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Biofabrication of SDF-1 functionalized 3D-printed cell-free scaffolds for bone tissue regeneration
- Large segmental bone defects occurring after trauma, bone tumors, infections or revision surgeries are a challenge for surgeons. The aim of our study was to develop a new biomaterial utilizing simple and cheap 3D-printing techniques. A porous polylactide (PLA) cylinder was printed and functionalized with stromal-derived factor 1 (SDF-1) or bone morphogenetic protein 7 (BMP-7) immobilized in collagen type I. Biomechanical testing proved biomechanical stability and the scaffolds were implanted into a 6 mm critical size defect in rat femur. Bone growth was observed via x-ray and after 8 weeks, bone regeneration was analyzed with µCT and histological staining methods. Development of non-unions was detected in the control group with no implant. Implantation of PLA cylinder alone resulted in a slight but not significant osteoconductive effect, which was more pronounced in the group where the PLA cylinder was loaded with collagen type I. Addition of SDF-1 resulted in an osteoinductive effect, with stronger new bone formation. BMP-7 treatment showed the most distinct effect on bone regeneration. However, histological analyses revealed that newly formed bone in the BMP-7 group displayed a holey structure. Our results confirm the osteoinductive character of this 3D-biofabricated cell-free new biomaterial and raise new options for its application in bone tissue regeneration.
Verfasserangaben: | Alina Lauer, Philipp Wolf, Dorothea Mehler, Hermann Götz, Mehmet Rüzgar, Andreas Baranowski, Dirk HenrichORCiDGND, Pol Maria Rommens, Ulrike Ritz |
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URN: | urn:nbn:de:hebis:30:3-544607 |
DOI: | https://doi.org/10.3390/ijms21062175 |
Pubmed-Id: | https://pubmed.ncbi.nlm.nih.gov/32245268 |
Titel des übergeordneten Werkes (Englisch): | International journal of molecular sciences |
Verlag: | MDPI |
Verlagsort: | Basel |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Veröffentlichung (online): | 21.03.2020 |
Datum der Erstveröffentlichung: | 21.03.2020 |
Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Datum der Freischaltung: | 09.04.2020 |
Freies Schlagwort / Tag: | 3D printed cell-free scaffold; bone tissue regeneration; collagen type I; in vivo model of critical size defects; polylactide; stromal-derived factor 1 |
Jahrgang: | 21 |
Ausgabe / Heft: | 2175 |
Seitenzahl: | 17 |
Bemerkung: | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
HeBIS-PPN: | 463907469 |
Institute: | Medizin / Medizin |
DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Lizenz (Deutsch): | Creative Commons - Namensnennung 4.0 |