Ruth Pia Dücker, Elisabeth Adam, Sarah Wirtz, Lucia Gronau, Yascha Khodamoradi, Fabian J. Eberhardt, Helena Donath, Desiree Gutmann, Maria J. G. T. Vehreschild, Kai Zacharowski, Hermann Kreyenberg, Andreas Geburtig-Chiocchetti, Stefan Zielen, Ralf Schubert
- A high incidence of thromboembolic events associated with high mortality has been reported in severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infections with respiratory failure. The present study characterized post-transcriptional gene regulation by global microRNA (miRNA) expression in relation to activated coagulation and inflammation in 21 critically ill SARS-CoV-2 patients. The cohort consisted of patients with moderate respiratory failure (n = 11) and severe respiratory failure (n = 10) at an acute stage (day 0–3) and in the later course of the disease (>7 days). All patients needed supplemental oxygen and severe patients were defined by the requirement of positive pressure ventilation (intubation). Levels of D-dimers, activated partial thromboplastin time (aPTT), C-reactive protein (CRP), and interleukin (IL)-6 were significantly higher in patients with severe compared with moderate respiratory failure. Concurrently, next generation sequencing (NGS) analysis demonstrated increased dysregulation of miRNA expression with progression of disease severity connected to extreme downregulation of miR-320a, miR-320b and miR-320c. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis revealed involvement in the Hippo signaling pathway, the transforming growth factor (TGF)-β signaling pathway and in the regulation of adherens junctions. The expression of all miR-320 family members was significantly correlated with CRP, IL-6, and D-dimer levels. In conclusion, our analysis underlines the importance of thromboembolic processes in patients with respiratory failure and emphasizes miRNA-320s as potential biomarkers for severe progressive SARS-CoV-2 infection.
MetadatenAuthor: | Ruth Pia DückerORCiDGND, Elisabeth AdamORCiDGND, Sarah Wirtz, Lucia GronauORCiD, Yascha KhodamoradiORCiD, Fabian J. Eberhardt, Helena DonathORCiDGND, Desiree GutmannORCiD, Maria J. G. T. VehreschildORCiDGND, Kai ZacharowskiORCiDGND, Hermann KreyenbergORCiDGND, Andreas Geburtig-ChiocchettiORCiDGND, Stefan ZielenORCiDGND, Ralf SchubertORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-633965 |
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DOI: | https://doi.org/10.3390/ijms221910351 |
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ISSN: | 1422-0067 |
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Parent Title (English): | International journal of molecular sciences |
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Publisher: | Molecular Diversity Preservation International |
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Place of publication: | Basel |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2021/09/26 |
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Date of first Publication: | 2021/09/26 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2022/01/25 |
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Tag: | CRP; D-dimer; SARS-CoV-2; lung disease; miRNA; respiratory failure |
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Volume: | 22 |
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Issue: | 19, art. 10351 |
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Page Number: | 14 |
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First Page: | 1 |
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Last Page: | 14 |
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Note: | This research was funded by Starke Lunge Foundation. Starke Lunge is a non-profitable foundation registered in Germany (Regierungspräsidium Darmstadt: AZ: I 1325d 04/11(6)-78) that supports research projects in pediatric rare lung diseases. |
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HeBIS-PPN: | 490981828 |
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Institutes: | Medizin / Medizin |
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Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
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Sammlungen: | Universitätspublikationen |
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Open-Access-Publikationsfonds: | Medizin |
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Licence (German): | Creative Commons - Namensnennung 4.0 |
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