Refine
Year of publication
- 1976 (2)
Document Type
- Article (2)
Language
- English (2)
Has Fulltext
- yes (2)
Is part of the Bibliography
- no (2)
Keywords
Institute
Antiserum against crystallized 20β-hydroxysteroid dehydrogenase from Streptomyces hydrogenans was used for different immunodiffusion and immunoprecipitation tests to quantify the bacterial enzyme in cell-free supernatants of the microorganism. After immunoprecipitation and gel electrophoresis the molecular weight of the subunits of 20β-hydroxysteroid dehydrogenase was calculated to be 27 300 ± 700.
Levels of the purine nucleoside triphosphates are de creasing towards the end of log phase growth of Streptomyces hydrogenans. Induction of 20β-hydroxysteroid dehy-drogenase by addition of 11β,21-dihydroxy-4,17 (20) -pregna-dien-3-one to the growth medium leads to a pronounced drop in purine nucleoside triphosphate levels with is irreversible in contrast to the initial loss and later accumulation of RNA.