Click chemistry facilitates direct labelling and super-resolution imaging of nucleic acids and proteins
- We demonstrate high-density labelling of cellular DNA and RNA using click chemistry and perform confocal and super-resolution microscopy. We visualize the crescent and ring-like structure of densely packed RNA in nucleoli. We further demonstrate click chemistry with unnatural amino acids for super-resolution imaging of outer-membrane proteins of E. coli.
Author: | Anika Raulf, Christoph Klaus SpahnORCiDGND, Patrick Zessin, Kieran Finan, Stefan Bernhardt, Alexander HeckelORCiDGND, Mike HeilemannORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-450166 |
DOI: | https://doi.org/10.1039/C4RA01027B |
ISSN: | 2046-2069 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/25580242 |
Parent Title (English): | RSC Advances |
Publisher: | RSC Publishing |
Place of publication: | London |
Document Type: | Article |
Language: | English |
Year of Completion: | 2014 |
Date of first Publication: | 2014/06/27 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2017/12/14 |
Volume: | 4 |
Issue: | 57 |
Page Number: | 5 |
First Page: | 30462 |
Last Page: | 30466 |
Note: | This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. |
HeBIS-PPN: | 430463235 |
Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe | |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung 3.0 |