Refine
Year of publication
Document Type
- Article (83)
- Preprint (8)
- Conference Proceeding (3)
- Diploma Thesis (1)
- Working Paper (1)
Has Fulltext
- yes (96)
Is part of the Bibliography
- no (96)
Keywords
- SARS-CoV-2 (3)
- Accelerators & Beams (2)
- Atomic, Molecular & Optical (2)
- COVID-19 (2)
- NMR spectroscopy (2)
- Outcome (2)
- RNA (2)
- Stroke (2)
- hypoxia (2)
- 3years (1)
- ARDS (1)
- ASCT (1)
- Accelerators & storage rings (1)
- Anti-kaon–nucleon physics (1)
- Aortic valve (1)
- Aortic valve replacement (1)
- Atomic & molecular beams (1)
- Attention-Deficit/Hyperactivity Disorder (ADHD) (1)
- Autism spectrum disorder (1)
- B.1.1.7 (1)
- BI1361849 (1)
- Baryonic resonances (1)
- Beam loss (1)
- Blood flow restriction (1)
- Brain asymmetry (1)
- CHIP (1)
- COVID (1)
- CV9202 (1)
- CVID (1)
- Cardiac surgery (1)
- Cerebrovascular (1)
- Charge-transfer collisions (1)
- Circular accelerators (1)
- Circulating miRNA (1)
- Clinical Trials and Observations (1)
- Clinical competence (1)
- Clinical frailty scale (1)
- Clinical trial (1)
- Contingent Negative Variation, CNV (1)
- Continuous Performance Test, CPT (1)
- Coronary heart disease (1)
- Couch tracking (1)
- Covid19-nmr (1)
- Critical care (1)
- Crystal structure (1)
- Cue-P3 (1)
- DME (1)
- Devic disease (1)
- Devic syndrome (1)
- EEG (1)
- Echovirus-30 (1)
- Ejection fraction (1)
- Electron-pion identification (1)
- Electronic transitions (1)
- Endurance training (1)
- European Society for Immunodeficiencies (ESID) (1)
- Event related potentials (1)
- Experimental models of disease (1)
- FFPE (1)
- Fast-track (1)
- Fibre/foam sandwich radiator (1)
- Flexible Endoscopic Evaluation of Swallowing (1)
- Frailty (1)
- Freezeout (1)
- GRAND-SLAM (1)
- German PID-NET registry (1)
- Gimbaled tracking (1)
- HAI‐1 (1)
- HGF (1)
- HIBCPP cells (1)
- Heavy-ion reactions (1)
- Hemispheric specialization (1)
- Heterogeneity (1)
- High-pressure (1)
- Hyperons (1)
- Hypofractionated radiotherapy (1)
- ICU (1)
- ILUVIEN (1)
- IgG substitution therapy (1)
- Immunomonitoring (1)
- Intensive care outcome (1)
- Ionisation energy loss (1)
- Kaonic nuclei (1)
- Language delay (1)
- Low & intermediate-energy accelerators (1)
- Low energy QCD (1)
- MLC tracking (1)
- Molecular biology (1)
- Multi-wire proportional drift chamber (1)
- Multianvil (1)
- Muscular diseases (1)
- Myeloid Neoplasia (1)
- N501Y (1)
- NADPH oxidase (1)
- NMO-IgG (1)
- NMR (1)
- Nervous system diseases (1)
- Neural network (1)
- Neurofeedback (1)
- Neuromyelitis optica (1)
- Non-small cell lung cancer (1)
- Normative modeling (1)
- Nox1 (1)
- NoxO1 (1)
- Nuclear astrophysics (1)
- Nuclear physics of explosive environments (1)
- Nuclear reactions (1)
- Nucleus (1)
- Organ motion (1)
- PID prevalence (1)
- Partial wave analysis (1)
- Photon counting (1)
- Polarization (1)
- Prognostic models (1)
- Proton (1)
- RNA stability (1)
- RNA, long noncoding (1)
- Radiation detectors (1)
- Radiative capture (1)
- Randomized Controlled Trial (1)
- Rare-earth borate (1)
- Reactive oxygen species (1)
- Readmission (1)
- Response control (1)
- Retro-IDEAL (1)
- Robotic tracking (1)
- SLAM-seq (1)
- SWATH (1)
- Slow Cortical Potentials, SCP (1)
- Stenosis (1)
- Steroid (1)
- Strangeness (1)
- Superoxide (1)
- Sustained attention (1)
- TR (1)
- Telomere length (1)
- Telomeres (1)
