Yvonne Volz, David Koschut, Alexandra Matzke-Og, Marina Dietz, Christos Karathanasis, Ludovic Richert, Moritz G. Wagner, Yves Mély, Mike Heilemann, Hartmut H. Niemann, Véronique Orian-Rousseau
- CD44v6, a member of the CD44 family of transmembrane glycoproteins is a co-receptor for two receptor tyrosine kinases (RTKs), Met and VEGFR-2 (vascular endothelial growth factor receptor 2). CD44v6 is not only required for the activation of these RTKs but also for signalling. In order to understand the role of CD44v6 in Met and VEGFR-2 activation and signalling we tested whether CD44v6 binds to their ligands, HGF (hepatocyte growth factor) and VEGF (vascular endothelial growth factor), respectively. FACS analysis and cellular ELISA showed binding of HGF and VEGF only to cells expressing CD44v6. Direct binding of CD44v6 to HGF and VEGF was demonstrated in pull-down assays and the binding affinities were determined using MicroScale Thermophoresis, fluorescence correlation spectroscopy and fluorescence anisotropy. The binding affinity of CD44v6 to HGF is in the micromolar range in contrast with the high-affinity binding measured in the case of VEGF and CD44v6, which is in the nanomolar range. These data reveal a heparan sulfate-independent direct binding of CD44v6 to the ligands of Met and VEGFR-2 and suggest different roles of CD44v6 for these RTKs.
MetadatenAuthor: | Yvonne Volz, David Koschut, Alexandra Matzke-Og, Marina DietzORCiDGND, Christos KarathanasisORCiDGND, Ludovic Richert, Moritz G. Wagner, Yves Mély, Mike HeilemannORCiDGND, Hartmut H. Niemann, Véronique Orian-Rousseau |
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URN: | urn:nbn:de:hebis:30:3-445453 |
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DOI: | https://doi.org/10.1042/BSR20150093 |
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ISSN: | 1573-4935 |
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ISSN: | 0144-8463 |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/26181364 |
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Parent Title (English): | Bioscience reports |
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Publisher: | Portland Press |
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Place of publication: | Colchester |
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Document Type: | Article |
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Language: | English |
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Year of Completion: | 2015 |
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Date of first Publication: | 2015/08/07 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2017/12/07 |
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Tag: | CD44s; CD44v6; HGF; Met; VEGF; binding affinity |
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Volume: | 35 |
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Issue: | 4, e00236 |
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Page Number: | 16 |
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First Page: | 1 |
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Last Page: | 16 |
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Note: | © 2015 Authors This is an open access article published by Portland Press Limited and distributed under the Creative Commons Attribution Licence 3.0 |
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HeBIS-PPN: | 42782169X |
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Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
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Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - Namensnennung 3.0 |
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