Ann Vanderheyden, Nathalie Smitz, Katrien De Wolf, Isra Deblauwe, Wouter Dekoninck, Kenny Meganck, Sophie Gombeer, Adwine Vanslembrouck, Jacobus De Witte, Anna Schneider, Ingrid Verlé, Marc DeMeyer, Thierry Backeljau, Ruth Müller, Wim van Bortel
- This survey reports on the DNA identification and occurrence of Culex torrentium and Cx. pipiens s.s. in Belgium. These native disease-vector mosquito species are morphologically difficult to separate, and the biotypes of Cx. pipiens s.s. are morphologically indistinguishable. Culex torrentium and Cx. pipiens s.s. were identified using the COI and ACE2 loci. We recorded 1248 Cx. pipiens s.s. and 401 Cx. torrentium specimens from 24 locations in Belgium (collected between 2017 and 2019). Culex pipiens biotypes pipiens and molestus, and their hybrids, were differentiated using fragment-size analysis of the CQ11 locus (956 pipiens and 227 molestus biotype specimens, 29 hybrids). Hybrids were observed at 13 out of 16 sympatric sites. These results confirm that both species are widespread in Belgium, but while Cx. torrentium revealed many COI haplotypes, Cx. pipiens s.s. showed only one abundant haplotype. This latter observation may either reflect a recent population-wide demographic or range expansion, or a recent bottleneck, possibly linked to a Wolbachia infection. Finally, new evidence is provided for the asymmetric but limited introgression of the molestus biotype into the pipiens biotype.
MetadatenAuthor: | Ann VanderheydenORCiD, Nathalie SmitzORCiD, Katrien De WolfORCiD, Isra DeblauweORCiD, Wouter DekoninckORCiD, Kenny MeganckORCiD, Sophie GombeerORCiD, Adwine VanslembrouckORCiD, Jacobus De Witte, Anna SchneiderORCiD, Ingrid Verlé, Marc DeMeyerORCiDGND, Thierry BackeljauORCiD, Ruth MüllerORCiD, Wim van BortelORCiD |
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URN: | urn:nbn:de:hebis:30:3-755165 |
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DOI: | https://doi.org/10.3390/d14060486 |
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ISSN: | 1424-2818 |
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Parent Title (English): | Diversity |
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Publisher: | MDPI |
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Place of publication: | Basel |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2022/06/15 |
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Date of first Publication: | 2022/06/15 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2023/09/11 |
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Tag: | Culex pipiens biotypes pipiens/molestus; DNA-based identification; cytochrome c oxidase I (COI); disease vectors; fragment-size analyses (ACE2; CQ11); hybrids |
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Volume: | 14 |
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Issue: | 6, art.486 |
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Article Number: | 486 |
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Page Number: | 12 |
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HeBIS-PPN: | 513135324 |
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Institutes: | Medizin |
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Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 59 Tiere (Zoologie) / 590 Tiere (Zoologie) |
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| 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |
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