- Terbium (1)
- Thrombosis (1)
- Tracking (1)
- Transient (1)
- Transient ischemic attack (1)
- Transition radiation detector (1)
- Trigger (1)
- VIGALL (1)
- VIP1 (1)
- Vertigo (1)
- Water balance (1)
- White blood cells (1)
- Xenon-based gas mixture (1)
- accessory proteins (1)
- accident (1)
- adaptation (1)
- antibiotic therapy (1)
- aquaporin-4 (AQP4) antibody (1)
- arteriogenesis (1)
- autologous stem cell transplantation (1)
- available water capacity (1)
- biomarker (1)
- blood flow restriction (1)
- blood-cerebrospinal fluid barrier (1)
- bone healing (1)
- bone marrow mononuclear cells (1)
- bortezomib (1)
- breast tumor (1)
- cell-free protein synthesis (1)
- cerebrospinal fluid (1)
- chemotherapy (1)
- circulating miRNA (1)
- clinical features (1)
- clonal dominance (1)
- clonal hematopoiesis (1)
- dE/dx (1)
- de novo transcription (1)
- depression (1)
- drought-stress (1)
- drug resistance (1)
- enterovirus (1)
- epidemiology (1)
- epigenomics (1)
- evolution (1)
- fecal microbiota transfer (1)
- forest site classification (1)
- fragment screening (1)
- genetic diversity (1)
- glioblastoma (1)
- graft-vs-host disease (1)
- hematopoietic stem cells (1)
- hematopoietic stress (1)
- hepatitis C virus (HCV) (1)
- heteronuclear detection (1)
- human allogeneic hematopoietic cell transplantation (1)
- human decellularized dermis (1)
- human intestinal microbiota (1)
- hypertension, pulmonary (1)
- imaging (1)
- induced membrane (1)
- induction regimen (1)
- infections: pneumonia, TB, viral (1)
- injury (1)
- intrinsically disordered region (1)
- isotope labeling (1)
- kidney (1)
- large functional RNAs (1)
- lenalidomide (1)
- leukemia (1)
- lipid raft (1)
- lockdown (1)
- longitudinally extensive transverse myelitis (1)
- mRNA active cancer immunotherapy (1)
- macrophage (1)
- magnetic resonance imaging (1)
- miR (1)
- miR-142-5p (1)
- miR-143-3p (1)
- miR-197-3p (1)
- miR-342-3p (1)
- miR-424-5p (1)
- microdosing (1)
- mitochondrial antiviral signaling protein (MAVS) (1)
- molecular adaptation (1)
- molecular biology (1)
- molecular dynamics (1)
- molecular surveillance (1)
- monocytes (1)
- multiple myeloma (1)
- natural selection (1)
- neovascularization, physiologic (1)
- nonstructural proteins (1)
- obesity (1)
- p47phox (1)
- pediatric intensive care (1)
- peripheral artery disease (1)
- ppK − (1)
- pressure cycling technology (1)
- primary immunodeficiency (PID) (1)
- proliferation (1)
- protease inhibitor (1)
- proteome (1)
- recurrent optic neuritis (1)
- registry for primary immunodeficiency (1)
- renal failure (1)
- replicative fitness (1)
- resistance mutation (1)
- serine protease (NS3-4A) (1)
- site classification system (1)
- sleepiness (1)
- somatic mutations (1)
- spike mutation (1)
- strength training (1)
- structural proteins (1)
- structure constraints (1)
- trauma (1)
- tumor (1)
- tumor microenvironment (1)
- tumor‐associated macrophages (1)
- viral infection (1)
- x-ray techniques (1)
Institute
- Medizin (53)
- Physik (26)
- Buchmann Institut für Molekulare Lebenswissenschaften (BMLS) (5)
- ELEMENTS (5)
- Geowissenschaften (5)
- Biochemie und Chemie (4)
- Frankfurt Institute for Advanced Studies (FIAS) (4)
- Informatik (4)
- Sonderforschungsbereiche / Forschungskollegs (3)
- Biochemie, Chemie und Pharmazie (2)
