Mitochondrial phospholipid homeostasis is regulated by the i-AAA protease PaIAP and affects organismic aging
- Mitochondria are ubiquitous organelles of eukaryotic organisms with a number of essential functions, including synthesis of iron-sulfur clusters, amino acids, lipids, and adenosine triphosphate (ATP). During aging of the fungal aging model Podospora anserina, the inner mitochondrial membrane (IMM) undergoes prominent morphological alterations, ultimately resulting in functional impairments. Since phospholipids (PLs) are key components of biological membranes, maintenance of membrane plasticity and integrity via regulation of PL biosynthesis is indispensable. Here, we report results from a lipidomic analysis of isolated mitochondria from P. anserina that revealed an age-related reorganization of the mitochondrial PL profile and the involvement of the i-AAA protease PaIAP in proteolytic regulation of PL metabolism. The absence of PaIAP enhances biosynthesis of characteristic mitochondrial PLs, leads to significant alterations in the acyl composition of the mitochondrial signature PL cardiolipin (CL), and induces mitophagy. These alterations presumably cause the lifespan increase of the PaIap deletion mutant under standard growth conditions. However, PaIAP is required at elevated temperatures and for degradation of superfluous CL synthase PaCRD1 during glycolytic growth. Overall, our study uncovers a prominent role of PaIAP in the regulation of PL homeostasis in order to adapt membrane plasticity to fluctuating environmental conditions as they occur in nature.
Author: | Timo LöserORCiD, Aljoscha JoppeGND, Andrea HamannORCiD, Heinz D. OsiewaczORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-816614 |
DOI: | https://doi.org/10.3390/cells10102775 |
ISSN: | 2073-4409 |
Parent Title (English): | Cells |
Publisher: | MDPI |
Place of publication: | Basel |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2021/10/16 |
Date of first Publication: | 2021/10/16 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2024/01/23 |
Tag: | P. anserina; PaCRD1; PaIAP; aging; lipid metabolism; mitochondria |
Volume: | 10 |
Issue: | 10, art. 2775 |
Article Number: | 2775 |
Page Number: | 21 |
First Page: | 1 |
Last Page: | 21 |
Note: | This research was funded by the German Federal State of Hesse as part of the LOEWE Main Research Focus DynaMem. |
Institutes: | Biowissenschaften |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |