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Tanzania has been independent in 2011 for 50 years. While most neighbouring states have gone through violent conflicts, Tanzania has managed to implement extensive reforms without armed political conflicts, Hence, Tanzania is an interesting case for Peace and Development research. This dissertation analyses the political development in Tanzania since the introduction of the multiparty system in 1992, with a focus on the challenges for the democratisation process in connection with the 2000 and 2005 elections. The question of to what extent Tanzania had moved towards a consolidation of democracy, is analysed by looking at nine different institutions of importance for democratisation grouped in four spheres: the state, the political, civil and economic society. Focus is on the development of the political society, and the role of the opposition in particular. The analysis is based on secondary and primary material collected between September 2000 to April 2010. The main conclusion is that even if the institutions of liberal democracy have gradually developed, in practice single-party rule has continued, manifested in the 2005 election when the CCM won 92% of seats. Despite impressive economic growth, poverty remains deep and has not been substantially reduced. On a theoretical level this brings the old debate between liberal and substantive democracy back to the fore. Neither the economic nor the political reforms have brought about a transformation of the political and economic system resulting in the poor majority gaining substantially more political influence and improved economic conditions. Hence, it is argued that the interface between the economic, political and administrative reforms has not been sufficiently considered in the liberal democratic tradition. Liberal democracy is necessary for a democratic development, but not sufficient for democracy to be consolidated. For that a substantive democratic development is necessary.
Conviviality in Bellville: An Ethnography of Space, Place, Mobility and Being in Urban South Africa
(2014)
This book provides insight into the experiences of mobility and migration in contemporary South Africa, contributing to a field of literature about multiculturalism and urban public space in globalizing cities. It takes into consideration the greater international political and local socio-economic factors that drive migration, relationships and conviviality, and how they are intertwined in the everyday narrative of 'insiders' and 'outsiders'. The Bellville central business district demonstrates the realities of interconnected local and global hierarchies of citizenship and belonging and how they emerge in a world of accelerated mobility. The book further demonstrates how the emergence of conviviality in everyday public life represents a critical field for contemplating contemporary notions of human rights, citizenship and belonging.
As part of its on-going public dialogue program on progress in Ethiopia's development and public policy the Forum for Social Studies is undertaking a project of research and public dialogue on a number of selected topics on the theme of 'Prospects and Challenges for Inclusive and Participatory Development in Ethiopia'. The aim is to enable researchers and professionals to present evidence-based papers to stimulate debate and reflection. This first book in the program looks at the impact of development or lack of it, on specific social groups, namely women, young people and vulnerable groups that should be entitled to decent social care.
Elections provide a tremendous opportunity for national transformation and the pursuit of democratic practice. They can be a moment of national renewal. However, in most of Africa elections are often characterized by violent conflict as politicians seek to capture or maintain power through ethnic mobilization, propaganda and misrepresentation. Considering opportunities offered by information technology especially mobile phones and the discovery of extensive natural resources, Africa has an opportunity to significantly change the lives of ordinary citizens. But this transformation requires that youth are fully 'present' in the political, economic, social and cultural arenas. They will need to marshal their energies and stay focused on the things that are important for the continent of Africa. In the case of Kenya, youth should not wait to be invited to take up political leadership. Instead, they will need to invite themselves to the table and take advantage of the opportunity provided in Constitution and demand accountability and transparency in the conduct of national affairs. This book is part of ongoing work at Twaweza Communications in the pursuit of democracy, peace and justice. Themes covered include youth and leadership; elections and peace; youth as peace makers; family and global values among other topics.
From an exploration of the cooperative movement's various international iterations to a perspicacious survey of the history of cooperatives in Tanzania, Dr. Lyimo highlights the issues facing farmers and business people and illustrates the way in which cooperative effort- enterprises that put people, and not capital, at the center of their business- can not only improve members' economic power in bargaining for better marketing conditions and prices, but also to increase employment opportunities, thereby improving the standard of living for a large number of people.
It is the aim of this book while clarifying doubts and misconceptions, to provide a thorough reappraisal of the intellectual and rich cultural heritage of Islam with regards to the principles and practice of medicine and its representation to the world in the language of today. In nine chapters a range of topics are discussed including: The Promotion of Medical Education and Health Services; Personal and Environmental Hygiene; Circumcision; Manners of Eating; Social and Mental Heath; Curative Medicine; The Provision of Adequate and Potable Water; Magic, Witchcraft, Enchantments and Charms; Euthanasia; Suicide; The Rehabilitation of the Sick and the Needy; The Source of Human Creation; Sex Differentiation and Determination; Healing through Miracles; Magic and Soothsaying; HIV Infection and AIDS; Abortion; Females in Medical Practice; and The Challenges of Modem Medicine to Muslims.
Migration in the Service of African Development : Essays in honour of Professor Aderanti Adepoju
(2011)
Fifteen chapters are included here in this compendium in honour of the Nigerian migration scholar Professor Aderanti Adepoju. Though the authors come from diverse disciplinary backgrounds: geography, demography, sociology and law they all work within the fields of internal and international migration in Africa. Chapters on Uganda, Kenya, Botswana, Nigeria and Mali are devoted to aspects of internal migration, while those on African emigration to Mexico and migration between Burkina Faso and Côte d'Ivoire address various aspects of international migration. Migration issues in relation to women, students and climate change are also discussed.
