Biosynthesis of fucoxanthin and diadinoxanthin and function of initial pathway genes in Phaeodactylum tricornutum
- The biosynthesis pathway to diadinoxanthin and fucoxanthin was elucidated in Phaeodactylum tricornutum by a combined approach involving metabolite analysis identification of gene function. For the initial steps leading to β-carotene, putative genes were selected from the genomic database and the function of several of them identified by genetic pathway complementation in Escherichia coli. They included genes encoding a phytoene synthase, a phytoene desaturase, a ζ-carotene desaturase, and a lycopene β-cyclase. Intermediates of the pathway beyond β-carotene, present in trace amounts, were separated by TLC and identified as violaxanthin and neoxanthin in the enriched fraction. Neoxanthin is a branching point for the synthesis of both diadinoxanthin and fucoxanthin and the mechanisms for their formation were proposed. A single isomerization of one of the allenic double bounds in neoxanthin yields diadinoxanhin. Two reactions, hydroxylation at C8 in combination with a keto-enol tautomerization and acetylation of the 3′-HO group results in the formation of fucoxanthin.
Author: | Michael Dambek, Ulrike Eilers, Jürgen BreitenbachGND, Sabine Steiger, Claudia BüchelORCiD, Gerhard SandmannORCiD |
---|---|
URN: | urn:nbn:de:hebis:30:3-255945 |
DOI: | https://doi.org/10.1093/jxb/ers211 |
ISSN: | 1460-2431 |
ISSN: | 0022-0957 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/22888128 |
Parent Title (English): | The journal of experimental botany |
Publisher: | Univ. Press |
Place of publication: | Oxford |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2012/08/09 |
Date of first Publication: | 2012/08/09 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2012/08/15 |
Tag: | Phaeodactylum tricornutum; carotenogenic genes; complementation; diadinoxanthin; fucoxanthin; genetic pathway; neoxanthin |
Volume: | 63 |
Issue: | 15 |
Page Number: | 6 |
HeBIS-PPN: | 357876369 |
Institutes: | Biowissenschaften / Biowissenschaften |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik) |
Sammlungen: | Universitätspublikationen |
Sammlung Biologie / Sondersammelgebiets-Volltexte | |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell 3.0 |