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- 14C-Labeled Terpenoids (1)
- Geranylgeranyl Pyrophosphate (1)
- I50 Value (1)
- Kaurene (1)
- Phvtoene Desaturase (1)
- Phvtofluene (1)
- Phytoene (1)
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- WL110547 (1)
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- ζ-Carotene (1)
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A non-radioactive cell-free assay was developed to quantitatively determine inhibition of plant-type phytoene desaturase by bleaching herbicides. An active desaturase was prepared from an appropriately cloned E. coli transformant. Another E. coli transformant was used to produce the required phytoene. Phytofluene and t-carotene, the products of the desaturase reaction, were either determined by HPLC or optical absorption spectra. Enzyme kinetics and inhibition data for the bleaching tetrazole herbicide WL110547 are presented as an example.
Methods are described for an enzymatic preparation of 14C-labeled terpenoids. With a cell-free system of a white mutant of Phycomyces blakesleeanus (Mucoraceae) [14C]squalene and [14C- cis]phytoene can be synthesized from [2-14C]mevalonate. The application of norflurazon, a phenyl- pyridazinone herbicide, helps to increase the yield of squalene. Furthermore, the liquid endosperm of Echinocystis lobata (Cucurbitaceae) was used for the formation of either [14C(-)]kaurene from [14C]mevalonic acid or [14C-/ra/w]geranylgeranyl pyrophosphate in the presence of Amo 1618.
The hydrocarbons formed were purified by alumina-column chromatography and preparative thin-layer chromatography (TLC). Geranylgeranyl pyrophosphate was separated by DE-column chromatography followed by TLC.
Measurements of the branching fractions for D⁺ → Kₛ⁰Kₛ⁰K⁺, Kₛ⁰Kₛ⁰π⁺ and D⁰ → Kₛ⁰Kₛ⁰, Kₛ⁰Kₛ⁰Kₛ⁰
(2016)
By analyzing 2.93 fb−1 of data taken at the ψ(3770) resonance peak with the BESIII detector, we measure the branching fractions for the hadronic decays D+→K0SK0SK+, D+→K0SK0Sπ+, D0→K0SK0S and D0→K0SK0SK0S. They are determined to be B(D+→K0SK0SK+)=(2.54±0.05stat.±0.12sys.)×10−3, B(D+→K0SK0Sπ+)=(2.70±0.05stat.±0.12sys.)×10−3, B(D0→K0SK0S)=(1.67±0.11stat.±0.11sys.)×10−4 and B(D0→K0SK0SK0S)=(7.21±0.33stat.±0.44sys.)×10−4, where the second one is measured for the first time and the others are measured with significantly improved precision over the previous measurements.
Measurements of the branching fractions for D⁺ → Kₛ⁰Kₛ⁰K⁺, Kₛ⁰Kₛ⁰π⁺ and D⁰ → Kₛ⁰Kₛ⁰, Kₛ⁰Kₛ⁰Kₛ⁰
(2016)
By analyzing 2.93 fb−1 of data taken at the ψ(3770) resonance peak with the BESIII detector, we measure the branching fractions for the hadronic decays D+→K0SK0SK+, D+→K0SK0Sπ+, D0→K0SK0S and D0→K0SK0SK0S. They are determined to be B(D+→K0SK0SK+)=(2.54±0.05stat.±0.12sys.)×10−3, B(D+→K0SK0Sπ+)=(2.70±0.05stat.±0.12sys.)×10−3, B(D0→K0SK0S)=(1.67±0.11stat.±0.11sys.)×10−4 and B(D0→K0SK0SK0S)=(7.21±0.33stat.±0.44sys.)×10−4, where the second one is measured for the first time and the others are measured with significantly improved precision over the previous measurements.