The Millennium Development Goals address poverty, hunger, disease, illiteracy, environmental degradation, and discrimination against women, by the year 2015. In this volume scholars and policymakers in the fields of population and health reflect on the attainments of some of these goals, on the basis of empirical evidence in the Ghanaian context. The eight paper, with an introduction by the editors, synthesises papers presented at a seminar held in Ghana on ?Population, Health and Development in Relation to the Millennium Development Goals?, organised by the Population Association of Ghana.
Critical Issues in Nigerian Property Law, a collection of writings in honour of Professor Jelili Adebisi Omotola, SAN, a former Vice Chancellor of the University of Lagos, who died on the 29th of March 2006, has ten chapters that closely examine not only the current state of Property Law in Nigeria, but also recent developments and other challenges that have surfaced since the infamous Land Use Act of 1999. The book is clearly a useful contribution to a growing body of knowledge on property law and practice in Nigeria.
In 2009, Anglophone Cameroon literature celebrated its fifty years of existence. Now at the mature age of fifty plus this literature has a great deal to write home about even if it still has a lot to do in its pursuit of excellence. Part of its maturity resides in the fact that although the scale of literary creativity and literary criticism is skewed in favour of the former, Anglophone Cameroon literary criticism is gradually waking up from slumber in an attempt to catch up with the rapidly expanding creativity. The essays in this book comment practically on some aspects of all the genres of written literature that the Anglophone Cameroon creative writers have produced so far: the novel, drama, poetry, the short story, the essay and children's literature. The essays, on the whole, are a testimony of the transition and reality from the apparent drought of Anglophone Cameroon literary paucity to the actual fruitful period of Anglophone Cameroon abundance of literary creativity. The Anglophone Cameroonians have appropriated an imperial language, English, to serve their postcolonial Cameroonian vision. Their various literary texts are vehicles of representations that are essentially cultural and ideological constructs. The works examined are initially anchored on Cameroonian experiences to take on social significance. As they are grounded on moving human experiences, these works necessarily make references to the immediate Cameroonian environment of their authors before taking on universal human significance. The book abundantly evidences and crowns Shadrach Ambanasom's achievements and reputation as a skilled pedagogue on the art of practical literary criticism.
African land rights systems
(2014)
This book, from ethical, interdisciplinary, and African perspectives, unveils the root causes of the increasing land disputes. Its significance lies upon the effort of presenting a broad overview founded upon a critical analysis of the existing land-related disputes. It is a perspective that attempts to evaluate the renewed interest in evolving theories of land rights by raising questions that can help us to understand better differences underlying land ownership systems, conflict between customary and statutory land rights systems, and the politics of land reform. Other dimensions explored in the book include the market influence on land-grabbing and challenges accompanying trends of migration, resettlement, and integration. The methodology applied in the study provides a perspective that raises questions intended to identify areas of contention, dispute, and conflict. The study, which could also be categorized as a critical assessment of the African land rights systems, is intended to be a resource for scholars, activists, and organizations working to resolve land-related disputes.
Prevalent poverty and related problems in the East African region call for substantial action from various stakeholders, including social workers. This book, based on comprehensive empirical research, portrays an emerging yet powerful profession that has a significant role to play in the endeavour towards social development, social justice, human rights and gender equality. The book is the first of its kind to provide first-hand theoretical and empirical evidence about social work in East Africa.
The most popularised concept in the economics of innovation literature has been the national system of innovation (NSI). It was in the late 1980s that the concept that Frederik List coined as the National Political Economy of Production took off again with different thinkers writing about the peculiarities and distinctions of the Japanese, American, British, German, East Asian Tigers and other varieties of system construction. Freeman defines National System of Innovation as the network of institutions in the public and private sectors whose activities and interactions initiate, import, modify and diff use new technologies. Richard Nelson defines it as a set of institutions whose interactions determine the innovative performance of national firms. Lundvall defines the system of innovation as the elements and relationships which interact in the production, diffusion and use of new and economically useful knowledge and are either located within or rooted inside the borders of a nation state. The normative assumption is that those nations that succeeded in building economic strength relied on the science, engineering, technology and innovation capability that made them to achieve an innovation advantage to put them ahead in the world, acquiring national or regional economic leadership as the case may be depending on what level of analyses is selected to look at particular failure, success or progress they made.. In this volume we have a glimpse of how in different African economies from Ghana, Uganda, Kenya, South Africa and Nigeria specific cases have been taken to explore how systems of innovation is evolving.
The "Suma de tratos y contratos" (1569-1571) by Tomás de Mercado is the first legal treatise on trade that explicitly takes into account the specificities of Spanish trade with the Indias. Tomás de Mercado was faced with very profound changes in trade: long distances, large convoy sizes, the need for large amounts of funding, high risk, variations in prices and the value of money...
From a theological-legal point of view, these upheavals posed new and complex questions.
Mercado, advisor to the merchants of Seville and an excellent knowledge of New Spain, analyses the sudden transformation of economic and juridical practice with finesse and realism. The 'Suma' is thus an extraordinary real-time testimony to the profound transformations taking place in 16th century commerce.
