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This paper addresses remarks made by Flemming (2003) to the effect that his analysis of the interaction between retroflexion and vowel backness is superior to that of Hamann (2003b). While Hamann maintained that retroflex articulations are always back, Flemming adduces phonological as well as phonetic evidence to prove that retroflex consonants can be non-back and even front (i.e. palatalised). The present paper, however, shows that the phonetic evidence fails under closer scrutiny. A closer consideration of the phonological evidence shows, by making a principled distinction between articulatory and perceptual drives, that a reanalysis of Flemming’s data in terms of unviolated retroflex backness is not only possible but also simpler with respect to the number of language-specific stipulations.
The present study shows that though retroflex segments can be considered articulatorily marked, there are perceptual reasons why languages introduce this class into their phoneme inventory. This observation is illustrated with the diachronic developments of retroflexes in Norwegian (North- Germanic), Nyawaygi (Australian) and Minto-Nenana (Athapaskan). The developments in these three languages are modelled in a perceptually oriented phonological theory, since traditional articulatorily-based features cannot deal with such processes.
Woher kommt das neuerwachte Interesse an Sprachrichtigkeit? Woher kommt die ausgeprägte sprachliche Unsicherheit, die auch bei vielen hochgebildeten Menschen den Wunsch entstehen lässt, von Sprachpflegern über ihr Ureigenstes, nämlich ihre Muttersprache, belehrt zu werden? Obwohl Antworten auf diese Fragen letztlich spekulativ bleiben, wage ich doch die These, dass eine Ursache hierfür die Rechtschreibreform ist, die von einem Großteil der Bevölkerung nach wie vor nicht angenommen wird, die insgesamt weder zur Vereinfachung noch zu einer höheren Einheitlichkeit geführt hat; die aber andererseits ein öffentliches Nachdenken und Diskutieren über Sprachrichtigkeit in Gang setzte. – Jedenfalls ist die Verunsicherung ein Faktum, das von Linguisten nicht ignoriert werden sollte.
Ausgangspunkt: Die Kritik am "Zwei-Welten-Modell": Die grundlegende linguistische Unterscheidung zwischen "Sprache" und "Sprechen" ist im Rahmen der neueren Debatten um Sprachmedialität wieder verstärkt thematisiert und kritisiert worden. Lässt sich dieses schulbildende, in der Linguistik geradezu eherne Begriffspaar überhaupt noch sinnvollerweise aufrechterhalten? Oder muss es mindestens umdefiniert, vielleicht sogar gänzlich verworfen werden? Hat sich insbesondere die auf Chomsky zurückgehende Unterscheidung von Sprachkompetenz und -performanz nicht von selbst ad absurdum geführt, nachdem der linguistische Kognitivismus chomskyscher Provenienz Sprache als lebendiges Phänomen, als Medium menschlicher Kommunikation, vollständig aus dem Blick verloren hat? Führt nicht schon die scheinbar harmlose linguistische Differenzierung zwischen einer Sprachregel und ihrer Anwendung zu einer irreführenden und unangemessenen Verdinglichung von Sprache? ...
Poster presentation: NO-sensitive guanylyl cyclases (sGCs) are cytosolic receptors for nitric oxide (NO) catalyzing the conversion of GTP to cGMP. sGCs are obligate heterodimers composed of one alpha and beta subunit each. The allosteric mechanism of sGC activation via NO is well understood, however, our knowledge about alternative mechanisms such as protein-protein interactions regulating activity, availability, translocation and expression of sGC is rather limited. In a search by the yeast two-hybrid system using the catalytic domain of the alpha1 subunit as the bait, we have identified two structurally related proteins AGAP1 [1] and MRIP2 as novel sGC interacting proteins. MRIP2 is a multi-domain protein of 75 kDa comprising a single PH and ArfGAP domain each and two ankyrin repeats. Co-immunoprecipitation experiments using COS1 cells overexpressing both proteins demonstrated the interaction of MRIP2 with both subunits of the sGC alpha1beta1. Confocal microscopical analysis showed a prominent plasma membrane staining of MRIP2. This membrane association is mediated through an N-terminal myristoylation site and through binding of its PH domain to phospholipids such as phosphatidylinositol-3,5-bisphosphate (PI(3,5)P2). We hypothesize that MRIP2 may represent an acceptor protein for sGC that mediates recruitment of cytosolic sGC to the plasma membrane or other subcellular compartments.
Poster presentation: NO-sensitive guanylyl cyclases (GC) are the principal receptors for nitric oxide (NO) and convert GTP into the second messenger cGMP. We showed that GC is prone to tyrosine phosphorylation in COS1 cells overexpressing the human holoenzyme. Similar results were obtained in PC12 cells and in rat aortic tissue slices. The major phosphorylation site was mapped to position 192 in the regulatory domain of the beta1 subunit. Tyrosine phosphorylation of GC was reduced in the presence of the inhibitors PP1 and PP2 indicating that Src-like kinases are critically involved in phosphorylation. Moreover, co-immunoprecipitation experiments revealed an interaction between Src and GC. To further analyse the relevance of this posttranslational modification we generated a phospho-specific antibody raised against pTyr192. This antibody clearly distinguishes between phosphorylated and non-phosphorylated GC and may be a powerful tool to analyse the subcellular localisation of the phosphorylated enzyme.
Poster presentation NO-sensitive guanylyl cyclases (soluble guanylyl cyclase, sGC) are among the key regulators of intracellular cGMP concentration. The mechanisms underlying NO-mediated activation of sGC are quite well understood, however, little is known about the fine-tuning of sGC activity through alternative mechanisms such as protein phosphorylation. Several reports have demonstrated the reversible phosphorylation of sGC on serine/threonine residues, and it has been speculated, though not experimentally proven, that sGC might also be phosphorylated on tyrosine residues. Using broad-spectrum phosphatase inhibitors we were able to demonstrate tyrosine phosphorylation at Tyr192 of the beta 1 subunit of human sGC in COS1 cells. This residue forms part of a sequence segment (YEDL) representing a preferential binding site for SH2 domains of Src-like kinases. Pull-down assays and co-immunoprecipitation experiments showed that Src can indeed bind via its SH2 domain to pTyr192 of beta 1 indicating that tyrosine phosphorylation of sGC may be followed by recruitment of Src-like kinases to the phosphorylated beta 1 subunit. In support of this hypothesis, immunofluorescence studies showed a colocalization of overexpressed sGC and Src at the plasma membrane of COS1 and Hela cells. Together, our results point to an unexpected crosstalk between tyrosine kinase pathway(s) and the NO/cGMP signalling cascade which may result in translocation of the predominantly cytosolic sGC to the cytosolic face of the plasma membrane.