Purpose: Physical activity is associated with altered levels of circulating microRNAs (ci-miRNAs). Changes in miRNA expression have great potential to modulate biological pathways of skeletal muscle hypertrophy and metabolism. This study was designed to determine whether the profile of ci-miRNAs is altered after different approaches of endurance exercise. Methods: Eighteen healthy volunteers (aged 24 ± 3 years) participated this three-arm, randomized-balanced crossover study. Each arm was a single bout of treadmill-based acute endurance exercise at (1) 100% of the individual anaerobic threshold (IANS), (2) at 80% of the IANS and (3) at 80% of the IANS with blood flow restriction (BFR). Load-associated outcomes (fatigue, feeling, heart rate, and exhaustion) as well as acute effects (circulating miRNA patterns and lactate) were determined. Results: All training interventions increased the lactate concentration (LC) and heart rate (HR) (p < 0.001). The high-intensity intervention (HI) resulted in a higher LC than both lower intensity protocols (p < 0.001). The low-intensity blood flow restriction (LI-BFR) protocol led to a higher HR and higher LC than the low-intensity (LI) protocol without BFR (p = 0.037 and p = 0.003). The level of miR-142-5p and miR-197-3p were up-regulated in both interventions without BFR (p < 0.05). After LI exercise, the expression of miR-342-3p was up-regulated (p = 0.038). In LI-BFR, the level of miR-342-3p and miR-424-5p was confirmed to be up-regulated (p < 0.05). Three miRNAs and LC show a significant negative correlation (miR-99a-5p, p = 0.011, r = − 0.343/miR-199a-3p, p = 0.045, r = − 0.274/miR-125b-5p, p = 0.026, r = − 0.302). Two partial correlations (intervention partialized) showed a systematic impact of the type of exercise (LI-BFR vs. HI) (miR-99a-59: r = − 0.280/miR-199a-3p: r = − 0.293). Conclusion: MiRNA expression patterns differ according to type of activity. We concluded that not only the intensity of the exercise (LC) is decisive for the release of circulating miRNAs—as essential is the type of training and the oxygen supply.
Neurogenic dysphagia is one of the most frequent and prognostically relevant neurological deficits in a variety of disorders, such as stroke, parkinsonism and advanced neuromuscular diseases. Flexible endoscopic evaluation of swallowing (FEES) is now probably the most frequently used tool for objective dysphagia assessment in Germany. It allows evaluation of the efficacy and safety of swallowing, determination of appropriate feeding strategies and assessment of the efficacy of different swallowing manoeuvres. The literature furthermore indicates that FEES is a safe and well-tolerated procedure. In spite of the huge demand for qualified dysphagia diagnostics in neurology, a systematic FEES education has not yet been established. The structured training curriculum presented in this article aims to close this gap and intends to enforce a robust and qualified FEES service. As management of neurogenic dysphagia is not confined to neurologists, this educational programme is applicable to other clinicians and speech–language therapists with expertise in dysphagia as well. The systematic education in carrying out FEES across a variety of different professions proposed by this curriculum will help to spread this instrumental approach and to improve dysphagia management.