Moreover, faced with fundamental questions of moral order and juridical legitimacy, Mercado proposes legal solutions of high equilibrium in which theological imperatives are masterfully reconciled with the needs of transatlantic commercial practice.
The blood-brain barrier (BBB) protects the brain microenvironment from external damage. It is formed by endothelial cells (ECs) lining the brain vessels, expressing tight junctions and having reduced transcytosis, resulting in a very low paracellular and transcellular passage of substances, respectively (low permeability). The specific BBB phenotype is maintained by Wnt molecules secreted by astrocytes (ACs) that bind to receptors in ECs, and start a molecular cascade that leads to β-catenin translocating to the nucleus and activating the transcription of BBB genes.
An increasing number of studies report BBB dysfunction in Alzheimer’s disease (AD), although the topic is currently under debate. AD is a neurodegenerative condition characterized by brain depositions of Aβ aggregates and Tau neurofibrillary tangles. The aetiology of AD is unknown, although round 5% of all AD cases have a genetic origin. Mutations in APP or PSEN1/2 can lead to Aβ over-production and accumulation, causing familiar AD. There is no cure for AD, as all clinical trials failed during the past years. Consequently, I studied the role of the BBB in AD, aiming to investigate if a BBB dysfunction occurs in AD, and to identify by transcriptomic analysis novel gene regulations happening at the BBB in AD. The final objective was to evaluate the potential of identified BBB genes as therapeutical target.
I used transgenic mice expressing the human APP mutations Swiss, Dutch and Iowa under the control of the neuronal promoter Thy1 (Thy1-APPSwDI) as AD model. In this AD mouse model, I could detect Aβ deposits and memory loss by immunofluorescence (IF) and behavioural tests. Importantly, I identified an increase of BBB permeability to 3-4 kDa dextrans in 6 months, 9-12 months, and 18 months or older AD mice compared to age-matched control wild types (WT), indicating BBB dysfunction in AD mice.
In order to study the BBB transcriptional changes in AD, I sequenced the RNA from 6 and 18 months old AD and WT mouse brain microvessels (MBMVs), as well as of FACS-sorted ECs, mural cells (MuCs), ACs, and microglia (MG) in collaboration with GenXPro, a company specialized in 3’ RNA sequencing. Currently, no transcriptomic datasets of ECs and MuCs are publicly available, suggesting that this is the first study sequencing those cell types in the context of AD.
The analysis of sequencing data from MBMVs and ECs revealed a Wnt/β-catenin repression, and an increase of inflammatory genes like Ccl3 in ECs, that could explain the BBB dysfunction observed in AD mice. Furthermore, the sequencing data from MuCs identified a set of 11 genes strongly regulated in both 6 and 18 month AD groups. Three of those 11 genes are known to be involved in inflammatory processes, demonstrating that inflammation affects and plays an important role in MuCs and ECs during AD.
Thanks to published sequencing data, some up-regulated MG genes in AD are well known and recognized, such as Trem2 and Apoe. Those genes were found in the FACS-sorted MG data as well, validating the AD model and with it, the other novel sequenced datasets. Importantly, one of the most strongly AD-regulated genes in MBMV and MG samples was Dkk2, a member of the Dickkopf family of secreted proteins known to be involved in Wnt signalling modulation. Importantly, a dual luciferase reporter assay proved that Dkk2 is a Wnt inhibitor. A preliminary immunohistochemistry examination of DKK2 in human brain autopsy tissue from an AD patient and age-matched control revealed a stronger DKK2 immunoreactivity in the AD brain.
In order to answer the question whether a rescue of BBB function would ameliorate AD symptoms, I made use of a tamoxifen-inducible transgenic mouse line to activate the Wnt/β-catenin pathway specifically in ECs, leading to a gain of function (GOF) condition (Cdh5-CreERT2+/–/Ctnnb1(Ex3)fl/fl). This mouse line was then crossed with the AD line, creating AD/GOF and AD/control groups.
AD/GOF mice performed better in a Y-Maze memory test than AD/controls when the Wnt/β-catenin pathway was induced before AD onset, indicating a protective effect. Moreover, the finding implies that shielding BBB functioning in AD further protects the brain from AD toxic effects, suggesting an important role of brain vasculature in AD and its potential as therapeutic target.
Synaptic plasticity is the activity dependent alteration of the composition, form and strength of synapses and believed to be the underlying mechanism of learning and memory formation. While initial changes in synaptic transmission are caused by second messenger signaling pathways and rapid modifications in the cytoskeleton, to achieve stable and persistent changes at individual synapses, the expression of new mRNAs and proteins is required. The central dogma postulated that the cell body is the only source of newly synthesized proteins. For neurons, with their unique morphology, this meant that proteins would need be transported long distances, often hundreds of microns, to reach their destined locations in dendrites and at spines. To overcome this limitation, neurons have developed a strategy to regulate protein synthesis locally by distributing thousands of mRNAs into neuronal processes and use them for local protein synthesis. Ample research has demonstrated the importance of local protein synthesis to many forms of long-term synaptic plasticity. One potential regulator of mRNA localization and local translation in neurons are non-coding RNAs. Intensive work over the past decades has highlighted the importance of non-coding RNAs in many aspects of brain function. The aim of this thesis is to obtain a better understanding of the role of non-coding RNAs in synaptic function and plasticity in the murine hippocampus. For this, we focused our studies on two classes of non-coding RNAs.
