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Properties of an antiserum against Streptomyces hydrogenans 20β-hydroxysteroid dehydrogenase

  • Antiserum against crystallized 20β-hydroxysteroid dehydrogenase from Streptomyces hydrogenans was used for different immunodiffusion and immunoprecipitation tests to quantify the bacterial enzyme in cell-free supernatants of the microorganism. After immunoprecipitation and gel electrophoresis the molecular weight of the subunits of 20β-hydroxysteroid dehydrogenase was calculated to be 27 300 ± 700.

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Metadaten
Author:Bernhard Lotz, Joachim BetzGND, Lothar TrägerGND
URN:urn:nbn:de:hebis:30:3-721037
DOI:https://doi.org/10.1515/znc-1976-5-613
ISSN:0939-5075
ISSN:1865-7125
Parent Title (German):Zeitschrift für Naturforschung, C
Publisher:Verlag der Zeitschrift für Naturforschung
Place of publication:Tübingen
Document Type:Article
Language:English
Date of Publication (online):2014/06/02
Year of first Publication:1976
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2024/01/31
Tag:20β-Hydroxysteroid Dehydrogenase; Immunodiffusion; Immunoprecipitation; Molecular Weight; Streptomyces hydrogenans
Volume:31.1976
Issue:5-6
Page Number:6
First Page:288
Last Page:291
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0