Refine
Year of publication
- 2008 (2522) (remove)
Document Type
- Article (1000)
- Doctoral Thesis (315)
- Part of Periodical (273)
- Book (263)
- Working Paper (137)
- Part of a Book (134)
- Review (133)
- Report (91)
- Conference Proceeding (59)
- Preprint (27)
Language
- German (1593)
- English (768)
- French (47)
- Portuguese (43)
- Croatian (42)
- Italian (8)
- Multiple languages (8)
- Spanish (5)
- mis (4)
- dut (2)
Keywords
- Deutsch (48)
- Literatur (44)
- Rezension (43)
- Filmmusik (42)
- Germanistik (31)
- Russland (29)
- Linguistik (25)
- Übersetzung (23)
- Jelinek, Elfriede (20)
- Theater (18)
Institute
- Medizin (234)
- Extern (211)
- Präsidium (129)
- Biochemie und Chemie (87)
- Biowissenschaften (71)
- Physik (64)
- Erziehungswissenschaften (62)
- Gesellschaftswissenschaften (61)
- Center for Financial Studies (CFS) (59)
- Geowissenschaften (52)
In the present work, the photo-protection mechanisms in plants and purple bacteria were investigated experimentally at the molecular level. For this purpose, several spectroscopic methods were combined and applied to elucidate the function of carotenoids, pigments of the photosynthetic apparatus, in photo-protection. The experiments were focused on the mechanisms involved in quenching of singlet and triplet states of the electronically excited (bacterio)chlorophylls. This photosynthetic reaction events occur on an ultrafast time-scale. Measuring such short-lived events, and understanding the underlying principles, demand some of the most precise experiments and exact measurement technologies currently available. This implies certain requirements for the light source used: a suitable wavelength within the absorption band of the sample, sufficient power, and, most importantly, a pulse duration short compared to the studied reaction. Nowadays, we can achieve all this requirements using femtosecond-spectroscopic systems, which produce laser pulses shorter than 100 femtoseconds (fs). Transient absorption spectroscopy provides important information on molecular dynamics interrogating electronic transitions. The technique is based on photochemical generation of transient species with femtoseconds pump pulses and measuring transient absorption changes of the sample using a second, time delayed probe pulse which in this case is a spectrally broad white-light pulse.
Dass der Film eine enge Beziehung zum Mythos oder genauer: zu mythi-schen Erzählungen verschiedenster Art und Herkunft unterhält, ist be-kannt. Um sich klarzumachen, wie eng diese Beziehung im Einzelfall tat-sächlich sein kann, genügt es, an ein Projekt wie Star Wars zu denken, das sich sowohl offenkundig mythischer Muster als Modell für seine plots be-dient – so begegnet etwa der Kampf des Sohnes mit dem Vater schon in der griechischen und auch in der germanischen Mythologie –, als auch selbst einen neuen Mythos gestiftet hat. Oder man denkt an die in jüngster Zeit so beliebten Filme, die ganz explizit als Mythenverfilmun-gen angelegt sind wie Troy oder Beowulf. Oder aber man denkt an die zahllosen Beispiele für Filme, die die modernen Mythen der Popkultur wieder- und weitererzählen, wie zuletzt The Dark Knight und Quantum of Solace, oder, als mittlerweile klassisches Beispiel, Kill Bill. Insgesamt wird man sagen können, dass der Film einen nicht geringen Teil seiner Stoffe, Figuren und plots aus den verschiedenen Mythologien dieser Welt be-zieht, dass diese also gleichsam zu seinen wichtigsten Energiequellen ge-hören, und zwar in einem wahrscheinlich höheren Maße, als dies bei der Literatur des 20. Jahrhunderts der Fall ist.
Auf der Bundesgartenschau 2009 in Schwerin präsentieren sich alle deutschen UNESCO-Biosphärenreservate mit einer bisher einmaligen Ausstellung. Auf ca. 600 qm sind die 13 Regionen aus ganz Deutschland dabei, die mit dem internationalen Prädikat der UNESCO für eine modellhafte Entwicklung im ländlichen Raum stehen. Die BUGA-Besucher können durch eine Vielfalt an Exponaten, Medien und persönlicher Betreuung faszinierende Geschichten und Projekte aus diesen Nationalen Naturlandschaften von Weltrang erfahren.
Rezension zu Stefan Neuhaus: Märchen. Tübingen, Basel (A. Francke) 2005. 391 S.