In the first part of my thesis, I describe our efforts on characterizing circular RNAs, a novel and peculiar family of non-coding RNAs, in the murine hippocampus by combining high throughput RNA-Sequencing with fluorescence in situ hybridization. Furthermore, we investigated the mechanisms of circular RNA biogenesis in hippocampal neurons by temporarily inhibiting spliceosome activity and analyzing the differentially regulated circular RNAs.
Die Forschung beschrieben in dieser Dissertation ist ein Teil der "European Research and Innovation Programme - PEARRL", und wurde von Horizon 2020 Marie Sklodowska-Curie actions der Europäischen Union, unter Förderungsnummer 674909 unterstützt.
In den letzten Jahren, wurde Senkung der Intensität der pharmazeutischen Forschung und Entwicklung beobachtet, da die Weiterentwicklung der Wirkstoffmolekülen hinzu einer "handlichen" Formulierung viele Schwierigkeiten aufweist. Meiste der neuen Wirkstoffkandidaten, die sich in Entwicklung befinden, haben suboptimale Eigenschaften in Bezug zur Löslichkeit und Auflösung und zeigen schlechte Bioverfügbarkeit, wenn eingenommen. Deswegen verlangen meiste neue Wirkstoffkandidate einen besonderen Ansatz in Bezug auf Formulierung, um akzeptable orale Bioverfügbarkeit zu erreichen. Diese neue Formulierungen werden meistens als “bio-enabling” Formulierungen bezeichnet und werden in Heilmittelentwicklung immer häufiger verwendet.
Hauptziel dieser Dissertation ist zu erforschen ob, durch Verbinden von biorelevanten in vitro Werkzeugen mit in silico Modeling und Simulationen, die in vitro Löslichkeit und Auflösung von bio-enabling Formulierungen mechanistisch erkläert und besser verstanden werden kann und somit eine erfolgreiche Simulation von in vivo Leistung erreicht werden kann.
Als Erstes wurden die physiologische Parameter, die die pharmakokinetik oraler Formulierungen beeinflüssen, identifiziert, indem die Auswirkung der Wirkstoffe, die zur Behandlung Magen-Darm-Krankheiten genutzt werden, sowie deren Pharmakokinetik, beurteilt wurde. Unter anderem wurde pH als einer der entscheinenden phyisiologischen Parameter erkannt, da es die Pharmakokinetik peroral verabreichter Stoffe signifikant beeinflüssen kann.
Als zweiter Schritt, mit besonderer Beachtung auf die Verwendung der biorelevanten in vitro Werkzeugen für die Erforschung der in vivo Auflösungsprozesse von bio-enabling Formulierungen, Fokus auf die biorelevante Medien und in vitro Apparaturen, die mögliche Prezipitationskinetik einschätzen können, wurde gesetzt. Biorelevante Medien sind wässrige Flüssigkeiten, die die Zusammensetzung der gastrointestinaler Flüssigkeiten nachmachen und für die Auflösungsuntersuchungen genutzt werden. Bis heute beinhalten die Aspirationsstudien die wichtigsten Hinweise und Informationen für den Design biorelevanter Medien. Es wurde beobachtet, dass die berichteten Werte mancher phyisiologisher Parameter erhebliche Unterschiede zwischen den Aspirationsstudien zeigen. Deswegen wurde untersucht, ob die Ergebnisse durch die Auswahl an Methodologie, die für die Entnahme und die Auswertung der Proben genutzt worden sind, beeinflusst werden können, wobei besondere Aufmerksamkeit den pH und der Pufferkapazität geschenkt wurde. Es wurde gezeigt, dass Unterschiede im Prozess der Probenhandhabung, z.B. Zentrifugieren und Lagerung einen deutlichen Einfluss auf die gemessenen Werte haben kann. Ausserdem, wurden in dieser Arbeit die in vitro Setups, die bisher in der Literatur zur Beurteilung der Übersättigung u.o. Ausfällung von Arzneimitteln im oberen Magen-Darm-Trakt vorgeschlagen wurden, überprüft und ihre Nützlichkeit und aktuelle Anwendung bewertet.
Nach Behebung der oben genannten Probleme, wurden zwei Fallbeispielformulierungen ausgewählt, um die Haupthypothese zu untersuchen. Die erste Formulierung ist auf den Markt unter dem Namen EMEND® und enthält den Wirkstoff in Nanoform. Die zweite Formulierung wird als INTELENCE® vermarktet und ist eine amorphe feste Dispersion des Wirkstoffs Etravirin. Durch die Wahl zwei unterschiedlicher Formulierungsansätzen konnten unterschiedliche Fallszenarien untersucht werden, wodurch umfassendere Vorschläge für die Bewältigung der Herausforderungen bei in vitro Experimenten und in silico Modelling mit bio-enabling Formulierungen möglich waren.
