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Le Burkina Faso est situé dans la zone soudano-sahélienne où les cultures sont quelquefois aléatoires; dans ces conditions les plantes ligneuses occupent une place importante dans la vie socio-économique du pays. Pourtant, une sécheresses climatique persistante de ces dernières décennies a entraîné une mortalité massive de ces ligneux surtout dans la zone sahélienne. Cette forte dégradation des ressources naturelles est variable d’une zone géo-graphique à une autre pour des raisons diverses. Si les phénomènes généraux de cette dégradation de la végétation et leurs causes sont souvent décrits par de nombreux auteurs, il existe par contre très peu de connaissances détaillées et spécifiques en fonction des zones géographiques sur la menace qui pèse sur les différentes espèces. Dans l’absence de bases scientifiques fiables sur la végétation du passé pour chacune des régions qui permettrait ainsi d’établir avec précision la liste des espèces disparues, il est donc nécessaire de se baser sur la perception de la population rurale qui se trouve bien placée pour déceler tout changement dans la flore. Dans ce contexte, cette étude menée auprès des gourmantchés, vise surtout à identifier les espèces disparues ou celles en voie de disparition dans les différentes zones écologiques à l’est du Burkina Faso; en outre, elle aborde les raisons évoquées par ces populations pour expliquer cette menace qui pèse sur chacune des espèces. L’enquête a enfin contribué à connaître les solutions envisagées par ces dernières pour anéantir ou réduire la menace.
L’inventaire floristique du Burkina Faso est loin d’être clos. Les récentes investigations floristiques ont permis de recenser un grand nombre d’espèces qui n’avaient jamais été mentionnées sur la liste floristique nationale. Une partie de ces espèces sont d’ailleurs guinéocongolaises. Cet inventaire a concerné les aires classées et protégées situées dans le secteur Sudsoudanien. Les richesses floristiques se retrouvent concentrées dans ces aires classées et protégées car ce sont des formations qui jouissent d’une relative bonne gestion par rapport aux autres aires non protégées qui ont été fortement dégradées par l’homme. L’objectif de cette communication est de donner les informations issues de ces différentes investigations sur l’état de connaissance de la diversité végétale du Burkina Faso avec un accent particulier sur le mode de gestion et les utilisations de quelques espèces de la zone sud soudanienne.
Kagbeni and its irrigated oasis are surrounded by subdesert dwarf scrubland. In the present study, a list of 78 species of vascular plants is presented for Kagbeni and its immediate surroundings, supplemented with data on the distribution of the species within the entire Mustan District. The data are arrived from own investigations and the geobotanical literature. A phytogeographical analysis shows the prevalence of western over eastern elements. Species with a wide distribution in Eurasia, which constitute one third of the total flora of Kagbeni, are of great importance as weeds on arable fields and in ruderal places within the irrigated oasis. Their occurrence is closely related to human activity. Presumably, most of these weeds have reached the area under study in connection with agriculture a long time ago. Weeds from the New World, although recorded in other villages of Mustan District, have not been found in Kagbeni. The weed vegetation of Kagbeni is documented by nine vegetation releves, and is compared to releves from Jomsom and Mzrpha. A floristic gradient from south to north that has been detected by earlier investigations throughout the whole district can be reproduced at the local scale. With regard to the weed flora, the effects of different crops are minimal, compared to effects of altitude and other factors related to altitude.
Changes in settlement pattern and culture - the process of down-hill migration in Tula, Bauchi State
(1996)
The process of down-hill migration of the Tula people started during the 1920s and has not yet finished. The resulting present situation might give information how far terraces play any role in the economy, ecology and ideology of the Tula. Approaching this question from a socio-agricultural point of view some facts which indicate the pertaining or overcoming of traditional structures will be presented. In the following the land tenure system, the adoption of innovations and the role of women in agriculture will be discussed comparatively for Tula Wange and Tula Baule on the plateau, Fantami, which is generated by down-hill dwellers of Tula Wange, with its more or less bad farming conditions on shallow sandy soils and Kaltin, where the down migrants of Tula Baule settle in a more fertile area. Tula Wange numbers around 2000 households, Baule 1000, Fantami about 200 and Kaltin 350 of which the sample survey includes 15% in the plateau sites and 25% in the plain settlements.
