Effects of flavonoid-induced oxidative stress on anti-H5N1 influenza A virus activity exerted by baicalein and biochanin A
- Background: Different flavonoids are known to interfere with influenza A virus replication. Recently, we showed that the structurally similar flavonoids baicalein and biochanin A inhibit highly pathogenic avian H5N1 influenza A virus replication by different mechanisms in A549 lung cells. Here, we investigated the effects of both compounds on H5N1-induced reactive oxygen species (ROS) formation and the role of ROS formation during H5N1 replication. Findings: Baicalein and biochanin A enhanced H5N1-induced ROS formation in A549 cells and primary human monocyte-derived macrophages. Suppression of ROS formation induced by baicalein and biochanin A using the antioxidant N-acetyl-L-cysteine strongly increased the anti-H5N1 activity of both compounds in A549 cells but not in macrophages. Conclusions: These findings emphasise that flavonoids induce complex pharmacological actions some of which may interfere with H5N1 replication while others may support H5N1 replication. A more detailed understanding of these actions and the underlying structure-activity relationships is needed to design agents with optimised anti-H5N1 activity.
Author: | Martin MichaelisORCiDGND, Patchima Sithisarn, Jindrich CinatlORCiDGND |
---|---|
URN: | urn:nbn:de:hebis:30:3-352812 |
DOI: | https://doi.org/10.1186/1756-0500-7-384 |
ISSN: | 1756-0500 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/24958200 |
Parent Title (English): | BMC Research Notes |
Publisher: | BioMed Central |
Place of publication: | London |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2014/10/31 |
Date of first Publication: | 2014/06/23 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2014/10/31 |
Tag: | H5N1; N-acetyl-L-cysteine; antiviral; baicalein; biochanin A; reactive oxygen species |
Volume: | 7 |
Issue: | Art. 384 |
Page Number: | 6 |
First Page: | 1 |
Last Page: | 6 |
Note: | © 2014 Michaelis et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
HeBIS-PPN: | 366632051 |
Institutes: | Medizin / Medizin |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung 4.0 |