2)
- whole-genome sequencing (2)
- 10 new taxa (1)
- 16S barcodes (1)
- 16S mtDNA (1)
- 17a-ethinylestradiol (1)
- 2030 Agenda (1)
- AChE inhibition (1)
- AFLP (1)
- ANOSIM (1)
- Abundance (1)
- Acacia (1)
- Acacia senegal (1)
- Acacia seyal (1)
- Aculifera (1)
- Acute toxicity (1)
- Adaptive sequence evolution (1)
- Advanced treatment technologies (1)
- Aedes aegypti (1)
- Aedes albopictus (1)
- Aerosol (1)
- Afrique de l'Ouest (1)
- Afrique de l’Ouest (1)
- Afrotheria (1)
- Agaricales (1)
- Agaricomycotina (1)
- Agroecology (1)
- Agyriales (1)
- Aichi targets (1)
- Akaike information criterion (1)
- Alburnoides bipunctatus (1)
- Allopatric diversification (1)
- Altitudinal (1)
- Ameisen (1)
- Ameisenpflanzen (1)
- American crocodile (1)
- América Central (1)
- Anamorphic fungi (1)
- Androgen (1)
- Angewandte Botanik (1)
- Angolan giraffe (1)
- Anguillicoloides crassus (1)
- Animal behavior (1)
- Animal flight (1)
- Animal navigation (1)
- Animal phylogenetics (1)
- Animal replacement (1)
- Animal wings (1)
- Anisakid nematodes (1)
- Anisakidae (1)
- Anisakidae, Pseudoterranova decipiens Komplex, Transkriptom, Verbreitung, Zoonose (1)
- Antarctic parasites (1)
- Anthropocene (1)
- Aquatic invasion (1)
- Aquatic invertebrates (1)
- Arabidopsis (1)
- Araneae (1)
- Araneomorphae (1)
- Arctic Ocean (1)
- Arctic adaptation (1)
- Arctic fox (1)
- Art (Spezies) (1)
- Artenrückgang (1)
- Assortative mating (1)
- Astaxanthin Synthase (1)
- Atakora mountain chain (1)
- Atelopus carbonerensis (1)
- Atelopus mucubajiensis (1)
- Atelopus tamaense (1)
- Australian marsupials (1)
- Autecology (1)
- Avian Clock gene (1)
- Axonal tracing (1)
- BMR (1)
- Baltic Sea (1)
- Baseline toxicity (1)
- Bathydraconinae (1)
- Batrachochytrium dentrobatidis (Bd) (1)
- Bayesian information criterion (1)
- Behavioral reaction norms (1)
- Behavioral specialization (1)
- Behavioral syndromes (1)
- Behavioral type (1)
- Benennungs-Konzepte (1)
- Benthos (1)
- Beta-diversity (1)
- Binoculars (1)
- BioFrankfurt (1)
- Biodiversity loss (1)
- Biodiversity tools and pipelines (1)
- Biogeographical representativeness (1)
- Biogeographie (1)
- Biogeography of disease (1)
- Biologging (1)
- Biological invasion (1)
- Biomonitoring (1)
- Biotest (1)
- Bird flight (1)
- Bird physiology (1)
- Bisphenol A (1)
- Blech- und Metallwarenindustrie (1)
- Blow fly (1)
- Boidae (1)
- Botany (1)
- Botswana (1)
- Bottled water (1)
- Brassicales (1)
- Bromeliads (1)
- Bufonidae (1)
- C-value (1)
- C. grani (1)
- C. radiatus (1)
- C. wailesii (1)
- CAI-1 (1)
- CCD Camera (1)
- CSO (1)
- Caddisfly (1)
- Camera trapping (1)
- Canary Current (1)
- Canis lupus familiaris (1)
- Carbohydrate active enzymes (1)
- Carychium (1)
- Catalogs (1)
- Cave animal, ecotone (1)
- Cave fish (1)
- Cave-dwelling species (1)
- Centric diatom (1)
- Cephalic sensory organs (1)
- Cerebral nerves (1)
- Chad (1)
- Chaenodraco wilsoni (1)
- Champsocephalus gunnari (1)
- Channichthyidae (1)
- Charles (1)
- Chemical communication (1)
- Chemical contamination (1)
- Chemicals of emerging concern (1)
- Chicken (1)
- Chicken embryo (1)
- China (1)
- Chiquitano Dry Forest (1)
- Chironomidae (1)
- Chironomus piger (1)
- Chironomus riparius (1)
- Chiton (1)
- Chiwondo Beds (1)
- Chocó rainforest (1)
- Chorion (1)
- Chromosome number (1)
- Chrysomya chani (1)
- Chrysomya megacephala (1)
- Cladocera (1)
- Claims on land (1)
- Climate-change ecology (1)
- Climatic habitat suitability (1)
- Coccoidea (1)
- Codon models (1)
- Coevolution (1)
- Commercial pesticide preparations (1)
- Common ragweed (1)
- Communication center (1)
- Comparative Genomics (1)
- Conservation biogeography (1)
- Contact microradiography (1)
- Contracaecum (1)
- Core effectors (1)
- Core marking (1)
- Coscinodiscus concinnus (1)
- Cretaceous warming (1)
- Croatia (1)
- Crocodylus acutus (1)
- Crude oil (1)
- Cryptic diversity (1)
- Cryptic species (1)
- Cultivation (1)
- Cybertaxonomy (1)
- DNA-Barcoding (1)
- DNA-based taxonomy (1)
- Daily flight activity (1)
- Daphnia (1)
- Darwin (1)
- Data management (1)
- Data sharing (1)
- Data standard (1)
- Data standards (1)
- Databases (1)
- Datenarchivierung (1)
- De novo assembly (1)
- Decision making (1)
- Degradation (1)
- Demersal fish (1)
- Dentin (1)
- Dentition (1)
- Depth (1)
- Deutschland (1)
- Deutschland / Abwasserverordnung (1)
- Development (1)
- Developmental biology (1)
- Diferential delay equation (1)
- Diospyro-Khayetalia senegalensis (1)
- Diospyro-Khayion senegalensis (1)
- Diptera (1)
- Direkteinleiter (1)
- Discovery (1)
- Dispersal (1)
- Dispersant (1)
- Dissemination (1)
- Distribution (1)
- Diversity (1)
- Domatien (1)
- Domestic animals (1)
- Dothideomycetes (1)
- Downy mildew (1)
- Drought (1)
- Duganella (1)
- Dysgonomonas (1)
- EBV (1)
- Earthworms (1)
- Eastern boundary upwelling ecosystem (1)
- East–west divide (1)
- Ecological niche modeling (1)
- Economic botany (1)
- Ecosystem ecology (1)
- Ecosystem effects (1)
- Ecosystem integrity (1)
- Ecosystems (1)
- Ecotoxicological status class (1)
- Ecotoxicology (1)
- Ectatosticta (1)
- Ectoparasites (1)
- Ecuador (1)
- Effector Genes (1)
- Einkommen (1)
- Eisen- (1)
- Elephants (1)
- Eltonian noise hypothesis (1)
- Emerging contaminants (ECs) (1)
- Endocrine disrupting chemical (1)
- Endogenous heat load (1)
- Energy (1)
- Energy reserves (1)
- England (1)
- Entyloma microsporum complex (1)
- Entyloma ranunculi-repentis complex (1)
- Environment (1)
- Environmental Chemistry (1)
- Environmental impact (1)
- Environmental microbiology (1)
- Environmental risk assessment (1)
- Environmental variables (1)
- Eocene; pit organs (1)
- Ephemeroptera (1)
- Ephippia (1)
- Epidemiology (1)
- Essential biodiversity variables (1)
- Estrogen receptor (1)
- Ethnobotanik (1)
- Ethnobotany (1)
- Eutheria (1)
- Evaluation matrix (1)
- Evolutionary Biology (1)
- Evolutionary ecotoxicology (1)
- Exposure (1)
- Expression analysis (1)
- Extremophile teleosts (1)
- Fate (1)
- Fatty acid metabolism (1)
- Feeding behavior (1)
- Feeding types (1)
- Fish (1)
- Fisheries (1)
- Flora (1)
- Floristische Kartierung (1)
- Flow cytometry (1)
- Flowering phenology (1)
- Flowers (1)
- Food packaging (1)
- Food processing (1)
- Food-web (1)
- Forensic entomology (1)
- Forensic science (1)
- Forensics (1)
- Forest ecology (1)
- Forest resources (1)
- Fraßschaden (1)
- Freshwater (1)
- Fruit body (1)
- Fungal evolution (1)
- Fusarium (1)
- Fusarium graminearum (1)
- GC content (1)
- GMYC (1)
- GPS collar (1)
- Gambusia (1)
- Gambusia affinis (1)
- Gambusia holbrooki (1)
- Gammarus fossarum (1)
- Gammarus roeselii (1)
- Ganoderic acids (1)
- Ganoderma lingzhi (1)
- Ganoderma lucidum (1)
- Ganodermataceae (1)
- Gastropoda (1)
- Gekkonidae (1)
- Generation time (1)
- Genetic variation (1)
- Genetic wildlife monitoring (1)
- Genome Assembly (1)
- Genomics (1)
- Geoinformationssystem (1)
- Gerlachea australis (1)
- Germany (1)
- Global (1)
- Global warming (1)
- Globaler Wandel (1)
- Glomosporiaceae (1)
- Gonad (1)
- Gonads (1)
- Gradient (1)
- Groundwater (1)
- Growth rate (1)
- Gummi-Produktivität (1)
- Gymnodraco acuticeps (1)
- Hair sampling (1)
- Hantavirus (1)
- Hatching experiments (1)
- Helicellinae (1)
- Hengduan Mountains (1)
- High-throughput screening (1)
- Himalaya (1)
- Hispaniola (1)
- Homing behavior (1)