Notre communication porte sur le thème suivant: "Anthropisation du couvert végétal dans la province de Namentenga et ses conséquences socioéconomiques: cas de la région de Tougouri, en zone subsahélienne" (centre-nord du Burkina Faso). A travers cette étude de cas, nous voulons attirer l'attention sur les problèmes environnementaux du Burkina Faso. En effet, les déficits pluviométriques cumulés, depuis quelques décennies, sont à l'origine de la dégradation des écosystèmes. La surexploitation des terres entraîne également l'épuisement des sols et la diminution des ressources végétales. Un telle situation n'est pas sans conséquence sur les activités socioéconomiques des populations. Le plan de notre communication sera donc comme suit: 1. Présentation du degré-carré de Tougouri, sur le plan physique 2. Dynamique du couvert végétal sous l'action anthropique, et les conséquences socioéconomiques 3. Perspectives d'avenir
La coupure que l'on constate dans les forêts denses africaines au niveau du Togo et du Bénin soulève de nombreuses questions sur les rapports forêt/savane au cours des derniers millénaires. Le projet Dahomey Gap vise à y répondre, par une approche pluridisciplinaire intégrant botanique et histoire de la végétation dans le Sud-Bénin et le Sud-Ouest du Nigéria. A partir de travaux déjà avancés sur la végétation du Bénin et des premières prospections palynologiques, quelques aspects du projet sont exposés ici: tels la caractérisation des différentes formations forestières du Bénin, les premiers résultats sur l'histoire holocène de la végétation du Sud-Bénin (mangroves, forêts, savanes) enregistrée depuis près de 7000 ans dans les lagunes.
Population structure was estimated in a continuous population of a small land snail (Trochoidea geyeri). Mark-recapture experiments and randomly amplified polymorphic DNA analyses indicate that the population structure can be described by the isolation by distance model of Wright (1946). Estimates of density and dispersal suggest a neighbourhood size of 70-208 individuals on an area of 13-21 m². A principal component analysis of the randomly amplified polymorphic DNA data reveals clinal variation of genetic composition across the population, as predicted by the neighbourhood concept. An analysis of molecular variance indicates substantial genetic structuring. Comparisons of the genetic distances, expressed as euclidean distances among individuals, versus the geographic distance between sampling sites yield a highly significant positive correlation (Mantel test: r = 0.567, p<0.0001). The revealed pattern of populational subdivision on a microgeographic scale seems to be one of the principal processes generating and maintaining genetic diversity within populations of small land gastropods.
In the present study the population genetic structure of the terrestrial snail Pomatias elegans was related to habitat structure on a microspatial scale. The genetic variability of 1607 individuals from 51 sampling sites in five different populations in Provence, France, was studied with an allozyme marker using population genetic methods, Mantel tests and spatial autocorrelation techniques were applied to different connectivity networks accounting for the structural features of the landscape. It is suggested that the population structure is, to a large extent, a function of the habitat quality, quantified as population density, and of the spatial arrangement of the habitat in the landscape and not of the geographical distance per se. In fragmented habitats, random genetic drift was the prevailing force for sampling sites separated by a few hundred meters.
Regenberg, Birgitte ; Grotkjær, Thomas ; Ole, Winther ; Fausbøll, Anders ; Åkesson, Mats ; Bro, Christoffer ; Hansen, Lars Kai ; Brunak, Søren ; Nielsen, Jens
Background: Growth rate is central to the development of cells in all organisms. However, little is known about the impact of changing growth rates. We used continuous cultures to control growth rate and studied the transcriptional program of the model eukaryote Saccharomyces cerevisiae, with generation times varying between 2 and 35 hours.
Results: A total of 5930 transcripts were identified at the different growth rates studied. Consensus clustering of these revealed that half of all yeast genes are affected by the specific growth rate, and that the changes are similar to those found when cells are exposed to different types of stress (>80% overlap). Genes with decreased transcript levels in response to faster growth are largely of unknown function (>50%) whereas genes with increased transcript levels are involved in macromolecular biosynthesis such as those that encode ribosomal proteins. This group also covers most targets of the transcriptional activator RAP1, which is also known to be involved in replication. A positive correlation between the location of replication origins and the location of growth-regulated genes suggests a role for replication in growth rate regulation.
