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The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
The current paper explores these two sorts of phonetic explanations of the relationship between syllabic position and the voicing contrast in American English. It has long been observed that the contrast between, for example, /p/ and /b/ is expressed differently, depending on the position of the stop with respect to the vowel. Preceding a vowel within a syllable, the contrast is largely one of aspiration. /p/ is aspirated, while /b/ is voiceless, or in some dialects voiced or even an implosive. Following a vowel within a syllable, both /p/ and /b/ both tend to lack voicing in the closure and the contrast is expressed largely by dynamic differences in the transition between the previous vowel and the stop. Here, vowel and closure duration are negatively correlated such that the /p/ has a shorter vowel and longer closure duration. This difference is often enhanced by the addition of glottalization to /p/. In addition to these differences, there are additional differences connected to higher-level organization involving stress and feet edges. To make the current discussion more tractable, we will restrict ourselves to the two conditions (CV and VC) laid out above.