- Hominins (1)
- Homo (1)
- Homo erectus (1)
- Homology (1)
- Host Jump (1)
- Host specificity (1)
- Humid Chaco (1)
- Hybridisation (1)
- Hybridisierung (1)
- Hydrogen sulfide (1)
- Hydromorphology (1)
- Hydrophyten (1)
- Hyperparasitism (1)
- Hypochilidae (1)
- Hyraxes (1)
- ICN (1)
- ICPP Report (1)
- Identification (1)
- Image processing (1)
- Imidacloprid (1)
- Immune function (1)
- In vitro (1)
- In vitro assays (1)
- In vitro bioassay (1)
- Indirekteinleiter (1)
- Industrieabwasser (1)
- Integrative taxonomy (1)
- Intermediate host (1)
- Interspecific competition (1)
- Interview (1)
- Intraspecific divergence (1)
- Iridaceae (1)
- Iridoideae (1)
- JIP-test (1)
- Janthinobacterium (1)
- Klima (1)
- Klimamodellierung (1)
- Klimasysteme (1)
- Klimawandel (1)
- Klimaänderung (1)
- Kosmopoliten (1)
- LINE-1 (1)
- LTER (1)
- LVSPE (1)
- Laboratory (1)
- Lake Constance (1)
- Land invasion (1)
- Landschaftsentwicklung (1)
- Latrunculia (1)
- Leaf clearing (1)
- Lepidoptera (1)
- Lesser Antilles (1)
- Life cycle strategy (1)
- Ling-zhi (1)
- Lingzhi (1)
- Localized defecation (1)
- Long-term ecological monitoring (1)
- Long‐term ecosystem research (1)
- Low-copy nuclear gene (1)
- Lure sticks (1)
- Macrophysiology (1)
- Macropus (1)
- Malawi (1)
- Malawi Rift (1)
- Mammalian genomics (1)
- Marine biodiversity (1)
- Marine ecology (1)
- Marine ecosystems (1)
- Marine food webs (1)
- Marisa cornuarietis (1)
- Masculinization (1)
- Mate choice (1)
- Maxent (1)
- McDonald-Kreitman test (1)
- Mediation (1)
- Medicinal plants (1)
- Mediterranean (1)
- Messel Formation (1)
- Metabolic critical temperatures (1)
- Meteorology (1)
- Mexico (1)
- Micro CT (1)
- Microalgae (1)
- Microbial ecology (1)
- Microbiota (1)
- Microplastic-biota interaction (1)
- Micropollutants (1)
- Microsatellites (1)
- Microthlaspi erraticum (1)
- Microtox (1)
- Middle Eastern biodiversity (1)
- MinION (1)
- Mitogenomics (1)
- Mittelmeerraum (1)
- Mixture risk assessment (1)
- Model complexity (1)
- Model of evolution (1)
- Molars (1)
- Molecular clock (1)
- Monographie (1)
- Mooré (1)
- Moraea Mill. (1)
- Morphological traits (1)
- Morphometrics (1)
- Morphometry (1)
- Mortality (1)
- Mosquitofish (1)
- Multigeneration life-cycle test (1)
- Multiple stressors (1)
- Multixenobiotic resistance activity (1)
- Museum collections (1)
- Museum samples (1)
- Mushroom (1)
- Mutualismus (1)
- Myanmar (1)
- Myotis myotis (1)
- Myrmekophytie (1)
- NOAEC (1)
- Namibia (1)
- Nanoplastics (1)
- Natricidae (1)
- Natural product synthesis (1)
- Nature (1)
- Nature reserve (1)
- Naturschutz (1)
- Nealotus tripes (1)
- Neobiota (1)
- Neogobius melanostomus (1)
- Neomeniomorpha (1)
- Neopagetopsis ionah (1)
- Neotropical fungi (1)
- Neotropics (1)
- Neozoan (1)
- Neutron Computed Tomography (1)
- Neutron Imaging (1)
- Neutron Radiography (1)
- New species (1)
- Nf2 (1)
- Niche differentiation (1)
- Nicht-Holz-Waldprodukte (1)
- Nichtholzprodukte (1)
- Nischenmodellierung (1)
- Non-invasive sampling (1)
- Non-target analysis (1)
- Noninvasive genetic sampling (1)
- Normative dimension (1)
- North Sea (1)
- Notoryctes (1)
- Nucella lapillus (1)
- Nutzwert (1)
- Oaks (1)
- Obligate biotroph (1)
- Observation (1)
- Olpidiopsis (1)
- Online Survey (1)
- Oomycetes (1)
- Oomycota (1)
- Opisthobranchia (1)
- Orangutans (1)
- Orchideen (1)
- Ostropomycetidae (1)
- Ouagadougou (1)
- Ovary (1)
- Overcompensation (1)
- Oyster farming (1)
- Oysters (1)
- PFNL (1)
- PFNLx (1)
- PTP (1)
- Pagetopsis macropterus (1)
- Palaeontology (1)
- Paleobiology (1)
- Paleoclimate (1)
- Paleontology (1)
- Panama (1)
- Panpulmonata (1)
- Panthera onca (1)
- Papierindustrie (1)
- Parachaenichthys charcoti (1)
- Paraguay (1)
- Parameter space (1)
- Paranthropus (1)
- Parasite genetics (1)
- Parasite infection (1)
- Parasites (1)
- Parasitic infection (1)
- Particulate matter (1)
- Pathosystem (1)
- Peracarida (1)
- Peracarids (1)
- Peronospora belbahrii (1)
- Peronospora lamii (1)
- Peronospora salviae-officinalis (1)
- Personality (1)
- Personality differences (1)
- Personality traits (1)
- Pertusaria (1)
- Pertusariales (1)
- Pflanzenameisen (1)
- Pflanzengeografie (1)
- Pflanzenkartierung (1)
- Pflanzensoziologie (1)
- Phenotype (1)
- Photorhabdus (1)
- Phylloscopidae (1)
- Phylloscopus (1)
- Phylogenetic analysis (1)
- Phylogenomics (1)
- Phylogeography (1)
- Physiological parameters (1)
- Phytohormones (1)
- Pigeons (1)
- Plant collections (1)
- Plant pathogen (1)
- Plastic bottles (1)
- Plastic packaging (1)
- Plecoptera (1)
- Pleistocene glaciations (1)
- Pleistocene populations (1)
- Pliocene (1)
- Pliocene–Pleistocene (1)
- Ploidy (1)
- Poecilia mexicana (1)
- Poeciliidae (1)
- Pollutants (1)
- Polyethylene terephthalate (1)
- Polypedates (1)
- Polyplacophora (1)
- Polyploidy (1)
- Polyporales (1)
- Pongo (1)
- Pontisma (1)
- Pool-Seq (1)
- Population continuity (1)
- Population dynamics (1)
- Population genetics (1)
- Population size (1)
- Positive Selection (1)
- Postglacial colonization (1)
- Potentilla (1)
- Predator–prey interaction (1)
- Predictability (1)
- Prediction (1)
- Premating isolation (1)
- Promoters (1)
- Protected Areas (1)
- Protracted speciation (1)
- Pseudochaenichthys georgianus (1)
- Pseudoterranova (1)
- Public health (1)
- Puumala virus (1)
- QSARs (1)
- RNA sequencing (1)
- Racovitzia glacialis (1)
- Rain (1)
- Ranunculaceae (1)
- Rapid evolution (1)
- Reactive oxygen species (1)
- Rearing temperature (1)
- Red fox (1)
- Reishi (1)
- Relative importance index (1)
- Relic area (1)
- Reproduction (1)
- Reproduction mode (1)
- Reproductive isolation (1)
- Reproductive potential (1)
- Research funding (1)
- Research infrastructure (1)
- Resource competition (1)
- Retrotransposon (1)
- Revision (1)
- Risk drivers (1)
- Risk metrics (risk quotients, toxic units, hazard units) (1)
- River pollution (1)
- Rodent-associated infections (1)
- Roosting place (1)
- Rosaceae (1)
- Rotenone (1)
- Ruderalarten (1)
- RxLR effectors (1)
- SINE (1)
- SNP (1)
- SNP genotyping (1)
- SPAD (1)
- STACEY (1)
- Sample preparation (1)
- Sap flow (1)
- Saprolegnia (1)
- Schmierläuse (1)
- Science–society interactions (1)
- Seasonal fluctuation (1)
- Seasonal trend decomposition (1)
- Seasons (1)
- Secondary metabolites (1)
- Segetalarten (1)
- Seicercus (1)
- Sensitivity (1)
- Sequencing (1)
- Serranía de Tabasará (1)
- Serranía de Talamanca (1)
- Sex differentiation (1)
- Sex reversal (1)
- Sexual selection (1)
- Sibling species (1)
- Simulation (1)
- Sint Maarten (1)
- Site networks (1)
- Smut Fungi (1)
- Socio-cultural factors (1)
- Socio-ecology (1)
- Solenidae (1)
- Sources (1)
- South African giraffe (1)
- Southeast Asia (1)
- Spatio-temporal patterns (1)
- Species composition (1)
- Species diversity (1)
- Species extinction (1)
- Species identification (1)
- Spinturnix myoti (1)
- Sponges (1)
- Stage-structured model (1)
- StagePop (1)
- Stewardship (1)
- Stewardship and dissemination (1)
- Structure (1)
- Substitute (1)
- Subterranean environment (1)
- Subtropical East-Atlantic (1)
- Surface waters (1)
- Suspended solids (1)
- Sustainability (1)
- Sustainability research (1)
- Svalbard (1)
- Swimming (1)
- Symbiose (1)
- Südostasien (1)
- Target screening (1)
- Tasmanian devil (1)
- Taunus (1)
- Taxonomie (1)
- Taxonomy crisis (1)
- Tchad (1)
- Teeth (1)
- Temporal variation (1)
- Territorial songs (1)
- Testis (1)
- Testosterone (1)
- The D-statistic (1)
- Thecadactylus (1)
- Thermal desorption GC–MS (1)
- Thermal pollution (1)
- Therophyten (1)
- Tiger mosquito (1)
- Tools and pipelines (1)
- Tooth wear (1)
- Tortoise beetles (1)
- Toxicity (1)
- Toxizität (1)
- Traditional medicine (1)
- Transposable elements (1)
- Trichiurus lepturus (1)
- Triterpenic acids (1)
- Tschad (1)
- Tuber aestivum (1)
- U2-OS (1)
- Umweltveränderungen (1)
- Urban ecology (1)
- Urodela (1)
- Ursus arctos (1)
- Usefulness (1)
- Ustilaginaceae (1)
- Ustilaginomycotina (1)
- Vector (1)
- Vector borne diseases (1)
- Vector mosquito (1)
- Vectorpathogen-host (1)
- Vein density (1)
- Vein networks (1)
- Vernakulärnamen (1)
- Vulpes lagopus (1)
- Vulpes vulpes (1)
- W Regional Park of Niger River (1)
- Wald als Lebensgrundlage (1)
- Wastewater treatment plant (WWTP) effluents (1)
- Water Framework Directive (1)
- Water chemistry (1)
- Water relations (1)
- Weddell Sea (1)
- Whales (1)
- White-rot (1)
- Whole Effluent Assessment (1)
- Whole-genome sequencing (1)
- Wide-scope chemical target screening (1)
- Wing morphometry (1)
- Wirbellose (1)
- Wirtspflanzen (1)
- Wolbachia (1)
- Woody communities (1)
- Xanthophyllomyces dendrorhous (1)
- Xenoestrogens (1)
- Yeast estrogen screen (1)
- Yellow fever mosquito (1)
- ZF-L (1)
- Zahn-Wellens test (1)
- Zahn-Wellens-Test (1)
- Zeigerwerte (1)
- Zoology (1)
- Zospeum (1)
- aboveground biomass (1)
- abundance (1)
- acclimation (1)
- activity sensors (1)
- air pressure (1)
- aliment sauvage (1)
- allozymes (1)
- alteración por descarga (1)
- ames fluctuation assay (1)
- amplexus (1)
- amplicon sequencing (1)
- aniline blue (1)
- ant-plants (1)
- antipredator behavior (1)
- aposematism (1)
- archeological modeling (1)
- archivage des données (1)
- asexual fungi (1)
- assembly (1)
- automated conservation assessment (1)
- avian magnetic compass (1)
- bacterial communities (1)
- bacterium-fungus interaction (1)
- barrier loci (1)
- basal oomycetes (1)
- bears (1)
- bioassay (1)
- biodiversity conservation (1)
- biodiversity education (1)
- biodiversity hotspots (1)
- biodiversity network (1)
- biodiversity networks (1)
- biodiversity research (1)
- biodiversité (1)
- biogeographic legaciese (1)
- biological archives (1)
- biological spectrum (1)
- biome (1)
- biosynthetic gene clusters (1)
- bisphenol A (1)
- blow flies (1)
- blow fly (1)
- boreotropics (1)
- bottleneck (1)
- brassicaceae (1)
- bromeliads (1)
- brown bear (1)
- caddisflies (1)
- calentamiento climático (1)
- cancer (1)
- cancer-associated fibroblasts (1)
- candidate genes (1)
- carbon-13 (1)
- cave-dwelling species (1)
- caves (1)
- chloroplast capture (1)
- chromium de novo assembly (1)
- chromosomal aberrations (1)
- chromosome number (1)
- chronosequence (1)
- citation index (1)
- climate warming (1)
- clumped isotopes (1)
- coalescence (1)
- cold tolerance (1)
- coloration (1)
- coluteocarpeae (1)
- community composition (1)
- community ecology (1)
- community-weighted mean (1)
- compaction (1)
- confocal laser scanning microscopy (1)
- connaissances écologiques traditionnelles (1)
- continental drift (1)
- control region (1)
- convergent evolution (1)
- cosmopolitan (1)
- cosmopolite (1)
- costs (1)
- cpDNA (1)
- crude oil (1)
- cryptic species (1)
- cryptochrome 1a (1)
- cuticular hydrocarbons (1)
- cytochrome oxidase (1)
- data archiving (1)
- data integration (1)
- data pruning (1)
- dataset (1)
- deadwood experiments (1)
- declinación rápida de abundancia (1)
- decline (1)
- deep-sea sponge (1)
- demographic inference (1)
- demography (1)
- denomination (1)
- dental morphology (1)
- desaturase (1)
- developmental temperature (1)
- diagenesis (1)
- diatom parasites (1)
- differentially expressed genes (1)
- digital age determination (1)
- discharge alteration (1)
- discorhabdin (1)
- disequilibrium (1)
- distribution pattern (1)
- diversidad de especies (1)
- diversity metrics (1)
- diversity of species (1)
- domatia (1)
- données écologiques (1)
- dynamic temperature (1)
- déclin d’espèce revenu (1)
- dénomination (1)
- dépendance des forêts (1)
- early fire (1)
- ecological data (1)
- ecological niche modelling (1)
- ecological speciation (1)
- ecological versatility (1)
- ecotoxicology (1)
- ectotherm (1)
- education (1)
- elongase (1)
- enamel (1)
- enamel thickness (1)
- endocrine disrupting chemicals (1)
- endocrine disruption (1)
- endocrine disruptor (1)
- environmental DNA (1)
- environmental conditions (1)
- environmental factors (1)
- environmental niche (1)
- environmental variables (1)
- epiphytism (1)
- espèces rudérales (1)
- espèces ségétales (1)
- essbare Wildpflanzen (1)
- estradiol equivalents (1)
- ethnobotanique (1)
- ethnobotany (1)
- eutroglobiont gastropod (1)
- evolutionary biology (1)
- evolutionary history (1)
- feeding (1)
- female choice (1)
- fin whales (1)
- fish bones (1)
- fitness (1)
- fixed average (1)
- flavin redox cycle (1)
- flesh flies (1)
- food quality (1)
- forensics (1)
- forest classification (1)
- forest dependency (1)
- forest functional similarity (1)
- forest management (1)
- forest tree (1)
- formicine (1)
- freeze avoidance (1)
- freshwater (1)
- freshwater ecology (1)
- freshwater ecosystems (1)
- fruit body (1)
- functional traits (1)
- gene sequence data (1)
- gene signature (1)
- gene-flow (1)
- generalized additive model (1)
- genetic differentiation (1)
- genome analysis (1)
- genomic base composition (1)
- genotoxicity (1)
- geographic distributions (1)
- geographic information system (GIS) (1)
- geomorphology (1)
- global climate change (1)
- gum productivity (1)
- habitat destruction (1)
- habitat filter (1)
- hardwood (1)
- hazard assessment (1)
- hidden speciation (1)
- high throughput (1)
- hind-casting (1)
- historical biogeography (1)
- holocarpic oomycetes (1)
- hominin adaptation (1)
- human evolution (1)
- human exposure (1)
- human pathogenic Nematoda (1)
- human–environment interaction (1)
- humidity (1)
- hybrid assembly (1)
- hybrid enrichment (1)
- hydrogen sulfide (1)
- hydrophytes (1)
- ice age refugia (1)
- ice coverage (1)
- impact factor (1)
- imposex (1)
- in vitro effects (1)
- income (1)
- incomplete lineage sorting (1)
- indicator values (1)
- indirect discharger (1)
- individual identification (1)
- industrial effluents (1)
- infrared (1)
- insect (1)
- insect abundance (1)
- internal transcribed spacer (1)
- internal transcribed spacer rDNA (1)
- interview (1)
- introgression (1)
- introgressive hybridization (1)
- invasion risk assessment (1)
- invasive fish (1)
- kangaroo (1)
- land bridges (1)
- land cover changes (1)
- land degradation (1)
- land snails (1)
- landscape structure (1)
- large carnivores (1)
- larva (1)
- larval morphology, identification (1)
- latitudinal gradient in species richness (1)
- lichen secondary metabolites (1)
- lichen-forming fungi (1)
- life cycle (1)
- life stage association (1)
- life-cycle (1)
- life-form (1)
- light-activation (1)
- livelihood (1)
- local adaptation (1)
- long-term observations (1)
- long-term research (1)
- lowland tropical forest (1)
- ländliche Einkommen (1)
- ländliche Entwicklung (1)
- macrohabitat (1)
- magnesium transporters (1)
- mammals (1)
- mammary carcinoma (1)
- management (1)
- mapping (1)
- marine invertebrates (1)