Conclusion: Our data show that the cellular growth rate has great influence on transcriptional regulation. This, in turn, implies that one should be cautious when comparing mutants with different growth rates. Our findings also indicate that much of the regulation is coordinated via the chromosomal location of the affected genes, which may be valuable information for the control of heterologous gene expression in metabolic engineering.
Length variations of repetitive sequences in different AT-rich loop-coding regions of mitochondrial 16S rDNA in two gastropod species were discovered during intraspecific haplotype surveys. Examination of the discrete length variation of the basic repeat unit in a phylogenetic framework led to the conclusion of a microsatellite-like mutational dynamic. The observations suggest that the presence of a repetitive sequence structure alone is sufficient to trigger this dynamic.
I analysed the importance of shell size, shell shape, habitat preferences and availability, experienced climate, active dispersal and influence of Pleistocene glaciations for the range sizes of 37 Western Palaearctic Helicidae s.l. species for which a phylogeny was available. In both cross-species and phylogenetically controlled analyses, the range sizes were positively correlated to climatic tolerance, shell size, active dispersal and influence of Pleistocene glaciations. In addition, range sizes increased significantly with latitude. Multiple regression suggested that, predominantly, the influence of Pleistocene glaciations, tolerance to large annual temperature ranges and shell size influenced the distributional range sizes. Habitat preference, range and availability, active dispersal and shell shape explained no additional variance. The results suggest that the processes influencing species range size of the Helicidae s.l. are mainly related to the climatic shifts after the Pleistocene.
Leutner, Silke ; Czech, Christian ; Schindowski, Katharina ; Touchet, Nathalie ; Eckert, Anne ; Müller, Walter E.
Alzheimer's disease-related mutations in the presenilin-1 gene (PS1) are leading to an elevated production of neurotoxic beta-amyloid 1-42 and may additionally enhance oxidative stress. Here, we provide in vivo evidence indicating that brains of transgenic mice expressing different human Alzheimer-linked PS1 mutations exhibit a reduced activity of two antioxidant enzymes. For this purpose, mice transgenic for human PS1 and for single and multiple PS1 mutations were generated. Mice with multiple PS1 mutations showed a significantly decreased activity of the antioxidant enzymes Cu/Zn superoxide dismutase and glutathione reductase already at an age of 3-4 months. As expected, this effect was less pronounced for the mice with a single PS1 mutation. By contrast, animals bearing normal human PS1 showed significantly elevated enzyme activities relative to non-transgenic littermate controls.
Background Reliable taxonomic identification at the species level is the basis for many biological disciplines. In order to distinguish species, it is necessary that taxonomic characters allow for the separation of individuals into recognisable, homogeneous groups that differ from other such groups in a consistent way. We compared here the suitability and efficacy of traditionally used shell morphology and DNA-based methods to distinguish among species of the freshwater snail genus Radix (Basommatophora, Pulmonata). Results Morphometric analysis showed that shell shape was unsuitable to define homogeneous, recognisable entities, because the variation was continuous. On the other hand, the Molecularly defined Operational Taxonomic Units (MOTU), inferred from mitochondrial COI sequence variation, proved to be congruent with biological species, inferred from geographic distribution patterns, congruence with nuclear markers and crossing experiments. Moreover, it could be shown that the phenotypically plastic shell variation is mostly determined by the environmental conditions experienced. Conclusion Contrary to DNA-taxonomy, shell morphology was not suitable for delimiting and recognising species in Radix. As the situation encountered here seems to be widespread in invertebrates, we propose DNA-taxonomy as a reliable, comparable, and objective means for species identification in biological research.
