Lisa Kornstädt, Sandra Pierre, Andreas Weigert, Stefanie Ebersberger, Tim J. Schäufele, Anja Kolbinger, Tobias Schmid, Jennifer Cohnen, Dominique Jeanette Thomas, Nerea Ferreirós Bouzas, Bernhard Brüne, Ingo Ebersberger, Klaus Scholich
- Next to their role in IgE-mediated allergic diseases and in promoting inflammation, mast cells also have antiinflammatory functions. They release pro- as well as antiinflammatory mediators, depending on the biological setting. Here we aimed to better understand the role of mast cells during the resolution phase of a local inflammation induced with the Toll-like receptor (TLR)-2 agonist zymosan. Multiple sequential immunohistology combined with a statistical neighborhood analysis showed that mast cells are located in a predominantly antiinflammatory microenvironment during resolution of inflammation and that mast cell-deficiency causes decreased efferocytosis in the resolution phase. Accordingly, FACS analysis showed decreased phagocytosis of zymosan and neutrophils by macrophages in mast cell-deficient mice. mRNA sequencing using zymosan-induced bone marrow-derived mast cells (BMMC) revealed a strong type I interferon (IFN) response, which is known to enhance phagocytosis by macrophages. Both, zymosan and lipopolysaccharides (LPS) induced IFN-β synthesis in BMMCs in similar amounts as in bone marrow derived macrophages. IFN-β was expressed by mast cells in paws from naïve mice and during zymosan-induced inflammation. As described for macrophages the release of type I IFNs from mast cells depended on TLR internalization and endosome acidification. In conclusion, mast cells are able to produce several mediators including IFN-β, which are alone or in combination with each other able to regulate the phagocytotic activity of macrophages during resolution of inflammation.
MetadatenVerfasserangaben: | Lisa KornstädtGND, Sandra PierreORCiD, Andreas WeigertORCiDGND, Stefanie EbersbergerORCiD, Tim J. Schäufele, Anja KolbingerORCiD, Tobias SchmidORCiDGND, Jennifer Cohnen, Dominique Jeanette ThomasORCiDGND, Nerea Ferreirós BouzasORCiDGND, Bernhard BrüneORCiD, Ingo EbersbergerORCiDGND, Klaus ScholichORCiD |
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URN: | urn:nbn:de:hebis:30:3-609493 |
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DOI: | https://doi.org/10.3389/fimmu.2020.607048 |
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ISSN: | 1664-3224 |
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Titel des übergeordneten Werkes (Englisch): | Frontiers in immunology |
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Verlag: | Frontiers Media |
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Verlagsort: | Lausanne |
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Dokumentart: | Wissenschaftlicher Artikel |
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Sprache: | Englisch |
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Datum der Veröffentlichung (online): | 12.02.2021 |
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Datum der Erstveröffentlichung: | 12.02.2021 |
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Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Datum der Freischaltung: | 25.05.2021 |
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Freies Schlagwort / Tag: | IFN-β; inflammation; mast cells; resolution; toll-like receptor |
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Jahrgang: | 11 |
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Ausgabe / Heft: | art. 607048 |
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Seitenzahl: | 15 |
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Erste Seite: | 1 |
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Letzte Seite: | 15 |
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HeBIS-PPN: | 480791856 |
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Institute: | Medizin |
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DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Open-Access-Publikationsfonds: | Medizin |
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Lizenz (Deutsch): | Creative Commons - Namensnennung 4.0 |
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