- marsupial phylogeny (1)
- marsupials (1)
- mate choice (1)
- mating type (1)
- mayflies (1)
- melanization (1)
- metabolic pathways (1)
- metamorphosis (1)
- micro-CT (1)
- microbiome (1)
- microgastropoda (1)
- microgastropods (1)
- micromorphology (1)
- micronucleus (1)
- micronucleus assay (1)
- microsatellites (1)
- microthlaspi (1)
- mineral water (1)
- mineralized tissues (1)
- mobility (1)
- molecular barcoding (1)
- molecular clock (1)
- molecular docking (1)
- molecular networking (1)
- molecular phylogeny (1)
- molecular species discrimination (1)
- molecular systematics (1)
- monitoring (1)
- mooré (1)
- morphology (1)
- multi-locus analyses (1)
- multigene analyses (1)
- multigene phylogeny (1)
- multilocus genotype (1)
- multiple stressors (1)
- multispecies coalescent (1)
- multivariate mixed model (1)
- museum collections (1)
- mutational load (1)
- mutational spectrum bias (1)
- mutualism (1)
- myrmecophytes (1)
- nature museums (1)
- near-natural (1)
- neotype (1)
- network analysis (1)
- neutron microtomography (1)
- new combinations (1)
- new primers (1)
- next generation sequencing (1)
- next-generation sequencing (1)
- nextgeneration sequencing (1)
- noccaea (1)
- noise (1)
- nomenclature (1)
- noms vernaculaires (1)
- non-independent mate choice (1)
- non-ribosomal peptide synthetases (1)
- non-timber forest products (1)
- noninvasive species (1)
- oakmoss (1)
- observaciones a largo plazo (1)
- occlusal fingerprint analysis (1)
- off-target reads (1)
- open science (1)
- orchid (1)
- orchidée (1)
- original species description (1)
- oxidative stress (1)
- oxygen-18 (1)
- palaeoecology (1)
- paleoanthropology (1)
- paleoclimate modeling (1)
- paleodiet (1)
- paleoecology (1)
- paleoenvironment modeling (1)
- paleoenvironment reconstruction (1)
- personality (1)
- phylogenetic community distance (1)
- phylogenetic conflict (1)
- phylogenetic diversity (1)
- phytodiversity (1)
- phytogeography (1)
- phytogéographie (1)
- phytosociologie (1)
- phytosociology (1)
- plant communities (1)
- plant species (1)
- plant species distributions (1)
- plant species diversity (1)
- plant-ants (1)
- plant-microbe interactions (1)
- plastic response (1)
- plastome (1)
- pleomorphic fungi (1)
- ploidy (1)
- polyketide synthases (1)
- polyploidy (1)
- polytomy (1)
- population density (1)
- population genomics (1)
- population growth (1)
- populations rurales pauvres (1)
- portable fluorimeter (1)
- positive selection (1)
- postglacial recolonization (1)
- potential invasion area (1)
- predator avoidance (1)
- primate evolution (1)
- prioritization (1)
- produits forestiers non ligneux (1)
- prosobranchia (1)
- protected areas (1)
- protected names (1)
- public awareness (1)
- qPCR (1)
- quality control (1)
- quantitative shell variation (1)
- quorum sensing (1)
- radical pairs (1)
- random genetic drift (1)
- range size (1)
- rapid abundance decline (1)
- razor clams (1)
- re-description (1)
- reassembly (1)
- recolonization (1)
- red algae (1)
- refined fuels (1)
- regurgitation pellets (1)
- reintroduction (1)
- rejected names (1)
- relationships (1)
- relevés (1)
- repeatability (1)
- reproductive isolation (1)
- reproductive toxicity (1)
- reserve substance (1)
- resilience (1)
- resistance (1)
- ressources de base (1)
- retrotransposons (1)
- revenu rural (1)
- review (1)
- ruderal species (1)
- rural income (1)
- rural poor (1)
- salinity (1)
- savane (1)
- savanna ecosystem (1)
- scientific communication (1)
- sea-ice meiofauna (1)
- seafood allergy (1)
- segetal species (1)
- sex ratio (1)
- sexual ornament (1)
- shell eyes (1)
- shell variability (1)
- short read assembly (1)
- six new taxa (1)
- smut fung (1)
- soil (1)
- soil VOCs (1)
- soil bacteria communities (1)
- soil degradation (1)
- soil fungal communities (1)
- soil temperature (1)
- song evolution (1)
- southern Africa (1)
- spatio-temporal analysis (1)
- spatio-temporal evolution (1)
- species complex (1)
- species decline (1)
- species delineation (1)
- species discrimination (1)
- species distribution modelling (1)
- species distribution models (1)
- species groups (1)
- species inventory (1)
- species tree (1)
- specific average (1)
- split decomposition (1)
- stability (1)
- stoneflies (1)
- stream macroinvertebrates (1)
- structure et productivité de gomme (1)
- subgenera (1)
- substituts (1)
- subterranean land snail (1)
- sudano-sahelian zone (1)
- sudano-sahelien zone (1)
- sudano-sahelische Zone (1)
- superfemale (1)
- sustainability (1)
- systematic diversity (1)
- systems knowledge (1)
- taphonom (1)
- taphonomy (1)
- target knowledge (1)
- taxon sampling (1)
- taxonomic impediment (1)
- taxonomía (1)
- temperature adaptation (1)
- terpene synthases (1)
- the Weddell Sea (1)
- therophytes (1)
- thlaspi (1)
- threats (1)
- thérophytes (1)
- traditional ecological knowledge (1)
- traditionelles ökologisches Wissen (1)
- transcription factor (1)
- transcriptional profiling (1)
- transcriptome (1)
- transcriptome analysis (1)
- transcriptomics (1)
- transdisciplinarity (1)
- transfer (1)
- transformation knowledge (1)
- transposable element (1)
- transposable elements (1)
- tree moss (1)
- trees (1)
- tributyltin (1)
- tropical Africa (1)
- tropical forests (1)
- tropical fungi (1)
- trypan blue (1)
- type species (1)
- under-ice fauna (1)
- unit nomenclature (1)
- urban park (1)
- urbanity gradient (1)
- urbanization (1)
- use value (1)
- valeur d'usage (1)
- valeurs indicatrices (1)
- variable selection (1)
- vernacular names (1)
- violacein (1)
- vocalización (1)
- vocalization (1)
- volatile organic compounds (1)
- voucherless taxa (1)
- water parameter (1)
- wax layer (1)
- whaling (1)
- wild food (1)
- winter survival (1)
- wood-inhabiting fungi (1)
- woody plant community (1)
- worm-mollusc (1)
- xenoestrogen (1)
- zone sahélo-soudanienne (1)
- zooplankton (1)
- ökologische Daten (1)
Institute
- Institut für Ökologie, Evolution und Diversität (415) (remove)
Neanderthal diet has been on the spotlight of paleoanthropological research for many years. The majority of studies that tried to reconstruct the diet of Neanderthals were based on the analysis of zooarchaeological remains, stable isotopes, dental calculus and dental microwear patterns. In the past few years, there have been a few studies that linked dental macrowear patterns of Neanderthals and modern humans to diet and cultural habits. However, they mostly focused on maxillary molars. Although mandibular molars have been widely used in microwear dietary research, little is known about their usage at the macroscopic scale to detect information about human subsistence strategies. In this study, we compare the macrowear patterns of Neanderthal (NEA), fossil Homo sapiens (FHS), modern hunter-gatherers (MHG), pastoralists, early farmers and Australian Aborigines from Yuendumu mandibular molars in order to assess their utility in collecting any possible information about dietary and cultural habits among diverse human groups. We use the occlusal fingerprint analysis method, a quantitative digital approach that has been successfully employed to reconstruct the diet of living non-human primates and past human populations. Our results show macrowear pattern differences between meat-eater MHG and EF groups. Moreover, while we did not find eco-geographical differences in the macrowear patterns of the fossil sample, we found statistically significant differences between NEA and FHS inhabiting steppe/coniferous forest. This latter result could be associated with the use of distinct technological complexes in these two species, which ultimately could have allowed modern humans to exploit natural resources in a different way compared to NEA.
