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RNA interference (RNAi) has emerged as a powerful tool to induce loss-of-function phenotypes by post-transcriptional silencing of gene expression. In this study we wondered whether inducible RNAi-cassettes integrated into cellular DNA possess the power to trigger neoplastic growth. For this purpose inducible RNAi vectors containing tetracycline (Tet)-responsive derivatives of the H1 promoter for the conditional expression of short hairpin RNA (shRNA) were used to target human polo-like kinase 1 (Plk1), which is overexpressed in a broad spectrum of human tumors. In the absence of doxycycline (Dox) HeLa clones expressing TetR, that carry the RNAi-cassette stably integrated, exhibited no significant alteration in Plk1 expression levels. In contrast, exposure to Dox led to marked downregulation of Plk1 mRNA to 3% and Plk1 protein to 14% in cell culture compared to mismatch shRNA/Plk1-expressing cells. As a result of Plk1 depletion cell proliferation decreased to 17%. Furthermore, for harnessing RNAi for silencing disease-related genes in vivo we transplanted inducible RNAi-HeLa cells onto nude mice. After administration of Dox knockdown of Plk1 expression was observed correlating to a significant inhibition of tumor growth. Taken together, our data revealed that genomically integrated RNAi-elements are suitable to hamper tumor growth by conditional expression of shRNA.
Natural objects provide partially redundant information to the brain through different sensory modalities. For example, voices and faces both give information about the speech content, age, and gender of a person. Thanks to this redundancy, multimodal recognition is fast, robust, and automatic. In unimodal perception, however, only part of the information about an object is available. Here, we addressed whether, even under conditions of unimodal sensory input, crossmodal neural circuits that have been shaped by previous associative learning become activated and underpin a performance benefit. We measured brain activity with functional magnetic resonance imaging before, while, and after participants learned to associate either sensory redundant stimuli, i.e. voices and faces, or arbitrary multimodal combinations, i.e. voices and written names, ring tones, and cell phones or brand names of these cell phones. After learning, participants were better at recognizing unimodal auditory voices that had been paired with faces than those paired with written names, and association of voices with faces resulted in an increased functional coupling between voice and face areas. No such effects were observed for ring tones that had been paired with cell phones or names. These findings demonstrate that brief exposure to ecologically valid and sensory redundant stimulus pairs, such as voices and faces, induces specific multisensory associations. Consistent with predictive coding theories, associative representations become thereafter available for unimodal perception and facilitate object recognition. These data suggest that for natural objects effective predictive signals can be generated across sensory systems and proceed by optimization of functional connectivity between specialized cortical sensory modules.
Background The proto-oncogene pituitary tumor-transforming gene (PTTG) has been shown to be abundantly overexpressed in a large variety of neoplasms likely promoting neo-vascularization and tumor invasiveness. In this study, we investigated a potential role for PTTG mRNA expression as a marker to evaluate the future clinical outcome of patients diagnosed with primary cancer of the head and neck. Methods Tumor samples derived from primary tumors of 89 patients suffering from a squamous cell carcinoma were analyzed for PTTG mRNA-expression and compared to corresponding unaffected tissue. Expression levels were correlated to standard clinico-pathological parameters based on a five year observation period. Results In almost all 89 tumor samples PTTG was found to be overexpressed (median fold increase: 2.1) when compared to the unaffected tissue specimens derived from the same patient. The nodal stage correlated with PTTG transcript levels with significant differences between pN0 (median expression: 1.32) and pN+ (median expression: 2.12; P = 0.016). In patients who developed a tumor recurrence we detected a significantly higher PTTG expression in primary tumors (median expression: 2.63) when compared to patients who did not develop a tumor recurrence (median expression: 1.29; P = 0.009). Since the median expression of PTTG in patients with tumor stage T1/2N0M0 that received surgery alone without tumor recurrence was 0.94 versus 3.82 in patients suffering from a tumor recurrence (P = 0.006), PTTG expression might provide a feasible mean of predicting tumor recurrence. Conclusion Elevated PTTG transcript levels might be used as a prognostic biomarker for future clinical outcome (i.e. recurrence) in primary squamous cell carcinomas of the head and neck, especially in early stages of tumor development.