Following votes in the Coniacian Working Group, the Cretaceous Subcommission and the International Commission on Stratigraphy, on May 1st, 2021, the International Union of Geological Sciences voted unanimously to ratify the Global Stratotype Section and Point (GSSP) proposal for the base of the Coniacian Stage of the Upper Cretaceous Series and Cretaceous System. The lower boundary of the Coniacian Stage is placed at the base of Bed 46 of the Salzgitter-Salder section in northern Germany. The boundary is defined by the first appearance of the inoceramid bivalve species Cremnoceramus deformis erectus (Meek) and complemented by the Navigation carbon isotope event. Additional data include the bivalve genus Didymotis, foraminifera, ammonite, nannofossil and organic-walled dinoflagellate cyst events. Three auxiliary sections (Słupia Nadbrzeżna, central Poland; Střeleč, Czech Republic; El Rosario, NE Mexico) supplement the details of the boundary record in various facies, and in differing geographic and biogeographic contexts.
Mutations of the isocitrate dehydrogenase-1 (IDH1) and IDH2 genes are among the most frequent alterations in acute myeloid leukemia (AML) and can be found in ∼20% of patients at diagnosis. Among 4930 patients (median age, 56 years; interquartile range, 45-66) with newly diagnosed, intensively treated AML, we identified IDH1 mutations in 423 (8.6%) and IDH2 mutations in 575 (11.7%). Overall, there were no differences in response rates or survival for patients with mutations in IDH1 or IDH2 compared with patients without mutated IDH1/2. However, distinct clinical and comutational phenotypes of the most common subtypes of IDH1/2 mutations could be associated with differences in outcome. IDH1-R132C was associated with increased age, lower white blood cell (WBC) count, less frequent comutation of NPM1 and FLT3 internal tandem mutation (ITD) as well as with lower rate of complete remission and a trend toward reduced overall survival (OS) compared with other IDH1 mutation variants and wild-type (WT) IDH1/2. In our analysis, IDH2-R172K was associated with significantly lower WBC count, more karyotype abnormalities, and less frequent comutations of NPM1 and/or FLT3-ITD. Among patients within the European LeukemiaNet 2017 intermediate- and adverse-risk groups, relapse-free survival and OS were significantly better for those with IDH2-R172K compared with WT IDH, providing evidence that AML with IDH2-R172K could be a distinct entity with a specific comutation pattern and favorable outcome. In summary, the presented data from a large cohort of patients with IDH1/2 mutated AML indicate novel and clinically relevant findings for the most common IDH mutation subtypes.
BACKGROUND: Recent findings support the idea that interleukin (IL)-22 serum levels are related to disease severity in end-stage liver disease. Existing scoring systems--Model for End-Stage Liver Disease (MELD), Survival Outcomes Following Liver Transplantation (SOFT) and Pre-allocation-SOFT (P-SOFT)--are well-established in appraising survival rates with or without liver transplantation. We tested the hypothesis that IL-22 serum levels at transplantation date correlate with survival and potentially have value as a predictive factor for survival.
MATERIAL AND METHODS: MELD, SOFT, and P-SOFT scores were calculated to estimate post-transplantation survival. Serum levels of IL-22, IL-6, IL-10, C-reactive protein (CRP), and procalcitonin (PCT) were collected prior to transplantation in 41 patients. Outcomes were assessed at 3 months, 1 year, and 3 years after transplantation.
RESULTS: IL-22 significantly correlated with MELD, P-SOFT, and SOFT scores (Rs 0.35, 0.63, 0.56 respectively, p<0.05) and with the discrimination in post-transplantation survival. IL-6 showed a heterogeneous pattern (Rs 0.40, 0.63, 0.57, respectively, p<0.05); CRP and PCT did not correlate. We therefore added IL-22 serum values to existing scoring systems in a generalized linear model (GLM), resulting in a significantly improved outcome prediction in 58% of the cases for both the P-SOFT (p<0.01) and SOFT scores (p<0.001).
CONCLUSIONS: Further studies are needed to address the concept that IL-22 serum values at the time of transplantation provide valuable information about survival rates following orthotopic liver transplantation.
