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Proteins encoded by small open reading frames (sORFs) have a widespread occurrence in diverse microorganisms and can be of high functional importance. However, due to annotation biases and their technically challenging direct detection, these small proteins have been overlooked for a long time and were only recently rediscovered. The currently rapidly growing number of such proteins requires efficient methods to investigate their structure–function relationship. Herein, a method is presented for fast determination of the conformational properties of small proteins. Their small size makes them perfectly amenable for solution-state NMR spectroscopy. NMR spectroscopy can provide detailed information about their conformational states (folded, partially folded, and unstructured). In the context of the priority program on small proteins funded by the German research foundation (SPP2002), 27 small proteins from 9 different bacterial and archaeal organisms have been investigated. It is found that most of these small proteins are unstructured or partially folded. Bioinformatics tools predict that some of these unstructured proteins can potentially fold upon complex formation. A protocol for fast NMR spectroscopy structure elucidation is described for the small proteins that adopt a persistently folded structure by implementation of new NMR technologies, including automated resonance assignment and nonuniform sampling in combination with targeted acquisition.
Vermutlich in den 1340er Jahren verfasste ein anonymer ostfränkischer Dichter, der sich selbst als "kuenig vom Otenwalde" oder schlicht "kuenig" bezeichnet, zwölf höchst originelle Reimpaargedichte, die gemeinsam im sogenannten 'Hausbuch des Michael de Leone', einer bedeutenden Würzburger Sammelhandschrift, überliefert sind. Viel Resonanz haben diese bemerkenswerten Gedichte wie auch ihr Verfasser in der Forschung allerdings nicht gefunden, lässt man die Versuche, den Texten realienkundlich verwertbare Aussagen zur mittelalterlichen Alltagskultur zu entlocken, einmal beiseite. Wohl versuchte man verschiedentlich, eine historische Autorpersönlichkeit hinter dem prätentiösen Pseudonym festzumachen, was zwar zu mehr oder weniger gewagten Spekulationen führte, letztlich aber keine befriedigenden Ergebnisse zeitigte. Abgesehen davon jedoch begnügte man sich anstelle einer eingehenden literaturwissenschaftlichen Analyse im Großen und Ganzen damit, den König vom Odenwald als Kuriosum am Rande, weitab vom literarischen Kanon, zu vermerken.