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The seasonal changes of phytoplankton and selected ecological parameters (temperature, pH, oxygen, conductivity, nutrients) were studied in the years 1998 and 1999 at gravel-pit Chomoutov (Olomouc, the Czech Republic). Long-term changes in the species composition and proportions of the main algal groups during last three decades are discussed. Subsequent eutrophication was found with the come back of water blooms of Aphanizomenon flos-aquae.