Clonal hematopoiesis of indeterminate potential (CHIP) is caused by recurrent somatic mutations leading to clonal blood cell expansion. However, direct evidence of the fitness of CHIP-mutated human hematopoietic stem cells (HSCs) in blood reconstitution is lacking. Because myeloablative treatment and transplantation enforce stress on HSCs, we followed 81 patients with solid tumors or lymphoid diseases undergoing autologous stem cell transplantation (ASCT) for the development of CHIP. We found a high incidence of CHIP (22%) after ASCT with a high mean variant allele frequency (VAF) of 10.7%. Most mutations were already present in the graft, albeit at lower VAFs, demonstrating a selective reconstitution advantage of mutated HSCs after ASCT. However, patients with CHIP mutations in DNA-damage response genes showed delayed neutrophil reconstitution. Thus, CHIP-mutated stem and progenitor cells largely gain on clone size upon ASCT-related blood reconstitution, leading to an increased future risk of CHIP-associated complications.
Background: Autism spectrum disorder (“autism”) is a highly heterogeneous neurodevelopmental condition with few effective treatments for core and associated features. To make progress we need to both identify and validate neural markers that help to parse heterogeneity to tailor therapies to specific neurobiological profiles. Atypical hemispheric lateralization is a stable feature across studies in autism, but its potential as a neural stratification marker has not been widely examined. Methods: In order to dissect heterogeneity in lateralization in autism, we used the large EU-AIMS (European Autism Interventions—A Multicentre Study for Developing New Medications) Longitudinal European Autism Project dataset comprising 352 individuals with autism and 233 neurotypical control subjects as well as a replication dataset from ABIDE (Autism Brain Imaging Data Exchange) (513 individuals with autism, 691 neurotypical subjects) using a promising approach that moves beyond mean group comparisons. We derived gray matter voxelwise laterality values for each subject and modeled individual deviations from the normative pattern of brain laterality across age using normative modeling. Results: Individuals with autism had highly individualized patterns of both extreme right- and leftward deviations, particularly in language, motor, and visuospatial regions, associated with symptom severity. Language delay explained most variance in extreme rightward patterns, whereas core autism symptom severity explained most variance in extreme leftward patterns. Follow-up analyses showed that a stepwise pattern emerged, with individuals with autism with language delay showing more pronounced rightward deviations than individuals with autism without language delay. Conclusions: Our analyses corroborate the need for novel (dimensional) approaches to delineate the heterogeneous neuroanatomy in autism and indicate that atypical lateralization may constitute a neurophenotype for clinically meaningful stratification in autism.
NMR spectroscopy is a potent method for the structural and biophysical characterization of RNAs. The application of NMR spectroscopy is restricted in RNA size and most often requires isotope‐labeled or even selectively labeled RNAs. Additionally, new NMR pulse sequences, such as the heteronuclear‐detected NMR experiments, are introduced. We herein provide detailed protocols for the preparation of isotope‐labeled RNA for NMR spectroscopy via in vitro transcription. This protocol covers all steps, from the preparation of DNA template to the transcription of milligram RNA quantities. Moreover, we present a protocol for a chemo‐enzymatic approach to introduce a single modified nucleotide at any position of any RNA. Regarding NMR methodology, we share protocols for the implementation of a suite of heteronuclear‐detected NMR experiments including 13C‐detected experiments for ribose assignment and amino groups, the CN‐spin filter heteronuclear single quantum coherence (HSQC) for imino groups and the 15N‐detected band‐selective excitation short transient transverse‐relaxation‐optimized spectroscopy (BEST‐TROSY) experiment.
