Enzymatic preparation of 14C-labeled phytoene, squalene, and geranylgeranyl pyrophosphate from [2-14C]mevalonic ccid
- Methods are described for an enzymatic preparation of 14C-labeled terpenoids. With a cell-free system of a white mutant of Phycomyces blakesleeanus (Mucoraceae) [14C]squalene and [14C- cis]phytoene can be synthesized from [2-14C]mevalonate. The application of norflurazon, a phenyl- pyridazinone herbicide, helps to increase the yield of squalene. Furthermore, the liquid endosperm of Echinocystis lobata (Cucurbitaceae) was used for the formation of either [14C(-)]kaurene from [14C]mevalonic acid or [14C-/ra/w]geranylgeranyl pyrophosphate in the presence of Amo 1618. The hydrocarbons formed were purified by alumina-column chromatography and preparative thin-layer chromatography (TLC). Geranylgeranyl pyrophosphate was separated by DE-column chromatography followed by TLC.
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| Author: | Gerhard SandmannORCiD, Wilhelm HilgenbergGND, Peter BögerGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-713935 |
| DOI: | https://doi.org/10.1515/znc-1980-11-1210 |
| ISSN: | 0939-5075 |
| ISSN: | 1865-7125 |
| Parent Title (German): | Zeitschrift für Naturforschung, C |
| Publisher: | Verlag der Zeitschrift für Naturforschung |
| Place of publication: | Tübingen |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2014/06/02 |
| Year of first Publication: | 1980 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2024/02/01 |
| Tag: | 14C-Labeled Terpenoids; Geranylgeranyl Pyrophosphate; Kaurene; Squalene; cis`-Phytoene |
| Volume: | 35.1980 |
| Issue: | 11-12 |
| Page Number: | 4 |
| First Page: | 927 |
| Last Page: | 930 |
| Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0 |
