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On the distribution of Astrobunus laevipes CANESTRINI, 1872 (Arachnida: Opiliones) in Central Europe
(2001)
Published data and unpublished communications show that the range of Astrobunus laevipes in Central Europe is much larger than previously believed. The present review extends the list of records to the German states of Baden-Württemberg, Hessia, Rhineland Palatinate, Bavaria, Northrhine-Westfalia, Saxony, and Lower Saxony and provides a map of the present distribution in Germany. Furthermore, it lists new findings of A. laevipes in the Czech Republic and Hungary (Rakaca/Serehat Valley). Records of A. laevipes in Austria are not included in this review.
The usefulness of propylene glycol as capture preservative in pitfall traps, with the aim of using the captured spiders for DNA barcoding, was tested. For this purpose a laboratory experiment on the conserving and/or denaturing effect of propylene glycol on mitochondrial DNA (COI) was set up. For the experiment 110 specimens of the common and abundant wolf spider species Pardosa lugubris were manually captured, killed and incubated from one to four weeks in either pure or watered propylene glycol or 70 % denatured ethanol. Rates of successful sequencing, following a standard protocol, did not differ between samples incubated in propylene glycol and in the more commonly used ethanol. Thus, within four weeks, propylene glycol did not significantly denaturize mitochondrial DNA. In two field studies, pitfall traps with propylene glycol captured more spiders than traps with acetic acid. The effect was significant only in one of two field trials, but then consistent at three different sites and the three dominant spider families. Based on these results and our operating experience, we recommend propylene glycol as a capture preservative for (pitfall) traps to obtain specimens for DNA barcoding identification.