Basic Protocol 1: Preparation of isotope‐labeled RNA samples with in vitro transcription using T7 RNAP, DEAE chromatography, and RP‐HPLC purification
Alternate Protocol 1: Purification of isotope‐labeled RNA from in vitro transcription with preparative PAGE
Alternate Protocol 2: Purification of isotope‐labeled RNA samples from in vitro transcription via centrifugal concentration
Support Protocol 1: Preparation of DNA template from plasmid
Support Protocol 2: Preparation of PCR DNA as template
Support Protocol 3: Preparation of T7 RNA Polymerase (T7 RNAP)
Support Protocol 4: Preparation of yeast inorganic pyrophosphatase (YIPP)
Basic Protocol 2: Preparation of site‐specific labeled RNAs using a chemo‐enzymatic synthesis
Support Protocol 5: Synthesis of modified nucleoside 3′,5′‐bisphosphates
Support Protocol 6: Preparation of T4 RNA Ligase 2
Support Protocol 7: Setup of NMR spectrometer for heteronuclear‐detected NMR experiments
Support Protocol 8: IPAP and DIPAP for homonuclear decoupling
Basic Protocol 3: 13C‐detected 3D (H)CC‐TOCSY, (H)CPC, and (H)CPC‐CCH‐TOCSY experiments for ribose assignment
Basic Protocol 4: 13C‐detected 2D CN‐spin filter HSQC experiment
Basic Protocol 5: 13C‐detected C(N)H‐HDQC experiment for the detection of amino groups
Support Protocol 9: 13C‐detected CN‐HSQC experiment for amino groups
Basic Protocol 6: 13C‐detected “amino”‐NOESY experiment
Basic Protocol 7: 15N‐detected BEST‐TROSY experiment
Climate change and its impacts already pose considerable challenges for societies that will further increase with global warming (IPCC, 2014a, b). Uncertainties of the climatic response to greenhouse gas emissions include the potential passing of large-scale tipping points (e.g. Lenton et al., 2008; Levermann et al., 2012; Schellnhuber, 2010) and changes in extreme meteorological events (Field et al., 2012) with complex impacts on societies (Hallegatte et al., 2013). Thus climate change mitigation is considered a necessary societal response for avoiding uncontrollable impacts (Conference of the Parties, 2010). On the other hand, large-scale climate change mitigation itself implies fundamental changes in, for example, the global energy system. The associated challenges come on top of others that derive from equally important ethical imperatives like the fulfilment of increasing food demand that may draw on the same resources. For example, ensuring food security for a growing population may require an expansion of cropland, thereby reducing natural carbon sinks or the area available for bio-energy production. So far, available studies addressing this problem have relied on individual impact models, ignoring uncertainty in crop model and biome model projections. Here, we propose a probabilistic decision framework that allows for an evaluation of agricultural management and mitigation options in a multi-impact-model setting. Based on simulations generated within the Inter-Sectoral Impact Model Intercomparison Project (ISI-MIP), we outline how cross-sectorally consistent multi-model impact simulations could be used to generate the information required for robust decision making.
Using an illustrative future land use pattern, we discuss the trade-off between potential gains in crop production and associated losses in natural carbon sinks in the new multiple crop- and biome-model setting. In addition, crop and water model simulations are combined to explore irrigation increases as one possible measure of agricultural intensification that could limit the expansion of cropland required in response to climate change and growing food demand. This example shows that current impact model uncertainties pose an important challenge to long-term mitigation planning and must not be ignored in long-term strategic decision making.
In order to achieve climate change mitigation, long-term decisions are required that must be reconciled with other societal goals that draw on the same resources. For example, ensuring food security for a growing population may require an expansion of crop land, thereby reducing natural carbon sinks or the area available for bio-energy production. Here, we show that current impact-model uncertainties pose an important challenge to long-term mitigation planning and propose a new risk-assessment and decision framework that accounts for competing interests.
Based on cross-sectorally consistent simulations generated within the Inter-Sectoral Impact Model Intercomparison Project (ISI-MIP) we discuss potential gains and limitations of additional irrigation and trade-offs of the expansion of agricultural land as two possible response measures to climate change and growing food demand. We describe an illustrative example in which the combination of both measures may close the supply demand gap while leading to a loss of approximately half of all natural carbon sinks.
We highlight current limitations of available simulations and additional steps required for a comprehensive risk assessment.