Hematopoietic chimerism after allogeneic stem cell transplantation : a comparison of quantitative analysis by automated DNA sizing and fluorescent in situ hybridization

  • Background: Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is performed mainly in patients with high-risk or advanced hematologic malignancies and congenital or acquired aplastic anemias. In the context of the significant risk of graft failure after allo-HSCT from alternative donors and the risk of relapse in recipients transplanted for malignancy, the precise monitoring of posttransplant hematopoietic chimerism is of utmost interest. Useful molecular methods for chimerism quantification after allogeneic transplantation, aimed at distinguishing precisely between donor's and recipient's cells, are PCR-based analyses of polymorphic DNA markers. Such analyses can be performed regardless of donor's and recipient's sex. Additionally, in patients after sex-mismatched allo-HSCT, fluorescent in situ hybridization (FISH) can be applied. Methods: We compared different techniques for analysis of posttransplant chimerism, namely FISH and PCR-based molecular methods with automated detection of fluorescent products in an ALFExpress DNA Sequencer (Pharmacia) or ABI 310 Genetic Analyzer (PE). We used Spearman correlation test. Results: We have found high correlation between results obtained from the PCR/ALF Express and PCR/ABI 310 Genetic Analyzer. Lower, but still positive correlations were found between results of FISH technique and results obtained using automated DNA sizing technology. Conclusions: All the methods applied enable a rapid and accurate detection of post-HSCT chimerism.

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Author:Justyna Jólkowska, Anna Pieczonka, Tomasz Strabel, Dariusz Boruczkowski, Jacek Wachowiak, Peter BaderORCiDGND, Michal Witt
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/15642114
Parent Title (English):BMC blood disorders
Publisher:BioMed Central
Place of publication:London
Document Type:Article
Year of Completion:2005
Date of first Publication:2005/01/10
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2005/06/22
Issue:1, Art. 1
Page Number:6
First Page:1
Last Page:6
© 2005 Jólkowska et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Source:BMC Blood Disorders 2005, 5:1 , http://www.biomedcentral.com/content/pdf/1471-2326-5-1.pdf
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 2.0