Bezogen auf den in dieser Dissertation beschriebenen Ansatz, ein mechanistisches Verständnis des in vivo Absorptionsprozesses, sowie eine erfolgreiche Simulation der nach der Verabreichung resultierenden Plasmaprofile einer bio-enabling-Formulierung der Nanoskala- und einer amorphen festen Dispersion wurde erreicht. Darüber hinaus wurden mögliche Wege vorgeschlagen, um einige Herausforderungen im Hinblick auf die Entwicklung von PBPK-Modellen für biofähige Formulierungen anzugehen. Diese Arbeit zeigt die mögliche Anwendung und Bedeutung der Absorptionsmodellierung für die rationale Formulierungsentwicklung und für die Stärkung des Wissens über Bio-Heilmittel in Bezug auf bio-enabling Formulierungen. Mithilfe dieses Ansatzes können die wesentlichen Parameter identifiziert werden, die das pharmakokinetische Verhalten schwerlöslicher Wirkstoffe beeinflussen, die als bio-enabling Formulierungen formuliert sind, und ermöglichen wiederum eine robuste Vorhersage der klinischen Ergebnisse.
Fokus meiner Doktorarbeit ist die Anwendung und Entwicklung NMR-spektroskopischer Methoden zur Charakterisierung zeitabhängiger Strukturänderungen von Biomolekülen – von lokalen dynamischen Veränderungen bis zur vollständigen Rückfaltung von Proteinen – und fasst die Ergebnisse meiner drei wichtigsten PhD-Projekte zusammen.
In meinem ersten Projekt habe ich die Leistung eines Temperatursprung-Probenkopfs – mit dem Proben mit hoher Salzkonzentration schnell erwärmt werden können – mithilfe einer Hochfrequenzspule technisch optimiert. Die optimierten Radiofrequenz-Bestrahlungsparameter, Lösungsmittel-bedingungen und der reduzierte Arbeitszyklus führten zu einem Temperatursprung von 20 °C in 400 ms. Ich habe eine Cystein-freie Mutante von Barstar hergestellt, die nach Zugabe von Harnstoff bei 0 °C kalt denaturiert werden kann, während sie ihren gefalteten Zustand bei 30 °C hält. Dadurch wurde auch ermöglicht, dass der Rückfaltungsprozess hunderte Male ohne Abbau oder Aggregation wiederholt werden kann. Die Kombination von reversibler Rückfaltung und rascher Temperaturänderung des kalt denaturierten Barstars ermöglichte die Entwicklung eines neuen kinetischen Experiments, bei dem der Rückfaltungsprozess von Barstar mit einem zweidimensionalen Echtzeit-NMR in hoher Zeitauflösung untersucht wird. Die vollständige Rückgratresonanzzuweisung wurde sowohl für den gefalteten als auch für den kalt denaturierten Zustand von Barstar durchgeführt und ergab, dass in der denaturierten Form beide Prolin-Reste einen gemischten Konformationszustand aufweisen. Dabei befindet sich die Tyr47-Pro48-Amidbindung im ungefalteten Zustand hauptsächlich in trans-, während im gefalteten Zustand in der seltenen cis-Konformation. Das neue hochauflösende kinetische Experiment zeigte, dass die Rückfaltung von Barstar durch die trans-cis-Isomerisierung der Tyr47-Pro48-Amidbindung verlangsamt wird, was sowohl die Sekundärstruktur als auch die Bildung der Tertiärstruktur beeinflusst. Basierend auf diesen Ergebnissen konnte ich einen plausiblen Faltungsmechanismus für den langsamen Faltungsweg von kalt denaturiertem Barstar skizzieren. Durch Änderung der Zeitparameter des Heizungszyklus wurde erreicht, dass die Tyr47-Pro48-Amidbindung im ungefalteten Zustand in der cis-Konformation bleibt und daher der schnelle Faltungsweg dominant wird. Das Starten des Magnetisierungstransfers vor der Temperaturänderung ermöglichte die Aufzeichnung eines Spektrums, das den entfalteten Zustand mit dem gefalteten Zustand korreliert. Dieses Spektrum ermöglichte quantitative Analysen des schnellen Faltungsweges und lieferte sogar indirekte Hinweise auf einen Zwischenzustand. Diese Methode aus Kombination von schnellem Temperatursprung und Kaltdenaturierung zeigt ein hohes Potenzial, Proteinfaltung auf atomarer Ebene experimentell zu untersuchen und ein tieferes Verständnis verschiedener Faltungswege zu erlangen.
