Medizin
Refine
Year of publication
Document Type
- Article (46)
- Book (6)
- Doctoral Thesis (1)
Has Fulltext
- yes (53)
Is part of the Bibliography
- no (53)
Keywords
- environmental tobacco smoke (4)
- particulate matter (4)
- IAPs (2)
- XIAP (2)
- additives (2)
- cytotoxicity (2)
- (novel) brominated flame retardants ((N)BFR) (1)
- AML – acute myeloid leukemia (1)
- ATP-citrate lyase (1)
- Adenocarcinoma (1)
- Autophagy (1)
- Biochemistry (1)
- CRISPR/Cas9 (1)
- CV9202 (1)
- Cancer epidemiology (1)
- Cas9 inhibitor (1)
- Cell biology (1)
- Chromatin and Epigenetics (1)
- Constrained posture (1)
- Cornea (1)
- Cuela (1)
- Dentist (1)
- Diabetes (1)
- Disease burden (1)
- E3 ligase (1)
- Endoplasmic reticulum (1)
- Entzündung (1)
- Enzyme mechanisms (1)
- Eyes (1)
- Funding (1)
- Gene Regulation (1)
- H3 receptor (1)
- Incidence (1)
- Kinematic analysis (1)
- LIR interaction, (1)
- Mitochondrial proteins (1)
- Mucopolysaccharidoses (1)
- Musculoskeletal disorder (1)
- Opacity (1)
- Ophthalmology (1)
- Optical lenses (1)
- PPAR (Peroxisome proliferator-activated receptors) (1)
- PROTAC (1)
- Polyphenole (1)
- Proteasome (1)
- RNActive (1)
- Rac1 (1)
- Research article (1)
- Research trends (1)
- Reticulon (1)
- RhoA (1)
- RhoGTPases (1)
- Rtn3 (1)
- Selective autophagy (1)
- Stilbene (1)
- Ubiquitination (1)
- Visual acuity (1)
- Visual impairments (1)
- X-ray crystallography (1)
- acetyl-CoA (1)
- air pollution (1)
- airborne bacteria (1)
- allergy (1)
- amoeboid (1)
- anticonvulsant activity (1)
- apoptosis (1)
- aromatics (1)
- asthma (1)
- atopy (1)
- automatic environmental tobacco smoke emitter (1)
- autophagy (1)
- cIAP1 (1)
- cIAP1 BIR (1)
- car indoor (1)
- carbon oxides (COx) (1)
- cell migration (1)
- cell proliferation (1)
- chemical-induced proximity (1)
- chronic myeloid leukemia (1)
- cigarette strength (1)
- collagen VI (1)
- costimulation (1)
- cytarabin (1)
- fungi (1)
- genetic predisposition (1)
- genome editing (1)
- high molecular weight plasticizer (1)
- histamine (1)
- histone acetylation (1)
- humectant agents (1)
- immunity (1)
- in-cabin exposure (1)
- inhibitors (1)
- isomeric antagonists (1)
- leukotriene (1)
- local radiotherapy (1)
- mRNA vaccine (1)
- macrophage (1)
- macrophage polarization (1)
- maternal tobacco smoke (1)
- menthol (1)
- mesenchymal (1)
- metabolic reprogramming (1)
- metabolism (1)
- metastases (1)
- microenvironment (1)
- migration (1)
- mitogenic effects (1)
- mobile air quality study (1)
- muscular dystrophies (1)
- nitrogen oxides (NOx) (1)
- non-small cell lung cancer (1)
- organophosphate flame retardants (OFR) (1)
- particle size distribution (1)
- particulate matter (PM) (1)
- passive smoke (1)
- phage display (1)
- plasticity (1)
- prostaglandins (1)
- proteomics (1)
- second-hand smoke (1)
- selective autophagy receptor (1)
- short linear motifs (SLiMs) (1)
- size of cigarettes (1)
- skeletal muscle (1)
- smoking in pregnancy (1)
- spermidine (1)
- stereoselectivity (1)
- traffic emissions (1)
- tumour (1)
- tyrosine kinase inhibitors. (1)
- ubiquitin (1)
- ventilation modes (1)
- volatile organic compounds (VOC) (1)
- wheezing (1)
- π-clamp (1)
Institute
- Biochemie und Chemie (53) (remove)
