Chemoresistance acquisition induces a global shift of expression of aniogenesis-associated genes and increased pro-angogenic activity in neuroblastoma cells
- Background Chemoresistance acquisition may influence cancer cell biology. Here, bioinformatics analysis of gene expression data was used to identify chemoresistance-associated changes in neuroblastoma biology. Results Bioinformatics analysis of gene expression data revealed that expression of angiogenesis-associated genes significantly differs between chemosensitive and chemoresistant neuroblastoma cells. A subsequent systematic analysis of a panel of 14 chemosensitive and chemoresistant neuroblastoma cell lines in vitro and in animal experiments indicated a consistent shift to a more pro-angiogenic phenotype in chemoresistant neuroblastoma cells. The molecular mechanims underlying increased pro-angiogenic activity of neuroblastoma cells are individual and differ between the investigated chemoresistant cell lines. Treatment of animals carrying doxorubicin-resistant neuroblastoma xenografts with doxorubicin, a cytotoxic drug known to exert anti-angiogenic activity, resulted in decreased tumour vessel formation and growth indicating chemoresistance-associated enhanced pro-angiogenic activity to be relevant for tumour progression and to represent a potential therapeutic target. Conclusions A bioinformatics approach allowed to identify a relevant chemoresistance-associated shift in neuroblastoma cell biology. The chemoresistance-associated enhanced pro-angiogenic activity observed in neuroblastoma cells is relevant for tumour progression and represents a potential therapeutic target.
Author: | Martin MichaelisORCiDGND, Denise KlassertGND, Susanne BarthGND, Tatyana SuhanORCiD, Rainer BreitlingORCiDGND, Bernd Mayer, Nora HinschGND, Hans Wilhelm DoerrGND, Jindrich CinatlORCiDGND, Jindrich CinatlORCiDGND |
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URN: | urn:nbn:de:hebis:30-71518 |
DOI: | https://doi.org/10.1186/1476-4598-8-80 |
ISSN: | 1476-4598 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/19788758 |
Parent Title (English): | Molecular cancer |
Publisher: | Biomed Central |
Place of publication: | London |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2009/10/27 |
Date of first Publication: | 2009/09/29 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2009/10/27 |
Volume: | 8 |
Issue: | Art. 80 |
Page Number: | 14 |
First Page: | 1 |
Last Page: | 14 |
Note: | © 2009 Michaelis et al. , licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
Source: | Molecular Cancer 2009, 8:80 ; doi:10.1186/1476-4598-8-80 ; http://www.molecular-cancer.com/content/8/1/80 |
HeBIS-PPN: | 219616558 |
Institutes: | Medizin / Medizin |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Licence (German): | Creative Commons - Namensnennung 2.0 |