The branching code: A model of actin-driven dendrite arborization
- The cytoskeleton is crucial for defining neuronal-type-specific dendrite morphologies. To explore how the complex interplay of actin-modulatory proteins (AMPs) can define neuronal types in vivo, we focused on the class III dendritic arborization (c3da) neuron of Drosophila larvae. Using computational modeling, we reveal that the main branches (MBs) of c3da neurons follow general models based on optimal wiring principles, while the actin-enriched short terminal branches (STBs) require an additional growth program. To clarify the cellular mechanisms that define this second step, we thus concentrated on STBs for an in-depth quantitative description of dendrite morphology and dynamics. Applying these methods systematically to mutants of six known and novel AMPs, we revealed the complementary roles of these individual AMPs in defining STB properties. Our data suggest that diverse dendrite arbors result from a combination of optimal-wiring-related growth and individualized growth programs that are neuron-type specific.
Author: | Tomke StürnerORCiDGND, André Ferreira CastroORCiDGND, Maren PhilippsORCiD, Hermann CuntzORCiDGND, Gaia TavosanisORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-783155 |
DOI: | https://doi.org/10.1016/j.celrep.2022.110746 |
ISSN: | 2211-1247 |
Parent Title (English): | Cell Reports |
Publisher: | Elsevier |
Place of publication: | Amsterdam |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2022/04/26 |
Date of first Publication: | 2022/04/26 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2023/10/30 |
Tag: | Drosophila; actin; actin-modulatory proteins; computational modeling; dendrite; dendritic arborization neurons; morphometrics; neuron; optimal wiring; time-lapse imaging |
Volume: | 39 |
Issue: | 4, art. 110746 |
Article Number: | 110746 |
Page Number: | 24 |
HeBIS-PPN: | 515839086 |
Institutes: | Wissenschaftliche Zentren und koordinierte Programme / Frankfurt Institute for Advanced Studies (FIAS) |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |