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Identification of Mucormycosis by Fluorescence In Situ Hybridization Targeting Ribosomal RNA in Tissue Samples

  • Mucormycosis is an invasive fungal infection associated with high mortality, partly due to delayed diagnosis and inadequate empiric therapy. As fungal cultures often fail to grow Mucorales, identification of respective hyphae in tissue is frequently needed for diagnosis but may be challenging. We studied fluorescence in situ hybridization (FISH) targeting specific regions of the fungal ribosomal RNA (rRNA) of Mucorales to improve diagnosis of mucormycosis from tissue samples. We generated a probe combination specifically targeting Mucorales. Probe specificity was verified in silico and using cultivated fungi. Mucorales hyphae in tissue of a mouse model demonstrated a bright cytoplasmatic hybridization signal. In tissue samples of patients with mucormycosis, a positive signal was seen in 7 of 12 (58.3%) samples. However, autofluorescence in 3 of 7 (42.9%) samples impaired the diagnostic yield. Subsequent experiments suggested that availability of nutrients and antifungal therapy may impact on the FISH signal obtained with Mucorales hyphae. Diagnosis of mucormycosis from tissue might be improved by rRNA FISH in a limited number of cases only. FISH signals may reflect different wphysiological states of fungi in tissue. Further studies are needed to define the value of FISH to diagnose mucormycosis from other clinical samples.

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Author:Jill Jasmine DalimotORCiD, Ilka Mc Cormick Smith, Jasmin Gerkrath, Sylvia HartmannORCiDGND, Oliver Andreas CornelyORCiDGND, Soo Chan LeeORCiD, Joseph HeitmanORCiDGND, Volker RickertsORCiDGND
URN:urn:nbn:de:hebis:30:3-828162
DOI:https://doi.org/10.3390/jof8030289
ISSN:2309-608X
Parent Title (English):Journal of Fungi
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Date of Publication (online):2022/03/11
Date of first Publication:2022/03/11
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2024/06/25
Tag:fluorescence; hybridization; in situ; mucormycosis; pathology
Volume:8
Issue:3, art. 289
Article Number:289
Page Number:10
First Page:1
Last Page:10
Note:
Funding Information: NIH/NIAID R01 AI39115-24
Note:
Funding Information: NIH/NIAID R01 AI50113-17
Note:
Funding Information: NIH/NIAID R03 AI119617
Institutes:Medizin
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International