Verteilung und enzymatische Hypermethylierung invers repetitiver DNA-Sequenzen in verschiedenen Mäuse-und menschlichen Zellen
Distribution and enzymic hypermethylation of inverted DNA repeats in different murine and human cells
- A specific class of DNA sequences, the inverted repetitive sequences, forms a double-stranded structure within a single linear polynucleotide chain in denatured DNA. The reassociation process is unimolecular and occurs very fast. Quantitative analyses have shown that these sequences com-E rise about 4-5% of the nuclear DNA of various mammalian cells (P815 mouse mastocytoma, Hela, L cells, Raji and Chang cells, and human embryonic hepatocytes) and are interspersed within sequences of other degrees of repetitiveness. After labeling the cells with L-[Metnyl-3H]methionine and [14C]deoxycytidine, relative rates of enzymic DNA methylation were computed on the basis of 3H and 14C radioactivities found in py rimidine residues of the nuclear DNA. The results indicate that DNA of inverted repetitive sequences is methylated to a level about 50% higher than the ordinary repetitive sequences and to about 300% higher than the unique and intermediary sequences. The biological function of the inverted repeats as well as the role of their enzymic hypermethyl ation is unknown.
Author: | Thomas L. J. BoehmGND, Dusan DrahovskyGND |
---|---|
URN: | urn:nbn:de:hebis:30:3-714068 |
DOI: | https://doi.org/10.1515/znc-1979-7-812 |
ISSN: | 0939-5075 |
ISSN: | 1865-7125 |
Parent Title (German): | Zeitschrift für Naturforschung, C |
Publisher: | Verlag der Zeitschrift für Naturforschung |
Place of publication: | Tübingen |
Document Type: | Article |
Language: | German |
Date of Publication (online): | 2014/06/02 |
Year of first Publication: | 1979 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2024/02/02 |
Tag: | Enzymatic DNA Methylation; Reassociation Kinetics |
Volume: | 34.1979 |
Issue: | 7-8 |
Page Number: | 7 |
First Page: | 558 |
Last Page: | 564 |
Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0 |