Enhanced apoptosis and elevated levels of reactive oxygen species (ROS) play a major role in aging. In addition, several neurodegenerative diseases are associated with increased oxidative stress and apoptosis in neuronal tissue. Antioxidative treatment has neuro-protective effects. The aim of the present study was to evaluate changes of susceptibility to apoptotic cell death by oxidative stress in aging and its inhibition by the antioxidant Ginkgo biloba extract EGb761. We investigated basal and ROS-induced levels of apoptotic lymphocytes derived from the spleen in young (3 months) and old (24 months) mice. ROS were induced by 2-deoxy-D-ribose (dRib) that depletes the intracellular pool of reduced glutathione. Lymphocytes from aged mice accumulate apoptotic cells to a significantly higher extent under basal conditions compared to cells from young mice. Treatment with dRib enhanced this difference, implicating a higher sensitivity to ROS in aging. Apoptosis can be reduced in vitro by treatment with EGb761. In addition, mice were treated daily with 100mg/kg EGb761 per os over a period of two weeks. ROS-induced apoptosis was significantly reduced in the EGb761 group. Interestingly, this effect seemed to be more pronounced in old mice.
Apoptosis seems to be involved in immunosenescence associated with aging. Moreover, in lymphocytes (PBL) of patients with Alzheimer's disease, an increased susceptibility to the apoptotic pathway has been described possibly due to impaired protection of oxidative stress. Accordingly, it seemed to be of particular interest to investigate the contribution of normal aging to the susceptibility from human lymphocytes to programmed cell death. We could show that PBL from elderly individuals (>60 years) accumulate apoptosing cells to a significant higher extent in spontaneous and activation-induced cell death compared to younger controls (<35 years). Treatment with the oxidative stressor 2-deoxy-D-ribose or with agonistic-CD95-antibody pronounced this effect even more implicating a higher sensitivity to reactive oxygen species and a higher functional CD95 expression, respectively. In addition, expression of the activation markers HLA-DR and CD95 was significantly increased in CD3+-cells of aged subjects, while expression of CD25 did not seem to be affected by age. Expression of Bcl-2 was increased in aging and correlated with the number of apoptotic cells.
The relevance of physiological immune aging is of great interest with respect to determining disorders with pathologic immune function in aging individuals. In recent years, the relevance of changes in peripheral lymphocytes in age-associated neurologic diseases has become more evident. Due to the lack of immunological studies, covering more than one event after mitogenic activation, we envisaged a new concept in the present study, aiming to investigate several events, starting from T cell receptor (TCR) ligation up to T cell proliferation. In addition, we addressed the question whether changes are present in the subsets (CD4, CD8) with aging. Phosphorylation of tyrosine residues declines with increasing age in CD4+ cells. Fewer levels of CD69 positive cells after 4 h mitogenic activation, altered expression of cytokines (IL2, IFN-gamma and TNF-alpha; 22 h) and lower proliferation (72 h) were determined in aging. Moreover, it could be shown that CD8+ lymphocytes react more effectively to mitogenic stimulation with reference to CD69 expression and proliferation in both age groups (<35 and >60 years old). These data indicate that T cell activation, mediated by TCR engagement, is significantly impaired in aging and both subsets are affected. However, bypassing the TCR does not fully restore T cell function, indicating that there are more mechanisms involved than impaired signal transduction through TCR only. The results will be discussed in relation to their relevance in neurodegenerative and psychiatric disorders.
In vivo manipulation of interleukin-2 expression by a retroviral tetracycline (tet)-regulated system
(1999)
We have used the tetracycline (tet)-regulated system as described previously to evaluate the applicability of controlled gene expression in cancer gene therapy. As a model gene, we used the human interleukin-2 (IL-2) gene, which has been placed under the transcriptional control of the tetO/promoter. Human melanoma cells were transduced by two modified retroviral tet vectors containing the transactivator regulatory unit and the IL-2 gene driven by the tetO/promoter, respectively. In the absence of tet, IL-2 expression in the target cells was stable over several months. IL-2 production was in the range of 40 U/106 cells/24 hours. A fine tuning of IL-2 expression could be achieved by culturing the transduced cells with increasing doses of tet, whereby a concentration of 500 ng/mL tet in the culture medium abrogated IL-2 expression. Most importantly for clinical application, IL-2 expression by the transduced melanoma cells could also be regulated in vivo. When nu/nu mice were inoculated with the transduced tumor cells, they failed to develop tumors. Instead, the inhibition of IL-2 expression in the transduced tumor cells by oral administration of tet led to subcutaneous tumor growth; this growth rate was comparable with the growth rate of subcutaneously inoculated untransduced parental cells. The finding demonstrates the applicability of the tet-regulated system in cancer gene therapy.