Obwohl die zeittypischem Literaturverständnis geschuldete Zuordnung von André Jolles längst revidiert und das Märchen von der Forschung als sozusagen komplexe Form rehabilitiert wurde, fehlen neuere Überblicksdarstellungen zu der gerade für die Allgemeine und Vergleichende Literaturwissenschaft höchst interessanten Gattung. Diese Lücke zu schließen ist ambitiöses Ziel des vorliegenden Bandes.
Jene Veränderungen, denen die deutsche Universität durch den Bologna-Prozeß seit einigen Jahren unterworfen ist und die in der Einführung der Bachelor- und Master-Studiengänge ihren sinnfälligsten Höhepunkt finden, bescheren insbesondere den Geisteswissenschaften ein in Form und Umfang bislang nicht gekanntes Phänomen: die auf Studierende ausgerichtete Einführungs- und Studienliteratur.
Glucokinase (GK) is a key enzyme of glucose metabolism in liver and pancreatic beta-cells, and small molecule activators of GK (GKAs) are under evaluation for the treatment of type 2 diabetes. In liver, GK activity is controlled by the GK regulatory protein (GKRP), which forms an inhibitory complex with the enzyme. Here, we performed isothermal titration calorimetry and surface plasmon resonance experiments to characterize GK-GKRP binding and to study the influence that physiological and pharmacological effectors of GK have on the protein-protein interaction. In the presence of fructose-6-phosphate, GK-GKRP complex formation displayed a strong entropic driving force opposed by a large positive enthalpy; a negative change in heat capacity was observed (Kd = 45 nm, DeltaH = 15.6 kcal/mol, TDeltaS = 25.7 kcal/mol, DeltaCp = -354 cal mol(-1) K(-1)). With k(off) = 1.3 x 10(-2) s(-1), the complex dissociated quickly. The thermodynamic profile suggested a largely hydrophobic interaction. In addition, effects of pH and buffer demonstrated the coupled uptake of one proton and indicated an ionic contribution to binding. Glucose decreased the binding affinity between GK and GKRP. This decrease was potentiated by an ATP analogue. Prototypical GKAs of the amino-heteroaryl-amide type bound to GK in a glucose-dependent manner and impaired the association of GK with GKRP. This mechanism might contribute to the antidiabetic effects of GKAs.
The animated statues, robots and monsters in German Romantic narratives, as I will argue throughout, tell us something about the Romantic conception of the mutually embedded relationship between art and life. In the works of the German Romantics, the theme of artificial humans thus has an essentially autopoetic, or selfreflexive, function (cf. Schmitz-Emans 1993, 168f). It corresponds in exemplary fashion with Friedrich Schlegel´s idea of transcendental poetry, which should always be “poetry and simultaneously the poetry of poetry” (Schlegel [1985], 50). In the theme of artificial life as well as in transcendental poetry, the observation of the world is integrally bound up with the observation of art and the self (cf. Kremer [1996], 8ff).
Background Cyclin B1, the regulatory subunit of cyclin-dependent kinase 1 (Cdk1), is essential for the transition from G2 phase to mitosis. Cyclin B1 is very often found to be overexpressed in primary breast and cervical cancer cells as well as in cancer cell lines. Its expression is correlated with the malignancy of gynecological cancers. Methods In order to explore cyclin B1 as a potential target for gynecological cancer therapy, we studied the effect of small interfering RNA (siRNA) on different gynecological cancer cell lines by monitoring their proliferation rate, cell cycle profile, protein expression and activity, apoptosis induction and colony formation. Tumor formation in vivo was examined using mouse xenograft models. Results Downregulation of cyclin B1 inhibited proliferation of several breast and cervical cancer cell lines including MCF-7, BT-474, SK-BR-3, MDA-MB-231 and HeLa. After combining cyclin B1 siRNA with taxol, we observed an increased apoptotic rate accompanied by an enhanced antiproliferative effect in breast cancer cells. Furthermore, control HeLa cells were progressively growing, whereas the tumor growth of HeLa cells pre-treated with cyclin B1 siRNA was strongly inhibited in nude mice, indicating that cyclin B1 is indispensable for tumor growth in vivo. Conclusion Our data support the notion of cyclin B1 being essential for survival and proliferation of gynecological cancer cells. Concordantly, knockdown of cyclin B1 inhibits proliferation in vitro as well as in vivo. Moreover, targeting cyclin B1 sensitizes breast cancer cells to taxol, suggesting that specific cyclin B1 targeting is an attractive strategy for the combination with conventionally used agents in gynecological cancer therapy.