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N-terminally myristoylated feline foamy virus Gag allows Env-independent budding of sub-viral particles
- Foamy viruses (FVs) are distinct retroviruses classified as Spumaretrovirinae in contrast to the other retroviruses, the Orthoretrovirinae. As a unique feature of FVs, Gag is not sufficient for sub-viral particle (SVP) release. In primate and feline FVs (PFV and FFV), particle budding completely depends on the cognate FV Env glycoproteins. It was recently shown that an artificially added N-terminal Gag myristoylation signal (myr-signal) overcomes this restriction in PFV inducing an Orthoretrovirus-like budding phenotype. Here we show that engineered, heterologous N-terminal myr-signals also induce budding of the distantly related FFV Gag. The budding efficiency depends on the myr-signal and its location relative to the N-terminus of Gag. When the first nine amino acid residues of FFV Gag were replaced by known myr-signals, the budding efficiency as determined by the detection of extracellular SVPs was low. In contrast, adding myr-signals to the intact N-terminus of FFV Gag resulted in a more efficient SVP release. Importantly, budding of myr-Gag proteins was sensitive towards inhibition of cellular N-myristoyltransferases. As expected, the addition or insertion of myr-signals that allowed Env-independent budding of FFV SVPs also retargeted Gag to plasma membrane-proximal sites and other intracellular membrane compartments. The data confirm that membrane-targeted FV Gag has the capacity of SVP formation.
Verfasserangaben: | Yang Liu, Yong-Boum Kim, Martin Löchelt |
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URN: | urn:nbn:de:hebis:30:3-244722 |
DOI: | https://doi.org/10.3390/v3112223 |
ISSN: | 1999-4915 |
Pubmed-Id: | https://pubmed.ncbi.nlm.nih.gov/22163342 |
Titel des übergeordneten Werkes (Englisch): | Viruses |
Verlag: | Molecular Diversity Preservation International (MDPI) |
Verlagsort: | Basel |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Jahr der Fertigstellung: | 2012 |
Datum der Erstveröffentlichung: | 14.11.2011 |
Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Datum der Freischaltung: | 13.06.2012 |
Psyndex-Schlagwort: | assembly; budding; feline foamy virus; myristoylation; release; retrovirus; sub-viral particles |
Jahrgang: | 3 |
Seitenzahl: | 15 |
Erste Seite: | 2223 |
Letzte Seite: | 2237 |
HeBIS-PPN: | 306528924 |
Institute: | Medizin / Medizin |
DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Sammlung Biologie / Sondersammelgebiets-Volltexte | |
Lizenz (Deutsch): | Creative Commons - Namensnennung 3.0 |