Highlights
• 153 chemicals of emerging concern detected in complex multi-component mixtures.
• 108 possible mixture risk assessment scenarios were investigated.
• Non-detects, QSARs, and experimental ecotoxicological data were integrated for risk assessment.
• 8 chemicals were the main risk drivers in at least one site across the River Aconcagua basin.
Abstract
Environmental risk assessments strategies that account for the complexity of exposures are needed in order to evaluate the toxic pressure of emerging chemicals, which also provide suggestions for risk mitigation and management, if necessary. Currently, most studies on the co-occurrence and environmental impacts of chemicals of emerging concern (CECs) are conducted in countries of the Global North, leaving massive knowledge gaps in countries of the Global South.
In this study, we implement a multi-scenario risk assessment strategy to improve the assessment of both the exposure and hazard components in the chemical risk assessment process. Our strategy incorporates a systematic consideration and weighting of CECs that were not detected, as well as an evaluation of the uncertainties associated with Quantitative Structure-Activity Relationships (QSARs) predictions for chronic ecotoxicity. Furthermore, we present a novel approach to identifying mixture risk drivers. To expand our knowledge beyond well-studied aquatic ecosystems, we applied this multi-scenario strategy to the River Aconcagua basin of Central Chile. The analysis revealed that the concentrations of CECs exceeded acceptable risk thresholds for selected organism groups and the most vulnerable taxonomic groups. Streams flowing through agricultural areas and sites near the river mouth exhibited the highest risks. Notably, the eight risk drivers among the 153 co-occurring chemicals accounted for 66–92 % of the observed risks in the river basin. Six of them are pesticides and pharmaceuticals, chemical classes known for their high biological activity in specific target organisms.
From hunting and foraging to clearing land for agriculture, humans modify forest biodiversity, landscapes, and climate. Forests constantly undergo disturbance–recovery dynamics and understanding them is a major objective of ecologists and conservationists. Chronosequences are a useful tool for understanding global restoration efforts. They represent a space-for-time substitution approach suited for the quantification of the resistance of ecosystem properties to withstand disturbance and the resilience of these properties until reaching pre-disturbance levels. Here we introduce a newly established chronosequence with 62 plots (50 ⍰ 50 m) in active cacao plantations and pastures, early and late regeneration, and mature old-growth forests, across a 200 km2 area in the extremely wet Chocó rainforest. Our chronosequence covers by far the largest total area of plots compared to others in the Neotropics. Plots ranged from 159–615 masl in a forested landscape with 74 ± 2.8 % forest cover within a 1-km radius including substantial old-growth forest cover. Land-use legacy and regeneration time were not confounded by elevation. We tested how six forest structure variables (maximum tree height and DBH, basal area, number of stems, vertical vegetation heterogeneity, and light availability), aboveground biomass (AGB), and rarefied tree species richness change along our chronosequence. Forest structure variables, AGB, and tree species richness increased with regeneration time and are predicted to reach similar levels to those in old-growth forests after ca. 30–116, 202, and 108 yrs, respectively. Compared to previous work in the Neotropics, old-growth forests in Canandé accumulate high AGB that takes one of the largest time spans reported until total recovery. Our chronosequence comprises one of the largest tree species pools, covers the largest total area of regenerating and old-growth forests, and has higher forest cover than other Neotropical chronosequences. Hence, our chronosequence can be used to determine the time for recovery and stability (resistance and resilience) of different taxa and ecosystem functions, including species interaction networks. This integrative effort will ultimately help to understand how one of the most diverse forests on the planet recovers from large-scale disturbances.
Highlights
• BaP exposure increases the mutation rate of C. riparius.
• BaP exposure is detrimental for the fitness and the population dynamics of C. riparius.
• Multi-generational studies are essential to assess evolutionary implications of anthropogenic substances on biodiversity.
Abstract
The release of polycyclic aromatic hydrocarbons (PAHs) into the environment is posing a threat to ecosystems and human health. Benzo(a)pyrene (BaP) is considered a biomarker of PAH exposure and is classified as a Group 1 carcinogen. However, it was not known whether BaP is mutagenic, i.e. induces inherited germline mutations. In this study, we used a recently established method, which combines short-term mutation accumulation lines (MAL) with whole genome sequencing (WGS) to assess mutagenicity in the non-biting midge Chironomus riparius. The mutagenicity analysis was supplemented by an evaluation of the development of population fitness in three successive generations in the case of chronic exposure to BaP at a high concentration (100 μg/L). In addition, the level of ROS-induced oxidative stress was examined in vivo. Exposure to the higher BaP concentration led to an increase in germline mutations relative to the control, while the lower concentration showed no mentionable effect. Against expectations, BaP exposure decreased ROS-level compared to the control and is thus probably not responsible for the increased mutation rate. Likewise, the higher BaP concentration decreased fitness measured as population growth rate per day (PGR) significantly over all generations, without signs of rapid evolutionary adaptations. Our results thus highlighted that high BaP exposure may influence the evolutionary trajectory of organisms.
Seed harvesting from wild plant populations is key for ecological restoration, but may threaten the persistence of source populations. Consequently, several countries have set guidelines limiting the proportions of harvestable seeds. Here, we use high-resolution data from 298 plant species to model the demographic consequences of seed harvesting. We find that the current guidelines only protect some species, but are insufficient or overly restrictive for others. We show that the maximum possible fraction of seed harvesting is strongly associated with harvesting frequency and generation time of the target species, ranging from 100% in long-lived species to <1% in the most annuals. Our results provide quantitative basis to guide seed harvesting legislation based on species’ generation time and harvesting regime.