In meinem zweiten Projekt – das Teil einer interdisziplinären Forschung war – konzentrierte ich mich auf die NMR-spektroskopische Charakterisierung von Nukleinsäuren, die mit einer photolabilen Schutzgruppe modifiziert wurden. Zuerst wurde mithilfe homonuklearer Korrelationsexperimente eine vollständige Protonresonanzzuweisung erreicht. Danach wurde die relative Konfiguration der photolabilen Schutzgruppen bestimmt basierend auf einer dreidimensionalen Modellstruktur und spezifischer NOE-Korrelationen. Des Weiteren wurde ein Strukturmodell unter Verwendung von NOE-Einschränkungen berechnet. Dieses Strukturmodell zeigte eine eingeschränkte Rotation um die CN-Bindung zwischen dem Käfig und der Nukleobase. Das Modell zeigte auch, dass der Käfig in der Hauptrille positioniert ist und nicht in das Lösungsmittel herausklappt. Im Vergleich zu einem zuvor charakterisierten NPE-Käfig führte die erhöhte Größe zu einer weiteren Senkung des Schmelzpunkts, zeigte jedoch einen geringeren Schmelzpunktunterschied zwischen der S- und der R-Konfiguration des Käfigs, wobei die S-Konfiguration zu einer größeren Reduktion des Schmelzpunktes führt. Dieser Trend wurde weiter untersucht und durch ein Screening unterstützt. Durch selektive Wasserinversions-Rückgewinnungsexperimente konnte ich auch zeigen, dass der Käfig die lokale Stabilität nur bis zu einer Entfernung von zwei benachbarten Basenpaaren von der Modifikationsstelle verringert. Die NOE-Daten dienten auch als guter Bezugspunkt, um die Qualität molekulardynamischer Simulationen zu testen, mit denen zusätzliche Käfigdesigns untersucht wurden. Die Kombination aus Synthese, NMR-Spektroskopie und MD-Simulationen ermöglichte bis jetzt die detaillierteste Untersuchung des Effekts vom Einbau eines einzelnen Käfigs zur Destabilisierung der DNA-Sekundärstruktur. Dabei wurden Einschränkungen des möglichen Designs aufgedeckt, aber auch die Entwicklung einer neuen, effizienteren Struktur ermöglicht.
Mein drittes Projekt konzentrierte sich auf die Charakterisierung eines RNA-Modellsystems. NMR-spektroskopische Daten von kleinen RNA-Modellsystemen – wie NOE, skalare Kopplungen, kreuzkorrelierte Relaxationsraten und RDC – sind eine unschätzbare Referenz für MD-Simulationen, obwohl die Menge der verfügbaren Literaturdaten – bis jetzt – sehr begrenzt ist. ...
Congenital diaphragmatic hernia (CDH) is a major congenital malformation with high mortality. Outcome data on larger unselected patient groups in Germany are unavailable as there is no registry for CDH. Therefore, routine data from the largest German health insurance fund were analyzed for the years 2009–2013. Main outcome measures were incidence, survival and length of hospital stay. Follow-up was 12 months. 285 patients were included. The incidence of CDH was 2.73 per 10,000 live births. Overall mortality was 30.2%. A total of 72.1% of the fatalities occurred before surgery. Highest mortality (64%) was noted in patients who were admitted to specialized care later as the first day of life. Patients receiving surgical repair had a better prognosis (mortality: 10.8%). A total of 67 patients (23.5%) were treated with ECMO with a mortality of 41.8%. The median cumulative hospital stay among one-year survivors was 40 days and differed between ECMO- and non-ECMO-treated patients (91 vs. 32.5 days, p < 0.001). This is the largest German cohort study of CDH patients with a one-year follow-up. The ECMO subgroup showed a higher mortality. Another important finding is that delayed treatment in specialized care increases mortality. Prospective clinical registries are needed to elucidate the treatment outcomes in detail.
Locator® and ball attachments are well-established systems to attach overdentures to two inter-foraminal implants. This study aimed to evaluate differences between the two systems regarding prosthetic maintenance and patients' oral-health-related quality of life (OHRQoL). Dental records of patients with a mandibular implant-retained overdenture were retrospectively analyzed. Prosthetic maintenance measures involving the denture suprastructure and attachment matrix and patrix were analyzed. Furthermore, the Oral Health Impact Profile-G14 (OHIP-G14) was used to evaluate OHRQoL. Results were analyzed by means of Kaplan–Meier analysis and Student's t- and log-rank tests. The records of 122 patients were evaluated. Kaplan–Meier survival analysis revealed a significant difference between ball attachments (Group B; n patients = 47) and Locator® attachments (Group L; n patients = 75) regarding the occurrence of denture fractures (p < 0.001) and events affecting the matrix (p = 0.028) and patrix (p = 0.030). Group L had a significantly lower total OHIP-G14 score than Group B (p = 0.002). The most common maintenance events were matrix-related and denture relining for both attachment systems. Group B required more maintenance measures than Group L. Moreover, patients in Group L had better OHRQoL than patients in Group B.
In spite of its surging popularity with scholars and environment conservation and management aid experts, scientific environmental epistemology does not seem to be the answer to the forestry and environmental problems that Africa is facing. Due to the lasting impacts of colonialism and therefore Western scientism on Africa, at the core of the conservation dilemma lies the conflict between scientific conservation epistemologies and 'local'/'indigenous' conservation epistemologies with the latter being the locals' potential workable solution to the environmental problems haunting the continent. It is in view of these circumstances that this book was born. The book is a clarion call for the revival and reinstitution of indigenous conservation and management epistemologies, not as a challenge to Western scientific conservation epistemologies, but to complement efforts by Western science in easing the tapestry of environmental problems that haunt Africa and the rest of the world. This is a valuable book for environmental conservationists, land resource managers, political/social ecologists, environmentalists, environmental anthropologists, environmental field workers and technicians, and practitioners and students of conservation sciences.
