FAM134B-RHD protein clustering drives spontaneous budding of asymmetric membranes
- Living cells constantly remodel the shape of their lipid membranes. In the endo-plasmic reticulum (ER), the reticulon homology domain (RHD) of the reticulophagy regulator 1 (RETR1/FAM134B) forms dense autophagic puncta that are associated with membrane removal by ER-phagy. In molecular dynamics (MD) simulations, we find that FAM134B-RHD spontaneously forms clusters, driven in part by curvature-mediated attraction. At a critical size, the FAM134B-RHD clusters induce the formation of membrane buds. The kinetics of budding depends sensitively on protein concentration and bilayer asymmetry. Our MD simulations shed light on the role of FAM134B-RHD in ER-phagy and show that membrane asymmetry can be used to modulate the kinetics barrier for membrane remodeling.
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| Author: | Marc SiggelORCiDGND, Ramachandra M. BhaskaraORCiD, Melanie K. MoesserORCiD, Ivan ĐikićORCiDGND, Gerhard HummerORCiD |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-728546 |
| DOI: | https://doi.org/10.1101/2021.01.04.425110 |
| Parent Title (English): | bioRxiv |
| Document Type: | Preprint |
| Language: | English |
| Date of Publication (online): | 2021/01/04 |
| Date of first Publication: | 2021/01/04 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2023/03/27 |
| Issue: | 2021.01.04.425110 |
| Page Number: | 16 |
| HeBIS-PPN: | 510009131 |
| Institutes: | Physik |
| Medizin | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - CC BY-ND - Namensnennung - Keine Bearbeitungen 4.0 International |