The European bison was saved from the brink of extinction due to considerable conservation efforts since the early 20th century. The current global population of > 9,500 individuals is the result of successful ex situ breeding based on a stock of only 12 founders, resulting in an extremely low level of genetic variability. Due to the low allelic diversity, traditional molecular tools, such as microsatellites, fail to provide sufficient resolution for accurate genetic assessments in European bison, let alone from non-invasive samples. Here, we present a SNP panel for accurate high-resolution genotyping of European bison, which is suitable for a wide variety of sample types. The panel accommodates 96 markers allowing for individual and parental assignment, sex determination, breeding line discrimination, and cross-species detection. Two applications were shown to be utilisable in further Bos species with potential conservation significance. The new SNP panel will allow to tackle crucial tasks in European bison conservation, including the genetic monitoring of reintroduced populations, and a molecular assessment of pedigree data documented in the world’s first studbook of a threatened species.
Background: In the speciation continuum, the strength of reproductive isolation varies, and species boundaries are blurred by gene flow. Interbreeding among giraffe (Giraffa spp.) in captivity is known, and anecdotal reports of natural hybrids exist. In Kenya, Nubian (G. camelopardalis camelopardalis), reticulated (G. reticulata), and Masai giraffe sensu stricto (G. tippelskirchi tippelskirchi) are parapatric, and thus, the country might be a melting pot for these taxa. We analyzed 128 genomes of wild giraffe, 113 newly sequenced, representing these three taxa.
Results: We found varying levels of Nubian ancestry in 13 reticulated giraffe sampled across the Laikipia Plateau most likely reflecting historical gene flow between these two lineages. Although comparatively weaker signs of ancestral gene flow and potential mitochondrial introgression from reticulated into Masai giraffe were also detected, estimated admixture levels between these two lineages are minimal. Importantly, contemporary gene flow between East African giraffe lineages was not statistically significant. Effective population sizes have declined since the Late Pleistocene, more severely for Nubian and reticulated giraffe.
Conclusions: Despite historically hybridizing, these three giraffe lineages have maintained their overall genomic integrity suggesting effective reproductive isolation, consistent with the previous classification of giraffe into four species.
Highlights
• Genomes for all five Natrix species, two represented by two distinct subspecies each, were sequenced.
• Two genomes were de-novo assembled to their 1.7 Gb length with a contig N50 of 4.6 Mbp and 1.5 Mbp.
• Evidence for interspecific hybridization, both between allopatric and widely sympatric species.
• Fossil-calibrated molecular clock using genomes indicates that species are ancient several million-year-old lineages.
• Our findings imply that speciation took place despite continued gene flow.
Abstract
Understanding speciation is one of the cornerstones of biological diversity research. Currently, speciation is often understood as a continuous process of divergence that continues until genetic or other incompatibilities minimize or prevent interbreeding. The Palearctic snake genus Natrix is an ideal group to study speciation, as it comprises taxa representing distinct stages of the speciation process, ranging from widely interbreeding parapatric taxa through parapatric species with very limited gene flow in narrow hybrid zones to widely sympatric species. To understand the evolution of reproductive isolation through time, we have sequenced the genomes of all five species within this genus and two additional subspecies. We used both long-read and short-read methods to sequence and de-novo-assemble two high-quality genomes (Natrix h. helvetica, Natrix n. natrix) to their 1.7 Gb length with a contig N50 of 4.6 Mbp and 1.5 Mbp, respectively, and used these as references to assemble the remaining short-read-based genomes. Our phylogenomic analyses yielded a well-supported dated phylogeny and evidence for a surprisingly complex history of interspecific gene flow, including between widely sympatric species. Furthermore, evidence for gene flow was also found for currently allopatric species pairs. Genetic exchange among these well-defined, distinct, and several million-year-old reptile species emphasizes that speciation and maintenance of species distinctness can occur despite continued genetic exchange.
The snake pipefish, Entelurus aequoreus (Linnaeus, 1758), is a slender, up to 60 cm long, northern Atlantic fish that dwells in open seagrass habitats and has recently expanded its distribution range. The snake pipefish is part of the family Syngnathidae (seahorses and pipefish) that has undergone several characteristic morphological changes, such as loss of pelvic fins and elongated snout. Here, we present a highly contiguous, near chromosome-scale genome of the snake pipefish assembled as part of a university master’s course. The final assembly has a length of 1.6 Gbp in 7,391 scaffolds, a scaffold and contig N50 of 62.3 Mbp and 45.0 Mbp and L50 of 12 and 14, respectively. The largest 28 scaffolds (>21 Mbp) span 89.7% of the assembly length. A BUSCO completeness score of 94.1% and a mapping rate above 98% suggest a high assembly completeness. Repetitive elements cover 74.93% of the genome, one of the highest proportions so far identified in vertebrate genomes. Demographic modeling using the PSMC framework indicates a peak in effective population size (50 – 100 kya) during the last interglacial period and suggests that the species might largely benefit from warmer water conditions, as seen today. Our updated snake pipefish assembly forms an important foundation for further analysis of the morphological and molecular changes unique to the family Syngnathidae.
Methods using environmental DNA to explore and analyze biodiversity from previously unexplored habitats and ecosystems have become increasingly popular in recent years. This is particularly due to the potential reduction in necessary taxonomic expertise, the opportunity to assess microorganismal communities, and decreased time investments required to cover large spatial extents. In forests, the surface of tree bark is an important habitat for epiphytic diversity. Because of the large surface area rich in micro-niches, the seasonal stability of the substrate, and the longevity of trees, tree bark surfaces provide an ideal habitat for many species. Yet, we lack a comprehensive understanding of their communities and the environmental drivers behind the community assembly. These missing links hinder the exploration of the forest microbiome as a whole and limits our understanding of functions of a large forest habitat and its connections to other forest microbiomes. With a holistic eDNA metabarcoding approach, encompassing samples of three major taxonomic groups (e.g. bacteria, fungi, and green algae), as well as simultaneous collections from multiple forest habitats we can contribute to closing these gaps and increase our knowledge of the forest microbiome.
My dissertation is set within the framework of the Biodiversity Exploratories and was conducted in four parts: I. the establishment of an eDNA metabarcoding workflow to reveal the local diversity of the bark surface microbiome; II. the upscaling of the method to large geographic and environmental gradients to uncover the drivers of the microbiome; III. the integration of soil and bark samples to investigate compositional differences in two important forest habitats; IV. the evaluation of eDNA metabarcoding as a tool for biodiversity assessments of lichen diversity in forests.
In the first part, I developed a simple, cost-effective and fast sampling strategy to acquire eDNA samples from the bark of trees in forest ecosystems. Using readily available medical-specimen-collection swabs I sampled bark surfaces of individual trees in Central German forests and used metabarcoding to amplify marker genes of green algae, fungi and bacteria. From the sequencing reads I calculated the first diversity estimates of the major organismal groups of bark surface microbiomes from Central European forests. Overall the methodology produced reliable results, allowing for an expanded sampling in the second part.
In the second part of the dissertation, I expanded the sampling based on the results of part one. I collected bark surface samples from the three regions of the Biodiversity Exploratories covering large spatial and environmental gradients representative for Central European forests. The collection included composite samples from 150 plots and over 750 trees. Utilizing measurements of climatic and forest structure variables provided by the Biodiversity Exploratories, as well as my own community data, I identified the biotic and abiotic drivers behind alpha and beta diversity of the bark surface microbiome.
In the third part, I studied the differences between the bark surface as an unexplored and the soil as an example of a well characterized forest microbiome. Using only the fungal part of the large sampling campaign and soil samples obtained from the same plots at the same time, I assessed the commonalities and differences of the micro-communities of these distinct forest niches. Furthermore, I included two coniferous and one deciduous tree species to examine, if the effect of tree species, previously shown for soil microbiomes, also holds true for the bark surface.
In the last part of my dissertation, I used eDNA in a more applied way as a tool in biodiversity assessments of lichenized fungi. I compared the results from eDNA metabarcoding to an expert floristic mapping conducted in the same plots in 2007/2008. I assigned functional guilds to the fungal taxa obtained in the large sampling campaign and used a subset that was assigned as lichenized fungi.