A Fallen Citadel and Other Poems is a powerful collection of over forty prose poems. The poems cover an array of issues ranging from the crisis that ensued after the 2007-2008 elections in Kenya to other social issues: loss of identity, poverty, hopelessness, and AIDs. These poems are powerful, vivid, full of imagery, and delightful. Some begin tragically, but end with hope; they begin with an everyday event, but end with a philosophical question about the meaning of life; and others are not only disturbing, but also thought provoking. Abala's poetic maneuvers in this collection are bound to delight and fascinate any reader.
Married But Available
(2008)
Married But Available ventures into a theme about which people say as much as they withhold. It explores intersections between sex, money and power, challenging orthodoxies, revealing complexities and providing insights into the politics and economics of relationships. During six months of fieldwork in Mimboland, Lilly Loveless, a Muzungulander doctoral student in Social Geography, researches how sex shapes and is shaped by power and consumerism in Africa. The bulk of her research takes place on the outskirts of the University of Mimbo, an institution where nothing is what it seems. Through her astounding harvest of encounters, interviews, conversations and observations, the reader gets a captivating glimpse into the frailty and resilience of human beings and society. Lilly Loveless comes out of it all well and truly baptized. And so does the reader!
The Sunbird
(2012)
The travails of day-to-day existence notwithstanding, Scolastica is an ordinary woman in Tobeningo who has struggled to carve a fairly successful life for herself. She is not a novice when it comes to putting out fires, thanks to a bubbling teenage daughter, a sugar-daddy chairman of the local Native Authority and the sudden re-appearance of a former sweetheart. But when her son sets out to commemorate his father's passing, on a false alarm, it is her life skills and sense of purpose that are tested to the limit. As the sole privy to the true situation, she is in a race against time to avert a calamity from befalling her son and her people. In her path, besides the vicissitudes of daily living, are the high-handedness and ineptitude of local politics, as well as the straightjacket and mysticism of both religion and tradition. How will she fare?
This book draws on years of rich empirical research on radio drama production in Cameroon to offer a strikingly new perspective in Development Theatre discourse in Africa. Chronicling the history and evolution of Development Theatre practice in Anglophone Africa and arguing for literary forms that address the basic everyday realities of ordinary people in a medium they understand, the book revisits the crucial question of utilitarian literature in a continent that continues to brandish a begging bowl even as it celebrates fifty years of independence. Radio Theatre's inherent latitude to reach the masses in a manner and matter that they identify with makes of it an invaluable albeit often neglected sub-genre in the universe of Development Theatre. Reaching an enlarged audience through radio drama productions - plays that address the rustic, ascetic and practical realities of the people - is liberating. Through radio plays and their capacity to provide for an enormous degree of authenticity, ordinary people are able to enhance their self-esteem. Like main stream Development Theatre, Radio Drama sets out to address the concerns of all in an all-embracing approach that explores interactive learning characterized by continuous questioning of and adaptation to reality. It disparages the omniscience of the superstructure meant to be perceived as indispensable and all-knowing. As a medium of development communication with unique aesthetic qualities found in and not limited to sound and silence, Radio Drama creates events and condenses reality into dramatic constellations with a high sense of authenticity that invites its audience to participate in the creation process with a strong sense of direction in a story, a plot and a moral. This people-oriented culture re-animation process is the fertile ground for grassroots empowerment. It is the point of departure for feasible development initiatives that this book explores.
Roselyne M. Jua has taught English and American Literature and Creative Writing at the Universities of Yaounde (1986-1993) and Buea (1993-2012). At the University of Buea, she served as Dean of Faculty of Arts from 2010 to 2012. She is Director of Academic Affairs at the University Bamenda, North West Region, since August 2012. Dr Jua has published articles in peer-reviewed journals and edited the plays of Victor E. Musinga among which+are The Barn+and+The Tragedy of Mr. No-Balance. She is co-author with Bate Besong of+To the Budding Creative Writer: A Handbook.
First Harvests are expressions of impressions on a variety of local and universal phenomena and states of being. It is the first ever poetry collection by a few sons and daughters of Nkongho-Mbo, a subgroup of the Mbo ethnic group of Cameroon, on which very little has been published. The poems however transcend the geographical, cultural and emotional landscapes that have inspired them.
During the 1990s, as the Internet in general and e-mail in particular grew in popularity as a means of communication, a number of Cameroonians residing in various parts of the world established a vibrant and lively electronic forum for the discussion of various issues related to their native land. The forum, known as Camnet, demonstrated that Cameroonians living abroad could actively participate in the political, economic and social processes taking place at home. This ability to remain actively engaged in the development of one's nation through the Internet is what Endeley calls 'virtual activism.' Camnet thus distinguished itself as the first and most influential breeding ground for Cameroonian 'virtual activism.' Although Camnet appeared to be dominated by political discussions, it was a truly multi-dimensional forum. No topic was explicitly forbidden and on some occasions the participants conducted extensive debates on issues that had nothing to do with Cameroon or with politics. In this publication, however, the author has chosen to present only a representative sample of his own contributions from the late 1990s with a direct bearing on Cameroon's development. Some of the contributions are in French and in order to reflect the bilingual nature of the debates that took place on Camnet, these have not been translated into English. The informed reader will be struck by the issues which were being debated over 15 years ago as well as by the fact that some of the predictions the author made in the 1990s are a reality today.
