Refine
Year of publication
Document Type
- Article (43)
- Conference Proceeding (1)
- Preprint (1)
Has Fulltext
- yes (45)
Is part of the Bibliography
- no (45)
Keywords
- Hodgkin lymphoma (5)
- classical Hodgkin lymphoma (3)
- B-cell lymphoma (2)
- DNA methylation (2)
- Histology (2)
- Lymphocytes (2)
- anaplastic large cell lymphoma (2)
- Antiviral therapy (1)
- B cell malignancies (1)
- B cells (1)
- B-cell (1)
- B-cell immunology (1)
- B-cell transcription factors (1)
- BATF3 (1)
- BEACOPP (1)
- Caco-2 cells (1)
- Cancer (1)
- Cancer detection and diagnosis (1)
- Cancer genomics (1)
- Carotid injury (1)
- Cell differentiation (1)
- Cell motility (1)
- Cell staining (1)
- Colorectal cancer (1)
- Cytoplasm (1)
- Cytoplasmic staining (1)
- Cytoskeletal proteins (1)
- Diffuse large B cell lymphoma (1)
- Endocrine cancer (1)
- FOXO3a (1)
- GLUT1 (1)
- Gene regulation (1)
- Glycolysis (1)
- Hashimoto’s thyroiditis (1)
- Hematoxylin staining (1)
- Hepatitis C (1)
- IgG4-related disease (1)
- Image analysis (1)
- Image processing (1)
- Immunohistochemistry techniques (1)
- Immunology (1)
- Indeterminate biliary stricture (1)
- Inflammation (1)
- Lymph nodes (1)
- Lymphoid tissues (1)
- Lymphoma (1)
- MTCL (1)
- Machine learning (1)
- Metastasis (1)
- Metastatic tumors (1)
- Molecular subtypes (1)
- NADPH oxidase (1)
- NF-κB (1)
- Neuroendocrine cancer (1)
- Next-generation sequencing (1)
- Nodular lymphocyte predominant Hodgkin lymphoma (1)
- Non-Hodgkin lymphoma (1)
- Nox4 (1)
- Oncology (1)
- PCR (1)
- Pathologists (1)
- Petri net (1)
- Reactive oxygen species (1)
- Restenosis (1)
- SW480 cells (1)
- Single-cell RNA sequencing (1)
- T cell/histiocyte rich large B cell lymphoma (1)
- T-cell homeostasis (1)
- T-cell niche (1)
- Transformation (1)
- Tumor heterogeneity (1)
- Warburg effect (1)
- agent-based modeling (1)
- artificial intelligence (1)
- autophagy (1)
- bile duct stenosis (1)
- cHL (1)
- cell motility (1)
- chemokine receptors (1)
- cholangiocarcinoma (1)
- computer vision (1)
- differentiated thyroid carcinoma (1)
- diffuse large B cell lymphoma (1)
- digital pathology (1)
- dissemination (1)
- endoscopic retrograde cholangiopancreatography (1)
- endoscopy (1)
- eosinophilic cholangitis (1)
- epigenetic (1)
- fibroblasts (1)
- gene expression (1)
- gene expression analysis (1)
- gene expression profiling (1)
- gene therapy (1)
- germinal center (1)
- histopathological growth pattern (1)
- host response (1)
- human lymph node (1)
- image analysis (1)
- immune response (1)
- immunohistochemistry (1)
- interim positron emissiontomography (1)
- loss of B-cell phenotype (1)
- luteolin (1)
- lymph node (1)
- lymphoma pathogenesis (1)
- mature T-cell lymphoma (1)
- methylation profiling (1)
- miRNA (1)
- microRNA (1)
- microdissection (1)
- microsatellite instability (1)
- mir-148a (1)
- modeling (1)
- morphological filtering (1)
- motility (1)
- nodular lymphocyte predominant Hodgkin lymphoma (1)
- nodular lymphocyte-predominant Hodgkin lymphoma (1)
- nodular sclerosis (1)
- oncogene (1)
- ordinary differential equation (1)
- partial differential equation (1)
- primary sclerosing cholangitis (1)
- rosetting T cells (1)
- segmentation (1)
- shock filter (1)
- sirtuin1 (1)
- targeted therapy (1)
- vitamin D (1)
- vitamin D receptor (1)
- whole slide image (1)
Institute
Background The differential diagnosis between follicular thyroid adenoma and minimal invasive follicular thyroid carcinoma is often difficult for several reasons. One major aspect is the lack of typical cytological criteria in well differentiated specimens. New marker molecules, shown by poly- or monoclonal antibodies proved helpful. Methods We performed global gene expression analysis of 12 follicular thyroid tumours (4 follicular adenomas, 4 minimal invasive follicular carcinomas and 4 widely invasive follicular carcinomas), followed by immunohistochemical staining of 149 cases. The specificity of the antibody was validated by western blot analysis Results In gene expression analysis QPRT was detected as differently expressed between follicular thyroid adenoma and follicular thyroid carcinoma. QPRT protein could be detected by immunohistochemistry in 65% of follicular thyroid carcinomas including minimal invasive variant and only 22% of follicular adenomas. Conclusion Consequently, QPRT is a potential new marker for the immunohistochemical screening of follicular thyroid nodules.