In conclusion, I showed that eDNA metabarcoding is a valuable tool to reveal the unknown diversity of microorganisms in forest ecosystems. In particular, my results advance our understanding of the bark surface microbiome, an underexplored habitat within forests. The tightly linked interactions of the three major microbial groups underline that studies need to take holistic approaches across multiple taxonomic groups to deepen our understanding of processes governing the assembly of microbiomes. Results from my dissertation may serve as a foundation to inform hypotheses addressing the functions of forest microbiomes. The massive diversity data collected may also contribute to closing the gap in our understanding of macro-organisms and micro-organisms with respect to diversity distributions and patterns of richness, and serve as a baseline for predictions of biodiversity responses under future anthropogenic change.
Nitrogen pollution is a major constituent of global change, threatening local biodiversity, ecosystem services, and causing serious environmental damage. Specifically, in areas with heavy agricultural soil-use, excessive use of nitrogen fertilizer pollutes the groundwaters with nitrates, but also with ammonia and nitrites. Freshwater fish and other aquatic fauna are especially vulnerable to nitrites, which can cause massive mortalities at even low concentrations < 0.1 mg/l NO2- - N. Adaptation of fish to environments with relatively high concentrations of chemicals has occurred throughout the history of life, although contemporary evolution acts at a much more rapid pace. The growing use of land for mass agriculture and livestock industries in the last 50 years in the US has dramatically increased the nutrient loading into the surface and groundwaters. Nitrite poses a serious threat for freshwater fauna as it is rapidly up taken and disturbs ion homeostasis and blood gas transport in fish. In this study, we evaluated, by means of a laboratory experiment, the tolerance of fish to nitrite using six different populations of wild eastern mosquitofish (Gambusia holbrooki) from two regions, North FL and NC, and with different background nitrogen pollution histories. Mosquitofish females were exposed to nitrite in the lab, to either < 0.005 mg/l NO2- (control) or 0.3 mg/l NO2- for ten days and we assessed at the end of the exposure period their blood O2 transport capacity by measuring the concentration of four different types of hemoglobin, their total hematocrit, and their respiratory rates. Preliminary results show significant varying patterns in the response of the exposed fish, depending on the population source, as evidenced by their respiratory rates and the blood erythrocyte counts. Mortality was very low, and hemoglobin profiles indicate high tolerance of G. holbrooki to nitrite contamination – a factor supporting their invasion success in agriculturally dominated regions around the world
Background: In the speciation continuum the strength of reproductive isolation varies, and species boundaries are blurred by gene flow. Interbreeding among giraffe (Giraffa spp.) in captivity is known and anecdotal reports of natural hybrids exist. In Kenya, Nubian (G. camelopardalis camelopardalis), reticulated (G. reticulata), and Masai giraffe sensu stricto (G. tippelskirchi tippelskirchi) are parapatric, and thus the country might be a melting pot for these taxa. We analyzed 128 genomes of wild giraffe, 113 newly sequenced, representing these three taxa.
Results: We found varying levels of Nubian ancestry in 13 reticulated giraffe sampled across the Laikipia Plateau most likely reflecting historical gene flow between these two lineages. Although comparatively weaker signs of ancestral gene flow and potential mitochondrial introgression from reticulated into Masai giraffe were also detected, estimated admixture levels between these two lineages are minimal. Importantly, contemporary gene flow between East African giraffe lineages was not statistically significant. Effective population sizes have declined since the Late Pleistocene, more severely for Nubian and reticulated giraffe.
Conclusions: Despite historically hybridizing, these three giraffe lineages have maintained their overall genomic integrity suggesting effective reproductive isolation, consistent with the previous classification of giraffe into four species.
Chemical pollution caused by synthetic organic chemicals at low concentrations in the environment poses a growing threat to the ecological status of aquatic ecosystems. These chemicals are regularly released into surface waters through both treated and untreated effluents from wastewater treatment plants (WWTPs), agricultural runoff, and industrial discharges. Consequently, they accumulate in surface waters, distribute amongst environmental compartments according to their physicochemical properties, and cause adverse effects on aquatic organisms. Unfortunately, there is a lack of data regarding the occurrence of synthetic organic chemicals, henceforth micropollutants, in South American freshwater ecosystems, especially in Chile.
To address this research gap, we present a comprehensive dataset comprising concentrations of 153 emerging chemicals, including pesticides, pharmaceutical and personal care products (PPCPs), surfactants, and industrial chemicals. These chemicals were found to co-occur in surface waters within Central Chile, specifically in the River Aconcagua Basin. Our sampling strategy involved collecting surface water samples from streams and rivers with diverse land uses, such as agriculture, urban areas, and natural reserves. For sample extraction, we employed an on-site large-volume solid phase extraction (LVSPE) device. The resulting environmental extracts were then subjected to wide-scope chemical target screening using gas chromatography and liquid chromatography high-resolution mass spectrometry (GC- and LCsingle bondHRMS).
The dataset we present holds significant value in assessing the chemical status of water bodies. It enables comparative analysis of pollution fingerprints associated with emerging chemicals across different freshwater systems. Moreover, the data can be reused for environmental risk assessment studies. Its utilisation will contribute to a better understanding of the impact and extent of chemical pollution in aquatic ecosystems, facilitating the development of effective mitigation strategies.
Genome mining as a biotechnological tool for the discovery of novel biosynthetic genes in lichens
(2022)
The ever-increasing demand for novel drugs highlights the need for bioprospecting unexplored taxa for their biosynthetic potential. Lichen-forming fungi (LFF) are a rich source of natural products but their implementation in pharmaceutical industry is limited, mostly because the genes corresponding to a majority of their natural products is unknown. Furthermore, it is not known to what extent these genes encode structurally novel molecules. Advance in next-generation sequencing technologies has expanded the range of organisms that could be exploited for their biosynthetic potential. In this study, we mine the genomes of nine lichen-forming fungal species of the genus Umbilicaria for biosynthetic genes, and categorize the BGCs as “associated product structurally known”, and “associated product putatively novel”. We found that about 25-30% of the biosynthetic genes are divergent when compared to the global database of BGCs comprising of 1,200,000 characterized biosynthetic genes from planta, bacteria and fungi. Out of 217 total BGCs, 43 were only distantly related to known BGCs, suggesting they encode structurally and functionally unknown natural products. Clusters encoding the putatively novel metabolic diversity comprise PKSs (30), NRPSs (12) and terpenes (1). Our study emphasizes the utility of genomic data in bioprospecting microorganisms for their biosynthetic potential and in advancing the industrial application of unexplored taxa. We highlight the untapped structural metabolic diversity encoded in the lichenized fungal genomes. To the best of our knowledge, this is the first investigation identifying genes coding for NPs with potentially novel therapeutic properties in LFF.
Intraspecific genomic variability affects a species’ adaptive potential towards climatic conditions. Variation in gene content across populations and environments may point at genomic adaptations to specific environments. The lichen symbiosis, a stable association of fungal and photobiont partners, offers an excellent system to study environmentally driven gene content variation. Many species have remarkable environmental tolerances, and often form populations in different climate zones. Here we combine comparative and population genomics to assess the presence and absence of genes in high elevation and low elevation genomes of two lichenized fungi of the genus Umbilicaria. The two species have non-overlapping ranges, but occupy similar climatic niches in North America (U. phaea) and Europe (U. pustulata): high elevation populations are located in the cold temperate zone and low elevation populations in the Mediterranean zone. We assessed gene content variation along replicated elevation gradients in each of the two species, based on a total of 2050 individuals across 26 populations. Specifically, we assessed shared orthologs across species within the same climate zone, and tracked which genes increase or decrease in abundance within populations along elevation. In total, we found 16 orthogroups with shared orthologous genes in genomes at low elevation and 13 at high elevation. Coverage analysis revealed one ortholog that is exclusive to genomes at low elevation. Conserved domain search revealed domains common to the protein kinases (PKs) superfamily. We traced the discovered ortholog in populations along five replicated elevation gradients on both continents. The protein kinase gene linearly declined in abundance with increasing elevation, and was absent in the highest populations. We consider the parallel loss of an ortholog in two species and in two geographic settings a rare find, and a step forward in understanding the genomic underpinnings of climatic tolerances in lichenized fungi. In addition, the tracking of gene content variation provides a widely applicable framework for retrieving biogeographical determinants of gene presence/absence patterns. Our work provides insights into gene content variation of lichenized fungi in relation to climatic gradients, suggesting a new research direction with implications for understanding evolutionary trajectories of complex symbioses in relation to climatic change.
