Igor Tsaur, Anika Noack, Jasmina Makarević, Elsie Oppermann, Ana Maria da Silva Rosa Waaga, Martin Gasser, Hendrik Borgmann, Tanja Huesch, Kilian Gust, Michael Andreas Reiter, David Schilling, Georg Bartsch, Axel Haferkamp, Roman A. Blaheta
- Purpose: Prostate specific antigen is not reliable in diagnosing prostate cancer (PCa), making the identification of novel, precise diagnostic biomarkers important. Since chemokines are associated with more aggressive disease and poor prognosis in diverse malignancies, we aimed to investigate the diagnostic relevance of chemokines in PCa.
Materials and methods: Preoperative and early postoperative serum samples were obtained from 39 consecutive PCa patients undergoing radical prostatectomy. Serum from 15 healthy volunteers served as controls. Concentrations of CXCL12, CXCL13, CX3CL1, CCL2, CCL5, and CCL20 were measured in serum by Luminex. The expression activity of CXCR3, CXCR4, CXCR5, CXCR7, CXCL12, CXCL13, CX3CR1, CXCL1, CCR2, CCR5, CCR6, CCR7, CCL2, and CCL5 mRNA was assessed in tumor and adjacent normal tissue of prostatectomy specimens by quantitative real-time polymerase chain reaction. The associations of these chemokines with clinical and histological parameters were tested.
Results: The gene expression activity of CCL2 and CCR6 was significantly higher in tumor tissue compared to adjacent normal tissue. CCL2 was also significantly higher in the blood samples of PCa patients, compared to controls. CCL5, CCL20, and CX3CL1 were lower in patient serum, compared to controls. CCR2 tissue mRNA was negatively correlated with the Gleason score and grading.
Conclusion: Chemokines are significantly modified during tumorigenesis of PCa, and CCL2 is a promising diagnostic biomarker.
MetadatenAuthor: | Igor TsaurORCiDGND, Anika Noack, Jasmina Makarević, Elsie Oppermann, Ana Maria da Silva Rosa Waaga, Martin Gasser, Hendrik Borgmann, Tanja Huesch, Kilian GustORCiDGND, Michael Andreas ReiterGND, David Schilling, Georg BartschGND, Axel HaferkampGND, Roman A. BlahetaORCiD |
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URN: | urn:nbn:de:hebis:30:3-373639 |
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DOI: | https://doi.org/10.4143/crt.2014.015 |
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ISSN: | 2005-9256 |
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ISSN: | 1598-2998 |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/25483747 |
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Parent Title (English): | Cancer research and treatment |
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Publisher: | Korean Cancer Association |
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Place of publication: | Seoul |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2015/04/25 |
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Date of first Publication: | 2014/10/13 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2015/04/25 |
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Tag: | Biological markers; Chemokine CCL2; Chemokines; Diagnosis; Prostatic neoplasms |
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Volume: | 47 |
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Issue: | 2 |
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Page Number: | 7 |
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First Page: | 306 |
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Last Page: | 312 |
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Note: | Copyright © 2015 by the Korean Cancer Association. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/)which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
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HeBIS-PPN: | 369146581 |
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Institutes: | Medizin / Medizin |
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Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell 3.0 |
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