This book brings together twenty think-pieces on contemporary Human Rights issues at the international, regional and national level by one of Africa's foremost scholars of International Human Rights and Constitutional Law, J. Oloka-Onyango. Ranging from the 'Arab Spring' to the Right to Education, the collection is both an in-depth analysis of discrete topics as well as a critical reflection on the state of human rights around the world today. Taking up issues such as the African reaction to the International Criminal Court (ICC), the question of truth and reconciliation before the outbreak of post-election violence in Kenya and the links between globalization and racism, the book is a tour de force of issues that are both unique as well as pertinent to human rights struggles around the world.
Finding a Way Home
(2015)
Home is a place in ourselves where we are happy with ourselves, where we find peace with ourselves, where we are satisfied, fulfilled... The important theme coursing through all the stories in the novel, Finding a way home, is that we have to make the journey to find our homes, we have to find the path, and start walking in that path.
This book, the first of its kind on Anglophone Cameroon, brings significant local context into the practice of law particularly at a juncture when civil practice has been radically altered by Cameroon's ongoing effort at harmonization of both the substantive and procedural laws applicable in the courts. The book covers a wide spectrum of topics including: the commencement of civil actions, jurisdiction, simplified recovery procedures and measures of execution, provisional execution and stay of execution. It provides a detailed analysis of the relevant rules of court applicable in both the high court and court of appeal. One of its major strengths lies in its use of recent cases to demonstrate the way Cameroonian judges have dealt with local procedural laws, as well as how the differences between Cameroonian indigenous rules of practice and those imported particularly from Nigeria and England are reconciled.
Zimbabwe: The Urgency of Now
(2015)
Zimbabwe: The Urgency of Now, is a follow-up creative non-fiction book to Zimbabwe: The Blame Game. It goes further than The Blame Game and focuses on Zimbabwe in the GNU entity, the 2013 elections, post elections and post GNU Zimbabwe, and Now. They are a myriad number of problems, issues, limitations that still unbundles Zimbabwe's push towards multiparty democracy, social justice, economic sanity and growth, and The Urgency of Now focuses on the solutions to these. It also tackles the land reform in South Africa, how this could be its biggest problem going forward. It goes further and tackles the larger Africa problem toward democracy, growth, stability and unity, and why the progress towards the United States of Africa has been moribund.
Secrets, Silences, and Betrayals is an invitation to readers to consider factoring in the often discarded or censored but useful information held by the dominated. The books principal claim is that the unsaid weighs in significantly on the scale of semantic construction as that which is said. Thus, it legitimates the impact of the absentee in broadening and clarifying knowledge and understanding in most disciplines. In other words, just as exogenous epistemologies have underlain and explicated the basis for understanding diverse encounters-social, political, historical, cultural, literary, etc.-Secrets, Silences, and Betrayals challenges, from a pluridisciplinary angle, such highly dominant approaches to investigating the origin, nature, ways of knowing, and limits of human knowledge. It thus yields to the deontological basis to critically reexamine our understanding of the world around us. It is in this regard that the present volume points towards the need for human history to become a cumulative record and re-recording of every human journey and endeavor in life; it brings together disparate voices illuminating topical issues that would be or have been legated to posterity as nonexistent, partial, or half-truths.
Chieftaincy in Africa has displayed remarkable dynamics and adaptability to new socio-economic and political developments, without becoming totally transformed in the process. Almost everywhere on the continent, chiefdoms and chiefs have become active agents in the quest for ethnic, cultural symbols as a way of maximising opportunities at the centre of bureaucratic and state power, and at the home village where control over land and labour often require both financial and symbolic capital. Chieftaincy remains central to ongoing efforts at developing democracy and accountability in line with the expectations of Africans as individual 'citizens' and also as 'subjects' of various cultural communities. This book uses Cameroon and Botswana as case studies, to argue that the rigidity and prescriptiveness of modernist partial theories have left a major gap in scholarship on chiefs and chieftaincy in Africa. It stresses that studies of domesticated agency in Africa are sorely needed to capture the creative ongoing processes and to avoid overemphasising structures and essentialist perceptions on chieftaincy and the cultural communities that claim and are claimed by it.
The African conundrum... is rooted out of the historical, philosophical and cultural bastardisation, imbalances and inequalities which many post-colonial African governments have always sought to address, though with varying degrees of success, since the 1960s. Lamentably, this African conundrum is rarely examined in a systematic manner that takes into account the geopolitical milieu of the continent, past and present. This volume seeks to interrogate and examine the extent of the impact of the geopolitical seesaw which seems poised to tip in favour of the Global North. The book grapples with the question on how Africa can wake up from its cavernous intellectual slumber to break away from both material and psychological dependency and achieve a transformative political and socio-economic self-reinvention and self-assertion. While the African conundrum is largely a result of historic oppression and a resilient colonial legacy, this book urges Africans to rethink their condition in a manner that makes Africa responsible and accountable for its own destiny. The book argues that it is through this rethinking that Africa can successfully transcend the logic of post-imperial dependency.