Climate change affects ecosystems worldwide and is threatening biodiversity. Insects, as ectotherm organisms, are strongly dependent on the thermal environment. Yet, little is known about the effects of summer heat and drought on insect diversity. In the Mediterranean climate zone, a region strongly affected by climate change, hot summers might have severe effects on insect communities. Especially the larval stage might be sensitive to thermal variation, as larvae—compared to other life stages—cannot avoid hot temperatures and drought by dormancy. Here we ask, whether inter-annual fluctuations in Mediterranean moth diversity can be explained by temperature (TLarv) and precipitation during larval development (HLarv). To address our question, we analyzed moth communities of a Mediterranean coastal forest during the last 20 years. For species with summer-developing larvae, species richness was significantly negatively correlated with TLarv, while the community composition was affected by both, TLarv and HLarv. Therefore, summer-developing larvae seem particularly sensitive to climate change, as hot summers might exceed the larval temperature optima and drought reduces food plant quality. Increasing frequency and severity of temperature and drought extremes due to climate change, therefore, might amplify insect decline in the future.
A risk based assessment approach for chemical mixtures from wastewater treatment plant effluents
(2022)
In this study, 56 effluent samples from 52 European wastewater treatment plants (WWTPs) were investigated for the occurrence of 499 emerging chemicals (ECs) and their associated potential risks to the environment. The two main objectives were (i) to extend our knowledge on chemicals occurring in treated wastewater, and (ii) to identify and prioritize compounds of concern based on three different risk assessment approaches for the identification of consensus mixture risk drivers of concern. Approaches include (i) PNEC and EQS-based regulatory risk quotients (RQs), (ii) species sensitivity distribution (SSD)-based hazard units (HUs) and (iii) toxic units (TUs) for three biological quality elements (BQEs) algae, crustacean, and fish.
For this purpose, solid-phase extracts were analysed with wide-scope chemical target screening via liquid chromatography high-resolution mass spectrometry (LC-HRMS), resulting in 366 detected compounds, with concentrations ranging from < 1 ng/L to > 100 µg/L. The detected chemicals were categorized with respect to critical information relevant for risk assessment and management prioritization including: (1) frequency of occurrence, (2) measured concentrations, (3) use groups, (4) persistence & bioaccumulation, and (5) modes of action. A comprehensive assessment using RQ, HU and TU indicated exceedance of risk thresholds for the majority of effluents with RQ being the most sensitive metric. In total, 299 out of the 366 compounds were identified as mixture risk contributors in one of the approaches, while 32 chemicals were established as consensus mixture risk contributors of high concern, including a high percentage (66%) of pesticides and biocides. For samples which have passed an advanced treatment using ozonation or activated carbon (AC), consistently much lower risks were estimated.
Im Rahmen dieser Dissertation wurden unterschiedliche Aspekte der Verbreitung der Vertreter des Pseudoterranova decipiens Komplexes betrachtet und Fragestellungen zur Ökologie und Humanpathogenität der Parasiten bearbeitet. Sie basiert auf drei (ISI-) Fachartikeln, in denen die Nutzung von Fischparasitengemeinschaften als ökologische Indikatoren für entlegene Ökosysteme des Südpolarmeeres (I), die Modellierung geeigneter Verbreitungsgebiete für Arten mit geringen Vorkommensdaten am Beispiel des P. decipiens Komplexes (II) und das Vorkommen potentiell humanpathogener P. bulbosa in unterschiedlichen Mikrohabitaten in Atlantischem Kabeljau (III) thematisiert wurde.
Die Parasitengemeinschaften der in Studie I untersuchten, nahverwandten Antarktisdorsche (Nototheniinae) Nototheniops larseni (n=40), N. nudifrons (n=40) und Lepidonotothen squamifrons (n=49) unterschieden sich hauptsächlich hinsichtlich seltener Parasitenarten. Pseudoterranova decipiens E zählte zu den häufigsten Parasiten der drei betrachteten Wirtsarten. Die Analyse der Wirtsspektren der auf Artebene bestimmten Parasiten zeigte eine geringe Spezifität antarktischer Fischparasiten im Larven- (z.B. Pseudoterranova decipiens E) und Adultstadium (z.B. Elytrophalloides oatesi). Für eine Nutzung als Bioindikatoren ergibt sich die Empfehlung, nicht auf einzelne Parasitenarten, sondern die Zusammensetzung von Parasitenfaunen zurückzugreifen und Parameter wie Abundanz oder Intensität zu berücksichtigen. Vergleiche mit Literaturdaten legten nahe, dass ein Studiendesign, das den periodischen Vergleich der Parasitierungsmuster von Nototheniinae ermöglichen soll, Standorteffekte berücksichtigen sollte. Da es sich bei der Probennahme demersaler Fische um ein aufwändiges und einschneidendes Verfahren handelt, sollten alternative Samplingmethoden vorangetrieben und eine Datenbasis dafür geschaffen werden.
Um die Belastung von Speisefischen mit potentiell humanpathogenen Parasiten in bestimmten Fanggebieten abzuschätzen, kann anhand von Vorkommens- und Umweltdaten mittels statistischer Modelle die Habitateignung für den Parasiten bestimmt werden. Eine Voraussetzung für eine verlässliche Modellierung bilden die Wahl eines geeigneten Algorithmus und die Qualität der Eingangsdaten. Für die Modellierung geeigneter Verbreitungsgebiete für die sechs Arten des P. decipiens Komplexes wurde im Rahmen von Studie II erstmalig ein biotischer Deskriptor herangezogen. Dem Ansatz lag die Annahme zugrunde, dass das Vorkommen geeigneter Endwirte der entscheidende, limitierende Faktor für die Verbreitung eines Parasiten ist, da nur so der Lebenszyklus geschlossen werden kann. Als Hypothesentest dienten Vergleiche der ökologischen Nischen von Parasiten und ihren spezifischen Endwirten im Nischenraum. Anhand der Endwirtdistanz wurde eine Verbesserung der Modellierungsergebnisse mit MaxEnt, gegenüber der ausschließlich auf abiotischen Prädiktoren basierenden Modellierung, für alle Pseudoterranova Arten, insbesondere jene mit einer geringen Anzahl Fundpunkte, erzielt. Grundsätzlich ist der Ansatz auf marine Parasitenarten, deren spezifische Endwirte verlässliche Vorkommensdaten aufweisen, übertragbar. Die Methode stellt jedoch keinen Ersatz für die Erhebung von Vorkommensdaten dar, weshalb die genetische Bestimmung schwer zu identifizierender Taxa sowie die Angabe von Metadaten in jeder parasitologischen Studie obligatorisch sein sollten.
Die Verteilung potentiell humanpathogener Parasitenstadien in für den menschlichen Verzehr vorgesehenen Fischen kann ein entscheidender Faktor für die Übertragung sein. Im Rahmen von Studie III wurde mit dem Referenztranskriptom von P. bulbosa das erste Transkriptom für eine Art den P. decipiens Komplexes erstellt. Anhand einer differentiellen Genexpressionsanalyse wurde untersucht, was die Verteilung der Parasiten auf unterschiedliche Mikrohabitate beeinflusst haben könnte. Dabei wurden siebzig differentiell exprimierte Gene identifiziert, die in aus Leber (32 Gene) und Viscera (38 Gene) von Atlantischem Kabeljau (Gadus morhua) isolierten Proben von P. bulbosa hochreguliert waren. Eine Erklärung für diesen subtilen Unterschied könnte ein Dauerstadium der P. bulbosa Larven zum Zeitpunkt der Probennahmen sein. Ob sich bestimmte Mikrohabitate innerhalb des Wirtes begünstigend auf den Parasiten auswirken, muss mit Hilfe experimenteller Studien gezeigt werden. Erste in Studie III erhobene Daten zum allergenen Potential von P. bulbosa sollten in serologischen Studien getestet werden. Als Grundlage für die Bewertung des pathogenen Potentials von P. bulbosa, sowie der weiteren Arten des P. decipiens Komplexes, sollten in experimentellen Studien NGS-Daten erhoben werden.
Im Rahmen dieser Dissertation wurde in drei methodisch unterschiedlichen Studien ein Bedarf besserer Referenzdaten aufgezeigt. Bestreben diese Datenlücken zu schließen, um das Potential der Methoden besser ausschöpfen zu können, müssen zukünftig noch weiter verstärkt werden.