590 Tiere (Zoologie)
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A new species of Paracrobeles, P. kelsodunensis sp. nov. is described from the Kelso Dunes area, Mojave National Preserve, southern California. Paracrobeles kelsodunensis sp. nov. is particularly characterised by a body length of 469–626 μm in females and 463–569 μm in males; lateral field with four incisures, extending almost to tail terminus; three pairs of asymmetrical lips, separated by U-shaped primary axils with two long guarding processes, each lip usually with four tines along its margin; three long labial probolae, deeply bifurcated, with slender prongs without tines; metastegostom with a strong anteriorly directed dorsal tooth; pharyngeal corpus anteriorly spindle-shaped, posteriorly elongate bulbous with dilated lumen; spermatheca 24–87 μm long; postvulval uterine sac 60–133 μm long; vulva in a sunken area; spicules 33–38 μm long; and male tail with a 5–8 μm long mucro. The generic diagnosis is emended on the basis of recently described species and a key to the species of Paracrobeles is provided.
A new protocol to prepare material of Coccoidea (Insecta: Hemiptera) for observation with scanning electron microscopy is presented. This technique consists of cleaning samples (stored in 96% alcohol) with acetone and xylene, taking them directly to the hydration step, and subsequently dehydrating them in alcohol. Fixation in glutaraldehyde and post-fi xation in osmium tetroxide are omitted. The cleaning of the samples and the correct critical point dryer are very important.