Eckert, Anne ; Schindowski, Katharina ; Leutner, Silke ; Luckhaus, Christian ; Touchet, Nathalie ; Czech, Christian ; Müller, Walter E.
Many cases of early-onset inherited Alzheimer's disease (AD) are caused by mutations in the presenilin-1 (PS1) gene. Expression of PS1 mutations in cell culture systems and in primary neurons from transgenic mice increases their vulnerability to cell death. Interestingly, enhanced vulnerability to cell death has also been demonstrated for peripheral lymphocytes from AD patients. We now report that lymphocytes from PS1 mutant transgenic mice show a similar hypersensitivity to cell death as do peripheral cells from AD patients and several cell culture systems expressing PS1 mutations. The cell death-enhancing action of mutant PS1 was associated with increased production of reactive oxygen species and altered calcium regulation, but not with changes of mitochondrial cytochrome c. Our study further emphasizes the pathogenic role of mutant PS1 and may provide the fundamental basis for new efforts to close the gap between studies using neuronal cell lines transfected with mutant PS1, neurons from transgenic animals, and peripheral cells from AD patients. Copyright 2001 Academic Press.
Hamdane, Malika ; Bretteville, Alexis ; Sambo, Anne-Véronique ; Schindowski, Katharina ; Bégard, Séverine ; Delacourte, André ; Bertrand, Philippe ; Buée, Luc
In large models of neuronal cell death, there is a tight correlation between Cdk5 deregulation and cell-cycle dysfunction. However, pathways that link Cdk5 to the cell cycle during neuronal death are still unclear. We have investigated the molecular events that precede p25/Cdk5-triggered neuronal death using a neuronal cell line that allows inducible p25 expression. In this system, no sign of apoptosis was seen before 24 hours of p25 induction. Thus, at that time, cell-cycle-regulatory proteins were analysed by immunoblotting and some of them showed a significant deregulation. Interestingly, after time-course experiments, the earliest feature correlated with p25 expression was the phosphorylation of the retinoblastoma protein (Rb). Indeed, this phosphorylation was observed 6 hours after p25 induction and was abolished in the presence of a Cdk5 inhibitor, roscovitine, which does not inhibit the usual Rb cyclin-D kinases Cdk4 and Cdk6. Furthermore, analyses of levels and subcellular localization of Cdk-related cyclins did not reveal any change following Cdk5 activation, arguing for a direct effect of Cdk5 activity on Rb protein. This latter result was clearly demonstrated by in vitro kinase assays showing that the p25-Cdk5 complex in our cell system phosphorylates Rb directly without the need for any intermediary kinase activity. Hence, Rb might be an appropriate candidate that connects Cdk5 to cell-cycle deregulation during neuronal cell death.
Schindowski, Katharina ; Kratzsch, Tilmann ; Peters, Jürgen ; Steiner, Barbara ; Leutner, Silke ; Touchet, Natalie ; Maurer, Konrad ; Czech, Christian ; Pradier, Laurent ; Frölich, Lutz ; Müller, Walter E. ; Eckert, Anne
The identification of specific genetic (presenilin-1 [PS1] and amyloid precursor protein [APP] mutations) and environmental factors responsible for Alzheimer's disease (AD) has revealed evidence for a shared pathway of neuronal death. Moreover, AD-specific cell defects may be observed in many other nonneuronal cells (e.g., lymphocytes). Thus, lymphocytes may serve as a cellular system in which to study risk factors of sporadic, as well as genetic AD in vivo. The aim of our present study was to clarify whether lymphocytes bearing genetic or sporadic risk factors of AD share an increased susceptibility to cell death. Additionally we examined whether a cell typespecific vulnerability pattern was present and how normal aging, the main risk factor of sporadic AD, contributes to changes in susceptibility to cell death. Here, we report that lymphocytes affected by sporadic or genetic APP and PS1 AD risk factors share an increased vulnerability to cell death and exhibit a similar cell type-specific pattern, given that enhanced vulnerability was most strongly developed in the CD4+ T-cell subtype. In this paradigm, sporadic risk factors revealed the highest impact on cell type-specific sensitivity of CD4+ T cells to apoptosis. In contrast, normal aging results in an increased susceptibility to apoptosis of both, CD4+ and CD8+ T cells.