Wie wird man Formal- bzw. Sacherschließer?
Was macht ein Formal- bzw. Sacherschließer?
Unterschiedliche Traditionen. Ausblick in die Zukunft
Unterschiede in der derzeitigen Erschließungspraxis
Kooperative Erschließungsprogramme
Herkunft bibliografischer Datensätze: Wo kommen sie her?
Herausforderungen der Zukunft
The paper will provide a brief background to the history of the organization and cooperative efforts of African studies librarians in the United States including their efforts at international cooperation. Particular emphasis will be placed on the current opportunities for improved cooperation as digitization activities increase. Examples will include the DISA and Aluka initiatives and well as the Timbuktu manuscript digitization project at the Center for Research Libraries. Particular emphasis will placed on the possibilities for German-North American cooperation in the area of digital projects of historical photographs given the extensive collections held at Northwestern and Frankfurt.
... This year's Scientific Symposium of the University Library is already number six in the row. It was again prepared and organised like some of the previous conferences together with our North American partners. This means that a continuous specialists’ discussion and a professional partnership have been already installed. All librarians and information managers are invited to learn more about the results of this cooperation every year when it's time for the next Symposium during Frankfurt Book Fair. ...
Im Zuge der wachsenden Bedeutung von ELearning an Hochschulen beschäftigt sich diese Arbeit mit dem Einsatz von WebCT an der Johann Wolfgang Goethe Universität in Frankfurt am Main. In der vorliegenden Arbeit soll untersucht werden, inwieweit es nötig und möglich ist, WebCT hinsichtlich Benutzerfreundlichkeit, der Realisierung technischer Ideen und der pädagogischen Einsatzmöglichkeiten einzelner Tools zu optimieren. Aufgrund von Erfahrungen unsererseits, aus der Sicht als Kursteilnehmer mehrerer Lehrveranstaltungen mit Unterstützung von WebCT, im Sinne von Blended Learning und zu einem späteren Zeitpunkt als Tutoren in einem auf WebCT basierendem Seminars stellten wir fest, dass sich die Einbindung von WebCT als ELearning Plattform als aufwendig erwies. Dies könnte an mangelnden technischen Kenntnissen und Möglichkeiten liegen als auch an der nachlassenden Nutzung von Seiten der Studenten im Laufe des Semesters. Für uns stellte sich nun die Frage, wie WebCT optimiert werden kann, damit sich der didaktisch sinnvolle Einsatz in Lehrveranstaltungen erhöht. Die Diplomarbeit unterteilt sich in zwei Abschnitte; zum Ersten in die Analyse von WebCT, anhand der Kriterien und der Interviews und zum Zweiten in die Interviewauswertung und – interpretation einschließlich der technischen Optimierung.
Large American research libraries have been acquiring - by purchase and by lease - huge multi-disciplinary electronic collections of primary and secondary source materials. For example, the Digital Evans and Canadian Poetry easily make available to scholars primary materials that once were scattered in libraries across North America and Europe. The American State Papers, 1789 – 1838 collection allows easier searching of fragile rare materials. Collections made by libraries digitizing their own holdings, such the Archive of Early American Images from the John Carter Brown Library at Brown University, make research materials more discoverable and usable. Yet recent scholarship in American Studies by American and European scholars makes relatively little use of these new materials. Both disparities and congruities in what scholars use and what research libraries collect are apparent. Some simple reasons explain the dissonance. Furthermore, conversations with scholars suggest that materials and collections alone will not suffice to support research. Librarians’ skills and actions will increase the value of the new research materials.
We examine experimental signatures of TeV-mass black hole formation in heavy ion collisions at the LHC. We find that the black hole production results in a complete disappearance of all very high p_T (> 500 GeV) back-to-back correlated di-jets of total mass M > M_f ~ 1 TeV. We show that the subsequent Hawking-decay produces multiple hard mono-jets and discuss their detection. We study the possibility of cold black hole remnant (BHR) formation of mass ~ M_f and the experimental distinguishability of scenarios with BHRs and those with complete black hole decay. Finally we point out that a Heckler-Kapusta-Hawking plasma may form from the emitted mono-jets. In this context we present new simulation data of Mach shocks and of the evolution of initial conditions until the freeze-out.