Poster presentation NO-sensitive guanylyl cyclases (soluble guanylyl cyclase, sGC) are among the key regulators of intracellular cGMP concentration. The mechanisms underlying NO-mediated activation of sGC are quite well understood, however, little is known about the fine-tuning of sGC activity through alternative mechanisms such as protein phosphorylation. Several reports have demonstrated the reversible phosphorylation of sGC on serine/threonine residues, and it has been speculated, though not experimentally proven, that sGC might also be phosphorylated on tyrosine residues. Using broad-spectrum phosphatase inhibitors we were able to demonstrate tyrosine phosphorylation at Tyr192 of the beta 1 subunit of human sGC in COS1 cells. This residue forms part of a sequence segment (YEDL) representing a preferential binding site for SH2 domains of Src-like kinases. Pull-down assays and co-immunoprecipitation experiments showed that Src can indeed bind via its SH2 domain to pTyr192 of beta 1 indicating that tyrosine phosphorylation of sGC may be followed by recruitment of Src-like kinases to the phosphorylated beta 1 subunit. In support of this hypothesis, immunofluorescence studies showed a colocalization of overexpressed sGC and Src at the plasma membrane of COS1 and Hela cells. Together, our results point to an unexpected crosstalk between tyrosine kinase pathway(s) and the NO/cGMP signalling cascade which may result in translocation of the predominantly cytosolic sGC to the cytosolic face of the plasma membrane.
Background: Microarray analysis still remains a powerful tool to identify new components of the transcriptosome and it has helped to increase the knowledge of targets triggered by stress conditions such as hypoxia and nitric oxide. However, analysis of transcriptional regulatory events remain elusive due to the contribution of altered mRNA stability to gene expression patterns, as well as changes in the half-life of mRNAs, which influence mRNA expression levels and their turn over rates. To circumvent these problems, we have focused on the analysis of newly transcribed (nascent) mRNAs by nuclear run on (NRO), followed by microarray analysis. Result: We identified 188 genes that were significantly regulated by hypoxia, 81 genes were affected by nitric oxide, and 292 genes were induced by the co-treatment of macrophages with both NO and hypoxia. Fourteen genes (Bnip3, Ddit4, Vegfa, Trib3, Atf3, Cdkn1a, Scd1, D4Ertd765e, Sesn2, Son, Nnt, Lst1, Hps6 and Fxyd5) were common to hypoxia and/or nitric oxide treatments, but with different levels of expression. We observed that 166 transcripts were regulated only when cells were co-treated with hypoxia and NO but not with either treatment alone, pointing to the importance of a crosstalk between hypoxia and NO. In addition, both array and proteomics data supported a consistent repression of hypoxia regulated targets by NO. Conclusion: By eliminating the interference of steady state mRNA in gene expression profiling, we increased the sensitivity of mRNA analysis and identified previously unknown hypoxia-induced targets. Gene analysis profiling corroborated the interplay between NO- and hypoxia-induced signalling.
Wirkungen von Heilpflanzen, Gewürzen, Tees und Lebensmitteln werden in der Naturheilkunde seit der Antike genutzt. Pharmakologisch wirksam sind in der Regel nur die sekundären Pflanzeninhaltsstoffe. Diese in den oft aus vielen Bestandteilen zusammengesetzten Naturstoffen aufzuspüren und ihren molekularbiologischen Wirkungsmechanismus im Körper aufzuklären, ist das Ziel eines Forschungsnetzwerks am Frankfurter ZAFES (Zentrum für Arzneimittelforschung, -Entwicklung und -Sicherheit). So konnten Pharmazeuten und Kliniker gemeinsam herausfinden, wie ein Bestandteil des Rotweins, das Resveratrol, vor Darmkrebs schützt. Die Inhaltsstoffe von Salbei und Rosmarin bieten vielversprechende Ausgangspunkte für neue Medikamente gegen Altersdiabetes. Weihrauch, Myrte und Johanniskraut enthalten Wirkstoffe, die Schlüsselenzyme für Entzündungsreaktionen – etwa bei rheumatischen Beschwerden – hemmen.