Meeting Abstract : Deutsche Gesellschaft für Chirurgie. 125. Kongress der Deutschen Gesellschaft für Chirurgie. Berlin, 22.-25.04.2008 Einleitung: Beim follikulären Schilddrüsenkarzinom ist die prae- und intraoperative Diagnose nur eingeschränkkt möglich. Immunhistochemische und immuncytologische Marker können hier hilfreich sein. Bei Patienten mit follikulären Karzinomen ist auf mRNA-Ebene eine up-Regulation für qprt (quinolinate-phosphoribosyltransferase) zu beobachten. Material und Methoden: Seit Januar 2004 werden bei Patienten mit prae- und intraoperativem Verdacht auf Schilddrüsenkarzinom, Frischgewebeproben zur Genexpressions-Analyse asserviert. Die so gewonnen Ergebnisse wurden an prospektiv und retrospektiv gewonnenen Präparaten von follikulären Adenomen und follikulären Karzinomen überprüft [Gruppe A]. Bei 20 prospektiven Punktionscytologien mit follikulärer Neoplasie werden aktuell zusätzliche immuncytologische Färbungen auf qprt durchgeführt und mit den nachfolgenden histologischen und immunhistochemischen Ergebnissen der Operationspräparate verglichen. Ergebnisse: Gruppe A (151 Patienten): bei 79 follikulären Adenomen und 72 follikulären Karzinomen wurden immunhistochemische Färbungen auf qprt durchgeführt. Hier zeigten sich 60 der 79 Adenome richtig negativ (76%) und 47 der 72 Karzinome richtig positiv (66%). Dies ergab eine Treffsicherheit zwischen Adenom und Karzinom von 71%. Gruppe B (20 Patienten): Die Ergebnisse der Korrelation zwischen präoperativer Immuncytochemie und postoperativer Diagnose und Immunhistochemie stehen derzeit noch aus. Schlussfolgerung: qprt ist ein immunhistochemischer Marker für follikuläre Schilddrüsenkarzinome mit einer befriedigenden Trennschärfe zwischen Adenom und Karzinom. Zusätzlich stellt qprt möglicherweise einen aussagekräftigen präoperativen immuncytochemischen Marker mit Auswirkung auf OP-Zeitpunkt, OP-Verfahren und OP-Taktik dar.
The human immune system is determined by the functionality of the human lymph node. With the use of high-throughput techniques in clinical diagnostics, a large number of data is currently collected. The new data on the spatiotemporal organization of cells offers new possibilities to build a mathematical model of the human lymph node - a virtual lymph node. The virtual lymph node can be applied to simulate drug responses and may be used in clinical diagnosis. Here, we review mathematical models of the human lymph node from the viewpoint of cellular processes. Starting with classical methods, such as systems of differential equations, we discuss the values of different levels of abstraction and methods in the range from artificial intelligence techniques formalism.
Burkitt lymphoma (BL) is the most common B-cell lymphoma in children. Within the International Cancer Genome Consortium (ICGC), we performed whole genome and transcriptome sequencing of 39 sporadic BL. Here, we unravel interaction of structural, mutational, and transcriptional changes, which contribute to MYC oncogene dysregulation together with the pathognomonic IG-MYC translocation. Moreover, by mapping IGH translocation breakpoints, we provide evidence that the precursor of at least a subset of BL is a B-cell poised to express IGHA. We describe the landscape of mutations, structural variants, and mutational processes, and identified a series of driver genes in the pathogenesis of BL, which can be targeted by various mechanisms, including IG-non MYC translocations, germline and somatic mutations, fusion transcripts, and alternative splicing.
Background: Protective effects of vitamin D have been reported in autoimmune and malignant thyroid diseases, though little is known about the underlying mechanism. Sirtuin 1 histon deacethylase (SIRT1) links the vitamin D pathway with regulation of transcription factor FOXO3a, a key player in cell cycle regulation and apoptosis. Aim of the present study was to investigate common single nucleotide polymorphisms (SNP's) in FOXO3a gene in respect to thyroid diseases, as well as to evaluate the hypothesis of Sirtuin1-FOXO3a interaction being a mediator of anti-proliferative vitamin D effects.
Methods: The SNP's FOXO3a rs4946936/rs4945816/rs9400239 were genotyped in 257 patients with differentiated thyroid carcinoma (DTC), 139 patients with Hashimoto thyroiditis (HT) and 463 healthy controls (HC). Moreover, T-helper cells of HC and papillary thyroid cancer cell line BCPAP were incubated with 1,25(OH)2D3 and/or SIRT1 inhibitor Ex-527 in order to elucidate SIRT1- dependent vitamin D effects on cell proliferation and FOXO3a gene expression in vitro.
Results: Patients with DTC tended to carry more often allele C in FOXO3a rs4946936 in comparison to HC (pcorrected = pc = 0.08). FOXO3a rs9400239T and rs4945816C was more frequent in HT in comparison to HC (pc = 0.02 and pc = 0.01, respectively). In both DTC and HT, we could not find a correlation of FOXO3a SNP's with vitamin D status. However, on in vitro level, 1,25(OH)2D3 showed an anti-proliferative effect in both T-helper cells and BCPAP, that was blocked by SIRT1 inhibition (T-helper cells: p = 0.0059, BCPAP: p = 0.04) and accompanied by elevated FOXO3a gene expression in T-helper cells (p = 0.05).
Conclusions: FOXO3a rs9400239T and rs4945816C may constitute risk factors for HT, independent of the vitamin D status.This indicates the implication of FOXO3a in pathogenesis of autoimmune thyroid diseases. The dependency of anti-proliferative vitamin D effects on SIRT1 activity further suggests a key role of vitamin D-SIRT1-FOXO3a axis for protective vitamin D effects.