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Author

  • Faltus, Timo (2)
  • Strebhardt, Klaus (2)
  • Kaufmann, Manfred (1)
  • Krämer, Andrea (1)
  • Loibl, Sibylle (1)
  • Yuan, Juping (1)
  • Zimmer, Brigitte (1)

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  • 2004 (1)
  • 2005 (1)

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  • Article (2)

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  • HER2/neu (1)
  • Krebs (1)
  • Protein-Kinasen (1)
  • RNA interference (1)
  • RNA-Interferenz (1)
  • Therapie (1)
  • Tumormarker (1)
  • antiproliferative activity (1)
  • apoptosis (1)
  • siRNA (1)
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  • Medizin (2)

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Silencing of the HER2/neu gene by siRNA inhibits proliferation and induces apoptosis in HER2/neu-overexpressing breast cancer cells (2004)
Faltus, Timo ; Yuan, Juping ; Zimmer, Brigitte ; Krämer, Andrea ; Loibl, Sibylle ; Kaufmann, Manfred ; Strebhardt, Klaus
In eukaryotes, double-stranded (ds) RNA induces sequence-specific inhibition of gene expression referred to as RNA interference (RNAi). We exploited RNAi to define the role of HER2/neu in the neoplastic proliferation of human breast cancer cells. We transfected SK-BR-3, BT-474, MCF-7, and MDA-MB-468 breast cancer cells with short interfering RNA (siRNA) targeted against human HER2/neu and analyzed the specific inhibition of HER2/neu expression by Northern and Western blots. Transfection with HER2/neu-specific siRNA resulted in a sequence-specific decrease in HER2/neu mRNA and protein levels. Moreover, transfection with HER2/neu siRNA caused cell cycle arrest at G0/G1 in the breast cancer cell lines SKBR-3 and BT-474, consistent with a powerful RNA silencing effect. siRNA treatment resulted in an antiproliferative and apoptotic response in cells overexpressing HER2/neu, but had no influence in cells with almost no expression of HER2/neu proteins like MDA-MB-468 cells. These data indicate that HER2/neu function is essential for the proliferation of HER2/neuoverexpressing breast cancer cells. Our observations suggest that siRNA targeted against human HER2/neu may be valuable tools as anti proliferative agents that display activity against neoplastic cells at very low doses.
Geniale Abschalter für Brustkrebsgene : Hemmung des Turmorwachstums durch RNA-Interferenz (2005)
Strebhardt, Klaus ; Faltus, Timo
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