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By comparing two distinct governmental organizations (the US military and NASA) this paper unpacks two main issues. On the one hand, the paper examines the transcripts that are produced as part of work activities in these worksites and what the transcripts reveal about the organizations themselves. Additionally, the paper analyses what the transcripts disclose about the practices involved in their creation and use for practical purposes in these organizations. These organizations have been chosen as transcription forms a routine part of how they operate as worksites. Further, the everyday working environments in both organizations involve complex technological systems, as well as multi-party interactions in which speakers are frequently spatially and visually separated. In order to explicate these practices, the article draws on the transcription methods employed in ethnomethodology and conversation analysis research as a comparative resource. In these approaches audio-video data is transcribed in a fine-grained manner that captures temporal aspects of talk, as well as how speech is delivered. Using these approaches to transcription as an analytical device enables us to investigate when and why transcripts are produced by the US military and NASA in the specific ways that they are, as well as what exactly is being re-presented in the transcripts and thus what was treated as worth transcribing in the interactions they are intended to serve as documents of. By analysing these transcription practices it becomes clear that these organizations create huge amounts of audio-video “data” about their routine activities. One major difference between them is that the US military selectively transcribe this data (usually for the purposes of investigating incidents in which civilians might have been injured), whereas NASA’s “transcription machinery” aims to capture as much of their mission-related interactions as is organizationally possible (i.e., within the physical limits and capacities of their radio communications systems). As such the paper adds to our understanding of transcription practices and how this is related to the internal working, accounting and transparency practices within different kinds of organization. The article also examines how the original transcripts have been used by researchers (and others) outside of the organizations themselves for alternative purposes.
The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.